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The map label for this gene is trmB

Identifier: 154687129

GI number: 154687129

Start: 2826704

End: 2827357

Strand: Reverse

Name: trmB

Synonym: RBAM_027010

Alternate gene names: 154687129

Gene position: 2827357-2826704 (Counterclockwise)

Preceding gene: 154687131

Following gene: 154687128

Centisome position: 72.15

GC content: 46.18

Gene sequence:

>654_bases
ATGAGAATGCGCCATAAACCTTGGGCGAATGATTTTCTGGCAGAAAACGCTGACATCGCCATCAGTGATCCGGCCCAGTA
TAAAGGGCGGTGGAACACGGTCTTTGGCAATGACAACCCGATCCATATTGAAGTCGGTACGGGAAAAGGGCAGTTCATTT
CAGGAATGGCCAAGCAGAATCCGGATATCAACTATATCGGGATTGAGCTGTTTAAAAGCGTCATCGTCACTGCCGTCCAA
AAAGTAAAGGACAGCGGGGCTCACAATGTCCGTTTGTTAAATATAGACGCGGAAACACTGAATGACGTATTTGAACGGGG
AGAAATCAAGCGCGTATATCTAAACTTTTCTGACCCGTGGCCGAAAAACCGCCACGAAAAGCGCCGTTTAACATTTAAAA
CGTTCTTGAAAAAATACGAAGAAGTGATGGGCGAAACAGGTGAGATTCATTTTAAAACCGATAACCGCGGTTTATTCGAA
TACTCTCTGAAAAGCTTTTCCGAATACGGCCTGCTTCTGACGGAAGTCAGCCTTGATCTGCACAACAGCGATCCGAAGGG
CAATATTATGACAGAATACGAAGAAAAATTCTCCGCGCTCGGCCAGCCGATTTACCGGGCCGAAGTTGAATGGAGAACAA
AAGACAGACTCTGA

Upstream 100 bases:

>100_bases
CCTCTTTTTTTATGCAGCCGGCGCCTTTAAAAGGCGCTTGCGGCTCTTTATGTTTCATGGTATGTTGTTACTCGAAATTA
TAGATAGAGGTGTCAATCAT

Downstream 100 bases:

>100_bases
TGCGGGAGTCTGTTTTTTTATGCCGCCATTTGCTACCATTACATCAGGAGGGATCAAGATGGAGACATTGAATATTGGGA
ATATTTCATTAACATGGCTG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRMRHKPWANDFLAENADIAISDPAQYKGRWNTVFGNDNPIHIEVGTGKGQFISGMAKQNPDINYIGIELFKSVIVTAVQ
KVKDSGAHNVRLLNIDAETLNDVFERGEIKRVYLNFSDPWPKNRHEKRRLTFKTFLKKYEEVMGETGEIHFKTDNRGLFE
YSLKSFSEYGLLLTEVSLDLHNSDPKGNIMTEYEEKFSALGQPIYRAEVEWRTKDRL

Sequences:

>Translated_217_residues
MRMRHKPWANDFLAENADIAISDPAQYKGRWNTVFGNDNPIHIEVGTGKGQFISGMAKQNPDINYIGIELFKSVIVTAVQ
KVKDSGAHNVRLLNIDAETLNDVFERGEIKRVYLNFSDPWPKNRHEKRRLTFKTFLKKYEEVMGETGEIHFKTDNRGLFE
YSLKSFSEYGLLLTEVSLDLHNSDPKGNIMTEYEEKFSALGQPIYRAEVEWRTKDRL
>Mature_217_residues
MRMRHKPWANDFLAENADIAISDPAQYKGRWNTVFGNDNPIHIEVGTGKGQFISGMAKQNPDINYIGIELFKSVIVTAVQ
KVKDSGAHNVRLLNIDAETLNDVFERGEIKRVYLNFSDPWPKNRHEKRRLTFKTFLKKYEEVMGETGEIHFKTDNRGLFE
YSLKSFSEYGLLLTEVSLDLHNSDPKGNIMTEYEEKFSALGQPIYRAEVEWRTKDRL

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=174, Percent_Identity=26.4367816091954, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BACA2 (A7Z7T3)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422290.1
- ProteinModelPortal:   A7Z7T3
- SMR:   A7Z7T3
- STRING:   A7Z7T3
- EnsemblBacteria:   EBBACT00000004539
- GeneID:   5462310
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_027010
- eggNOG:   COG0220
- GeneTree:   EBGT00050000000917
- HOGENOM:   HBG304014
- OMA:   PTEYETK
- ProtClustDB:   PRK00121
- BioCyc:   BAMY326423:RBAM_027010-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25135; Mature: 25135

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMRHKPWANDFLAENADIAISDPAQYKGRWNTVFGNDNPIHIEVGTGKGQFISGMAKQN
CCCCCCCCCHHHHHCCCCEEEECCHHHCCCEEEECCCCCEEEEEEECCCCHHHCCHHHCC
PDINYIGIELFKSVIVTAVQKVKDSGAHNVRLLNIDAETLNDVFERGEIKRVYLNFSDPW
CCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHCCCEEEEEEECCCCC
PKNRHEKRRLTFKTFLKKYEEVMGETGEIHFKTDNRGLFEYSLKSFSEYGLLLTEVSLDL
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEEE
HNSDPKGNIMTEYEEKFSALGQPIYRAEVEWRTKDRL
CCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MRMRHKPWANDFLAENADIAISDPAQYKGRWNTVFGNDNPIHIEVGTGKGQFISGMAKQN
CCCCCCCCCHHHHHCCCCEEEECCHHHCCCEEEECCCCCEEEEEEECCCCHHHCCHHHCC
PDINYIGIELFKSVIVTAVQKVKDSGAHNVRLLNIDAETLNDVFERGEIKRVYLNFSDPW
CCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHCCCEEEEEEECCCCC
PKNRHEKRRLTFKTFLKKYEEVMGETGEIHFKTDNRGLFEYSLKSFSEYGLLLTEVSLDL
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEEE
HNSDPKGNIMTEYEEKFSALGQPIYRAEVEWRTKDRL
CCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA