Click here to switch to the map view.

The map label for this gene is ndhF [H]

Identifier: 156937339

GI number: 156937339

Start: 490348

End: 492426

Strand: Reverse

Name: ndhF [H]

Synonym: Igni_0546

Alternate gene names: 156937339

Gene position: 492426-490348 (Counterclockwise)

Preceding gene: 156937340

Following gene: 156937338

Centisome position: 37.95

GC content: 58.2

Gene sequence:

>2079_bases
ATGGAAGGCGCGTCTCACCAAGTCCCGCCGAGCTTAGCGTTGGTCGCGCCAGTTCCCATAGCTTTGCCGTTAGTGATCTC
AATAATTGTGCTGCTCAGTCCATTGTACAAGAAGAAGATTAAGTTCAAGCCTTTGTGGGCCGGCGACAGGGAGGACTGGT
GGGAGTGGTCCTTAGCGGTCGCGACGGCGGGGGTAGTGGTCTTAGCTACCGCTTACTTGCTTGCGGAGGGCTACGGCAAG
AGCTACGAGATTAAGTACTACTCGTGGTACCCCCTGCCCTCACATCCCAACGACTTCTCCCTCCTCATTGACACTATTTC
AGGAATAATGGCAATAGTAGTAGCTACGATAACCTTCTTGGACTTGGTGTACAGCTGGGAATACATGGCCGGCGCCCGCG
GCCCCAACCGGTACTACAGCGAAATGATGCTGTTCTTGGCTTCGATGGAAGGTATAGTGCTCTCTGGAAACTTGGTCTTG
ATAATGTTGTTCTGGGAACTGGTGGGAGCTGCCAGCTTCCTGCTGATCTCCTACTACTGGTACGACCCAATTATAGGTCC
TAACGCGGTGAGGGCGGGGAGGAAGGCAATACTGGTCACCAGGGTGGCGGACTTATTCTTCTTGGCAGGCTTGGGCGCGC
TCATAGCCTTGGCGGGCACGGGTAACGTCTTGGAGCTTCAGCACTACGAGACCTTAGCGTTCCAGAAGTGGCTGGGGGCG
GCGGCGCTGACCGCAATACTGGTAGGGATAACCATAGGGGCCTTCGGCAAGAGCGCCCAAGTGCCCTTCTGGCCGTGGCT
CTCCGACGCCATGGAGGGACCCACGACCGTCTCCGCCGTGCTCCACTCCGCCACCATGGTGGCGGCCGGCGCTTACCTAA
TAGCCCGGCTCTTCCCCTTGTACGAGGAGTTCATAGCTTTCAACCTGGCACTGATGGACTTCATAGCGATAGTCGGAGCG
GTGACCGCGCTGGTCGCGGGCTTGTTCGGCGCGGCGGCGAGAGACATAAAGAAGGTGATAGCTTTCTCCACCATGAGCCA
GCTGGGCTACATGTTCGCCGCTCTGGGATTGGGGAGCTTGGTCGCCGGGGCGGCGCACTTGTACATACACGCCTTCTTCA
AGGCCCTCCTGTTCTTGGGCGCGGGCGCGGTCATACACTCCTTAGAGCACGTCTTGCATCACCCCTACAAGGCTAGGGAC
ATGTTCAACATGGGCGGGCTGTGGAGGTACATGAAAGTTACCTTCGTGGTGACGTTAATAGCGCTCTTGAGCTTGATAGG
CCTGCCGCCCTTCTCAGGCTGGTGGTCTAAGGAGCTAATAATAGAGAGCGCGCTCCACAGCCCGGTCCCCCACGCCTTAG
CTGTAGGGGTGACGCTCACCTTAGCGGCCGGCTTGACCGGCTTCTACAGCGGAAGGCTGCTGTACCTCACCTTCTTCGGC
AAGCCGCGCTGGAAGGCACACGGGGAGCACCTCCACGACGCCGGCCCCGCGATGAAGTTCGCATTGGTTACCCTAGCGCT
GATAGTCCTAGTCTCCGGCCCTACGATAACGTTCGCGCTGAAGAAGGCGCTCCGGGCCCACGAGGGCTTGCCTAGGTACG
AGCTCCCTCTGGGCAACGCGGCCCTAACCTTGGCCATAATAGGCTTCCTAACGCCCATCACCTACTACGTGCTAATGGGC
GTCGCCGAGAGGGGCGGGATAATAAGGAAGGTCTGGTACCCGCTCTACAAGGAGTTCTGGTTCCACGAGTTCTTCCACTC
CGTGGCCAACTTCTGGGCTTACGCGCTGTCGAAGGCGGTGGACGCCGTCGAGAGGGCTTATACAGCCTTCTTGGTCGGGA
TGGCCTTCTTCATAGGCCGCGCCTACGCGTGGGGCTGGGCTGTAAGCGTTAGGCTGGCGGACGAGGACTACTGGGACGTC
AAGGTGATCGACGGACTTGCGAGGTCTGTAGTCAAGCTGGGCAAGAAGGCTCTCGAGCTACAGAACGGCGATATAAACCT
CTACGTAGCGCTCTCAGCGGCAGGGCTGACGTTATTGATGTTTGTAACGCTGATATTGTTGTTCGGTGTGGGGTGGTAA

Upstream 100 bases:

>100_bases
ACGGGGACGAGGTAGAGGTCCTCGTTTACTAGGCCGCTACGAGGCCCGGAAGATGAGTTTTATCAACGCCTTTCTTCCCT
CTCAATTCGGTGTCTAAATA

Downstream 100 bases:

>100_bases
GCCGTGTGTGAGGTGTTAGTATCCATAATAATAGCGGCCTTAGCGTCGGTAGGAGTGTTGCTAATAGGTGAAAGGAAGGC
CAAGGCTTTTGCGGCCGTCA

Product: proton-translocating NADH-quinone oxidoreductase, chain L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MEGASHQVPPSLALVAPVPIALPLVISIIVLLSPLYKKKIKFKPLWAGDREDWWEWSLAVATAGVVVLATAYLLAEGYGK
SYEIKYYSWYPLPSHPNDFSLLIDTISGIMAIVVATITFLDLVYSWEYMAGARGPNRYYSEMMLFLASMEGIVLSGNLVL
IMLFWELVGAASFLLISYYWYDPIIGPNAVRAGRKAILVTRVADLFFLAGLGALIALAGTGNVLELQHYETLAFQKWLGA
AALTAILVGITIGAFGKSAQVPFWPWLSDAMEGPTTVSAVLHSATMVAAGAYLIARLFPLYEEFIAFNLALMDFIAIVGA
VTALVAGLFGAAARDIKKVIAFSTMSQLGYMFAALGLGSLVAGAAHLYIHAFFKALLFLGAGAVIHSLEHVLHHPYKARD
MFNMGGLWRYMKVTFVVTLIALLSLIGLPPFSGWWSKELIIESALHSPVPHALAVGVTLTLAAGLTGFYSGRLLYLTFFG
KPRWKAHGEHLHDAGPAMKFALVTLALIVLVSGPTITFALKKALRAHEGLPRYELPLGNAALTLAIIGFLTPITYYVLMG
VAERGGIIRKVWYPLYKEFWFHEFFHSVANFWAYALSKAVDAVERAYTAFLVGMAFFIGRAYAWGWAVSVRLADEDYWDV
KVIDGLARSVVKLGKKALELQNGDINLYVALSAAGLTLLMFVTLILLFGVGW

Sequences:

>Translated_692_residues
MEGASHQVPPSLALVAPVPIALPLVISIIVLLSPLYKKKIKFKPLWAGDREDWWEWSLAVATAGVVVLATAYLLAEGYGK
SYEIKYYSWYPLPSHPNDFSLLIDTISGIMAIVVATITFLDLVYSWEYMAGARGPNRYYSEMMLFLASMEGIVLSGNLVL
IMLFWELVGAASFLLISYYWYDPIIGPNAVRAGRKAILVTRVADLFFLAGLGALIALAGTGNVLELQHYETLAFQKWLGA
AALTAILVGITIGAFGKSAQVPFWPWLSDAMEGPTTVSAVLHSATMVAAGAYLIARLFPLYEEFIAFNLALMDFIAIVGA
VTALVAGLFGAAARDIKKVIAFSTMSQLGYMFAALGLGSLVAGAAHLYIHAFFKALLFLGAGAVIHSLEHVLHHPYKARD
MFNMGGLWRYMKVTFVVTLIALLSLIGLPPFSGWWSKELIIESALHSPVPHALAVGVTLTLAAGLTGFYSGRLLYLTFFG
KPRWKAHGEHLHDAGPAMKFALVTLALIVLVSGPTITFALKKALRAHEGLPRYELPLGNAALTLAIIGFLTPITYYVLMG
VAERGGIIRKVWYPLYKEFWFHEFFHSVANFWAYALSKAVDAVERAYTAFLVGMAFFIGRAYAWGWAVSVRLADEDYWDV
KVIDGLARSVVKLGKKALELQNGDINLYVALSAAGLTLLMFVTLILLFGVGW
>Mature_692_residues
MEGASHQVPPSLALVAPVPIALPLVISIIVLLSPLYKKKIKFKPLWAGDREDWWEWSLAVATAGVVVLATAYLLAEGYGK
SYEIKYYSWYPLPSHPNDFSLLIDTISGIMAIVVATITFLDLVYSWEYMAGARGPNRYYSEMMLFLASMEGIVLSGNLVL
IMLFWELVGAASFLLISYYWYDPIIGPNAVRAGRKAILVTRVADLFFLAGLGALIALAGTGNVLELQHYETLAFQKWLGA
AALTAILVGITIGAFGKSAQVPFWPWLSDAMEGPTTVSAVLHSATMVAAGAYLIARLFPLYEEFIAFNLALMDFIAIVGA
VTALVAGLFGAAARDIKKVIAFSTMSQLGYMFAALGLGSLVAGAAHLYIHAFFKALLFLGAGAVIHSLEHVLHHPYKARD
MFNMGGLWRYMKVTFVVTLIALLSLIGLPPFSGWWSKELIIESALHSPVPHALAVGVTLTLAAGLTGFYSGRLLYLTFFG
KPRWKAHGEHLHDAGPAMKFALVTLALIVLVSGPTITFALKKALRAHEGLPRYELPLGNAALTLAIIGFLTPITYYVLMG
VAERGGIIRKVWYPLYKEFWFHEFFHSVANFWAYALSKAVDAVERAYTAFLVGMAFFIGRAYAWGWAVSVRLADEDYWDV
KVIDGLARSVVKLGKKALELQNGDINLYVALSAAGLTLLMFVTLILLFGVGW

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=455, Percent_Identity=34.5054945054945, Blast_Score=199, Evalue=1e-50,
Organism=Escherichia coli, GI1788614, Length=456, Percent_Identity=37.9385964912281, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788829, Length=440, Percent_Identity=31.3636363636364, Blast_Score=153, Evalue=4e-38,
Organism=Escherichia coli, GI1788827, Length=441, Percent_Identity=25.3968253968254, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1788831, Length=360, Percent_Identity=27.2222222222222, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1788613, Length=417, Percent_Identity=23.9808153477218, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI2367154, Length=220, Percent_Identity=28.6363636363636, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 75689; Mature: 75689

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00024 HEMOPEXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGASHQVPPSLALVAPVPIALPLVISIIVLLSPLYKKKIKFKPLWAGDREDWWEWSLAV
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
ATAGVVVLATAYLLAEGYGKSYEIKYYSWYPLPSHPNDFSLLIDTISGIMAIVVATITFL
HHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DLVYSWEYMAGARGPNRYYSEMMLFLASMEGIVLSGNLVLIMLFWELVGAASFLLISYYW
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
YDPIIGPNAVRAGRKAILVTRVADLFFLAGLGALIALAGTGNVLELQHYETLAFQKWLGA
HCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHH
AALTAILVGITIGAFGKSAQVPFWPWLSDAMEGPTTVSAVLHSATMVAAGAYLIARLFPL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YEEFIAFNLALMDFIAIVGAVTALVAGLFGAAARDIKKVIAFSTMSQLGYMFAALGLGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGAAHLYIHAFFKALLFLGAGAVIHSLEHVLHHPYKARDMFNMGGLWRYMKVTFVVTLI
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHH
ALLSLIGLPPFSGWWSKELIIESALHSPVPHALAVGVTLTLAAGLTGFYSGRLLYLTFFG
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEC
KPRWKAHGEHLHDAGPAMKFALVTLALIVLVSGPTITFALKKALRAHEGLPRYELPLGNA
CCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCH
ALTLAIIGFLTPITYYVLMGVAERGGIIRKVWYPLYKEFWFHEFFHSVANFWAYALSKAV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAVERAYTAFLVGMAFFIGRAYAWGWAVSVRLADEDYWDVKVIDGLARSVVKLGKKALEL
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
QNGDINLYVALSAAGLTLLMFVTLILLFGVGW
CCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEGASHQVPPSLALVAPVPIALPLVISIIVLLSPLYKKKIKFKPLWAGDREDWWEWSLAV
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
ATAGVVVLATAYLLAEGYGKSYEIKYYSWYPLPSHPNDFSLLIDTISGIMAIVVATITFL
HHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DLVYSWEYMAGARGPNRYYSEMMLFLASMEGIVLSGNLVLIMLFWELVGAASFLLISYYW
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
YDPIIGPNAVRAGRKAILVTRVADLFFLAGLGALIALAGTGNVLELQHYETLAFQKWLGA
HCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHH
AALTAILVGITIGAFGKSAQVPFWPWLSDAMEGPTTVSAVLHSATMVAAGAYLIARLFPL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YEEFIAFNLALMDFIAIVGAVTALVAGLFGAAARDIKKVIAFSTMSQLGYMFAALGLGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGAAHLYIHAFFKALLFLGAGAVIHSLEHVLHHPYKARDMFNMGGLWRYMKVTFVVTLI
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHH
ALLSLIGLPPFSGWWSKELIIESALHSPVPHALAVGVTLTLAAGLTGFYSGRLLYLTFFG
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEC
KPRWKAHGEHLHDAGPAMKFALVTLALIVLVSGPTITFALKKALRAHEGLPRYELPLGNA
CCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCH
ALTLAIIGFLTPITYYVLMGVAERGGIIRKVWYPLYKEFWFHEFFHSVANFWAYALSKAV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAVERAYTAFLVGMAFFIGRAYAWGWAVSVRLADEDYWDVKVIDGLARSVVKLGKKALEL
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
QNGDINLYVALSAAGLTLLMFVTLILLFGVGW
CCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]