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The map label for this gene is asnB [H]

Identifier: 156937363

GI number: 156937363

Start: 514046

End: 515878

Strand: Reverse

Name: asnB [H]

Synonym: Igni_0570

Alternate gene names: 156937363

Gene position: 515878-514046 (Counterclockwise)

Preceding gene: 156937364

Following gene: 156937362

Centisome position: 39.76

GC content: 52.59

Gene sequence:

>1833_bases
TTGTGCGGGATCGTAGCGGTGAGCTGCGCTAGGCCGTGCGTGGAGGAGCGCCGACTCGTCTCTGCTCTGGACGCAATAGA
ACACAGGGGGCCGGACGGGAGGGGGTATTGGATATCTGAGGACCGCAAGACCGCGCTGGGCAACGTCAGGCTAGCCATAA
TAGACCCTAAGGACGAGGGCCTCCAACCCATGTTCAACGAGGACGGTTCCGTGGGAGTAGTGTTGAACGGTGAGATATAC
AATTATAAAGAAATCCGCCAGAACTTGAAGGACGAACACGAGTTCTTGTCCAACACGGACACCGAGGTCTTGTTGCACGC
GTACGAGGAGATGGGAGTGGACGTGGTTCACTCCTTAAGGGGGATGTTCGCGTTCGCGATATACGACGAAAGGAAGGGCA
CAATATTCGTTGCCAGGGACCACGTGGGCAAGAAGCCTTTATTCTATTACCAAGGTGAGGACTTCGTCGTTCTCGCCTCA
GAGCCTTCAGCGATTAAGGCGTTCGTGAAGAACTTGAGCAAGAACCCCTTGGGAGCCTTCTACCTCTTCGCGCACTTCTC
CCCCCTCCCGGAACAGCACTTCTTCGAAAACGTCAAGTTGCTTAGGAACGGAGAATATATGATAATAAAAGACGGGAAGG
TTATAGAAAAGAAGAAGTACTACACCTTGAAGTTCGATAAAAGGGTGAGTGATATAACGAGCTACGTGAAGGAGCTCCAT
CGAGAGCTGATAAGGGCGGTGGAGTATAGGTTAATAGCGGACGTGCCGGTCTCCTTGGCCTTGAGCTCCGGCGTGGACTC
TACGTTGCTAGCCTCAATAATAACGAAGGAACTCAACAGGAACGACGTTAGCACCTTCACCGTGACTGTTAAGGGCGCCG
GGGAGTTCGACGAAGTGGAGTTGGCCTCTAAAGTTGCTGAGGAGCTAAACCTCGACCACGCAGTGGTCGAATTCGACTTT
GAGGATTACATAAAGCTCACTTACGAGCTGACCAAAAAGGTCGGGCTGCCCATAACTGACCCCAGCTTGGTGATGGGGTA
CGGGATGGCTAAGGCGGCGAGGGAAAGGGGCTACAAGGTAATCATCGTCGGGGAGGGAGGGGACGAGCTCTTCTTCGGCT
ACCCCGTGTATAAACAAATATACGAGCTCACCTTAACCCTCTCCTCCCTCCCCTTGCTCGAGCTCCCCTTCTGGGTCGTG
AAGGAAGTTAGAAGTTACTACAACAAGGCCTTGTGTTACGCCACCCTACACAAGCTCGTGACCGCCAGGGGGCTCTCCTT
GAGGTCCCAGTGCTGCGAGGCCTTCCCCCCGGAGCTCTGCGACCTCTTAGTGTTAAGCTACCGGGTTCTGGAGTCCCTCT
CCGCCCCTAGGAACCTCGCCGAGAGGCTCCTCTCGTACGAGTACAACCAGAGGATACCCAACCACTTGAACTTGAAGGTA
GACTTCTACTCTATGGCTGCGAGCGTGGAAGCCAGGGCGCCGCTATTGGACTACAAGTTAATAGAAAAAGCTATGAGCTT
AGACCCAGCTTGGAAGAGATACATAATGATGAAAGAGCCAAAGCTCATTGGGAAGTTGATATTAAAGAAATACTTGAGTG
AGGAGACTTGGAGAAAGGTCACATCTAGGAAGGTCGGCTTCGGACAGCAAGCTAAGGACGTCCTAATGAGGATAGCCGAG
GAGGAGGCACCCTCAAGGCTCAAGAGCAGCGAGATATTAAAAAAGGTCATAGACCCGAACATATTTGAGAGGTACGATAC
GAAGAGGTTGTCATTCGTAGCGTGGCAAGTGTTCTCCTACAGCGTTTGGGAGGAGGTGATCGCTACTTGGTAA

Upstream 100 bases:

>100_bases
CGGAATTGGTGGAGAGGAGGTGGCGCGAGTACGGCTTTTAGGTCAAAAGTCGCGCGTCGCGGGCCCTTAGCCGGAGCGTG
CCGGCGAAAGGTGAAGGGGC

Downstream 100 bases:

>100_bases
AAGTCAGCGTGGTTTACGGCCACCCGGAGGTCCGGGTAGCGGGCGGTTCTTATTCTCATGTAGTTAACTTAGCAACCTCA
ATAGCTCAAGTTTTGAAGGA

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MCGIVAVSCARPCVEERRLVSALDAIEHRGPDGRGYWISEDRKTALGNVRLAIIDPKDEGLQPMFNEDGSVGVVLNGEIY
NYKEIRQNLKDEHEFLSNTDTEVLLHAYEEMGVDVVHSLRGMFAFAIYDERKGTIFVARDHVGKKPLFYYQGEDFVVLAS
EPSAIKAFVKNLSKNPLGAFYLFAHFSPLPEQHFFENVKLLRNGEYMIIKDGKVIEKKKYYTLKFDKRVSDITSYVKELH
RELIRAVEYRLIADVPVSLALSSGVDSTLLASIITKELNRNDVSTFTVTVKGAGEFDEVELASKVAEELNLDHAVVEFDF
EDYIKLTYELTKKVGLPITDPSLVMGYGMAKAARERGYKVIIVGEGGDELFFGYPVYKQIYELTLTLSSLPLLELPFWVV
KEVRSYYNKALCYATLHKLVTARGLSLRSQCCEAFPPELCDLLVLSYRVLESLSAPRNLAERLLSYEYNQRIPNHLNLKV
DFYSMAASVEARAPLLDYKLIEKAMSLDPAWKRYIMMKEPKLIGKLILKKYLSEETWRKVTSRKVGFGQQAKDVLMRIAE
EEAPSRLKSSEILKKVIDPNIFERYDTKRLSFVAWQVFSYSVWEEVIATW

Sequences:

>Translated_610_residues
MCGIVAVSCARPCVEERRLVSALDAIEHRGPDGRGYWISEDRKTALGNVRLAIIDPKDEGLQPMFNEDGSVGVVLNGEIY
NYKEIRQNLKDEHEFLSNTDTEVLLHAYEEMGVDVVHSLRGMFAFAIYDERKGTIFVARDHVGKKPLFYYQGEDFVVLAS
EPSAIKAFVKNLSKNPLGAFYLFAHFSPLPEQHFFENVKLLRNGEYMIIKDGKVIEKKKYYTLKFDKRVSDITSYVKELH
RELIRAVEYRLIADVPVSLALSSGVDSTLLASIITKELNRNDVSTFTVTVKGAGEFDEVELASKVAEELNLDHAVVEFDF
EDYIKLTYELTKKVGLPITDPSLVMGYGMAKAARERGYKVIIVGEGGDELFFGYPVYKQIYELTLTLSSLPLLELPFWVV
KEVRSYYNKALCYATLHKLVTARGLSLRSQCCEAFPPELCDLLVLSYRVLESLSAPRNLAERLLSYEYNQRIPNHLNLKV
DFYSMAASVEARAPLLDYKLIEKAMSLDPAWKRYIMMKEPKLIGKLILKKYLSEETWRKVTSRKVGFGQQAKDVLMRIAE
EEAPSRLKSSEILKKVIDPNIFERYDTKRLSFVAWQVFSYSVWEEVIATW
>Mature_610_residues
MCGIVAVSCARPCVEERRLVSALDAIEHRGPDGRGYWISEDRKTALGNVRLAIIDPKDEGLQPMFNEDGSVGVVLNGEIY
NYKEIRQNLKDEHEFLSNTDTEVLLHAYEEMGVDVVHSLRGMFAFAIYDERKGTIFVARDHVGKKPLFYYQGEDFVVLAS
EPSAIKAFVKNLSKNPLGAFYLFAHFSPLPEQHFFENVKLLRNGEYMIIKDGKVIEKKKYYTLKFDKRVSDITSYVKELH
RELIRAVEYRLIADVPVSLALSSGVDSTLLASIITKELNRNDVSTFTVTVKGAGEFDEVELASKVAEELNLDHAVVEFDF
EDYIKLTYELTKKVGLPITDPSLVMGYGMAKAARERGYKVIIVGEGGDELFFGYPVYKQIYELTLTLSSLPLLELPFWVV
KEVRSYYNKALCYATLHKLVTARGLSLRSQCCEAFPPELCDLLVLSYRVLESLSAPRNLAERLLSYEYNQRIPNHLNLKV
DFYSMAASVEARAPLLDYKLIEKAMSLDPAWKRYIMMKEPKLIGKLILKKYLSEETWRKVTSRKVGFGQQAKDVLMRIAE
EEAPSRLKSSEILKKVIDPNIFERYDTKRLSFVAWQVFSYSVWEEVIATW

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=404, Percent_Identity=29.4554455445545, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI168229252, Length=404, Percent_Identity=29.4554455445545, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI168229250, Length=404, Percent_Identity=29.4554455445545, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI296010848, Length=379, Percent_Identity=29.023746701847, Blast_Score=105, Evalue=2e-22,
Organism=Escherichia coli, GI1786889, Length=417, Percent_Identity=28.0575539568345, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25147557, Length=395, Percent_Identity=27.8481012658228, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71993933, Length=395, Percent_Identity=27.5949367088608, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560178, Length=393, Percent_Identity=28.2442748091603, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25147560, Length=332, Percent_Identity=27.710843373494, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325403, Length=397, Percent_Identity=26.9521410579345, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321563, Length=399, Percent_Identity=25.8145363408521, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45553209, Length=424, Percent_Identity=30.4245283018868, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69718; Mature: 69718

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVAVSCARPCVEERRLVSALDAIEHRGPDGRGYWISEDRKTALGNVRLAIIDPKDEG
CCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHCCCEEEEEECCCCCC
LQPMFNEDGSVGVVLNGEIYNYKEIRQNLKDEHEFLSNTDTEVLLHAYEEMGVDVVHSLR
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHH
GMFAFAIYDERKGTIFVARDHVGKKPLFYYQGEDFVVLASEPSAIKAFVKNLSKNPLGAF
HHEEEEEEECCCCEEEEEECCCCCCCEEEECCCCEEEEECCCHHHHHHHHHCCCCCCHHE
YLFAHFSPLPEQHFFENVKLLRNGEYMIIKDGKVIEKKKYYTLKFDKRVSDITSYVKELH
EHHHHCCCCCHHHHHHHHHHEECCCEEEEECCCEEECCCEEEEEEHHHHHHHHHHHHHHH
RELIRAVEYRLIADVPVSLALSSGVDSTLLASIITKELNRNDVSTFTVTVKGAGEFDEVE
HHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHH
LASKVAEELNLDHAVVEFDFEDYIKLTYELTKKVGLPITDPSLVMGYGMAKAARERGYKV
HHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHCCCEE
IIVGEGGDELFFGYPVYKQIYELTLTLSSLPLLELPFWVVKEVRSYYNKALCYATLHKLV
EEEECCCCCEEECCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TARGLSLRSQCCEAFPPELCDLLVLSYRVLESLSAPRNLAERLLSYEYNQRIPNHLNLKV
HHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEE
DFYSMAASVEARAPLLDYKLIEKAMSLDPAWKRYIMMKEPKLIGKLILKKYLSEETWRKV
EHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHEEECCHHHHHHHHHHHHHCHHHHHHH
TSRKVGFGQQAKDVLMRIAEEEAPSRLKSSEILKKVIDPNIFERYDTKRLSFVAWQVFSY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHH
SVWEEVIATW
HHHHHHHHCC
>Mature Secondary Structure
MCGIVAVSCARPCVEERRLVSALDAIEHRGPDGRGYWISEDRKTALGNVRLAIIDPKDEG
CCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHCCCEEEEEECCCCCC
LQPMFNEDGSVGVVLNGEIYNYKEIRQNLKDEHEFLSNTDTEVLLHAYEEMGVDVVHSLR
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHH
GMFAFAIYDERKGTIFVARDHVGKKPLFYYQGEDFVVLASEPSAIKAFVKNLSKNPLGAF
HHEEEEEEECCCCEEEEEECCCCCCCEEEECCCCEEEEECCCHHHHHHHHHCCCCCCHHE
YLFAHFSPLPEQHFFENVKLLRNGEYMIIKDGKVIEKKKYYTLKFDKRVSDITSYVKELH
EHHHHCCCCCHHHHHHHHHHEECCCEEEEECCCEEECCCEEEEEEHHHHHHHHHHHHHHH
RELIRAVEYRLIADVPVSLALSSGVDSTLLASIITKELNRNDVSTFTVTVKGAGEFDEVE
HHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHH
LASKVAEELNLDHAVVEFDFEDYIKLTYELTKKVGLPITDPSLVMGYGMAKAARERGYKV
HHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHCCCEE
IIVGEGGDELFFGYPVYKQIYELTLTLSSLPLLELPFWVVKEVRSYYNKALCYATLHKLV
EEEECCCCCEEECCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TARGLSLRSQCCEAFPPELCDLLVLSYRVLESLSAPRNLAERLLSYEYNQRIPNHLNLKV
HHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEE
DFYSMAASVEARAPLLDYKLIEKAMSLDPAWKRYIMMKEPKLIGKLILKKYLSEETWRKV
EHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHEEECCHHHHHHHHHHHHHCHHHHHHH
TSRKVGFGQQAKDVLMRIAEEEAPSRLKSSEILKKVIDPNIFERYDTKRLSFVAWQVFSY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHH
SVWEEVIATW
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]