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Identifier: 156937733

GI number: 156937733

Start: 834973

End: 836757

Strand: Direct

Name: lig

Synonym: Igni_0942

Alternate gene names: 156937733

Gene position: 834973-836757 (Clockwise)

Preceding gene: 156937732

Following gene: 156937735

Centisome position: 64.35

GC content: 56.64

Gene sequence:

>1785_bases
TTGAAGTTTTCGGACGTCGTAGACGCCCTCGAGAGGCTGGAGAGGACTACCTCGAGGACACAGATAGTCGCTATATTAAC
GAACTTATTTAAGAAAGTCATTGAAGAGAACAAAGATATAATAGATAAGGTGGTTTACTTCATCCAAGGCAAGCTCTGGC
CCGACTGGTACGGCTACCCGGAGATAGGGATCGGCGAGAAGGGGATAATAAAGGCCATCTCCTTGGCCGCTAACGTTAAG
GAGAAGGAAGTGGAAGGCTTGTATAAGCAGCTGGGGGACTTGGGCTTAGTCGCCGAAAGGTTGATGGCGAAGGCCCCAAA
GGGGGGCCTGATGATGTTTGTAAAGAAGAAGGAAGAGTTGACGTTCGAGAAGGTATACGAGACTTTGAAGAGGATAGCCT
TCATGCAAGGGGAGGGGAGCCGGGACCTCAAGATAAAGACGTTGGCGGGCCTGCTCAAGGAGGCCTCGCCCAAGGAGGCG
AAGTACATCGTTAGGTTCGTCCAAGGGAAGTTGCGCTTGGGCGTGGGTGACGCTTCCATAATAGAGGCTTTGGCCCACGT
GGCGGGAACGACCAAGGACGTCGTCGAAAGGGCCTACAACTTGAGGGCGGACCTGGGAGCGGTGGCTAAGATAGCGGTCA
CCGAGGGCCCCGAAGCGCTGAAGAGGGTAAGGCCGAAGCCCGGCGTCCCGGTCCGCCCAATGTTGGCGGAGAGGCTCAAC
GACCCTAAGGAGATATTGAAGAAGCTCGGTGGCAAGGGGCTGGCCGAGTACAAGTACGACGGTGAGAGGGCTCAGATCCA
CTTGCTCCCGGACGGAAAGGTGGTGATATTCTCGAGGAGGTTGGAGAACATAACTAGGTCCTATCCGGACGTCGTCCAGT
ACGCCAAGTCCGGCTTGAAGGCAAAGGAGGCAATAGTAGAGGGAGAGATAATTGCGGTCAACCCCGAGACCGGGGAGCCA
AGGCCATTCCAAGAGTTGATGAGGCGGAGGAGGAAGCACGACGTAGCCCTGGCGATGAGTGAGATACCGGTCAACGTGAA
GTTGTTTGACATTATATACGTGGACGGCGAGGACATGACTAACAAGCCCCTCCCCCTCAGGAGGAAGAGGTTGGAAGAAG
TAGTTGAGGAGAGCGAGGAGTTCTCCCTTTCCACGGCAAAGTTAGTAAGTACGCCCGAGGAGCTCGAGCAGTTCTTCCAC
CAGTCCATTTCCGAGGGACACGAGGGCTTGGTGGTGAAGGCCGTCCACGACAAGAGCGTCTACCAAGCTGGGGCCAGAGG
GTGGCTCTGGATAAAGTACAAGAAAGACTACAAGAGCGAAATGGTAGAGCCGGTCGACTTGGTAGTTGTAGGGGCCTTCT
ACGGCAGGGGTAGGAGGGGAGGCACCTTCGGGGCCTTGTTAGTCGCCGGCTACGACGAGAAGAGGGACGCCTTTGCGACG
GTCTGCAAGGTAGGCTCGGGGTTCAGCGACGAGGAGCTGGCGAGGCTCCCGGAGCTCTTGAAGCCGTACATAAGCGAGAC
CAAGCCTCCTAGGGTCATAAGCAACGTTAAGCCCGACGTGTGGGTGAGGCCGGCGCTGGTGGCGGAGATCATCGGGGCGG
AAATAACGCTGAGTCCTATTCACACTTGCGCTAAGGACGAGGTCAGCGCGGGGAGCGGGCTGGCGATACGCTTCCCGCGT
TTCATACGCTGGAGGCCCGACAAGGGGCCGGAGGACGCCACCACGTGTGGAGAGATAGTGGAGATGTACAAAAGCAGGCT
CAAGAAGGTCGAGGAACCTACGTGA

Upstream 100 bases:

>100_bases
CGAGCCCTCCCCGAGGCTGGGCAAGCCTTACAACCCTAAGGAGTTCTTAGAGACCGCTCCCCTCAATTAGCTCCTCTCAC
TGGAGCCCCGGGCCCGCGCG

Downstream 100 bases:

>100_bases
CGAGCTCTGCCAACCTCTTGGTCTCCCAAGCCAGCCTTATTTCCATGGCGACCCTCCGACCCAAGCTTATAGGCCTCCCG
AACTGCAACTTGGAGTACTG

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MKFSDVVDALERLERTTSRTQIVAILTNLFKKVIEENKDIIDKVVYFIQGKLWPDWYGYPEIGIGEKGIIKAISLAANVK
EKEVEGLYKQLGDLGLVAERLMAKAPKGGLMMFVKKKEELTFEKVYETLKRIAFMQGEGSRDLKIKTLAGLLKEASPKEA
KYIVRFVQGKLRLGVGDASIIEALAHVAGTTKDVVERAYNLRADLGAVAKIAVTEGPEALKRVRPKPGVPVRPMLAERLN
DPKEILKKLGGKGLAEYKYDGERAQIHLLPDGKVVIFSRRLENITRSYPDVVQYAKSGLKAKEAIVEGEIIAVNPETGEP
RPFQELMRRRRKHDVALAMSEIPVNVKLFDIIYVDGEDMTNKPLPLRRKRLEEVVEESEEFSLSTAKLVSTPEELEQFFH
QSISEGHEGLVVKAVHDKSVYQAGARGWLWIKYKKDYKSEMVEPVDLVVVGAFYGRGRRGGTFGALLVAGYDEKRDAFAT
VCKVGSGFSDEELARLPELLKPYISETKPPRVISNVKPDVWVRPALVAEIIGAEITLSPIHTCAKDEVSAGSGLAIRFPR
FIRWRPDKGPEDATTCGEIVEMYKSRLKKVEEPT

Sequences:

>Translated_594_residues
MKFSDVVDALERLERTTSRTQIVAILTNLFKKVIEENKDIIDKVVYFIQGKLWPDWYGYPEIGIGEKGIIKAISLAANVK
EKEVEGLYKQLGDLGLVAERLMAKAPKGGLMMFVKKKEELTFEKVYETLKRIAFMQGEGSRDLKIKTLAGLLKEASPKEA
KYIVRFVQGKLRLGVGDASIIEALAHVAGTTKDVVERAYNLRADLGAVAKIAVTEGPEALKRVRPKPGVPVRPMLAERLN
DPKEILKKLGGKGLAEYKYDGERAQIHLLPDGKVVIFSRRLENITRSYPDVVQYAKSGLKAKEAIVEGEIIAVNPETGEP
RPFQELMRRRRKHDVALAMSEIPVNVKLFDIIYVDGEDMTNKPLPLRRKRLEEVVEESEEFSLSTAKLVSTPEELEQFFH
QSISEGHEGLVVKAVHDKSVYQAGARGWLWIKYKKDYKSEMVEPVDLVVVGAFYGRGRRGGTFGALLVAGYDEKRDAFAT
VCKVGSGFSDEELARLPELLKPYISETKPPRVISNVKPDVWVRPALVAEIIGAEITLSPIHTCAKDEVSAGSGLAIRFPR
FIRWRPDKGPEDATTCGEIVEMYKSRLKKVEEPT
>Mature_594_residues
MKFSDVVDALERLERTTSRTQIVAILTNLFKKVIEENKDIIDKVVYFIQGKLWPDWYGYPEIGIGEKGIIKAISLAANVK
EKEVEGLYKQLGDLGLVAERLMAKAPKGGLMMFVKKKEELTFEKVYETLKRIAFMQGEGSRDLKIKTLAGLLKEASPKEA
KYIVRFVQGKLRLGVGDASIIEALAHVAGTTKDVVERAYNLRADLGAVAKIAVTEGPEALKRVRPKPGVPVRPMLAERLN
DPKEILKKLGGKGLAEYKYDGERAQIHLLPDGKVVIFSRRLENITRSYPDVVQYAKSGLKAKEAIVEGEIIAVNPETGEP
RPFQELMRRRRKHDVALAMSEIPVNVKLFDIIYVDGEDMTNKPLPLRRKRLEEVVEESEEFSLSTAKLVSTPEELEQFFH
QSISEGHEGLVVKAVHDKSVYQAGARGWLWIKYKKDYKSEMVEPVDLVVVGAFYGRGRRGGTFGALLVAGYDEKRDAFAT
VCKVGSGFSDEELARLPELLKPYISETKPPRVISNVKPDVWVRPALVAEIIGAEITLSPIHTCAKDEVSAGSGLAIRFPR
FIRWRPDKGPEDATTCGEIVEMYKSRLKKVEEPT

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=625, Percent_Identity=33.44, Blast_Score=320, Evalue=3e-87,
Organism=Homo sapiens, GI73747829, Length=491, Percent_Identity=31.3645621181263, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI73747844, Length=491, Percent_Identity=31.3645621181263, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI46255052, Length=636, Percent_Identity=24.2138364779874, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI23199993, Length=636, Percent_Identity=24.2138364779874, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI148539894, Length=636, Percent_Identity=24.2138364779874, Blast_Score=146, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71985269, Length=604, Percent_Identity=32.9470198675497, Blast_Score=325, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI71985278, Length=177, Percent_Identity=30.5084745762712, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320038, Length=612, Percent_Identity=32.6797385620915, Blast_Score=317, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6324578, Length=411, Percent_Identity=24.8175182481752, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24762424, Length=607, Percent_Identity=33.2784184514003, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI85815841, Length=556, Percent_Identity=27.8776978417266, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24641829, Length=506, Percent_Identity=24.5059288537549, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_IGNH4 (A8AB20)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435529.1
- ProteinModelPortal:   A8AB20
- SMR:   A8AB20
- STRING:   A8AB20
- GeneID:   5562329
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0942
- eggNOG:   arNOG04596
- HOGENOM:   HBG570821
- OMA:   EYQSICK
- ProtClustDB:   PRK01109
- BioCyc:   IHOS453591:IGNI_0942-MONOMER
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 66460; Mature: 66460

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 258-258 BINDING 256-256 BINDING 263-263 BINDING 279-279 BINDING 309-309 BINDING 349-349 BINDING 426-426 BINDING 432-432

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSDVVDALERLERTTSRTQIVAILTNLFKKVIEENKDIIDKVVYFIQGKLWPDWYGYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCC
EIGIGEKGIIKAISLAANVKEKEVEGLYKQLGDLGLVAERLMAKAPKGGLMMFVKKKEEL
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHC
TFEKVYETLKRIAFMQGEGSRDLKIKTLAGLLKEASPKEAKYIVRFVQGKLRLGVGDASI
CHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHCHHEECCCCHHH
IEALAHVAGTTKDVVERAYNLRADLGAVAKIAVTEGPEALKRVRPKPGVPVRPMLAERLN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCCCCCCCCHHHHHHCC
DPKEILKKLGGKGLAEYKYDGERAQIHLLPDGKVVIFSRRLENITRSYPDVVQYAKSGLK
CHHHHHHHHCCCCCCCEEECCCEEEEEECCCCCEEEEHHHHHHHHHCCHHHHHHHHHCCH
AKEAIVEGEIIAVNPETGEPRPFQELMRRRRKHDVALAMSEIPVNVKLFDIIYVDGEDMT
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHEEHHHCCCEEEEEEEEEECCCCCC
NKPLPLRRKRLEEVVEESEEFSLSTAKLVSTPEELEQFFHQSISEGHEGLVVKAVHDKSV
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCHHH
YQAGARGWLWIKYKKDYKSEMVEPVDLVVVGAFYGRGRRGGTFGALLVAGYDEKRDAFAT
HHCCCCCEEEEEECCCHHHHHCCCHHHEEEEHHHCCCCCCCCEEEEEEECCCHHHHHHHH
VCKVGSGFSDEELARLPELLKPYISETKPPRVISNVKPDVWVRPALVAEIIGAEITLSPI
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEECHHHHHHHHCCCEEEHHH
HTCAKDEVSAGSGLAIRFPRFIRWRPDKGPEDATTCGEIVEMYKSRLKKVEEPT
HHHHHHCCCCCCCCEEECCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKFSDVVDALERLERTTSRTQIVAILTNLFKKVIEENKDIIDKVVYFIQGKLWPDWYGYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCC
EIGIGEKGIIKAISLAANVKEKEVEGLYKQLGDLGLVAERLMAKAPKGGLMMFVKKKEEL
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHC
TFEKVYETLKRIAFMQGEGSRDLKIKTLAGLLKEASPKEAKYIVRFVQGKLRLGVGDASI
CHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHCHHEECCCCHHH
IEALAHVAGTTKDVVERAYNLRADLGAVAKIAVTEGPEALKRVRPKPGVPVRPMLAERLN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCCCCCCCCHHHHHHCC
DPKEILKKLGGKGLAEYKYDGERAQIHLLPDGKVVIFSRRLENITRSYPDVVQYAKSGLK
CHHHHHHHHCCCCCCCEEECCCEEEEEECCCCCEEEEHHHHHHHHHCCHHHHHHHHHCCH
AKEAIVEGEIIAVNPETGEPRPFQELMRRRRKHDVALAMSEIPVNVKLFDIIYVDGEDMT
HHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHEEHHHCCCEEEEEEEEEECCCCCC
NKPLPLRRKRLEEVVEESEEFSLSTAKLVSTPEELEQFFHQSISEGHEGLVVKAVHDKSV
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCHHH
YQAGARGWLWIKYKKDYKSEMVEPVDLVVVGAFYGRGRRGGTFGALLVAGYDEKRDAFAT
HHCCCCCEEEEEECCCHHHHHCCCHHHEEEEHHHCCCCCCCCEEEEEEECCCHHHHHHHH
VCKVGSGFSDEELARLPELLKPYISETKPPRVISNVKPDVWVRPALVAEIIGAEITLSPI
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEECHHHHHHHHCCCEEEHHH
HTCAKDEVSAGSGLAIRFPRFIRWRPDKGPEDATTCGEIVEMYKSRLKKVEEPT
HHHHHHCCCCCCCCEEECCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA