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The map label for this gene is pyrB

Identifier: 156936839

GI number: 156936839

Start: 41646

End: 42563

Strand: Reverse

Name: pyrB

Synonym: Igni_0044

Alternate gene names: 156936839

Gene position: 42563-41646 (Counterclockwise)

Preceding gene: 156936841

Following gene: 156936838

Centisome position: 3.28

GC content: 49.35

Gene sequence:

>918_bases
TTGCCAGACGTCGTAGACGTCTTGGATTTAGATAAGAAAGACATGATCAAAATATTCGAACTCGCTGAGGACATAGAATA
TAAGAAACTTCATAAGTCGAAGTGGTGTGCCCTTGAAAACAAAACAGTGTCACTCCTTTTCGTCGAACCGTCCACTAGAA
CGAGACTTTCGTTCGAAGAGAGCGCCAAGAGGTTGTGTGCCCACACGCTCACAATAGTCGGCGAGGAAGCCAGTTCGTTC
GTCAAAGGTGAGAGCCTATACGACACTATAAAAGTCTTAGACAAGATTAGCGACATAATAGTGATCAGACACAACTTGGA
CGGCTCTGCGAAGTACGCAAGCGAGGTCGCCGCAAACCCAGTAATTAACGCAGGCGACGGCAAGAACCAACACCCAACGC
AGTCGCTCATAGACCTCTACGTAGTCAGAAAGAGGAAAGGGGGGCTCGAAGACCTTCGTTACGCTGTCGTGGGGGACCTT
CGGTACGCTAGGACGGCGAGGAGCTTCCTGTTGGCGTTGACTAAGTTTAAACCTAGGTCAGTCTACCTAGTAGCCCCCGA
AGTTCTCAAACCCTCAATCAAGTTCTTAGAAGAGTTAAAATCTATGGGTCTCAACGTTTTTGAAGTAGATAGGTTGGAGG
ATGTCATTCCTAACGTCGACGTAATATACGTAACCAGAATACAGAAGGAGAGGTTTCCGGACCCCTCAGAATACGAGAAG
GTTAAAGGTAGCTACAAAATAACCTTAGACCTGATAAAGAGGGGGAAGGGGGACTTGATCGTCTTACACCCGCTCCCCCG
TGTGGACGAGTTGGACGTGAGAATAGACTCGACCCCCCACGCAGCTTACTTCGACCAAGTGGCTGCTTCCGTGCCAGTGA
GAATGGCAACGCTCGCTTGGAGTGCGGGGGTGGTCTGA

Upstream 100 bases:

>100_bases
CACTTCCCTCGGTTCCTTCGTGGAGATCTGAAGGGTCTTTGAAAGCTTGACGAAAAATCGCGTTTTTCTACCTCCCATCC
CAAGGCAGTCCGGGCGTTAG

Downstream 100 bases:

>100_bases
GGTGAAGGAGCTAAAAGTAAGCAAGATCAACAACGGCACCGTAATAGACCACTTGCCTCCCGGAAGGGCACTAAAGGTCT
TGAGGCTCCTCGGCATAGAC

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MPDVVDVLDLDKKDMIKIFELAEDIEYKKLHKSKWCALENKTVSLLFVEPSTRTRLSFEESAKRLCAHTLTIVGEEASSF
VKGESLYDTIKVLDKISDIIVIRHNLDGSAKYASEVAANPVINAGDGKNQHPTQSLIDLYVVRKRKGGLEDLRYAVVGDL
RYARTARSFLLALTKFKPRSVYLVAPEVLKPSIKFLEELKSMGLNVFEVDRLEDVIPNVDVIYVTRIQKERFPDPSEYEK
VKGSYKITLDLIKRGKGDLIVLHPLPRVDELDVRIDSTPHAAYFDQVAASVPVRMATLAWSAGVV

Sequences:

>Translated_305_residues
MPDVVDVLDLDKKDMIKIFELAEDIEYKKLHKSKWCALENKTVSLLFVEPSTRTRLSFEESAKRLCAHTLTIVGEEASSF
VKGESLYDTIKVLDKISDIIVIRHNLDGSAKYASEVAANPVINAGDGKNQHPTQSLIDLYVVRKRKGGLEDLRYAVVGDL
RYARTARSFLLALTKFKPRSVYLVAPEVLKPSIKFLEELKSMGLNVFEVDRLEDVIPNVDVIYVTRIQKERFPDPSEYEK
VKGSYKITLDLIKRGKGDLIVLHPLPRVDELDVRIDSTPHAAYFDQVAASVPVRMATLAWSAGVV
>Mature_304_residues
PDVVDVLDLDKKDMIKIFELAEDIEYKKLHKSKWCALENKTVSLLFVEPSTRTRLSFEESAKRLCAHTLTIVGEEASSFV
KGESLYDTIKVLDKISDIIVIRHNLDGSAKYASEVAANPVINAGDGKNQHPTQSLIDLYVVRKRKGGLEDLRYAVVGDLR
YARTARSFLLALTKFKPRSVYLVAPEVLKPSIKFLEELKSMGLNVFEVDRLEDVIPNVDVIYVTRIQKERFPDPSEYEKV
KGSYKITLDLIKRGKGDLIVLHPLPRVDELDVRIDSTPHAAYFDQVAASVPVRMATLAWSAGVV

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=297, Percent_Identity=35.3535353535354, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI2367364, Length=264, Percent_Identity=43.9393939393939, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1790703, Length=226, Percent_Identity=28.7610619469027, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=315, Percent_Identity=40, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=302, Percent_Identity=33.4437086092715, Blast_Score=180, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24642586, Length=266, Percent_Identity=39.0977443609023, Blast_Score=184, Evalue=6e-47,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_IGNH4 (A8A8H6)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001434635.1
- ProteinModelPortal:   A8A8H6
- STRING:   A8A8H6
- GeneID:   5562632
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0044
- eggNOG:   arNOG04260
- HOGENOM:   HBG579429
- OMA:   LASEFAC
- BioCyc:   IHOS453591:IGNI_0044-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34345; Mature: 34214

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDVVDVLDLDKKDMIKIFELAEDIEYKKLHKSKWCALENKTVSLLFVEPSTRTRLSFEE
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEECCCCCCEECHHH
SAKRLCAHTLTIVGEEASSFVKGESLYDTIKVLDKISDIIVIRHNLDGSAKYASEVAANP
HHHHHHHHHHHEECCHHHHHHCCHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHCCC
VINAGDGKNQHPTQSLIDLYVVRKRKGGLEDLRYAVVGDLRYARTARSFLLALTKFKPRS
CEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYLVAPEVLKPSIKFLEELKSMGLNVFEVDRLEDVIPNVDVIYVTRIQKERFPDPSEYEK
EEEECHHHHCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCEEEEEEEHHHCCCCCHHHHH
VKGSYKITLDLIKRGKGDLIVLHPLPRVDELDVRIDSTPHAAYFDQVAASVPVRMATLAW
HCCCEEEEEEHHHCCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHH
SAGVV
HCCCC
>Mature Secondary Structure 
PDVVDVLDLDKKDMIKIFELAEDIEYKKLHKSKWCALENKTVSLLFVEPSTRTRLSFEE
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEECCCCCCEECHHH
SAKRLCAHTLTIVGEEASSFVKGESLYDTIKVLDKISDIIVIRHNLDGSAKYASEVAANP
HHHHHHHHHHHEECCHHHHHHCCHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHCCC
VINAGDGKNQHPTQSLIDLYVVRKRKGGLEDLRYAVVGDLRYARTARSFLLALTKFKPRS
CEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYLVAPEVLKPSIKFLEELKSMGLNVFEVDRLEDVIPNVDVIYVTRIQKERFPDPSEYEK
EEEECHHHHCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCEEEEEEEHHHCCCCCHHHHH
VKGSYKITLDLIKRGKGDLIVLHPLPRVDELDVRIDSTPHAAYFDQVAASVPVRMATLAW
HCCCEEEEEEHHHCCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHH
SAGVV
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA