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The map label for this gene is nuoM [H]

Identifier: 156937338

GI number: 156937338

Start: 488854

End: 490344

Strand: Reverse

Name: nuoM [H]

Synonym: Igni_0545

Alternate gene names: 156937338

Gene position: 490344-488854 (Counterclockwise)

Preceding gene: 156937339

Following gene: 156937337

Centisome position: 37.79

GC content: 57.41

Gene sequence:

>1491_bases
GTGTGTGAGGTGTTAGTATCCATAATAATAGCGGCCTTAGCGTCGGTAGGAGTGTTGCTAATAGGTGAAAGGAAGGCCAA
GGCTTTTGCGGCCGTCATAACGGTAATTATCGCCTTACTGCTGGCGAAGCCCATAGTAGTCGGGGCCTCCGGGGGGATCT
ATTCGGAGAGCTACGTCATACCGGGCCTCCACGCCATAGGGGCTGACTTGACCTTCAGAGTGGACGCGCTCAGCTCGCTT
TTCTTAGCGATAACCGCGCTCATCTTCATCGCCGTAGCTTGGGCGGCCAGCTGGGAGATTGAGGAAAGGCCCAGGCTGTA
CTTGGCACTAATGCTGGCCTCAGAGGCAGCCCTCATAGGGGTCTTCGCGACGTGGAACCTCTTCTGGTTCTACCTCTTCT
GGGAGTTCACGCTGTTGCCAATGTTCATCCACATACTTTACTGGGGCGGCGAGAGGAAGGTGTACGCGGCCTACAAGTTC
TTCGTGTACACCCAAGTGGGAGGGTTAATGTTGTTGGTTGCCATAGCGCTGGGCTGGCTCTACGGGGCCAACACCTTCGA
CTTCACGGAGTTCGCCGCCTCTTTGGCGGTGGCGCCGGACTGGGTTAAGGTAGCCTTCGCTTGGCTAACCTTGCTGTCGT
TCTTCATCAAGATGCCGGTCCCGCCGTTCCACACGTGGCTCCCCGCCGCCCACGTGGAGGCGCCCAGTCCGAGCTCCGTC
ATACTGGCGTCGCTGATGCTAAAGATGGGCGGCTACGCGTTCATAAGGATAACCTTGGGAATGGCACCCTACGTCCTAAT
GGGTGCCCTACCCTTCATAGCGGTCTGGGCGGCTCTGGCGGCGGTATACGCCTCCACCGTGGCCATAGCTCAAGAGGACT
TCAAGAGGGTAGTTGCATATACCAGCATCACTCACATGAGCATGAGCACCATAGGGGCCGCCGTATGGGCCTTGGGCCTC
AAGGAAGCTTCGTTGCTAGGCTTCATAGGTTCAGTATTCGAAATGATATCCCACAGCTTCGTGGTAGGGGCGCTGTTCTT
GCTCAGCGGGGTCATAAAGCACTACTGCCACACTAGGAAGATACCCGCCATAAGGGGGCTGGCCTTCGTGGCGCCGATAC
TCAGCACCGTAATAATGGTTGCTATCTTCGCCGGCTTCGGGCTGCCGGGCACCAGCGGCTACCCGGCGGAGCTCAGCTTG
GTGGCCTCGGCAGCTGGCATGGCAAAGTACAACCCCGCAGGCTCCTTGGTGTTGGCACTACTGTTAATAGCCATAGCGAC
CGTCACGGGCTACTTGATATGGGACGCTCAGAGGATGCTCTTCACCCGCCCGCCTCTGGAGGTAAAGGACGTTCGGTTCT
ACCACATAGGCCCCATACTGTACTTGCTCGCGATAAGCGTCATAATAGGCGTCGTGCCAGCCCTCGTAATGGACCCGCTA
ATTAAGGCTGTTCACCAGATGCCAGTACTGCTCGCGGGGGTGATAGGATGA

Upstream 100 bases:

>100_bases
GAACGGCGATATAAACCTCTACGTAGCGCTCTCAGCGGCAGGGCTGACGTTATTGATGTTTGTAACGCTGATATTGTTGT
TCGGTGTGGGGTGGTAAGCC

Downstream 100 bases:

>100_bases
TCGAGCCCGGCGCCGTCGCCTTCACCGTAGTGGCTGTCGCCATGGTCGTCGGCGGCCTCGTAGAGGGCGCCTCGCTTCTC
ATAAACAAGCTTCTGGAGGA

Product: proton-translocating NADH-quinone oxidoreductase, chain M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MCEVLVSIIIAALASVGVLLIGERKAKAFAAVITVIIALLLAKPIVVGASGGIYSESYVIPGLHAIGADLTFRVDALSSL
FLAITALIFIAVAWAASWEIEERPRLYLALMLASEAALIGVFATWNLFWFYLFWEFTLLPMFIHILYWGGERKVYAAYKF
FVYTQVGGLMLLVAIALGWLYGANTFDFTEFAASLAVAPDWVKVAFAWLTLLSFFIKMPVPPFHTWLPAAHVEAPSPSSV
ILASLMLKMGGYAFIRITLGMAPYVLMGALPFIAVWAALAAVYASTVAIAQEDFKRVVAYTSITHMSMSTIGAAVWALGL
KEASLLGFIGSVFEMISHSFVVGALFLLSGVIKHYCHTRKIPAIRGLAFVAPILSTVIMVAIFAGFGLPGTSGYPAELSL
VASAAGMAKYNPAGSLVLALLLIAIATVTGYLIWDAQRMLFTRPPLEVKDVRFYHIGPILYLLAISVIIGVVPALVMDPL
IKAVHQMPVLLAGVIG

Sequences:

>Translated_496_residues
MCEVLVSIIIAALASVGVLLIGERKAKAFAAVITVIIALLLAKPIVVGASGGIYSESYVIPGLHAIGADLTFRVDALSSL
FLAITALIFIAVAWAASWEIEERPRLYLALMLASEAALIGVFATWNLFWFYLFWEFTLLPMFIHILYWGGERKVYAAYKF
FVYTQVGGLMLLVAIALGWLYGANTFDFTEFAASLAVAPDWVKVAFAWLTLLSFFIKMPVPPFHTWLPAAHVEAPSPSSV
ILASLMLKMGGYAFIRITLGMAPYVLMGALPFIAVWAALAAVYASTVAIAQEDFKRVVAYTSITHMSMSTIGAAVWALGL
KEASLLGFIGSVFEMISHSFVVGALFLLSGVIKHYCHTRKIPAIRGLAFVAPILSTVIMVAIFAGFGLPGTSGYPAELSL
VASAAGMAKYNPAGSLVLALLLIAIATVTGYLIWDAQRMLFTRPPLEVKDVRFYHIGPILYLLAISVIIGVVPALVMDPL
IKAVHQMPVLLAGVIG
>Mature_496_residues
MCEVLVSIIIAALASVGVLLIGERKAKAFAAVITVIIALLLAKPIVVGASGGIYSESYVIPGLHAIGADLTFRVDALSSL
FLAITALIFIAVAWAASWEIEERPRLYLALMLASEAALIGVFATWNLFWFYLFWEFTLLPMFIHILYWGGERKVYAAYKF
FVYTQVGGLMLLVAIALGWLYGANTFDFTEFAASLAVAPDWVKVAFAWLTLLSFFIKMPVPPFHTWLPAAHVEAPSPSSV
ILASLMLKMGGYAFIRITLGMAPYVLMGALPFIAVWAALAAVYASTVAIAQEDFKRVVAYTSITHMSMSTIGAAVWALGL
KEASLLGFIGSVFEMISHSFVVGALFLLSGVIKHYCHTRKIPAIRGLAFVAPILSTVIMVAIFAGFGLPGTSGYPAELSL
VASAAGMAKYNPAGSLVLALLLIAIATVTGYLIWDAQRMLFTRPPLEVKDVRFYHIGPILYLLAISVIIGVVPALVMDPL
IKAVHQMPVLLAGVIG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=381, Percent_Identity=30.4461942257218, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI251831117, Length=375, Percent_Identity=24.2666666666667, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788613, Length=365, Percent_Identity=32.8767123287671, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788827, Length=350, Percent_Identity=24, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788829, Length=369, Percent_Identity=26.8292682926829, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788831, Length=456, Percent_Identity=24.5614035087719, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788614, Length=453, Percent_Identity=28.9183222958057, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53432; Mature: 53432

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCEVLVSIIIAALASVGVLLIGERKAKAFAAVITVIIALLLAKPIVVGASGGIYSESYVI
CHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
PGLHAIGADLTFRVDALSSLFLAITALIFIAVAWAASWEIEERPRLYLALMLASEAALIG
CCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VFATWNLFWFYLFWEFTLLPMFIHILYWGGERKVYAAYKFFVYTQVGGLMLLVAIALGWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YGANTFDFTEFAASLAVAPDWVKVAFAWLTLLSFFIKMPVPPFHTWLPAAHVEAPSPSSV
HCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHH
ILASLMLKMGGYAFIRITLGMAPYVLMGALPFIAVWAALAAVYASTVAIAQEDFKRVVAY
HHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSITHMSMSTIGAAVWALGLKEASLLGFIGSVFEMISHSFVVGALFLLSGVIKHYCHTRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPAIRGLAFVAPILSTVIMVAIFAGFGLPGTSGYPAELSLVASAAGMAKYNPAGSLVLAL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
LLIAIATVTGYLIWDAQRMLFTRPPLEVKDVRFYHIGPILYLLAISVIIGVVPALVMDPL
HHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IKAVHQMPVLLAGVIG
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCEVLVSIIIAALASVGVLLIGERKAKAFAAVITVIIALLLAKPIVVGASGGIYSESYVI
CHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
PGLHAIGADLTFRVDALSSLFLAITALIFIAVAWAASWEIEERPRLYLALMLASEAALIG
CCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VFATWNLFWFYLFWEFTLLPMFIHILYWGGERKVYAAYKFFVYTQVGGLMLLVAIALGWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YGANTFDFTEFAASLAVAPDWVKVAFAWLTLLSFFIKMPVPPFHTWLPAAHVEAPSPSSV
HCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHH
ILASLMLKMGGYAFIRITLGMAPYVLMGALPFIAVWAALAAVYASTVAIAQEDFKRVVAY
HHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSITHMSMSTIGAAVWALGLKEASLLGFIGSVFEMISHSFVVGALFLLSGVIKHYCHTRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPAIRGLAFVAPILSTVIMVAIFAGFGLPGTSGYPAELSLVASAAGMAKYNPAGSLVLAL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
LLIAIATVTGYLIWDAQRMLFTRPPLEVKDVRFYHIGPILYLLAISVIIGVVPALVMDPL
HHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IKAVHQMPVLLAGVIG
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]