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The map label for this gene is purP

Identifier: 156937236

GI number: 156937236

Start: 392769

End: 393812

Strand: Direct

Name: purP

Synonym: Igni_0442

Alternate gene names: 156937236

Gene position: 392769-393812 (Clockwise)

Preceding gene: 156937233

Following gene: 156937237

Centisome position: 30.27

GC content: 57.38

Gene sequence:

>1044_bases
TTGAGAGTAACCGATGTCTTGAAGGAATACGACCTATCTAAGCTCACCGTAGCCACCGTCGCAAGCCACTCGGCCTTGCA
GATAGTCCACGGCGCCAAGAAGGAGGGCTTGAGGACGCTTTTGATAGTTAAGAAGGATAGGTACGAGTTCTACTCCAGCT
TCTCACACTTGGTTGATTCCTTCATAATAGTTGAGAGCTGGAAGGAGGTGTTGAACGAGGACGTGGTAAACGAGTTGTTG
GCGTCCAACGCAGTGTTGGTGCCCCACGGGAGCTTCGTGGAGTACGTCGGGGCCCAAGGCCTCTTGGACTACCCGGTGCC
GATCTTCGGCTCGAGGAAAATACTCCTCTGGGAGTCGGACCAGAAGAAGAAGATGGAGCTGTTGAGGAGGGCCGGGATAA
AGGTGCCGAGGGAGTACCGAAGCCCCGACGAGGTCGACGGGCTGGTCATAGTCAAGCTCGGAGGGGCCAAGGGAGGCAAG
GGCTACTTCTTGGCCAAGACGCCGGAGGAAGTTAGGAGGGGCTTGGAGGCCTTGGGCAACCCCAGCAACTACATAATTCA
AGAGTACGTGATAGGGGTGCCGGCTTACTACCACTTCTTCTACTCCCCCATTTATAACAGGCTGGAGATAACCGGGATGG
ACATAAGGTACGAGAGCAACGTCGACGGGCTGCGCAGGCTCCCGCCGGAGCTGGCCTCCGGCATAAGGCCGTCCTTCACC
GTAGTGGGCAACATACCCGTGGTTCTGAGGGAGAGCTTGCTAATTGACGTGTACAAGTACGGGAAGAGTTTCGTGAGGAC
GACCGAGGAGCTCTTGGGGGAGGAGCTGGTGGGCCCCTTCTGCCTCGAATCGATAATAACTGAGGAGGGCGAGGTGGTGG
TGTTCGAGTTCTCCGGGAGGATAGTGGCCGGCACGAACTTGTACGTGAACGGCAGCCCCTACTCCTACTTGTACTGGGAC
GAGCCCATGAGCGCCGGGAGGAGGGTGGCGCGGGAGATAAGGAAGGCGAGGGACTCCGGGAGGCTGGAGGAAGTGTTGAG
ATGA

Upstream 100 bases:

>100_bases
CTGTATGGCGCCTTCCAACTCATCGATGGTGAGAGCCAACTCGCTCAGCCGAGCGCTTCTAAAGCGGGGCGGTTTATCAC
TTAAAACAGAGTGAGACGCT

Downstream 100 bases:

>100_bases
GGTGTTTTCGGGGTGCCCGAGGGGCTCCGATGAGGAAGCGGTTACCCCACCGAGGCCTTTTGGGGGAGGCCCGGGGCTCG
GGGGTAGGAAGGGAGCCTTG

Product: 5-formaminoimidazole-4-carboxamide-1-(beta)-D- ribofuranosyl 5'-monophosphate synthetase

Products: NA

Alternate protein names: 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MRVTDVLKEYDLSKLTVATVASHSALQIVHGAKKEGLRTLLIVKKDRYEFYSSFSHLVDSFIIVESWKEVLNEDVVNELL
ASNAVLVPHGSFVEYVGAQGLLDYPVPIFGSRKILLWESDQKKKMELLRRAGIKVPREYRSPDEVDGLVIVKLGGAKGGK
GYFLAKTPEEVRRGLEALGNPSNYIIQEYVIGVPAYYHFFYSPIYNRLEITGMDIRYESNVDGLRRLPPELASGIRPSFT
VVGNIPVVLRESLLIDVYKYGKSFVRTTEELLGEELVGPFCLESIITEEGEVVVFEFSGRIVAGTNLYVNGSPYSYLYWD
EPMSAGRRVAREIRKARDSGRLEEVLR

Sequences:

>Translated_347_residues
MRVTDVLKEYDLSKLTVATVASHSALQIVHGAKKEGLRTLLIVKKDRYEFYSSFSHLVDSFIIVESWKEVLNEDVVNELL
ASNAVLVPHGSFVEYVGAQGLLDYPVPIFGSRKILLWESDQKKKMELLRRAGIKVPREYRSPDEVDGLVIVKLGGAKGGK
GYFLAKTPEEVRRGLEALGNPSNYIIQEYVIGVPAYYHFFYSPIYNRLEITGMDIRYESNVDGLRRLPPELASGIRPSFT
VVGNIPVVLRESLLIDVYKYGKSFVRTTEELLGEELVGPFCLESIITEEGEVVVFEFSGRIVAGTNLYVNGSPYSYLYWD
EPMSAGRRVAREIRKARDSGRLEEVLR
>Mature_347_residues
MRVTDVLKEYDLSKLTVATVASHSALQIVHGAKKEGLRTLLIVKKDRYEFYSSFSHLVDSFIIVESWKEVLNEDVVNELL
ASNAVLVPHGSFVEYVGAQGLLDYPVPIFGSRKILLWESDQKKKMELLRRAGIKVPREYRSPDEVDGLVIVKLGGAKGGK
GYFLAKTPEEVRRGLEALGNPSNYIIQEYVIGVPAYYHFFYSPIYNRLEITGMDIRYESNVDGLRRLPPELASGIRPSFT
VVGNIPVVLRESLLIDVYKYGKSFVRTTEELLGEELVGPFCLESIITEEGEVVVFEFSGRIVAGTNLYVNGSPYSYLYWD
EPMSAGRRVAREIRKARDSGRLEEVLR

Specific function: Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'- monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1- beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates

COG id: COG1759

COG function: function code R; ATP-utilizing enzymes of ATP-grasp superfamily (probably carboligases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURP_IGNH4 (A8A9M3)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435032.1
- ProteinModelPortal:   A8A9M3
- SMR:   A8A9M3
- STRING:   A8A9M3
- GeneID:   5561817
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0442
- eggNOG:   arNOG04185
- HOGENOM:   HBG392202
- OMA:   VFEISAR
- ProtClustDB:   PRK13278
- BioCyc:   IHOS453591:IGNI_0442-MONOMER
- HAMAP:   MF_01163
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR009720
- InterPro:   IPR010672
- InterPro:   IPR013817
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF06849 DUF1246; PF06973 DUF1297

EC number: NA

Molecular weight: Translated: 39170; Mature: 39170

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 23-23 BINDING 91-91 BINDING 218-218 BINDING 244-244

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTDVLKEYDLSKLTVATVASHSALQIVHGAKKEGLRTLLIVKKDRYEFYSSFSHLVDS
CCHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCHHCCCEEEEEEECHHHHHHHHHHHHHHH
FIIVESWKEVLNEDVVNELLASNAVLVPHGSFVEYVGAQGLLDYPVPIFGSRKILLWESD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECC
QKKKMELLRRAGIKVPREYRSPDEVDGLVIVKLGGAKGGKGYFLAKTPEEVRRGLEALGN
CHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHCC
PSNYIIQEYVIGVPAYYHFFYSPIYNRLEITGMDIRYESNVDGLRRLPPELASGIRPSFT
CHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHCCCHHHHCCCCCCEE
VVGNIPVVLRESLLIDVYKYGKSFVRTTEELLGEELVGPFCLESIITEEGEVVVFEFSGR
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCE
IVAGTNLYVNGSPYSYLYWDEPMSAGRRVAREIRKARDSGRLEEVLR
EEECCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHC
>Mature Secondary Structure
MRVTDVLKEYDLSKLTVATVASHSALQIVHGAKKEGLRTLLIVKKDRYEFYSSFSHLVDS
CCHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCHHCCCEEEEEEECHHHHHHHHHHHHHHH
FIIVESWKEVLNEDVVNELLASNAVLVPHGSFVEYVGAQGLLDYPVPIFGSRKILLWESD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECC
QKKKMELLRRAGIKVPREYRSPDEVDGLVIVKLGGAKGGKGYFLAKTPEEVRRGLEALGN
CHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHCC
PSNYIIQEYVIGVPAYYHFFYSPIYNRLEITGMDIRYESNVDGLRRLPPELASGIRPSFT
CHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHCCCHHHHCCCCCCEE
VVGNIPVVLRESLLIDVYKYGKSFVRTTEELLGEELVGPFCLESIITEEGEVVVFEFSGR
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCE
IVAGTNLYVNGSPYSYLYWDEPMSAGRRVAREIRKARDSGRLEEVLR
EEECCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA