Click here to switch to the map view.

The map label for this gene is pyrI

Identifier: 156936838

GI number: 156936838

Start: 41171

End: 41644

Strand: Reverse

Name: pyrI

Synonym: Igni_0043

Alternate gene names: 156936838

Gene position: 41644-41171 (Counterclockwise)

Preceding gene: 156936839

Following gene: 156936837

Centisome position: 3.21

GC content: 47.05

Gene sequence:

>474_bases
GTGAAGGAGCTAAAAGTAAGCAAGATCAACAACGGCACCGTAATAGACCACTTGCCTCCCGGAAGGGCACTAAAGGTCTT
GAGGCTCCTCGGCATAGACGGTAGCGAAGGCTTTATGGTTCTCATAGCTATGAACGTCTACTCAAAGAAGTTGGGCGGGA
GAAAAGACATAGTTAAAATAGAGAACGTTTACCTAAGCGACGAGCAGACCAAGGCGTTGGCCTTAATAGCGCCCACCGCG
ACGATTAACATAATAAGGAACGGAGAAGTAGTGGAGAAAAGAGGCGTCACGCTTCCCGACGTGGTCGAGGGTATCTTAAC
TTGTCCTAACAGTTCGTGTATTTCCCGGTCTAAGAGGGAGCCTATAAAGTCTAAGTTCTTGGTCGTCTCGAAGAGGCCGC
TCAAACTGAAGTGTCACTACTGCGGAGAAATAATAGAAGAGAAAGAGGTCTCTAATTACATAAACTTGAGGTGA

Upstream 100 bases:

>100_bases
AGAATAGACTCGACCCCCCACGCAGCTTACTTCGACCAAGTGGCTGCTTCCGTGCCAGTGAGAATGGCAACGCTCGCTTG
GAGTGCGGGGGTGGTCTGAG

Downstream 100 bases:

>100_bases
CATAGCATGAGCTTCAAGGTAGAGGAGGTCTTGAGGAGTCGTAAGCTCGCGAAGGGAATCTACGAAACGTTCGTGAGCTA
TCATGGAGAAGCTCCCCCTC

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MKELKVSKINNGTVIDHLPPGRALKVLRLLGIDGSEGFMVLIAMNVYSKKLGGRKDIVKIENVYLSDEQTKALALIAPTA
TINIIRNGEVVEKRGVTLPDVVEGILTCPNSSCISRSKREPIKSKFLVVSKRPLKLKCHYCGEIIEEKEVSNYINLR

Sequences:

>Translated_157_residues
MKELKVSKINNGTVIDHLPPGRALKVLRLLGIDGSEGFMVLIAMNVYSKKLGGRKDIVKIENVYLSDEQTKALALIAPTA
TINIIRNGEVVEKRGVTLPDVVEGILTCPNSSCISRSKREPIKSKFLVVSKRPLKLKCHYCGEIIEEKEVSNYINLR
>Mature_157_residues
MKELKVSKINNGTVIDHLPPGRALKVLRLLGIDGSEGFMVLIAMNVYSKKLGGRKDIVKIENVYLSDEQTKALALIAPTA
TINIIRNGEVVEKRGVTLPDVVEGILTCPNSSCISRSKREPIKSKFLVVSKRPLKLKCHYCGEIIEEKEVSNYINLR

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=155, Percent_Identity=42.5806451612903, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_IGNH4 (A8A8H5)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001434634.1
- ProteinModelPortal:   A8A8H5
- SMR:   A8A8H5
- STRING:   A8A8H5
- GeneID:   5562638
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0043
- eggNOG:   arNOG10217
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- BioCyc:   IHOS453591:IGNI_0043-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17430; Mature: 17430

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELKVSKINNGTVIDHLPPGRALKVLRLLGIDGSEGFMVLIAMNVYSKKLGGRKDIVKI
CCCCEEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECHHHHHCCCCCCEEEE
ENVYLSDEQTKALALIAPTATINIIRNGEVVEKRGVTLPDVVEGILTCPNSSCISRSKRE
EEEEECCCCCCEEEEEECCEEEEEEECCCEEECCCCCCHHHHHHHHCCCCCHHHHCCCCC
PIKSKFLVVSKRPLKLKCHYCGEIIEEKEVSNYINLR
CCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKELKVSKINNGTVIDHLPPGRALKVLRLLGIDGSEGFMVLIAMNVYSKKLGGRKDIVKI
CCCCEEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECHHHHHCCCCCCEEEE
ENVYLSDEQTKALALIAPTATINIIRNGEVVEKRGVTLPDVVEGILTCPNSSCISRSKRE
EEEEECCCCCCEEEEEECCEEEEEEECCCEEECCCCCCHHHHHHHHCCCCCHHHHCCCCC
PIKSKFLVVSKRPLKLKCHYCGEIIEEKEVSNYINLR
CCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA