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The map label for this gene is acsA [H]

Identifier: 156937051

GI number: 156937051

Start: 221174

End: 222412

Strand: Direct

Name: acsA [H]

Synonym: Igni_0256

Alternate gene names: 156937051

Gene position: 221174-222412 (Clockwise)

Preceding gene: 156937050

Following gene: 156937052

Centisome position: 17.05

GC content: 58.43

Gene sequence:

>1239_bases
TTGACCGAAGAACAGAAGACTGTGTTACCGATCAGTAAGGGCGTGGTCCCTACTGGAAAGCTCCACGAGATGAGGAAGAG
GGCCCTCGAGGACCCGGAGGGCTTCTGGTCGGAGATCGCCTCGGAGCTCTATTGGAAGAAGGGTTGGGATAAGGTAGTCG
AGGGCGAGGCCCCCTACTTCAAGTGGTTCGTGGGCGGGAAGACCAACATATCCTACAACGCCTTAGACAGGCACGTTAAG
ACTTGGAGGAGGAACAAGGCCGCCATAATCTGGGAGGGCGAGCCCGGCGAGACCAGGGTCATGACTTATTACCAGCTCTG
GAAGGAAGTAAACCGCTTCGCCAACGTGCTGAAGGATTTGGGCGTTAAGAAGGGAGACAGAGTGACCATTTACATGCCCA
TGGTTCCCGAGGCAGTCGTCGCTATGCTGGCGACCGTGAGGGTCGGCGCGATCCACAGCGTGGTCTTCTCCGGGTTCTCT
GCGAAGGGGTTGGCGGAGAGGATAAACGACGCTCAAGCTAAGGTCGTGATAACGGCCGACGGCATGTGGAGGAGGGGGAA
GAAGCTCATACTCAAGGAAATAGTGGACAAGGCTTTGGAGGAGACGCCGTCCGTGGAGAGCGTGGTGGTTTACAAGAGGC
TGGGCGAGGAGGTCAACATGGTCGAGGGCAGGGACTACTGGTGGGACGACCTCATGGAGGGCGCGGAGGACTACGCGGAG
CCCGAGTGGGTGGAGAGCGAGCACCCCTCCTTCATCCTCTACACTTCCGGCACCACCGGCAAGCCCAAGGGGATAATTCA
CGACACCGGGGGCTACATGGTAGGAGTCTACGCTACGATGAAGTGGGTATTCGACATAAGGGACGACGACTTGCACTGGT
GTACTGCAGACATAGGCTGGATCACCGGCCACTCCTACGTGGTCTACGGCCCCTTGCTCCACGGGGCCACTACCCTCATG
TACGAGGGCGCCCCCGACTACCCCAAGCCCGACCGCTGGTGGAGCATAATTGAGAAGTACGGTGTAACTACCTTCTACAC
GGCTCCCACGGCTATAAGGATGTTCATGAGGTATGGCGAGAAGTGGGTGGAGAAGCACGACCTCAGCACCTTGAGGATCT
TGGGCAGCGTCGGCGAGCCCATAAACCCGGAGGCGTGGTGGTGGTACTTCAAGCACGTCGGCAAGGGCAAGTGTCCCATA
GTAGACACTTGGTGGCAGACGGAGACCGGGCACACATAG

Upstream 100 bases:

>100_bases
TTCAGATAAGGTGCTTAGGATAAGCAACGTACCGGTGCTCATCGTAAAGGGCTAAAGGTAGATTAGGCCTCTGAGCGGTA
GAAAGTCATGAGGTGTGTCC

Downstream 100 bases:

>100_bases
TAGACACTTGGTGGCAGACGGAGACCGGGCACATAATGATAAGCCCCGCCCCCGGGATAGAGCTGGTCCCCCTGAAGCCG
GGGAGCGCCACCTTCCCGTT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MTEEQKTVLPISKGVVPTGKLHEMRKRALEDPEGFWSEIASELYWKKGWDKVVEGEAPYFKWFVGGKTNISYNALDRHVK
TWRRNKAAIIWEGEPGETRVMTYYQLWKEVNRFANVLKDLGVKKGDRVTIYMPMVPEAVVAMLATVRVGAIHSVVFSGFS
AKGLAERINDAQAKVVITADGMWRRGKKLILKEIVDKALEETPSVESVVVYKRLGEEVNMVEGRDYWWDDLMEGAEDYAE
PEWVESEHPSFILYTSGTTGKPKGIIHDTGGYMVGVYATMKWVFDIRDDDLHWCTADIGWITGHSYVVYGPLLHGATTLM
YEGAPDYPKPDRWWSIIEKYGVTTFYTAPTAIRMFMRYGEKWVEKHDLSTLRILGSVGEPINPEAWWWYFKHVGKGKCPI
VDTWWQTETGHT

Sequences:

>Translated_412_residues
MTEEQKTVLPISKGVVPTGKLHEMRKRALEDPEGFWSEIASELYWKKGWDKVVEGEAPYFKWFVGGKTNISYNALDRHVK
TWRRNKAAIIWEGEPGETRVMTYYQLWKEVNRFANVLKDLGVKKGDRVTIYMPMVPEAVVAMLATVRVGAIHSVVFSGFS
AKGLAERINDAQAKVVITADGMWRRGKKLILKEIVDKALEETPSVESVVVYKRLGEEVNMVEGRDYWWDDLMEGAEDYAE
PEWVESEHPSFILYTSGTTGKPKGIIHDTGGYMVGVYATMKWVFDIRDDDLHWCTADIGWITGHSYVVYGPLLHGATTLM
YEGAPDYPKPDRWWSIIEKYGVTTFYTAPTAIRMFMRYGEKWVEKHDLSTLRILGSVGEPINPEAWWWYFKHVGKGKCPI
VDTWWQTETGHT
>Mature_411_residues
TEEQKTVLPISKGVVPTGKLHEMRKRALEDPEGFWSEIASELYWKKGWDKVVEGEAPYFKWFVGGKTNISYNALDRHVKT
WRRNKAAIIWEGEPGETRVMTYYQLWKEVNRFANVLKDLGVKKGDRVTIYMPMVPEAVVAMLATVRVGAIHSVVFSGFSA
KGLAERINDAQAKVVITADGMWRRGKKLILKEIVDKALEETPSVESVVVYKRLGEEVNMVEGRDYWWDDLMEGAEDYAEP
EWVESEHPSFILYTSGTTGKPKGIIHDTGGYMVGVYATMKWVFDIRDDDLHWCTADIGWITGHSYVVYGPLLHGATTLMY
EGAPDYPKPDRWWSIIEKYGVTTFYTAPTAIRMFMRYGEKWVEKHDLSTLRILGSVGEPINPEAWWWYFKHVGKGKCPIV
DTWWQTETGHT

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI8923896, Length=427, Percent_Identity=49.4145199063232, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI253970489, Length=440, Percent_Identity=47.9545454545455, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI28416953, Length=396, Percent_Identity=46.969696969697, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI13375727, Length=392, Percent_Identity=40.3061224489796, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI31982927, Length=378, Percent_Identity=28.8359788359788, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI42544134, Length=299, Percent_Identity=25.752508361204, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI42544132, Length=299, Percent_Identity=25.752508361204, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI38505220, Length=288, Percent_Identity=24.6527777777778, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI122937307, Length=308, Percent_Identity=24.3506493506494, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI58082049, Length=356, Percent_Identity=24.438202247191, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI115511026, Length=290, Percent_Identity=23.1034482758621, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI195947378, Length=322, Percent_Identity=22.6708074534161, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI157311624, Length=360, Percent_Identity=24.1666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157311622, Length=360, Percent_Identity=24.1666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790505, Length=420, Percent_Identity=50, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1786529, Length=394, Percent_Identity=39.3401015228426, Blast_Score=309, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI71982997, Length=410, Percent_Identity=46.8292682926829, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71983001, Length=410, Percent_Identity=46.8292682926829, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17569507, Length=379, Percent_Identity=21.6358839050132, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=401, Percent_Identity=48.8778054862843, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323182, Length=394, Percent_Identity=46.4467005076142, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24667955, Length=411, Percent_Identity=50.6082725060827, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI62472339, Length=328, Percent_Identity=56.0975609756098, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24667959, Length=294, Percent_Identity=55.1020408163265, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI21355225, Length=397, Percent_Identity=36.7758186397985, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011904
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 47296; Mature: 47164

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEQKTVLPISKGVVPTGKLHEMRKRALEDPEGFWSEIASELYWKKGWDKVVEGEAPYF
CCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCEE
KWFVGGKTNISYNALDRHVKTWRRNKAAIIWEGEPGETRVMTYYQLWKEVNRFANVLKDL
EEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHC
GVKKGDRVTIYMPMVPEAVVAMLATVRVGAIHSVVFSGFSAKGLAERINDAQAKVVITAD
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEEC
GMWRRGKKLILKEIVDKALEETPSVESVVVYKRLGEEVNMVEGRDYWWDDLMEGAEDYAE
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
PEWVESEHPSFILYTSGTTGKPKGIIHDTGGYMVGVYATMKWVFDIRDDDLHWCTADIGW
CCCCCCCCCCEEEEECCCCCCCCCEEECCCCEEEEHEEEEEEEEEECCCCCEEEEEECCE
ITGHSYVVYGPLLHGATTLMYEGAPDYPKPDRWWSIIEKYGVTTFYTAPTAIRMFMRYGE
EECCCEEEEECHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCEEEEECHHHHHHHHHHHH
KWVEKHDLSTLRILGSVGEPINPEAWWWYFKHVGKGKCPIVDTWWQTETGHT
HHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TEEQKTVLPISKGVVPTGKLHEMRKRALEDPEGFWSEIASELYWKKGWDKVVEGEAPYF
CCCCCEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCEE
KWFVGGKTNISYNALDRHVKTWRRNKAAIIWEGEPGETRVMTYYQLWKEVNRFANVLKDL
EEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHC
GVKKGDRVTIYMPMVPEAVVAMLATVRVGAIHSVVFSGFSAKGLAERINDAQAKVVITAD
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEEC
GMWRRGKKLILKEIVDKALEETPSVESVVVYKRLGEEVNMVEGRDYWWDDLMEGAEDYAE
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
PEWVESEHPSFILYTSGTTGKPKGIIHDTGGYMVGVYATMKWVFDIRDDDLHWCTADIGW
CCCCCCCCCCEEEEECCCCCCCCCEEECCCCEEEEHEEEEEEEEEECCCCCEEEEEECCE
ITGHSYVVYGPLLHGATTLMYEGAPDYPKPDRWWSIIEKYGVTTFYTAPTAIRMFMRYGE
EECCCEEEEECHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCEEEEECHHHHHHHHHHHH
KWVEKHDLSTLRILGSVGEPINPEAWWWYFKHVGKGKCPIVDTWWQTETGHT
HHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA