Click here to switch to the map view.

The map label for this gene is leuA2 [H]

Identifier: 156937056

GI number: 156937056

Start: 225241

End: 226707

Strand: Direct

Name: leuA2 [H]

Synonym: Igni_0261

Alternate gene names: 156937056

Gene position: 225241-226707 (Clockwise)

Preceding gene: 156937052

Following gene: 156937057

Centisome position: 17.36

GC content: 57.06

Gene sequence:

>1467_bases
ATGTACTACCGTACACGAGAGGCTAGGTCCGAGTTCCTGAGGAAGGTTAGGGAGGAGGCAATAGACTTCCCCGACTACGA
GGTAATAGACTCGCCGTCGCCCGAGCTCTACAAGTTCATTTTTCCCTTCAAGGAAGTGCCGCGCGTGTTCTTCGACGGCA
TAATACTCCCTACGGAGCTGCCCTCAGAGATATGGATAACGGACACCACCTTCAGAGACGGCCAGCAAGCCCGGGAGCCC
TACACGGTCGAGCAGATAGTGCGCTTGTACAAGTACTTACACGAGCTCGGAGGCCCCTTAGGGAAGATTAAGACCACAGA
GTTCTTCCTGTATACAGAGAAGGACAAGGAGGCAGTGAGGAGGGTAAAGGAGCTGGGCTACAAATATCCGATCCCCACCG
GGTGGATAAGGGCGAAGAAGGAAGAGCTCAGCTTGGTTAAGGAGGCCGGCTTGGAGGAGACCGGAATACTCGCCTCCATA
AGCGACTACCACATATACTTCAAGTTTAAGGCTTCCAGGGGCAAAGTCGTAGAGAAGTACTTGAGCGTCGTGGAGGAGGC
CCTTAAGATGGACATAATACCCAGGGTGCACTTGGAGGACATAACTAGGGCAAACATATTCGAAGTGGTAGTCCCGTTCG
TCCGAAAGTTAATGAAGTTGAGCGAGAGGTACGGGCTCCCGGTGAAGGTTAGGTACCCCGACACGCTGGGCGTCGGCGTG
CCCATAGTGGAGGCGATGTTGCCTAGGAGCATCCCCAAGCTAACTTGGGTCCTCCGCCACGTCTGCGGCGTGCCGAGCGA
GTGGCTGGAGTTCCACGGCCACAACGACTTCCACTTAGGAGTGGCTAACGCGGTGAGCGCTTGGCTCTACGGCGCCGGGC
TGAACAACGGCACGCTGTTAGGGATAGGCGAGAGGGCGGGCAACGTGCCGATAGAGGCCTTAGTCTTCGCCTACGCCCAG
CTGAAGCACGGGTTTGACGGGATGAACACTAAGGTGATAACCGAGATAGCCAAGTACTACCGCGACGAGCTGGGTTACGA
GGTACCGCCTTACTACCCCATAGTAGGGAGGAACTTCGCCGTGACGAGGGCCGGAATCCACGCGGACGGGCTCTTGAAGA
ACCCGGAGACCTACTTGCCCTTCGACACGGAAGGGATCTTGGGCGTGAAGCCCGGAGTCGCCATAACTCCCTACGCGGGC
TTGGCCGGCATAGTCTACTGGATAAACAAGACTTTCGACCTAAAGGGGGAGGAACAAGTGAGCAAGAAGGACCCGAAGGT
GCAAGCGATATACAAGGACGTCATGAAGCAGTTCGAGCACGGCCGCTTGACTGCCTTGAGCGACGCCGAGATGCTGGCGC
TGGTCGCCAAGCACGCGCCGGAGCTCATCAAGAAGTACTGGGACAGGCTCCCGGAGGACTTGAAGGCCAAAGCGGTGGCG
CTGCTGGACCTCGCGGAGAGGAGATAA

Upstream 100 bases:

>100_bases
CGCCTCCGCCTTCGTTACGCCCCCGCGGGATTTGAGGGGAGCGGTCGTCCGCGCCCAAGCTTATATAAATGGAACGGGTA
ACTGGTAGGGGCTCCGCAAC

Downstream 100 bases:

>100_bases
GAGCTTTTCCTCTCCCAGTAAGCCGGGACCCCCAGTGAGCTTGTTGGAGAAGGTCGAGTTAGCCCTTTCTATAACCTTCA
TAATATTGTTAATAGCCTTG

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MYYRTREARSEFLRKVREEAIDFPDYEVIDSPSPELYKFIFPFKEVPRVFFDGIILPTELPSEIWITDTTFRDGQQAREP
YTVEQIVRLYKYLHELGGPLGKIKTTEFFLYTEKDKEAVRRVKELGYKYPIPTGWIRAKKEELSLVKEAGLEETGILASI
SDYHIYFKFKASRGKVVEKYLSVVEEALKMDIIPRVHLEDITRANIFEVVVPFVRKLMKLSERYGLPVKVRYPDTLGVGV
PIVEAMLPRSIPKLTWVLRHVCGVPSEWLEFHGHNDFHLGVANAVSAWLYGAGLNNGTLLGIGERAGNVPIEALVFAYAQ
LKHGFDGMNTKVITEIAKYYRDELGYEVPPYYPIVGRNFAVTRAGIHADGLLKNPETYLPFDTEGILGVKPGVAITPYAG
LAGIVYWINKTFDLKGEEQVSKKDPKVQAIYKDVMKQFEHGRLTALSDAEMLALVAKHAPELIKKYWDRLPEDLKAKAVA
LLDLAERR

Sequences:

>Translated_488_residues
MYYRTREARSEFLRKVREEAIDFPDYEVIDSPSPELYKFIFPFKEVPRVFFDGIILPTELPSEIWITDTTFRDGQQAREP
YTVEQIVRLYKYLHELGGPLGKIKTTEFFLYTEKDKEAVRRVKELGYKYPIPTGWIRAKKEELSLVKEAGLEETGILASI
SDYHIYFKFKASRGKVVEKYLSVVEEALKMDIIPRVHLEDITRANIFEVVVPFVRKLMKLSERYGLPVKVRYPDTLGVGV
PIVEAMLPRSIPKLTWVLRHVCGVPSEWLEFHGHNDFHLGVANAVSAWLYGAGLNNGTLLGIGERAGNVPIEALVFAYAQ
LKHGFDGMNTKVITEIAKYYRDELGYEVPPYYPIVGRNFAVTRAGIHADGLLKNPETYLPFDTEGILGVKPGVAITPYAG
LAGIVYWINKTFDLKGEEQVSKKDPKVQAIYKDVMKQFEHGRLTALSDAEMLALVAKHAPELIKKYWDRLPEDLKAKAVA
LLDLAERR
>Mature_488_residues
MYYRTREARSEFLRKVREEAIDFPDYEVIDSPSPELYKFIFPFKEVPRVFFDGIILPTELPSEIWITDTTFRDGQQAREP
YTVEQIVRLYKYLHELGGPLGKIKTTEFFLYTEKDKEAVRRVKELGYKYPIPTGWIRAKKEELSLVKEAGLEETGILASI
SDYHIYFKFKASRGKVVEKYLSVVEEALKMDIIPRVHLEDITRANIFEVVVPFVRKLMKLSERYGLPVKVRYPDTLGVGV
PIVEAMLPRSIPKLTWVLRHVCGVPSEWLEFHGHNDFHLGVANAVSAWLYGAGLNNGTLLGIGERAGNVPIEALVFAYAQ
LKHGFDGMNTKVITEIAKYYRDELGYEVPPYYPIVGRNFAVTRAGIHADGLLKNPETYLPFDTEGILGVKPGVAITPYAG
LAGIVYWINKTFDLKGEEQVSKKDPKVQAIYKDVMKQFEHGRLTALSDAEMLALVAKHAPELIKKYWDRLPEDLKAKAVA
LLDLAERR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=359, Percent_Identity=22.8412256267409, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6320019, Length=324, Percent_Identity=25, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320071, Length=326, Percent_Identity=25.1533742331288, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55664; Mature: 55664

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYRTREARSEFLRKVREEAIDFPDYEVIDSPSPELYKFIFPFKEVPRVFFDGIILPTEL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCEEECCCC
PSEIWITDTTFRDGQQAREPYTVEQIVRLYKYLHELGGPLGKIKTTEFFLYTEKDKEAVR
CCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHHH
RVKELGYKYPIPTGWIRAKKEELSLVKEAGLEETGILASISDYHIYFKFKASRGKVVEKY
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEEEEEECCCCHHHHHH
LSVVEEALKMDIIPRVHLEDITRANIFEVVVPFVRKLMKLSERYGLPVKVRYPDTLGVGV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCH
PIVEAMLPRSIPKLTWVLRHVCGVPSEWLEFHGHNDFHLGVANAVSAWLYGAGLNNGTLL
HHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCCEEE
GIGERAGNVPIEALVFAYAQLKHGFDGMNTKVITEIAKYYRDELGYEVPPYYPIVGRNFA
EECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
VTRAGIHADGLLKNPETYLPFDTEGILGVKPGVAITPYAGLAGIVYWINKTFDLKGEEQV
EEECCCCCCCCCCCCCCCCCCCCCCCEECCCCCEECCCHHHHHHHHHHCCEECCCCCHHH
SKKDPKVQAIYKDVMKQFEHGRLTALSDAEMLALVAKHAPELIKKYWDRLPEDLKAKAVA
HHCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LLDLAERR
HHHHHHCC
>Mature Secondary Structure
MYYRTREARSEFLRKVREEAIDFPDYEVIDSPSPELYKFIFPFKEVPRVFFDGIILPTEL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCEEECCCC
PSEIWITDTTFRDGQQAREPYTVEQIVRLYKYLHELGGPLGKIKTTEFFLYTEKDKEAVR
CCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHHH
RVKELGYKYPIPTGWIRAKKEELSLVKEAGLEETGILASISDYHIYFKFKASRGKVVEKY
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEEEEEECCCCHHHHHH
LSVVEEALKMDIIPRVHLEDITRANIFEVVVPFVRKLMKLSERYGLPVKVRYPDTLGVGV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCH
PIVEAMLPRSIPKLTWVLRHVCGVPSEWLEFHGHNDFHLGVANAVSAWLYGAGLNNGTLL
HHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCCEEE
GIGERAGNVPIEALVFAYAQLKHGFDGMNTKVITEIAKYYRDELGYEVPPYYPIVGRNFA
EECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
VTRAGIHADGLLKNPETYLPFDTEGILGVKPGVAITPYAGLAGIVYWINKTFDLKGEEQV
EEECCCCCCCCCCCCCCCCCCCCCCCEECCCCCEECCCHHHHHHHHHHCCEECCCCCHHH
SKKDPKVQAIYKDVMKQFEHGRLTALSDAEMLALVAKHAPELIKKYWDRLPEDLKAKAVA
HHCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LLDLAERR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]