Click here to switch to the map view.

The map label for this gene is hisS [H]

Identifier: 156937672

GI number: 156937672

Start: 779635

End: 780924

Strand: Direct

Name: hisS [H]

Synonym: Igni_0879

Alternate gene names: 156937672

Gene position: 779635-780924 (Clockwise)

Preceding gene: 156937671

Following gene: 156937673

Centisome position: 60.09

GC content: 55.97

Gene sequence:

>1290_bases
TTGAAGCTGGACCCCGAGCCGCTCAGAGGGTTCAGAGACCTCTTCGGGGAAGAGGCGGAGGCGTTGGAGGCGGTCACCGA
GGAGGCGGAGAAGCTGGCTAGGAACTGGTCCTTTGAAAGGTGCTACTTACCCACGGTGGAGCGCTTCGAGCTCTTTGCCA
TGAAGAGCGGTGTGGAAATAGAGAGAAGTATGTACGTGTTCGTCGACAAGGGCGGAAGGAAGGTCACCCTCCGACCGGAG
GCCACGGCTTCCGCAGTGAGGGCTTACTTGAGGTTGTTGCGTCCGAGGCCCAAGCCGGTGAAGCTCTTCACAGTAGTTAA
CGTCTTCAGGTACGATGAGCCTCAGTTCGCGAGGTACAGGGAGTTCTACCAAGCCGACTTCGAGGTCTTCGGGGCCTCGT
CGCCGTACCAAGACTCGGAACTCTTGACCATGCTCCACGAGTACTACGAGAACTTGGGGCTCCCCCACGAGATACTCCTC
GGCAGCGTTAAGGCTCTGAGGGGCGTCCTGTCCGAGGAAGGCATAGAAGAGAGGGACCAAGACGTGGTCCTTCACTACGT
AGACAAAGGCATGATAGATAAGGCCTTGGAGCTTGTGGAGAACAAGGCGAGGAGGCCGGAGAGGGCCAAGGAGGCGGTCG
CCGTATTGTCGACCAAGGAGGGGGGCGAGGAGGTAATCAAAGAGGGGAAGGAGTTGATGGAGTCTTGGGGCTACAAGTAC
CGTTTCGAGGACTTAGAAGAGGTGTTGAAGTATCTCCCGGATGACGTGAAGAGTGTAATAAGGTTGAAGTTGGGCTTCGC
CCGCGGCTTGGCGTATTACACTGGTTTAATTTATGAAGTAAGGGTGCCGGGGTTCCCGGTGTCGGTGGCCGGAGGGGGGA
GGTACGACGCGCTCACGGAGGTTTACGGTTGGGAGAGGGTGCCGGCGACAGGCTTCGCCATAGGTTTGGACAGAACCGCC
CTAGCGTTGATGAAAGAGGGAAGGAGGTTCGTGTTCAGACCAACCGTCGCCGTGTTGCCCTTAGCCGAGGAGGCGAGGAA
GGTAGCCTTAGAGGTCCAGAGGGAGCTCGCCTCCTTAGACGTAAACTCCTTCTTGCTAACCGAACAGAAGAGTATTAAGA
AGGCACTTTCCTTTGCCTCTTCCATGAATGCTAAGGTTGCTATAATAATAGGGAAGAAGGAGGTAGAGGAGAATAAAGTT
ACCATAAAGAACTTGGAAAAGGGCCTCCAAGTGAGCTGCGGGCGGGCGGAGCTGTTGGACTGTTTGAGCGAGACGTTGAA
AACTCCGTGA

Upstream 100 bases:

>100_bases
TGTCCACAGTTATGCTCTACCAGCAGTTAATGCTAATAATGATGATTGTTGTTGTCCTAATTTTAGCGTTCTTAGTACTG
CGGGGCGGGTAGGCGAGGCC

Downstream 100 bases:

>100_bases
GCGAGAACAGCTCTCGGGGGACGCTCTTGAGTTTAGAGAAGCTGGGTCCCGGGAAGGACGCCCCCGAGGTCGTTAACGTA
GTGATAGAAATACCTATGGG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKLDPEPLRGFRDLFGEEAEALEAVTEEAEKLARNWSFERCYLPTVERFELFAMKSGVEIERSMYVFVDKGGRKVTLRPE
ATASAVRAYLRLLRPRPKPVKLFTVVNVFRYDEPQFARYREFYQADFEVFGASSPYQDSELLTMLHEYYENLGLPHEILL
GSVKALRGVLSEEGIEERDQDVVLHYVDKGMIDKALELVENKARRPERAKEAVAVLSTKEGGEEVIKEGKELMESWGYKY
RFEDLEEVLKYLPDDVKSVIRLKLGFARGLAYYTGLIYEVRVPGFPVSVAGGGRYDALTEVYGWERVPATGFAIGLDRTA
LALMKEGRRFVFRPTVAVLPLAEEARKVALEVQRELASLDVNSFLLTEQKSIKKALSFASSMNAKVAIIIGKKEVEENKV
TIKNLEKGLQVSCGRAELLDCLSETLKTP

Sequences:

>Translated_429_residues
MKLDPEPLRGFRDLFGEEAEALEAVTEEAEKLARNWSFERCYLPTVERFELFAMKSGVEIERSMYVFVDKGGRKVTLRPE
ATASAVRAYLRLLRPRPKPVKLFTVVNVFRYDEPQFARYREFYQADFEVFGASSPYQDSELLTMLHEYYENLGLPHEILL
GSVKALRGVLSEEGIEERDQDVVLHYVDKGMIDKALELVENKARRPERAKEAVAVLSTKEGGEEVIKEGKELMESWGYKY
RFEDLEEVLKYLPDDVKSVIRLKLGFARGLAYYTGLIYEVRVPGFPVSVAGGGRYDALTEVYGWERVPATGFAIGLDRTA
LALMKEGRRFVFRPTVAVLPLAEEARKVALEVQRELASLDVNSFLLTEQKSIKKALSFASSMNAKVAIIIGKKEVEENKV
TIKNLEKGLQVSCGRAELLDCLSETLKTP
>Mature_429_residues
MKLDPEPLRGFRDLFGEEAEALEAVTEEAEKLARNWSFERCYLPTVERFELFAMKSGVEIERSMYVFVDKGGRKVTLRPE
ATASAVRAYLRLLRPRPKPVKLFTVVNVFRYDEPQFARYREFYQADFEVFGASSPYQDSELLTMLHEYYENLGLPHEILL
GSVKALRGVLSEEGIEERDQDVVLHYVDKGMIDKALELVENKARRPERAKEAVAVLSTKEGGEEVIKEGKELMESWGYKY
RFEDLEEVLKYLPDDVKSVIRLKLGFARGLAYYTGLIYEVRVPGFPVSVAGGGRYDALTEVYGWERVPATGFAIGLDRTA
LALMKEGRRFVFRPTVAVLPLAEEARKVALEVQRELASLDVNSFLLTEQKSIKKALSFASSMNAKVAIIIGKKEVEENKV
TIKNLEKGLQVSCGRAELLDCLSETLKTP

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=450, Percent_Identity=25.1111111111111, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI15029520, Length=466, Percent_Identity=26.3948497854077, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788861, Length=456, Percent_Identity=27.1929824561404, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71993693, Length=468, Percent_Identity=26.7094017094017, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=469, Percent_Identity=26.0127931769723, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=421, Percent_Identity=26.3657957244656, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24643061, Length=349, Percent_Identity=26.3610315186246, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24643059, Length=310, Percent_Identity=27.741935483871, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48652; Mature: 48652

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDPEPLRGFRDLFGEEAEALEAVTEEAEKLARNWSFERCYLPTVERFELFAMKSGVEI
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCEE
ERSMYVFVDKGGRKVTLRPEATASAVRAYLRLLRPRPKPVKLFTVVNVFRYDEPQFARYR
ECEEEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCHHHHHH
EFYQADFEVFGASSPYQDSELLTMLHEYYENLGLPHEILLGSVKALRGVLSEEGIEERDQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCH
DVVLHYVDKGMIDKALELVENKARRPERAKEAVAVLSTKEGGEEVIKEGKELMESWGYKY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCE
RFEDLEEVLKYLPDDVKSVIRLKLGFARGLAYYTGLIYEVRVPGFPVSVAGGGRYDALTE
EHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEECCCCCHHHHHH
VYGWERVPATGFAIGLDRTALALMKEGRRFVFRPTVAVLPLAEEARKVALEVQRELASLD
HHCCCCCCCCCEEECCHHHHHHHHHCCCEEEEECCHHEECCHHHHHHHHHHHHHHHHHCC
VNSFLLTEQKSIKKALSFASSMNAKVAIIIGKKEVEENKVTIKNLEKGLQVSCGRAELLD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCHHHHHH
CLSETLKTP
HHHHHHCCC
>Mature Secondary Structure
MKLDPEPLRGFRDLFGEEAEALEAVTEEAEKLARNWSFERCYLPTVERFELFAMKSGVEI
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCEE
ERSMYVFVDKGGRKVTLRPEATASAVRAYLRLLRPRPKPVKLFTVVNVFRYDEPQFARYR
ECEEEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCHHHHHH
EFYQADFEVFGASSPYQDSELLTMLHEYYENLGLPHEILLGSVKALRGVLSEEGIEERDQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCH
DVVLHYVDKGMIDKALELVENKARRPERAKEAVAVLSTKEGGEEVIKEGKELMESWGYKY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCE
RFEDLEEVLKYLPDDVKSVIRLKLGFARGLAYYTGLIYEVRVPGFPVSVAGGGRYDALTE
EHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEECCCCCHHHHHH
VYGWERVPATGFAIGLDRTALALMKEGRRFVFRPTVAVLPLAEEARKVALEVQRELASLD
HHCCCCCCCCCEEECCHHHHHHHHHCCCEEEEECCHHEECCHHHHHHHHHHHHHHHHHCC
VNSFLLTEQKSIKKALSFASSMNAKVAIIIGKKEVEENKVTIKNLEKGLQVSCGRAELLD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCHHHHHH
CLSETLKTP
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]