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The map label for this gene is infB

Identifier: 156936796

GI number: 156936796

Start: 143

End: 1972

Strand: Reverse

Name: infB

Synonym: Igni_0001

Alternate gene names: 156936796

Gene position: 1972-143 (Counterclockwise)

Preceding gene: 156936797

Following gene: NA

Centisome position: 0.15

GC content: 53.39

Gene sequence:

>1830_bases
GTGTTGAACTTGAGCGCGGAGCAGAGGCCTAGGCTGAGGCAACCTATCGTAGCCGTACTGGGTCACGTGGACCACGGAAA
GACTACGCTGTTGGACAAGATAAGGGGTACCGTAGTAGCCTTGAAGGAGCCCGGCCAGATAACCCAACACATAGGCGCTA
GCCTAGTTCCCACAGACGTCATAGAGAAAGTTACGGAACCGCTAAAGAAGATAATCCCTACGGTAAAGCTGGAACTCCCC
GGGCTGCTGTTCATTGACACCCCAGGCCACGAGATATTCTCGAACTTGAGGAGAAGGGGCGGCGCGGTCGCGGACTTGGC
GATACTCGTCGTTGACTTAAACGAGGGCTTTCAGCCGCAGACGTACGAAGCCGTGGAGATCCTCAAGCAGAGGCGAGTTC
CGTTCGTGGTAGCGGCGAACAAAATAGACCGGATACCCGGCTGGAGGTCCTACCCCGACCAACCATTCTTGATTAGCTAT
AGAAAGCAGAGCGAGGAGGTGAGGGAGAGACTAGACAACAAGATATACGAAATTATGGGGGAGCTGGCAAAGCTCGGCTT
TGACAGTGAACGATTCGATAGGATAACTAACTTCACTAGACAGATAGCGATAGTCCCGATATCCGCTAAGACCGGTGAGG
GGATACCGGAGCTGTTAGCGGTGCTCGCTGGCTTGGCCCAGAGGTACATGAAGGGGAGGCTTAAGTACGTAGAGGGCCCG
GCCAAGGGAGTTATTATGGAAGTTAAGGAGGAGCCGGGTTACGGCTCTACCATAGACACGATAATTTATGATGGCATAAT
AAGGCAAGGAGATACTATAGTCGTCGGCGGAATAGAAGGACCCATCGTGACTAAGGTGAGGGCCCTACTCGTCCCTGCCC
CCTTGACCGAGATGAGGGCCACTAAGAGGTTCGAACCCGTGGAGGAGGTGAGCGCCGCAGCGGGCGTGAAGATAGTGGCG
CCCGGCTTGGAGAAGGCGGTGGCCGGCGCACCGGTCTACGTCGTGGACAGCCCGGAGAAGCTTGAAGAGCTGAAAGAACA
AGTAAAGAGAGAAGTAGAAGAGGTAATGATAGAGACGGATAAGGAAGGCGTGATAGTTAAGGCAGACACCCTCGGAACTC
TCGAGGCGTTGGTACAGTTCTTGAAGAACAGGGGCATACCGGTTAGAATGGCCAGGGTTGGGCCTGTCACTAAGAGAGAC
ATAGTAGAGGCTATGACTGTCAAGAGTAAGAACAAAGAATATGGAGTGATACTAGCGTTCAACGTTAAAGTTTTGCCAGA
AGCAAAGGAACTGGCGGAGAAGGAGGGCATAAAGATATTCCAGCACAACGTGATATACAGACTAATAGAGGAATTCGAGG
AGTGGTTGAAGGCCCTCCGCGAGGAGGAGAAGAGAAAGGTCTTGGAAACCCTCGTGAGACCCGGGAAGATAAGGATACTC
CCCGGCTTCGTGTTTAGAAGGAGTGACCCGGCGATAGTAGGCGTCGAGGTTCTGGGAGGCGTGATAAAACCGAAGTACCC
GCTGATGAAGGAGGACGGCTCTAGGGTTGGCAGCATACTCCAGATCCAAGATAAGGGCCAGAGCGTCCCCGAGGCGAGGG
CTGGTCAACAAGTAGCTATTTCAATAAAGGGAAGGGTCATGGTCGGGCGACACATACACGAGGGCGACGTGTTGTACACG
GACGTCCCGGCGGAGCACGCCAAGCTCTGGTTGACGAAGTTCAAGAACGAGCTCAGCGACGACGAGAAGTTCGTCTTACA
AGAGATAATAAAAATTAAGAGGAAACAGAACCCGCTCTACGGAGTGGTGCTGCCTTCGCCCAGCTCTTAA

Upstream 100 bases:

>100_bases
GCGCCGGCCTCGTACGGCCCTCCGAGAGAGCCGGTGGTCGCGGGTTCAAATCCCGCCGGCGGCTCCACATCTTAATCCCT
CTTCGCCCCGACTTGCAGAG

Downstream 100 bases:

>100_bases
ACGGTTCGTTTCTCCCTCTCCGGGATGAGGAGTAAGGGAGTCAGGAGATCTCGTTTAGAAATGACCGGAAACGGAACAGC
CGACCGGATGAGGAAGTTTG

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MLNLSAEQRPRLRQPIVAVLGHVDHGKTTLLDKIRGTVVALKEPGQITQHIGASLVPTDVIEKVTEPLKKIIPTVKLELP
GLLFIDTPGHEIFSNLRRRGGAVADLAILVVDLNEGFQPQTYEAVEILKQRRVPFVVAANKIDRIPGWRSYPDQPFLISY
RKQSEEVRERLDNKIYEIMGELAKLGFDSERFDRITNFTRQIAIVPISAKTGEGIPELLAVLAGLAQRYMKGRLKYVEGP
AKGVIMEVKEEPGYGSTIDTIIYDGIIRQGDTIVVGGIEGPIVTKVRALLVPAPLTEMRATKRFEPVEEVSAAAGVKIVA
PGLEKAVAGAPVYVVDSPEKLEELKEQVKREVEEVMIETDKEGVIVKADTLGTLEALVQFLKNRGIPVRMARVGPVTKRD
IVEAMTVKSKNKEYGVILAFNVKVLPEAKELAEKEGIKIFQHNVIYRLIEEFEEWLKALREEEKRKVLETLVRPGKIRIL
PGFVFRRSDPAIVGVEVLGGVIKPKYPLMKEDGSRVGSILQIQDKGQSVPEARAGQQVAISIKGRVMVGRHIHEGDVLYT
DVPAEHAKLWLTKFKNELSDDEKFVLQEIIKIKRKQNPLYGVVLPSPSS

Sequences:

>Translated_609_residues
MLNLSAEQRPRLRQPIVAVLGHVDHGKTTLLDKIRGTVVALKEPGQITQHIGASLVPTDVIEKVTEPLKKIIPTVKLELP
GLLFIDTPGHEIFSNLRRRGGAVADLAILVVDLNEGFQPQTYEAVEILKQRRVPFVVAANKIDRIPGWRSYPDQPFLISY
RKQSEEVRERLDNKIYEIMGELAKLGFDSERFDRITNFTRQIAIVPISAKTGEGIPELLAVLAGLAQRYMKGRLKYVEGP
AKGVIMEVKEEPGYGSTIDTIIYDGIIRQGDTIVVGGIEGPIVTKVRALLVPAPLTEMRATKRFEPVEEVSAAAGVKIVA
PGLEKAVAGAPVYVVDSPEKLEELKEQVKREVEEVMIETDKEGVIVKADTLGTLEALVQFLKNRGIPVRMARVGPVTKRD
IVEAMTVKSKNKEYGVILAFNVKVLPEAKELAEKEGIKIFQHNVIYRLIEEFEEWLKALREEEKRKVLETLVRPGKIRIL
PGFVFRRSDPAIVGVEVLGGVIKPKYPLMKEDGSRVGSILQIQDKGQSVPEARAGQQVAISIKGRVMVGRHIHEGDVLYT
DVPAEHAKLWLTKFKNELSDDEKFVLQEIIKIKRKQNPLYGVVLPSPSS
>Mature_609_residues
MLNLSAEQRPRLRQPIVAVLGHVDHGKTTLLDKIRGTVVALKEPGQITQHIGASLVPTDVIEKVTEPLKKIIPTVKLELP
GLLFIDTPGHEIFSNLRRRGGAVADLAILVVDLNEGFQPQTYEAVEILKQRRVPFVVAANKIDRIPGWRSYPDQPFLISY
RKQSEEVRERLDNKIYEIMGELAKLGFDSERFDRITNFTRQIAIVPISAKTGEGIPELLAVLAGLAQRYMKGRLKYVEGP
AKGVIMEVKEEPGYGSTIDTIIYDGIIRQGDTIVVGGIEGPIVTKVRALLVPAPLTEMRATKRFEPVEEVSAAAGVKIVA
PGLEKAVAGAPVYVVDSPEKLEELKEQVKREVEEVMIETDKEGVIVKADTLGTLEALVQFLKNRGIPVRMARVGPVTKRD
IVEAMTVKSKNKEYGVILAFNVKVLPEAKELAEKEGIKIFQHNVIYRLIEEFEEWLKALREEEKRKVLETLVRPGKIRIL
PGFVFRRSDPAIVGVEVLGGVIKPKYPLMKEDGSRVGSILQIQDKGQSVPEARAGQQVAISIKGRVMVGRHIHEGDVLYT
DVPAEHAKLWLTKFKNELSDDEKFVLQEIIKIKRKQNPLYGVVLPSPSS

Specific function: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI84043963, Length=593, Percent_Identity=37.2681281618887, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI53729339, Length=282, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI53729337, Length=282, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1789559, Length=522, Percent_Identity=29.3103448275862, Blast_Score=140, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI212656558, Length=590, Percent_Identity=36.9491525423729, Blast_Score=393, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71994658, Length=495, Percent_Identity=27.0707070707071, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319282, Length=567, Percent_Identity=41.9753086419753, Blast_Score=408, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324550, Length=587, Percent_Identity=27.93867120954, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24656849, Length=578, Percent_Identity=39.1003460207612, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI28572034, Length=548, Percent_Identity=29.1970802919708, Blast_Score=142, Evalue=8e-34,
Organism=Drosophila melanogaster, GI160714833, Length=88, Percent_Identity=50, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2P_IGNH4 (A8A8D3)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001434592.1
- ProteinModelPortal:   A8A8D3
- SMR:   A8A8D3
- STRING:   A8A8D3
- GeneID:   5562612
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_0001
- eggNOG:   arNOG04413
- HOGENOM:   HBG635083
- OMA:   SPLRIVP
- ProtClustDB:   PRK04004
- BioCyc:   IHOS453591:IGNI_0001-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00100_A
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004544
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00491
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67807; Mature: 67807

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLSAEQRPRLRQPIVAVLGHVDHGKTTLLDKIRGTVVALKEPGQITQHIGASLVPTDV
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCHHHHHCCCCCCCHHH
IEKVTEPLKKIIPTVKLELPGLLFIDTPGHEIFSNLRRRGGAVADLAILVVDLNEGFQPQ
HHHHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC
TYEAVEILKQRRVPFVVAANKIDRIPGWRSYPDQPFLISYRKQSEEVRERLDNKIYEIMG
HHHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
ELAKLGFDSERFDRITNFTRQIAIVPISAKTGEGIPELLAVLAGLAQRYMKGRLKYVEGP
HHHHCCCCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCC
AKGVIMEVKEEPGYGSTIDTIIYDGIIRQGDTIVVGGIEGPIVTKVRALLVPAPLTEMRA
CCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHH
TKRFEPVEEVSAAAGVKIVAPGLEKAVAGAPVYVVDSPEKLEELKEQVKREVEEVMIETD
HHCCCCHHHHHHHCCCEEECCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
KEGVIVKADTLGTLEALVQFLKNRGIPVRMARVGPVTKRDIVEAMTVKSKNKEYGVILAF
CCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEE
NVKVLPEAKELAEKEGIKIFQHNVIYRLIEEFEEWLKALREEEKRKVLETLVRPGKIRIL
EEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PGFVFRRSDPAIVGVEVLGGVIKPKYPLMKEDGSRVGSILQIQDKGQSVPEARAGQQVAI
CCEEEECCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEE
SIKGRVMVGRHIHEGDVLYTDVPAEHAKLWLTKFKNELSDDEKFVLQEIIKIKRKQNPLY
EEECEEEECCEECCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEE
GVVLPSPSS
EEEECCCCC
>Mature Secondary Structure
MLNLSAEQRPRLRQPIVAVLGHVDHGKTTLLDKIRGTVVALKEPGQITQHIGASLVPTDV
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCHHHHHCCCCCCCHHH
IEKVTEPLKKIIPTVKLELPGLLFIDTPGHEIFSNLRRRGGAVADLAILVVDLNEGFQPQ
HHHHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC
TYEAVEILKQRRVPFVVAANKIDRIPGWRSYPDQPFLISYRKQSEEVRERLDNKIYEIMG
HHHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
ELAKLGFDSERFDRITNFTRQIAIVPISAKTGEGIPELLAVLAGLAQRYMKGRLKYVEGP
HHHHCCCCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCC
AKGVIMEVKEEPGYGSTIDTIIYDGIIRQGDTIVVGGIEGPIVTKVRALLVPAPLTEMRA
CCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHH
TKRFEPVEEVSAAAGVKIVAPGLEKAVAGAPVYVVDSPEKLEELKEQVKREVEEVMIETD
HHCCCCHHHHHHHCCCEEECCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
KEGVIVKADTLGTLEALVQFLKNRGIPVRMARVGPVTKRDIVEAMTVKSKNKEYGVILAF
CCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEE
NVKVLPEAKELAEKEGIKIFQHNVIYRLIEEFEEWLKALREEEKRKVLETLVRPGKIRIL
EEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PGFVFRRSDPAIVGVEVLGGVIKPKYPLMKEDGSRVGSILQIQDKGQSVPEARAGQQVAI
CCEEEECCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEE
SIKGRVMVGRHIHEGDVLYTDVPAEHAKLWLTKFKNELSDDEKFVLQEIIKIKRKQNPLY
EEECEEEECCEECCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEE
GVVLPSPSS
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA