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The map label for this gene is pyrE [H]

Identifier: 156937289

GI number: 156937289

Start: 442365

End: 442931

Strand: Direct

Name: pyrE [H]

Synonym: Igni_0495

Alternate gene names: 156937289

Gene position: 442365-442931 (Clockwise)

Preceding gene: 156937287

Following gene: 156937290

Centisome position: 34.09

GC content: 57.85

Gene sequence:

>567_bases
ATGGCCGTGCTGAACTTCGGAAGGTGCTACATAAAGTCCTACCTGGCGAGGAGGATTCAAGAGTGCGGGCTGGTAAAGAG
GGGCACCTTCATACTCTCCTCCGGGAAGCGAAGCGACGTTTACGTAGACATGAGGAAGCTTTGGAGCTTTCCCAAGCTCG
CTAGGTTAGCAGCACTGCTCATGTCCGCCCTAATAAGGTCTAAGGTGGAGTATGCGACGATAGTCGGAGTGGCCACCGGC
GGGATACCCCTCGCGGCCCACTTGTCCGTAACCTTGGGTTGGCCCATGGGCTACGCGAGGCCGAAGAGGAAGGAACACGG
TCAAATGAGGCTGGTAGAGGGCGTAGAGCCCGGCCAGAAGGTAGTTGTAGTGGACGACGTGACCACCACCGGCGGCAGCT
TGGCGAAGACCATAGACGCCTTGAGGGACTTCGGGGCAGAGCCTGTCTTGGCGGCCGCCTTGTTGGACAGAGAGGAGGGC
AGCGTAGAGGAGATAGAAAAGAGGGGCGTGGAGTTCATCAGGTTGTTGACTCTTAACCAAGTGTTGAGGGGGTTGAGGTG
CGAGTGA

Upstream 100 bases:

>100_bases
GAATTATATGGAGGCGTTCACTATAGCGTCCTTCGCGGAGCTGCCGTGGACCGGCTCCACGCTCGACGCTGAGGCCTTGC
TGGAAGCGGCCCAAGACCCG

Downstream 100 bases:

>100_bases
TCTGCCCTAAGAAGATGCTCAACGGCCCTTGCGGAGGCGTGAGGGGAGAGCTCTGCGAGGTCGACGACTTCAAGTGCCCC
TTCGTCGAGGCGTTTAGGAA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MAVLNFGRCYIKSYLARRIQECGLVKRGTFILSSGKRSDVYVDMRKLWSFPKLARLAALLMSALIRSKVEYATIVGVATG
GIPLAAHLSVTLGWPMGYARPKRKEHGQMRLVEGVEPGQKVVVVDDVTTTGGSLAKTIDALRDFGAEPVLAAALLDREEG
SVEEIEKRGVEFIRLLTLNQVLRGLRCE

Sequences:

>Translated_188_residues
MAVLNFGRCYIKSYLARRIQECGLVKRGTFILSSGKRSDVYVDMRKLWSFPKLARLAALLMSALIRSKVEYATIVGVATG
GIPLAAHLSVTLGWPMGYARPKRKEHGQMRLVEGVEPGQKVVVVDDVTTTGGSLAKTIDALRDFGAEPVLAAALLDREEG
SVEEIEKRGVEFIRLLTLNQVLRGLRCE
>Mature_187_residues
AVLNFGRCYIKSYLARRIQECGLVKRGTFILSSGKRSDVYVDMRKLWSFPKLARLAALLMSALIRSKVEYATIVGVATGG
IPLAAHLSVTLGWPMGYARPKRKEHGQMRLVEGVEPGQKVVVVDDVTTTGGSLAKTIDALRDFGAEPVLAAALLDREEGS
VEEIEKRGVEFIRLLTLNQVLRGLRCE

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=159, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=5e-21,
Organism=Escherichia coli, GI1790073, Length=192, Percent_Identity=27.0833333333333, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17508631, Length=167, Percent_Identity=34.7305389221557, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17554964, Length=181, Percent_Identity=30.3867403314917, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323927, Length=195, Percent_Identity=27.6923076923077, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323530, Length=160, Percent_Identity=28.125, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17933654, Length=174, Percent_Identity=33.9080459770115, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 20689; Mature: 20557

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLNFGRCYIKSYLARRIQECGLVKRGTFILSSGKRSDVYVDMRKLWSFPKLARLAALL
CCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEHHHHHCCHHHHHHHHHH
MSALIRSKVEYATIVGVATGGIPLAAHLSVTLGWPMGYARPKRKEHGQMRLVEGVEPGQK
HHHHHHHHHHHHHEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCE
VVVVDDVTTTGGSLAKTIDALRDFGAEPVLAAALLDREEGSVEEIEKRGVEFIRLLTLNQ
EEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VLRGLRCE
HHHHCCCC
>Mature Secondary Structure 
AVLNFGRCYIKSYLARRIQECGLVKRGTFILSSGKRSDVYVDMRKLWSFPKLARLAALL
CEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEHHHHHCCHHHHHHHHHH
MSALIRSKVEYATIVGVATGGIPLAAHLSVTLGWPMGYARPKRKEHGQMRLVEGVEPGQK
HHHHHHHHHHHHHEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCE
VVVVDDVTTTGGSLAKTIDALRDFGAEPVLAAALLDREEGSVEEIEKRGVEFIRLLTLNQ
EEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VLRGLRCE
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA