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The map label for this gene is ytwF [H]

Identifier: 154687152

GI number: 154687152

Start: 2849810

End: 2850121

Strand: Reverse

Name: ytwF [H]

Synonym: RBAM_027240

Alternate gene names: 154687152

Gene position: 2850121-2849810 (Counterclockwise)

Preceding gene: 154687153

Following gene: 154687151

Centisome position: 72.73

GC content: 45.19

Gene sequence:

>312_bases
TTGAAAATAAATGAAATCAGCACAGCCGCACTGGAAGAAAAATTAAAAGCGGGAGAAGAGCTGTATCTGATTGATGTCAG
AGAAGACGAAGAAGTTGCCGAAGGAATGATTCCGGAGGCCGTTCACATCCGCATGGGGGACATCCCTGAGAAGATGGATG
CGTTAGACAAGGACAAGGAATATGTATTTATCTGCCGTTCCGGAAAAAGAAGCATGAACGTTTGTATGTATTTACATGAG
CAGGGCTTCCGTACGGTTAATGTTGAAGGCGGCATGCTGGAGTGGGAAGGCGAAACAAAGCCGAAGAAATAA

Upstream 100 bases:

>100_bases
TCCTACAGAGAGGGGCTTTTTTATCAGGCCTCTATCATAGAAATTGCACCGCGGGCTTTGGTATACTTAACACCATAAAG
ATGAATGGGAGTGTCACACA

Downstream 100 bases:

>100_bases
CGCTTTGAAGTGAAAGAAGAAAAGGGACGTGCGTCTTTTTCTTCTTTTTTTATAAAAAAACGGGAGGCGAATCAGGTGAA
ACCGATGATCCGCGCGATGA

Product: YtwF

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 103; Mature: 103

Protein sequence:

>103_residues
MKINEISTAALEEKLKAGEELYLIDVREDEEVAEGMIPEAVHIRMGDIPEKMDALDKDKEYVFICRSGKRSMNVCMYLHE
QGFRTVNVEGGMLEWEGETKPKK

Sequences:

>Translated_103_residues
MKINEISTAALEEKLKAGEELYLIDVREDEEVAEGMIPEAVHIRMGDIPEKMDALDKDKEYVFICRSGKRSMNVCMYLHE
QGFRTVNVEGGMLEWEGETKPKK
>Mature_103_residues
MKINEISTAALEEKLKAGEELYLIDVREDEEVAEGMIPEAVHIRMGDIPEKMDALDKDKEYVFICRSGKRSMNVCMYLHE
QGFRTVNVEGGMLEWEGETKPKK

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11823; Mature: 11823

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
6.8 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
6.8 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINEISTAALEEKLKAGEELYLIDVREDEEVAEGMIPEAVHIRMGDIPEKMDALDKDKE
CCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCEEEEEECCCCHHHHHCCCCCC
YVFICRSGKRSMNVCMYLHEQGFRTVNVEGGMLEWEGETKPKK
EEEEECCCCCCCEEEEEEECCCCEEEECCCCEEEECCCCCCCC
>Mature Secondary Structure
MKINEISTAALEEKLKAGEELYLIDVREDEEVAEGMIPEAVHIRMGDIPEKMDALDKDKE
CCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCEEEEEECCCCHHHHHCCCCCC
YVFICRSGKRSMNVCMYLHEQGFRTVNVEGGMLEWEGETKPKK
EEEEECCCCCCCEEEEEEECCCCEEEECCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]