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The map label for this gene is citZ [H]

Identifier: 154687046

GI number: 154687046

Start: 2743825

End: 2744943

Strand: Reverse

Name: citZ [H]

Synonym: RBAM_026180

Alternate gene names: 154687046

Gene position: 2744943-2743825 (Counterclockwise)

Preceding gene: 154687047

Following gene: 154687045

Centisome position: 70.05

GC content: 47.99

Gene sequence:

>1119_bases
ATGACAGCAACACGCGGTCTTGAAGGGGTTGTAGCAACAACATCATCTGTAAGCTCTATTATCGACGATACCCTTACATA
TGTAGGGTACGATATTGACGACTTGACAGAGAATGCAAGTTTTGAAGAAATCATTTATTTGCTGTGGCATCTGCGCCTGC
CGAATAAAACGGAACTTGCTGAACTGAAAAAACAGCTTGCGAAAGAAGCTGCCGTTCCACAAGAAATCATTGAGCACTTC
AAATCCTATCCCCTCAATAACGTACATCCAATGGCTGCGCTCCGGACGGCGATTTCATTACTCGGTCTGACTGACAGTGA
AGCCGATGTTATGAATCCGGAAGCGAACTACAGAAAAGCCATCCGTCTGCAGGCGAAAGTGCCGGGCATTGTCGCCGCTT
TCTCAAGAATCCGCAAAGGACTTGATCCTGTTGAACCGAAGGAAGAGTACGGAATCGCGGAGAACTTCCTTTACACCTTA
AACGGTGAAGAGCCTTCTCCGATCGAAGTGGAAGCGTTTAACAAAGCGCTCATTCTTCATGCGGATCATGAGCTGAACGC
ATCAACATTTACGGCGAGAGTATGTGTCGCCACCTTATCTGATATTTACTCAGGCATCACTGCCGCCATCGGCGCTTTAA
AAGGACCGCTTCACGGCGGAGCCAATGAAGCCGTCATGAAAATGCTGACAGAAATCGGCGAAGTGGAAAATGCCGAGCCT
TATATCCGCAGCAAAATGGAGAAAAAGGAAAAAGTAATGGGCTTCGGACACCGTGTGTATAAGCATGGAGACCCGCGCGC
CAAACACTTAAAAGAAATGAGCAAACGCCTGACGAATCTGACGGGTGAAAGCAAATGGTACGAGATGTCAATCCGTGTCG
AAGAAATCGTAACGTCGGAGAAAAAACTCCCGCCTAACGTTGATTTCTATTCCGCATCTGTATACCACAGTCTCGGCATT
GATCACGATCTGTTCACGCCGATCTTTGCGGTCAGCAGAATGTCAGGATGGATCGCCCATATTCTTGAACAGTACGATAA
CAACCGCCTCATCCGCCCGCGCGCTGATTACACGGGTCCGGATAAGCAGACTTTTGTTCCGATTGATGAAAGAGCCTGA

Upstream 100 bases:

>100_bases
TTAAAGGGGAAAGGGATTGTTTGCATGACTCATTTTAAGTAAGCCGTATGTTTTATATACTCTTTAAAGGGGAATTTTGA
TTGAAAAGGAGAGGTTGAAT

Downstream 100 bases:

>100_bases
TCAAACGGGAGGCTGGTGATTTTCATGTCGTTAGCCTCTTGTTAATCAAGCCCGGATTGGTTTACAATTTTGATAGAATC
TTGAGAAAAGTCATTTTATT

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKTELAELKKQLAKEAAVPQEIIEHF
KSYPLNNVHPMAALRTAISLLGLTDSEADVMNPEANYRKAIRLQAKVPGIVAAFSRIRKGLDPVEPKEEYGIAENFLYTL
NGEEPSPIEVEAFNKALILHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEIGEVENAEP
YIRSKMEKKEKVMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRVEEIVTSEKKLPPNVDFYSASVYHSLGI
DHDLFTPIFAVSRMSGWIAHILEQYDNNRLIRPRADYTGPDKQTFVPIDERA

Sequences:

>Translated_372_residues
MTATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKTELAELKKQLAKEAAVPQEIIEHF
KSYPLNNVHPMAALRTAISLLGLTDSEADVMNPEANYRKAIRLQAKVPGIVAAFSRIRKGLDPVEPKEEYGIAENFLYTL
NGEEPSPIEVEAFNKALILHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEIGEVENAEP
YIRSKMEKKEKVMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRVEEIVTSEKKLPPNVDFYSASVYHSLGI
DHDLFTPIFAVSRMSGWIAHILEQYDNNRLIRPRADYTGPDKQTFVPIDERA
>Mature_371_residues
TATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKTELAELKKQLAKEAAVPQEIIEHFK
SYPLNNVHPMAALRTAISLLGLTDSEADVMNPEANYRKAIRLQAKVPGIVAAFSRIRKGLDPVEPKEEYGIAENFLYTLN
GEEPSPIEVEAFNKALILHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEIGEVENAEPY
IRSKMEKKEKVMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRVEEIVTSEKKLPPNVDFYSASVYHSLGID
HDLFTPIFAVSRMSGWIAHILEQYDNNRLIRPRADYTGPDKQTFVPIDERA

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=377, Percent_Identity=27.5862068965517, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=38.3152173913043, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1786939, Length=377, Percent_Identity=34.7480106100796, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17555174, Length=372, Percent_Identity=26.8817204301075, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324328, Length=330, Percent_Identity=26.0606060606061, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319850, Length=330, Percent_Identity=24.5454545454545, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6325257, Length=180, Percent_Identity=28.8888888888889, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24640124, Length=376, Percent_Identity=26.5957446808511, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24640126, Length=376, Percent_Identity=26.5957446808511, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21356863, Length=375, Percent_Identity=25.6, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 41590; Mature: 41459

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKTELA
CCCCCCCCCCEECHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHH
ELKKQLAKEAAVPQEIIEHFKSYPLNNVHPMAALRTAISLLGLTDSEADVMNPEANYRKA
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
IRLQAKVPGIVAAFSRIRKGLDPVEPKEEYGIAENFLYTLNGEEPSPIEVEAFNKALILH
EEECCCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHEEEEECCCCCCCEEHEECCCEEEEE
ADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEIGEVENAEP
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCH
YIRSKMEKKEKVMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRVEEIVTSE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHC
KKLPPNVDFYSASVYHSLGIDHDLFTPIFAVSRMSGWIAHILEQYDNNRLIRPRADYTGP
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
DKQTFVPIDERA
CCCEECCCCCCC
>Mature Secondary Structure 
TATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKTELA
CCCCCCCCCEECHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHH
ELKKQLAKEAAVPQEIIEHFKSYPLNNVHPMAALRTAISLLGLTDSEADVMNPEANYRKA
HHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
IRLQAKVPGIVAAFSRIRKGLDPVEPKEEYGIAENFLYTLNGEEPSPIEVEAFNKALILH
EEECCCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHEEEEECCCCCCCEEHEECCCEEEEE
ADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEIGEVENAEP
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCH
YIRSKMEKKEKVMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRVEEIVTSE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHC
KKLPPNVDFYSASVYHSLGIDHDLFTPIFAVSRMSGWIAHILEQYDNNRLIRPRADYTGP
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
DKQTFVPIDERA
CCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]