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The map label for this gene is leuC1 [H]

Identifier: 156937722

GI number: 156937722

Start: 824889

End: 826148

Strand: Direct

Name: leuC1 [H]

Synonym: Igni_0931

Alternate gene names: 156937722

Gene position: 824889-826148 (Clockwise)

Preceding gene: 156937721

Following gene: 156937725

Centisome position: 63.57

GC content: 60.24

Gene sequence:

>1260_bases
ATGCCGAAAACCATGGCAGAGAAGATACTCGGCAGGGCCAGCGGCAAGGACGTGAGTCCCGGAGAGATCGTAGAGGCCAA
TGTGGACTTAGCCATGGTTCACGACTTGACCGGCCCCCACGTGGTGGAGGTGGTTGAGGAGATAGCGGGGGAGGTTAGGG
TCTGGGACCCCGACAAGGTCGCCATAATATTCGACCACCACGTGCCCGCCGACAAGATAAGGGCGGCGGAGCTCCAAGCG
ATAATGAGGAAGGTGGCTAGGAAGCTCGGCATAAAGAAGTTCCACGACGTGGGGAGAGGGGGGATTTGCCACCAAGTGTT
GGTGGAGGAGCGCTACGCGAGGCCCGGGATGCTGATAGTAGGCGCGGACTCCCACACGGTGACCTCCGGGGCGGTGGGGG
CCTTCGCGACTGGTATCGGGGCGAGCGACATGGCCATGGTCTGGCTCACGGGCAAGCTCTGGTTCAAGGTGCCCGAGTCT
ATAAAAGTCAACGTTACGGGCGAGCTGCCCAAGGGCGTCTACGCGAAGGACGTAATACTTCATATAATAGGCACCGTAAC
CGTTGACGGCGCCACTTACAAGGCCGTGGAGTACCACGGAGACACAATCAAGAGGATGAACATAGCAGACAGGATGACGC
TGGCCAACATGAGCGTTGAGATGGGCGCCAAGGCAGGCATGGTCCCCGCAGACGAGGTTACGGTCAAGTTCTTCGAGGAG
GCCGGCATAAGCGTTAAGCCCTTCGGCCCCGACCCGGGAGCTCAGTACGAGGACGTTTGGGAGTTCGACGTGAGTAAGCT
GGAACCTCAAGTGGCGGTGCCCCACAGCGTGGACAACGTCGTCGCGGTGGGCGAGGTGGAGGGAACTCCCATCGACCAAG
CCTTCATAGGCAGCTGCACCAACGGGCGTTACGAGGACTTCGTGGAGGCGGCGAAGATACTGAAGGGGAGGAAGGTAGCT
CCCGGGGTTAGGTGCATAGCCATACCCGCCTCCTTGAAGCAGTACATGAGGCTACTCAAGGAAGGCATAATTGACATCTT
AACCGAGGCGGGCTGCTTCGTCGCCCACAGCACTTGTGGTCCGTGCCTCGGAGGACACTTAGGGGTGTTGGGGCCCGGCG
AGGTGGCGATATCCTCCAGCAACAGGAACTTCAAGGGCAGGATGGGCCACCCGGAGAGCAAGGTCTACTTAGCTAGCCCC
GCCACCGTAGCGGCAAGCGCGGTGGAGGGCAAGATAGCTGACCCGCGGAAGTACCTCTGA

Upstream 100 bases:

>100_bases
TAAGGGTGCAAGCTCTGAGGGCCGGAATGATGAACCGGTCTTAAGGCCGATCCGCGCAACACAAAAAATCCTCCTCCTCT
TCTCTCGCCGGGTCCTAATC

Downstream 100 bases:

>100_bases
GGTCCTCGGTGAGGCTAGGGGCTCTTCACCGCTCCGGACCGGCCTGGTGGTCCTCATCGGACCCCGAGGGCCGCGGCCAG
CTCGCGGGGGGATTTCAACA

Product: 3-isopropylmalate dehydratase, large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPKTMAEKILGRASGKDVSPGEIVEANVDLAMVHDLTGPHVVEVVEEIAGEVRVWDPDKVAIIFDHHVPADKIRAAELQA
IMRKVARKLGIKKFHDVGRGGICHQVLVEERYARPGMLIVGADSHTVTSGAVGAFATGIGASDMAMVWLTGKLWFKVPES
IKVNVTGELPKGVYAKDVILHIIGTVTVDGATYKAVEYHGDTIKRMNIADRMTLANMSVEMGAKAGMVPADEVTVKFFEE
AGISVKPFGPDPGAQYEDVWEFDVSKLEPQVAVPHSVDNVVAVGEVEGTPIDQAFIGSCTNGRYEDFVEAAKILKGRKVA
PGVRCIAIPASLKQYMRLLKEGIIDILTEAGCFVAHSTCGPCLGGHLGVLGPGEVAISSSNRNFKGRMGHPESKVYLASP
ATVAASAVEGKIADPRKYL

Sequences:

>Translated_419_residues
MPKTMAEKILGRASGKDVSPGEIVEANVDLAMVHDLTGPHVVEVVEEIAGEVRVWDPDKVAIIFDHHVPADKIRAAELQA
IMRKVARKLGIKKFHDVGRGGICHQVLVEERYARPGMLIVGADSHTVTSGAVGAFATGIGASDMAMVWLTGKLWFKVPES
IKVNVTGELPKGVYAKDVILHIIGTVTVDGATYKAVEYHGDTIKRMNIADRMTLANMSVEMGAKAGMVPADEVTVKFFEE
AGISVKPFGPDPGAQYEDVWEFDVSKLEPQVAVPHSVDNVVAVGEVEGTPIDQAFIGSCTNGRYEDFVEAAKILKGRKVA
PGVRCIAIPASLKQYMRLLKEGIIDILTEAGCFVAHSTCGPCLGGHLGVLGPGEVAISSSNRNFKGRMGHPESKVYLASP
ATVAASAVEGKIADPRKYL
>Mature_418_residues
PKTMAEKILGRASGKDVSPGEIVEANVDLAMVHDLTGPHVVEVVEEIAGEVRVWDPDKVAIIFDHHVPADKIRAAELQAI
MRKVARKLGIKKFHDVGRGGICHQVLVEERYARPGMLIVGADSHTVTSGAVGAFATGIGASDMAMVWLTGKLWFKVPESI
KVNVTGELPKGVYAKDVILHIIGTVTVDGATYKAVEYHGDTIKRMNIADRMTLANMSVEMGAKAGMVPADEVTVKFFEEA
GISVKPFGPDPGAQYEDVWEFDVSKLEPQVAVPHSVDNVVAVGEVEGTPIDQAFIGSCTNGRYEDFVEAAKILKGRKVAP
GVRCIAIPASLKQYMRLLKEGIIDILTEAGCFVAHSTCGPCLGGHLGVLGPGEVAISSSNRNFKGRMGHPESKVYLASPA
TVAASAVEGKIADPRKYL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=338, Percent_Identity=34.6153846153846, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI8659555, Length=490, Percent_Identity=25.9183673469388, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI41352693, Length=393, Percent_Identity=25.6997455470738, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1786259, Length=443, Percent_Identity=34.0857787810384, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787531, Length=480, Percent_Identity=27.9166666666667, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI87081781, Length=351, Percent_Identity=26.7806267806268, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI2367097, Length=461, Percent_Identity=23.2104121475054, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI32564738, Length=337, Percent_Identity=31.7507418397626, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25149337, Length=337, Percent_Identity=31.7507418397626, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25149342, Length=283, Percent_Identity=30.3886925795053, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=206, Percent_Identity=28.1553398058252, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=33.7028824833703, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321429, Length=449, Percent_Identity=30.0668151447661, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6323335, Length=339, Percent_Identity=33.0383480825959, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322261, Length=373, Percent_Identity=30.5630026809651, Blast_Score=155, Evalue=9e-39,
Organism=Drosophila melanogaster, GI28571643, Length=336, Percent_Identity=32.1428571428571, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI161076999, Length=373, Percent_Identity=28.9544235924933, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI281365315, Length=373, Percent_Identity=28.9544235924933, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI17864292, Length=373, Percent_Identity=28.9544235924933, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI17137564, Length=495, Percent_Identity=25.0505050505051, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24645686, Length=255, Percent_Identity=29.8039215686275, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44771; Mature: 44640

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTMAEKILGRASGKDVSPGEIVEANVDLAMVHDLTGPHVVEVVEEIAGEVRVWDPDKV
CCHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHHCCCEEEECCCEE
AIIFDHHVPADKIRAAELQAIMRKVARKLGIKKFHDVGRGGICHQVLVEERYARPGMLIV
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEE
GADSHTVTSGAVGAFATGIGASDMAMVWLTGKLWFKVPESIKVNVTGELPKGVYAKDVIL
ECCCCEECCCCHHHHHHCCCCCCEEEEEEECEEEEECCCCEEEEEECCCCCCCHHHHHHH
HIIGTVTVDGATYKAVEYHGDTIKRMNIADRMTLANMSVEMGAKAGMVPADEVTVKFFEE
HHHHHEEECCCEEEEEEECCCCHHHCCHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHH
AGISVKPFGPDPGAQYEDVWEFDVSKLEPQVAVPHSVDNVVAVGEVEGTPIDQAFIGSCT
CCCEEECCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCCEEEEECCCCCCCCHHHHCCCC
NGRYEDFVEAAKILKGRKVAPGVRCIAIPASLKQYMRLLKEGIIDILTEAGCFVAHSTCG
CCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
PCLGGHLGVLGPGEVAISSSNRNFKGRMGHPESKVYLASPATVAASAVEGKIADPRKYL
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PKTMAEKILGRASGKDVSPGEIVEANVDLAMVHDLTGPHVVEVVEEIAGEVRVWDPDKV
CHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHHCCCEEEECCCEE
AIIFDHHVPADKIRAAELQAIMRKVARKLGIKKFHDVGRGGICHQVLVEERYARPGMLIV
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEE
GADSHTVTSGAVGAFATGIGASDMAMVWLTGKLWFKVPESIKVNVTGELPKGVYAKDVIL
ECCCCEECCCCHHHHHHCCCCCCEEEEEEECEEEEECCCCEEEEEECCCCCCCHHHHHHH
HIIGTVTVDGATYKAVEYHGDTIKRMNIADRMTLANMSVEMGAKAGMVPADEVTVKFFEE
HHHHHEEECCCEEEEEEECCCCHHHCCHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHH
AGISVKPFGPDPGAQYEDVWEFDVSKLEPQVAVPHSVDNVVAVGEVEGTPIDQAFIGSCT
CCCEEECCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCCEEEEECCCCCCCCHHHHCCCC
NGRYEDFVEAAKILKGRKVAPGVRCIAIPASLKQYMRLLKEGIIDILTEAGCFVAHSTCG
CCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
PCLGGHLGVLGPGEVAISSSNRNFKGRMGHPESKVYLASPATVAASAVEGKIADPRKYL
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]