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The map label for this gene is leuS

Identifier: 154687153

GI number: 154687153

Start: 2850238

End: 2852652

Strand: Reverse

Name: leuS

Synonym: RBAM_027250

Alternate gene names: 154687153

Gene position: 2852652-2850238 (Counterclockwise)

Preceding gene: 154687154

Following gene: 154687152

Centisome position: 72.8

GC content: 50.52

Gene sequence:

>2415_bases
TTGAGTTTTCAGCATGAAAAAATAGAAAAGAAATGGCAGAATTACTGGTTGGATCATAAAACGTTCGCCGTCAGTGAAGA
GAATGACAAGCCGAAGTTTTACGCGCTTGACATGTTCCCGTATCCTTCAGGAGCCGGCCTTCATGTCGGACATCCGGAAG
GCTATACGGCAACCGATATCCTTTCGCGCATGAGACGCATGCAAGGATACAATGTGCTTCATCCGATGGGATGGGACGCG
TTCGGGCTTCCTGCCGAGCAATACGCGCTTGACACGGGGAATGATCCGGCCGAGTTCACAAAACAAAATATAGATAATTT
CCGCCGCCAGATTCAATCGCTCGGATTTTCATATGATTGGGATCGTGAAATCAATACGACTGATCCGGACTATTACAAGT
GGACGCAGTGGATCTTCACCAAGCTGTACGAAAAAGGGCTCGCTTACGTAGACGAAGTGCCGGTCAACTGGTGCCCGGCG
CTCGGAACGGTGCTTGCGAACGAAGAAGTCATTGACGGCAAAAGCGAGCGGGGCGGCCATCCGGTTGAACGCCGCCCGAT
GAGACAATGGATGCTGAAAATCACCGCTTATGCGGACAGGCTTCTTGAAGATTTAGAAGACCTTGACTGGCCGGAAAGCA
TTAAAGACATGCAGCGCAACTGGATCGGACGCTCAGAAGGCGCAAACGTGCATTTCGCGATCGACGGCACTGATGACACG
TTTACCGTGTTTACGACAAGACCGGACACGCTGTTCGGCGCGGCTTATGCCGTACTGGCTCCTGAGCATGAGCTTGTGGA
GGCCATCACGACGCCTGAACAAAAAGAAGCGGTAGACGCTTATGTTAAAGAAGTTCAGGCGAAAAGTGACCTGGAACGGA
CGGATCTTGCGAAAACAAAAACGGGCGTCTTCACGGGCGCATATGCCGTAAACCCTGCAAACGGGGAAAAGCTGCCGATC
TGGATAGCGGATTATGTCCTCGCCTCTTACGGAACAGGCGCTGTAATGGCCGTGCCGGCGCATGATGAACGTGATTTTGA
ATTCGCAAAAGTGTTCAGCCTGCCTGTCAAAGAAGTCGTAAAAGGCGGAAACACGGAAGAAGAAGCGTACACGGGGGACG
GAGAGCATGTCAATTCCGGCTTTCTGAACGGACTGAAAAAAGCGGAAGCGATTGAAAAAATGATCGCCTGGCTTGAAGAA
ACGAAAAACGGTGAGAAAAAAGTGACATACCGTCTGCGTGATTGGCTGTTCAGCCGCCAGCGCTATTGGGGCGAGCCGAT
TCCGGTTATTCATTGGGAAGACGGCACGTCAACAGCCGTTCCGGCGGAAGAGCTGCCGCTGATTCTGCCGAAAACCGATG
AGATCAAACCGAGCGGAACAGGTGAATCACCGCTTGCCAACATTAAAGAGTGGGTGGAAGTCACTGATCCTGAAACGGGC
AAAAAAGGCAGAAGAGAAACGAACACGATGCCGCAATGGGCGGGAAGCTGCTGGTATTTCCTCCGCTTTATCGACCCGAA
AAATCCGGATCAGCTCGCTTCACCTGAGAAACTGGATAAATGGCTGCCCGTTGATATTTACATCGGAGGAGCGGAGCATG
CCGTGCTTCACCTTCTGTACGCGCGTTTCTGGCATAAATTCCTCTACGATATCGGTGTCGTGCCGACGAAAGAACCGTTC
CAAAAGCTGTATAACCAAGGAATGATTCTCGGGGAAAATAATGAAAAAATGAGTAAATCCCGCGGAAACGTCGTCAATCC
GGATGAAATTGTGGCGTCTCACGGAGCAGATACGCTCCGCCTGTACGAAATGTTTATGGGGCCGCTTGATGCATCTATCG
CCTGGTCTGAATCAGGTCTTGACGGAGCGCGCCGCTTCCTTGACCGCGTATGGCGTTTGTTTATCGAGGAAAACGGCGAG
CTGACGGGCAAAGTGACAGAAGGCGCAGGCGAAACTCTTGAGCGCGTCTATCATGAAACGGTCATGAAAGTGACAGAAAA
CTTCGAAGCCCTTCGTTTTAACACGGGCATTTCTCAGCTGATGGTCTTCATTAATGAAGCTTACAAAGCGAATGAACTGC
CGAGAGAATATATCGAAGGTTTTGTGAAGCTGCTTTCTCCTATTGCGCCGCATTTGGCGGAAGAACTGTGGGAAAAACTC
GGCCACGAAGGTTCGATTGCATACGAGGCTTGGCCTGCTTATGACGAATCAAAACTGGTGGACGATGAAGTGGAAATCGT
CGTCCAGCTGAACGGAAAAGTAAAAGCGAAATTAATGGTTCCGGCTGATGCTGATAAAGCAGCGTTAGAACAGCTCGCAC
AAGCTGACGAAAAAGTAAAAGAGCAGCTTGAAGGAAAAACGATCCGAAAAATCATTGCCGTACCGGGCAAGCTTGTCAAT
ATCGTGGCAAACTGA

Upstream 100 bases:

>100_bases
GGGAGTTTTTTTAGTTTTAGAATAATCTGAATATAATCTTTCTCATCCCGTCAATACTTGGACTACATACGCCGTATTCA
ATCAGAAGGAGGTTTTTACG

Downstream 100 bases:

>100_bases
CCGGGCGAAGCCCCTCTCCTACAGAGAGGGGCTTTTTTATCAGGCCTCTATCATAGAAATTGCACCGCGGGCTTTGGTAT
ACTTAACACCATAAAGATGA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 804; Mature: 803

Protein sequence:

>804_residues
MSFQHEKIEKKWQNYWLDHKTFAVSEENDKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMRRMQGYNVLHPMGWDA
FGLPAEQYALDTGNDPAEFTKQNIDNFRRQIQSLGFSYDWDREINTTDPDYYKWTQWIFTKLYEKGLAYVDEVPVNWCPA
LGTVLANEEVIDGKSERGGHPVERRPMRQWMLKITAYADRLLEDLEDLDWPESIKDMQRNWIGRSEGANVHFAIDGTDDT
FTVFTTRPDTLFGAAYAVLAPEHELVEAITTPEQKEAVDAYVKEVQAKSDLERTDLAKTKTGVFTGAYAVNPANGEKLPI
WIADYVLASYGTGAVMAVPAHDERDFEFAKVFSLPVKEVVKGGNTEEEAYTGDGEHVNSGFLNGLKKAEAIEKMIAWLEE
TKNGEKKVTYRLRDWLFSRQRYWGEPIPVIHWEDGTSTAVPAEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETG
KKGRRETNTMPQWAGSCWYFLRFIDPKNPDQLASPEKLDKWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPF
QKLYNQGMILGENNEKMSKSRGNVVNPDEIVASHGADTLRLYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEENGE
LTGKVTEGAGETLERVYHETVMKVTENFEALRFNTGISQLMVFINEAYKANELPREYIEGFVKLLSPIAPHLAEELWEKL
GHEGSIAYEAWPAYDESKLVDDEVEIVVQLNGKVKAKLMVPADADKAALEQLAQADEKVKEQLEGKTIRKIIAVPGKLVN
IVAN

Sequences:

>Translated_804_residues
MSFQHEKIEKKWQNYWLDHKTFAVSEENDKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMRRMQGYNVLHPMGWDA
FGLPAEQYALDTGNDPAEFTKQNIDNFRRQIQSLGFSYDWDREINTTDPDYYKWTQWIFTKLYEKGLAYVDEVPVNWCPA
LGTVLANEEVIDGKSERGGHPVERRPMRQWMLKITAYADRLLEDLEDLDWPESIKDMQRNWIGRSEGANVHFAIDGTDDT
FTVFTTRPDTLFGAAYAVLAPEHELVEAITTPEQKEAVDAYVKEVQAKSDLERTDLAKTKTGVFTGAYAVNPANGEKLPI
WIADYVLASYGTGAVMAVPAHDERDFEFAKVFSLPVKEVVKGGNTEEEAYTGDGEHVNSGFLNGLKKAEAIEKMIAWLEE
TKNGEKKVTYRLRDWLFSRQRYWGEPIPVIHWEDGTSTAVPAEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETG
KKGRRETNTMPQWAGSCWYFLRFIDPKNPDQLASPEKLDKWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPF
QKLYNQGMILGENNEKMSKSRGNVVNPDEIVASHGADTLRLYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEENGE
LTGKVTEGAGETLERVYHETVMKVTENFEALRFNTGISQLMVFINEAYKANELPREYIEGFVKLLSPIAPHLAEELWEKL
GHEGSIAYEAWPAYDESKLVDDEVEIVVQLNGKVKAKLMVPADADKAALEQLAQADEKVKEQLEGKTIRKIIAVPGKLVN
IVAN
>Mature_803_residues
SFQHEKIEKKWQNYWLDHKTFAVSEENDKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRMRRMQGYNVLHPMGWDAF
GLPAEQYALDTGNDPAEFTKQNIDNFRRQIQSLGFSYDWDREINTTDPDYYKWTQWIFTKLYEKGLAYVDEVPVNWCPAL
GTVLANEEVIDGKSERGGHPVERRPMRQWMLKITAYADRLLEDLEDLDWPESIKDMQRNWIGRSEGANVHFAIDGTDDTF
TVFTTRPDTLFGAAYAVLAPEHELVEAITTPEQKEAVDAYVKEVQAKSDLERTDLAKTKTGVFTGAYAVNPANGEKLPIW
IADYVLASYGTGAVMAVPAHDERDFEFAKVFSLPVKEVVKGGNTEEEAYTGDGEHVNSGFLNGLKKAEAIEKMIAWLEET
KNGEKKVTYRLRDWLFSRQRYWGEPIPVIHWEDGTSTAVPAEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETGK
KGRRETNTMPQWAGSCWYFLRFIDPKNPDQLASPEKLDKWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQ
KLYNQGMILGENNEKMSKSRGNVVNPDEIVASHGADTLRLYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEENGEL
TGKVTEGAGETLERVYHETVMKVTENFEALRFNTGISQLMVFINEAYKANELPREYIEGFVKLLSPIAPHLAEELWEKLG
HEGSIAYEAWPAYDESKLVDDEVEIVVQLNGKVKAKLMVPADADKAALEQLAQADEKVKEQLEGKTIRKIIAVPGKLVNI
VAN

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=877, Percent_Identity=33.751425313569, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI94721239, Length=181, Percent_Identity=28.1767955801105, Blast_Score=74, Evalue=8e-13,
Organism=Homo sapiens, GI94721241, Length=181, Percent_Identity=28.1767955801105, Blast_Score=74, Evalue=8e-13,
Organism=Escherichia coli, GI1786861, Length=863, Percent_Identity=42.5260718424102, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=391, Percent_Identity=24.2966751918159, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71997510, Length=808, Percent_Identity=29.3316831683168, Blast_Score=317, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI71997517, Length=808, Percent_Identity=29.2079207920792, Blast_Score=317, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI212645227, Length=376, Percent_Identity=27.9255319148936, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17554638, Length=279, Percent_Identity=26.8817204301075, Blast_Score=75, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323414, Length=853, Percent_Identity=36.9284876905041, Blast_Score=477, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6321531, Length=433, Percent_Identity=23.3256351039261, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319395, Length=186, Percent_Identity=29.5698924731183, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355409, Length=776, Percent_Identity=32.4742268041237, Blast_Score=371, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281366294, Length=829, Percent_Identity=22.7985524728589, Blast_Score=107, Evalue=4e-23,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_BACA2 (A7Z7V7)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422314.1
- ProteinModelPortal:   A7Z7V7
- SMR:   A7Z7V7
- STRING:   A7Z7V7
- EnsemblBacteria:   EBBACT00000004370
- GeneID:   5462049
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_027250
- eggNOG:   COG0495
- GeneTree:   EBGT00070000031891
- HOGENOM:   HBG693845
- OMA:   ERGNFPV
- ProtClustDB:   PRK00390
- BioCyc:   BAMY326423:RBAM_027250-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 91271; Mature: 91140

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 579-579

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQHEKIEKKWQNYWLDHKTFAVSEENDKPKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCEECCCCCCCCHHHH
LSRMRRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTKQNIDNFRRQIQSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DREINTTDPDYYKWTQWIFTKLYEKGLAYVDEVPVNWCPALGTVLANEEVIDGKSERGGH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCCCC
PVERRPMRQWMLKITAYADRLLEDLEDLDWPESIKDMQRNWIGRSEGANVHFAIDGTDDT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
FTVFTTRPDTLFGAAYAVLAPEHELVEAITTPEQKEAVDAYVKEVQAKSDLERTDLAKTK
EEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TGVFTGAYAVNPANGEKLPIWIADYVLASYGTGAVMAVPAHDERDFEFAKVFSLPVKEVV
CCEEECEEEECCCCCCEEEEEHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCHHHHH
KGGNTEEEAYTGDGEHVNSGFLNGLKKAEAIEKMIAWLEETKNGEKKVTYRLRDWLFSRQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RYWGEPIPVIHWEDGTSTAVPAEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETG
HHCCCCCCEEEECCCCCCCCCHHHCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
KKGRRETNTMPQWAGSCWYFLRFIDPKNPDQLASPEKLDKWLPVDIYIGGAEHAVLHLLY
CCCCCCCCCCCHHHCCCEEEEEECCCCCCHHCCCHHHHHCCCCEEEEECCHHHHHHHHHH
ARFWHKFLYDIGVVPTKEPFQKLYNQGMILGENNEKMSKSRGNVVNPDEIVASHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEECCCCHHHHHCCCCCCCHHHHHHHCCCHHHH
LYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEENGELTGKVTEGAGETLERVYHET
HHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
VMKVTENFEALRFNTGISQLMVFINEAYKANELPREYIEGFVKLLSPIAPHLAEELWEKL
HHHHHHCCHHEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GHEGSIAYEAWPAYDESKLVDDEVEIVVQLNGKVKAKLMVPADADKAALEQLAQADEKVK
CCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHH
EQLEGKTIRKIIAVPGKLVNIVAN
HHHCCHHHHHHHHCCCHHHHEECC
>Mature Secondary Structure 
SFQHEKIEKKWQNYWLDHKTFAVSEENDKPKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCEECCCCCCCCHHHH
LSRMRRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTKQNIDNFRRQIQSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DREINTTDPDYYKWTQWIFTKLYEKGLAYVDEVPVNWCPALGTVLANEEVIDGKSERGGH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCCCC
PVERRPMRQWMLKITAYADRLLEDLEDLDWPESIKDMQRNWIGRSEGANVHFAIDGTDDT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
FTVFTTRPDTLFGAAYAVLAPEHELVEAITTPEQKEAVDAYVKEVQAKSDLERTDLAKTK
EEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TGVFTGAYAVNPANGEKLPIWIADYVLASYGTGAVMAVPAHDERDFEFAKVFSLPVKEVV
CCEEECEEEECCCCCCEEEEEHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCHHHHH
KGGNTEEEAYTGDGEHVNSGFLNGLKKAEAIEKMIAWLEETKNGEKKVTYRLRDWLFSRQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RYWGEPIPVIHWEDGTSTAVPAEELPLILPKTDEIKPSGTGESPLANIKEWVEVTDPETG
HHCCCCCCEEEECCCCCCCCCHHHCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
KKGRRETNTMPQWAGSCWYFLRFIDPKNPDQLASPEKLDKWLPVDIYIGGAEHAVLHLLY
CCCCCCCCCCCHHHCCCEEEEEECCCCCCHHCCCHHHHHCCCCEEEEECCHHHHHHHHHH
ARFWHKFLYDIGVVPTKEPFQKLYNQGMILGENNEKMSKSRGNVVNPDEIVASHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEECCCCHHHHHCCCCCCCHHHHHHHCCCHHHH
LYEMFMGPLDASIAWSESGLDGARRFLDRVWRLFIEENGELTGKVTEGAGETLERVYHET
HHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
VMKVTENFEALRFNTGISQLMVFINEAYKANELPREYIEGFVKLLSPIAPHLAEELWEKL
HHHHHHCCHHEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GHEGSIAYEAWPAYDESKLVDDEVEIVVQLNGKVKAKLMVPADADKAALEQLAQADEKVK
CCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHH
EQLEGKTIRKIIAVPGKLVNIVAN
HHHCCHHHHHHHHCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA