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The map label for this gene is purF [H]

Identifier: 156937393

GI number: 156937393

Start: 536474

End: 537748

Strand: Reverse

Name: purF [H]

Synonym: Igni_0600

Alternate gene names: 156937393

Gene position: 537748-536474 (Counterclockwise)

Preceding gene: 156937394

Following gene: 156937391

Centisome position: 41.44

GC content: 58.35

Gene sequence:

>1275_bases
TTGGCCGGAATAGCGGGCGCTCTGGCCTTCGACCCGGTATGGAACGTAGCTAGGTTCGTGTACTACTCCTTGCTCTCGCT
CCAACACAGGGGACAAGAGGAGGCGTGCCTCTACGTCGCCGACGGTGAAAAGATAGAGTGGGTCTGTGGCAAGGGCTTCG
TCGACGAGGTCTTGCCTCCAGCCACCAACTTGGCCGGCTGGGCGGCCATAGGAGGGGTGTGGAGCGAACACCCGGGTTAC
CGGGCCTACTCAGAAGAGCCCGCGGAGGCGGCTGTAGTCTTGGACGGCAGGCTGTTCAACGCCACTCCCTACGACGTCGC
CAAGAAGCTCTCGGAGCTGAAGGCCAGCGGCTACGAGGGGGAGGACCTACTCAAAGAGCTCGTGAAGGACTTAAAGGGAG
CCTTTAGCATGCTCGCCTTAACTGCCTCCGGCGAGCTCTTGGCCTACCGCTCCCCTCCGGGGCTGAGGCCCCTCCAGCTG
GGAGGCTTGGGCTTCGACATGGTCACCTTCGCCTCCGAGAGCTGTGCGTTCGACGTCATAGGCGCCGACTTGAAGAAGGA
CCTCGACCCCGGGACGGGCTTCTACGCCTCTCAGCTGTACTTCAACGAGGTTTCGGTGAAGCCCAGCGAGAAGGTGGTCT
GTGCCTTCGAGTACATCTACAACGCGAGGCCGGACTCGGTCATAGACGGCGTAGAGGTCTACGAAGTGAGGGAGAGGATA
GGCAAGAGGCTCGCCCAGCTCTACCCGAGGGACGTGGACGTGGTCGTGGGCGTCCCGGAGACTGCGATACCATTTGCGAT
AGGGTACTCTAAGGAGGCCAAGAAAGACTACCGCTTGGGCTTTATCGCTACCGGGAGGAAGGCGAGGACTGCCATAAAGC
CAGACTTGAGCGAGAGGTTGATAGGAGTACAATTGAAGCTGAACCCGATAAGGAGCACCTTCAGAAAGAAGAGGGCCCTT
ATTATTGATGATAGCGTGGTCAGAGGCCTGACCTTGAAGACGGTGATACAAACCCTAAAGAGCAAGGTGGGAGCTGTGAG
CGTCGACGTACTCATAGGGAGCCCGAAGATAATAAGCCATTGCCCTTACGGCGTGGAGGTGCCCCCCGCGGACCAGCTGA
TCGCCGCTAAGATGGACAACGAAAAGATAGCCGAGTATATAGGGGCTAAGAGCATCGAGTGGCTCCCGCCGGAGGAGTTA
GAAAAACTAATACCGATCAAGGCTTGTATGGGCTGCTTTACCGGACGTTACCCGTTGAGGGAGGAACGATCTTAA

Upstream 100 bases:

>100_bases
AGGTGGGATAAGCTATTGCAGCGAGCTGAACGCCTACAAGGCCTTGTTCGGCGGGGTCGAATTTAACCTAAGGGGAGGAC
CGCGACAGCGGTAGTGAAGG

Downstream 100 bases:

>100_bases
CCAGCCTCATCTCAGTGGTCATGGTCCCTAGGGCCCTCTTCCCGTGCAAGCTCGCCATTTTCAACACGCTGTGACTTTCA
ACCATAACCATGTACTTCAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAGIAGALAFDPVWNVARFVYYSLLSLQHRGQEEACLYVADGEKIEWVCGKGFVDEVLPPATNLAGWAAIGGVWSEHPGY
RAYSEEPAEAAVVLDGRLFNATPYDVAKKLSELKASGYEGEDLLKELVKDLKGAFSMLALTASGELLAYRSPPGLRPLQL
GGLGFDMVTFASESCAFDVIGADLKKDLDPGTGFYASQLYFNEVSVKPSEKVVCAFEYIYNARPDSVIDGVEVYEVRERI
GKRLAQLYPRDVDVVVGVPETAIPFAIGYSKEAKKDYRLGFIATGRKARTAIKPDLSERLIGVQLKLNPIRSTFRKKRAL
IIDDSVVRGLTLKTVIQTLKSKVGAVSVDVLIGSPKIISHCPYGVEVPPADQLIAAKMDNEKIAEYIGAKSIEWLPPEEL
EKLIPIKACMGCFTGRYPLREERS

Sequences:

>Translated_424_residues
MAGIAGALAFDPVWNVARFVYYSLLSLQHRGQEEACLYVADGEKIEWVCGKGFVDEVLPPATNLAGWAAIGGVWSEHPGY
RAYSEEPAEAAVVLDGRLFNATPYDVAKKLSELKASGYEGEDLLKELVKDLKGAFSMLALTASGELLAYRSPPGLRPLQL
GGLGFDMVTFASESCAFDVIGADLKKDLDPGTGFYASQLYFNEVSVKPSEKVVCAFEYIYNARPDSVIDGVEVYEVRERI
GKRLAQLYPRDVDVVVGVPETAIPFAIGYSKEAKKDYRLGFIATGRKARTAIKPDLSERLIGVQLKLNPIRSTFRKKRAL
IIDDSVVRGLTLKTVIQTLKSKVGAVSVDVLIGSPKIISHCPYGVEVPPADQLIAAKMDNEKIAEYIGAKSIEWLPPEEL
EKLIPIKACMGCFTGRYPLREERS
>Mature_423_residues
AGIAGALAFDPVWNVARFVYYSLLSLQHRGQEEACLYVADGEKIEWVCGKGFVDEVLPPATNLAGWAAIGGVWSEHPGYR
AYSEEPAEAAVVLDGRLFNATPYDVAKKLSELKASGYEGEDLLKELVKDLKGAFSMLALTASGELLAYRSPPGLRPLQLG
GLGFDMVTFASESCAFDVIGADLKKDLDPGTGFYASQLYFNEVSVKPSEKVVCAFEYIYNARPDSVIDGVEVYEVRERIG
KRLAQLYPRDVDVVVGVPETAIPFAIGYSKEAKKDYRLGFIATGRKARTAIKPDLSERLIGVQLKLNPIRSTFRKKRALI
IDDSVVRGLTLKTVIQTLKSKVGAVSVDVLIGSPKIISHCPYGVEVPPADQLIAAKMDNEKIAEYIGAKSIEWLPPEELE
KLIPIKACMGCFTGRYPLREERS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=492, Percent_Identity=28.4552845528455, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI1788651, Length=456, Percent_Identity=31.359649122807, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=28.1573498964803, Blast_Score=148, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6323958, Length=479, Percent_Identity=30.8977035490605, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28573187, Length=485, Percent_Identity=28.0412371134021, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24659598, Length=467, Percent_Identity=27.8372591006424, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24659604, Length=308, Percent_Identity=32.4675324675325, Blast_Score=151, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 46452; Mature: 46321

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGIAGALAFDPVWNVARFVYYSLLSLQHRGQEEACLYVADGEKIEWVCGKGFVDEVLPP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHCCC
ATNLAGWAAIGGVWSEHPGYRAYSEEPAEAAVVLDGRLFNATPYDVAKKLSELKASGYEG
CCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCEEECCCHHHHHHHHHHHHHCCCCH
EDLLKELVKDLKGAFSMLALTASGELLAYRSPPGLRPLQLGGLGFDMVTFASESCAFDVI
HHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCEEEECCCCHHHHHHCCCCCEEEHH
GADLKKDLDPGTGFYASQLYFNEVSVKPSEKVVCAFEYIYNARPDSVIDGVEVYEVRERI
CCHHHHCCCCCCCCEEHHEEEEEECCCCCCCEEEEEHHHHCCCCCHHHCCHHHHHHHHHH
GKRLAQLYPRDVDVVVGVPETAIPFAIGYSKEAKKDYRLGFIATGRKARTAIKPDLSERL
HHHHHHHCCCCCEEEEECCCCCCCEEECCCHHCCCCCEEEEEECCCHHCCCCCCCHHHCE
IGVQLKLNPIRSTFRKKRALIIDDSVVRGLTLKTVIQTLKSKVGAVSVDVLIGSPKIISH
EEEEEEEHHHHHHHHHCCEEEEECHHHHCHHHHHHHHHHHHHCCEEEEEEEECCCHHHHC
CPYGVEVPPADQLIAAKMDNEKIAEYIGAKSIEWLPPEELEKLIPIKACMGCFTGRYPLR
CCCCCCCCCCHHHEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
EERS
CCCC
>Mature Secondary Structure 
AGIAGALAFDPVWNVARFVYYSLLSLQHRGQEEACLYVADGEKIEWVCGKGFVDEVLPP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHCCC
ATNLAGWAAIGGVWSEHPGYRAYSEEPAEAAVVLDGRLFNATPYDVAKKLSELKASGYEG
CCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCEEECCCHHHHHHHHHHHHHCCCCH
EDLLKELVKDLKGAFSMLALTASGELLAYRSPPGLRPLQLGGLGFDMVTFASESCAFDVI
HHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCEEEECCCCHHHHHHCCCCCEEEHH
GADLKKDLDPGTGFYASQLYFNEVSVKPSEKVVCAFEYIYNARPDSVIDGVEVYEVRERI
CCHHHHCCCCCCCCEEHHEEEEEECCCCCCCEEEEEHHHHCCCCCHHHCCHHHHHHHHHH
GKRLAQLYPRDVDVVVGVPETAIPFAIGYSKEAKKDYRLGFIATGRKARTAIKPDLSERL
HHHHHHHCCCCCEEEEECCCCCCCEEECCCHHCCCCCEEEEEECCCHHCCCCCCCHHHCE
IGVQLKLNPIRSTFRKKRALIIDDSVVRGLTLKTVIQTLKSKVGAVSVDVLIGSPKIISH
EEEEEEEHHHHHHHHHCCEEEEECHHHHCHHHHHHHHHHHHHCCEEEEEEEECCCHHHHC
CPYGVEVPPADQLIAAKMDNEKIAEYIGAKSIEWLPPEELEKLIPIKACMGCFTGRYPLR
CCCCCCCCCCHHHEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
EERS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]