BacMap

Definition	Bacillus amyloliquefaciens FZB42, complete genome.
Accession	NC_009725
Length	3,918,589

Click here to switch to the map view.

The map label for this gene is fabG [H]

Identifier: 154687063

GI number: 154687063

Start: 2763431

End: 2764198

Strand: Reverse

Name: fabG [H]

Synonym: RBAM_026350

Alternate gene names: 154687063

Gene position: 2764198-2763431 (Counterclockwise)

Preceding gene: 154687064

Following gene: 154687062

Centisome position: 70.54

GC content: 49.22

Gene sequence:

>768_bases
GTGCGACATGCTCTAATCACTGCCGGTACAAAGGGATTGGGCCGAAAAGTGACTGAAATGCTTCTGGAAAAAGGCTTTTC
TGTCACAGTGAATTACAGACAAGACGAAGAAGCGGTCGCCTGTCTGAAGGCGGATTATCCCGGGTGCCATGACAGATTGC
AATTCGTTAAGGGCGATGTAACCCGGAAAGAAGATCTCATGCGGATCGCCGATGCAGCCCTTCAGCGGTTTGGAAGGATT
GACATACTGATTAACAATGCCGGGCCGTACATATTTGAACGCAAAAAGCTTGCCGATTACTCCGATGACGAATGGTATGA
CATGCTTGAAGGGAATTTAAGCGCCGTTTTCCATCTGTTTAAAGCGGTTATCCCGGTCATGAGACAGCAGCGGTTCGGCC
GGATTATTACATACGGTTTTCAAGGAGCGGACCATGCCCCCGGCTGGCTTCACCGTTCCGCTTTCGGAGCGGCTAAAGTC
GGATTGGCTTCGTTAACGAAAACCATTGCGATTGAAGAAGCTGAATCAGGCATTACGGCCAATATGGTCTGCCCGGGTGA
TATTGTAGGAGATATGAAAGAAGCGTCTATAGAGGAAGCAAGACGGAAAATCGGCAAAGAAAAGACGCCGATCGGCAGGT
CCGGAACCGGAGAGGACATTGCGAGAATCATCGCCTTTTTATGTGAAGATGATTCCGATCTTGTAACCGGAACGGTCATT
GAAGCAACAGGCGGGCTGAATGTGATTAACAGGCATCAATCCCTGTAA

Upstream 100 bases:

>100_bases
AGTGCAAAAAAGTGATATTGCCGTTTGTTTATAGTAAAGTAGAGGGAGAAACGTCTTGATTTTATTGAAGCGGCAACGTT
GCCTGGGGAGGGTATTCAAT

Downstream 100 bases:

>100_bases
GTTTTTTCGTTTGTCAAAAAGGGAACTTGATAAATATTAATTTTTTATGAGGTGAAAACGATGAAAGTGACTTATCACGG
ACATTCTGTCATTCAAGTGG

Product: 3-ketoacyl-(acyl-carrier-protein) reductase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRHALITAGTKGLGRKVTEMLLEKGFSVTVNYRQDEEAVACLKADYPGCHDRLQFVKGDVTRKEDLMRIADAALQRFGRI
DILINNAGPYIFERKKLADYSDDEWYDMLEGNLSAVFHLFKAVIPVMRQQRFGRIITYGFQGADHAPGWLHRSAFGAAKV
GLASLTKTIAIEEAESGITANMVCPGDIVGDMKEASIEEARRKIGKEKTPIGRSGTGEDIARIIAFLCEDDSDLVTGTVI
EATGGLNVINRHQSL

Sequences:

>Translated_255_residues
MRHALITAGTKGLGRKVTEMLLEKGFSVTVNYRQDEEAVACLKADYPGCHDRLQFVKGDVTRKEDLMRIADAALQRFGRI
DILINNAGPYIFERKKLADYSDDEWYDMLEGNLSAVFHLFKAVIPVMRQQRFGRIITYGFQGADHAPGWLHRSAFGAAKV
GLASLTKTIAIEEAESGITANMVCPGDIVGDMKEASIEEARRKIGKEKTPIGRSGTGEDIARIIAFLCEDDSDLVTGTVI
EATGGLNVINRHQSL
>Mature_255_residues
MRHALITAGTKGLGRKVTEMLLEKGFSVTVNYRQDEEAVACLKADYPGCHDRLQFVKGDVTRKEDLMRIADAALQRFGRI
DILINNAGPYIFERKKLADYSDDEWYDMLEGNLSAVFHLFKAVIPVMRQQRFGRIITYGFQGADHAPGWLHRSAFGAAKV
GLASLTKTIAIEEAESGITANMVCPGDIVGDMKEASIEEARRKIGKEKTPIGRSGTGEDIARIIAFLCEDDSDLVTGTVI
EATGGLNVINRHQSL

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=260, Percent_Identity=28.4615384615385, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI32483357, Length=252, Percent_Identity=30.952380952381, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=30.6122448979592, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI66933014, Length=210, Percent_Identity=29.5238095238095, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI126723750, Length=260, Percent_Identity=30, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI59889578, Length=217, Percent_Identity=27.1889400921659, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI15277342, Length=253, Percent_Identity=29.2490118577075, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI5031737, Length=244, Percent_Identity=27.4590163934426, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4504505, Length=189, Percent_Identity=31.7460317460317, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787335, Length=246, Percent_Identity=35.3658536585366, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1788459, Length=249, Percent_Identity=28.5140562248996, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=27.3092369477912, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082100, Length=246, Percent_Identity=29.2682926829268, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787905, Length=261, Percent_Identity=27.2030651340996, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082160, Length=250, Percent_Identity=26.8, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI2367175, Length=249, Percent_Identity=25.3012048192771, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786812, Length=253, Percent_Identity=28.4584980237154, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1790717, Length=250, Percent_Identity=26, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789378, Length=247, Percent_Identity=25.5060728744939, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789057, Length=255, Percent_Identity=24.3137254901961, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=252, Percent_Identity=30.952380952381, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560676, Length=252, Percent_Identity=28.5714285714286, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=260, Percent_Identity=30, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=29.0566037735849, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560332, Length=262, Percent_Identity=29.7709923664122, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=259, Percent_Identity=26.6409266409266, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17538480, Length=260, Percent_Identity=29.2307692307692, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=29.277566539924, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17551412, Length=260, Percent_Identity=29.2307692307692, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=268, Percent_Identity=27.2388059701493, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=274, Percent_Identity=28.1021897810219, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=26.2096774193548, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=255, Percent_Identity=25.0980392156863, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17565030, Length=263, Percent_Identity=28.5171102661597, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=268, Percent_Identity=26.4925373134328, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=262, Percent_Identity=27.4809160305344, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193204405, Length=267, Percent_Identity=25.4681647940075, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=28.5171102661597, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=27.5092936802974, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=25.8555133079848, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=25.8426966292135, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17536025, Length=261, Percent_Identity=26.8199233716475, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=246, Percent_Identity=27.6422764227642, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=187, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=260, Percent_Identity=27.3076923076923, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=122, Percent_Identity=34.4262295081967, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324126, Length=251, Percent_Identity=27.0916334661355, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355319, Length=247, Percent_Identity=27.9352226720648, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24644339, Length=250, Percent_Identity=27.6, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI23397609, Length=249, Percent_Identity=29.3172690763052, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357041, Length=250, Percent_Identity=27.2, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI18859837, Length=251, Percent_Identity=27.8884462151394, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571526, Length=195, Percent_Identity=28.7179487179487, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=26.1044176706827, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 28091; Mature: 28091

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHALITAGTKGLGRKVTEMLLEKGFSVTVNYRQDEEAVACLKADYPGCHDRLQFVKGDV
CCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCC
TRKEDLMRIADAALQRFGRIDILINNAGPYIFERKKLADYSDDEWYDMLEGNLSAVFHLF
CHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHCCCCCCHHHHHHHCCHHHHHHHH
KAVIPVMRQQRFGRIITYGFQGADHAPGWLHRSAFGAAKVGLASLTKTIAIEEAESGITA
HHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
NMVCPGDIVGDMKEASIEEARRKIGKEKTPIGRSGTGEDIARIIAFLCEDDSDLVTGTVI
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEE
EATGGLNVINRHQSL
ECCCCCHHHHHCCCC
>Mature Secondary Structure
MRHALITAGTKGLGRKVTEMLLEKGFSVTVNYRQDEEAVACLKADYPGCHDRLQFVKGDV
CCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCC
TRKEDLMRIADAALQRFGRIDILINNAGPYIFERKKLADYSDDEWYDMLEGNLSAVFHLF
CHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHCCCCCCHHHHHHHCCHHHHHHHH
KAVIPVMRQQRFGRIITYGFQGADHAPGWLHRSAFGAAKVGLASLTKTIAIEEAESGITA
HHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
NMVCPGDIVGDMKEASIEEARRKIGKEKTPIGRSGTGEDIARIIAFLCEDDSDLVTGTVI
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEE
EATGGLNVINRHQSL
ECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]