Click here to switch to the map view.

The map label for this gene is polA [H]

Identifier: 154687041

GI number: 154687041

Start: 2735856

End: 2738495

Strand: Reverse

Name: polA [H]

Synonym: RBAM_026130

Alternate gene names: 154687041

Gene position: 2738495-2735856 (Counterclockwise)

Preceding gene: 154687042

Following gene: 154687040

Centisome position: 69.88

GC content: 47.12

Gene sequence:

>2640_bases
ATGACGGAACGAAAAAAATTAGTGCTTGTCGACGGAAACAGTCTGGCGTACCGAGCGTTTTTTGCATTGCCGCTTTTAAG
CAATGAAAAAGGCATCCATACAAATGCCGTATACGGGTTTGCGATGATTTTAATGAAAATGCTGGAAGATGAAAAGCCGA
CCCACATGCTGGTGGCTTTTGATGCGGGAAAAACCACTTTCCGCCACAGTACGTTTAAAGAATATAAAGGCGGAAGACAA
AAAACGCCGCCGGAGCTTTCAGAACAGATGCCGTTCATCCGCGAGCTCCTTGATGCCTACAAAATCAGCCGCTACGAGCT
TCCTGAATATGAAGCGGACGATATTATCGGCACCCTTGCCGTATCTGCTGAAAAAGAAGGTTTCGAAGTAAAGGTAGTGT
CGGGGGACAAGGACTTAACCCAGCTTGCAACCGGGAAAACGACCGTTGCCATTACGAGAAAAGGCATTACCGACGTGGAA
GATTACACGCCGGAGCACGTGCAAGAAAAGTACGGTCTGACCCCTTTGCAAATCATTGATATGAAGGGATTAATGGGGGA
TTCATCCGATAACATACCGGGCGTCCCCGGCGTCGGAGAAAAAACCGCGATTAAGCTTTTAAAGCAGTTCCATTCGGTTG
AGAAACTGCTTGATTCCATTGATGAAGTCAGCGGAAAAAAATTAAAAGAAAAGCTTGAAGAATATAAGGAACAAGCCCTG
ATGAGCAAAGAGCTGGCGACGATTATGACAAATGCGCCGATTGAAGTCAAGCCCGAAGATTTAGATTACAAAGGGTTTGA
CCGTGAACAAGTTGTTGCCATTTTTAAAGACCTCGGTTTTAATACGCTTCTTGAGCGGCTCGGTGAAGAAAGCGGCGAAA
CGGCGGAAGAAAATTTTGAAGACATCAATGTTGAAACGGCAGAAGAAATCACTGAAGATATGCTCGTTTCGCCGTCCGCT
TTGGTTGTTGAACAAATCGGTGATAACTATCACGAAGAACCGATCCTCGGCTTTTCCATCGTGAATGAAACAGGTCTTTA
TTATATTCCGAAAGAAATCGCCATTCAGTCTGAAACTTTTAAATCTTGGGCTGAAGATGAATCGATGAAAAAGTGGGTGT
TCGACAGCAAACGTTCCGTCGTCGCTTTGCGCTGGCAGGGAATTGAGCTGAAGGGAACGGAATTTGACGCGCTTCTTGCT
GCCTACATTATAAACCCGGGCAATTCGTACGACGATGTCGCGAGCGTAGCGAAAGAATACGGGCTGAATATCGTTTCAAA
AGATGAAGCGGTATACGGAAAAGGCGCAAAGCGCGCCGTTCCGGCTGAAAAAGAGCTTGCCGATCATCTCGCGCGGAAAG
CGTCTGCCGTTAACAGCCTCCGTGACAAACTGCTGCACGCGCTGGAAGAAAATAATCAGCTTGAATTATATGAAGATCTC
GAACTCCCGCTTGCGCTAATTCTCGGAGAAATGGAATCAATGGGCGTCAAAGTGGATGTTGAAAGACTGAAGCGAATGGG
CGAAGAGCTCAAGGCAAAGCTGAAAGAATATGAAGAAAAAATCCATGACATCGCCGGTGAGCCGTTTAACATCAATTCAC
CAAAACAGCTCGGCGTCATTCTGTTTGAAAAGCTCGGCCTGCCGCCAGTAAAGAAAACAAAAACAGGCTATTCCACTTCA
GCTGATGTGCTTGAGAAGCTCGCAGACAAACATGATATCGTCGCGCACATTCTGCAATACAGACAAATCGGCAAACTCCA
ATCGACTTATATTGAAGGGCTGCTGAAAGTCACGCGCAAGGATTCTCATAAAGTGCATACACGATTCAATCAGGCATTGA
CTCAAACCGGACGCCTCAGCTCAACGGATCCGAACCTGCAAAATATTCCGATCCGGCTGGAGGAAGGGCGGAAAATCCGT
CAGGCTTTCGTACCGTCCGAACCGGACTGGCTTATTTTCGCCGCCGATTATTCACAAATTGAATTAAGGGTGCTTGCGCA
TATTTCAAAAGATGAAAATCTGATCGAAGCGTTTACGAATGACATGGACATTCACACAAAAACCGCCATGGACGTATTTC
ATGTCAGTGAAGATGAAGTGACACAAGCGATGCGCCGCCAGGCAAAGGCCGTCAATTTCGGCATTGTTTACGGCATCAGT
GATTACGGGCTGTCACAAAACCTCGGCATCACCCGGAAAGAAGCGGGCGCTTTTATAGACCGCTATTTGGAGAGTTTCCC
GGGCGTCAAGGCGTATATGGAAGAATCGGTGCAGGAAGCGAAGCAAAAAGGATATGTCACGACACTTATGCAGCGCCGCC
GCTATATTCCTGAGCTGACCAGCCGCAACTTCAACCTGCGGAGCTTTGCGGAACGGACGGCCATGAATACGCCGATTCAG
GGAAGCGCCGCCGATATTATTAAAAAAGCCATGATTGACATGGCAGCTGAGCTGAAAGAAAAAAACCTGAAGGCGCGACT
GCTGCTTCAGGTGCACGATGAATTGATTTTTGAAGCGCCTAAGGAAGAAATTGAAATATTAGAGAAGCTGGTTCCTGAAG
TGATGGAGCATGCTTTGGCATTAGACGTGCCGCTGAAGGCGGATTATGCTTCAGGGCCGTCTTGGTACGATGCAAAATAA

Upstream 100 bases:

>100_bases
TCGCTCATTTTCCTTTCAAATTGAACTTTGTTAAAATAAGAGAAGCTGTGAGATTAAAAAATAGAGGAATGACGAGCTTT
TGCTCTTAGGAGGATATTGA

Downstream 100 bases:

>100_bases
ACAGAGAAAAAGGAAGTGATGATTGTGCCGGAATTACCAGAAGTTGAAACGGTCCGGCGCACTCTGACCGGTCTTGTGAA
GGGGAAAACGATTGAATCGG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 879; Mature: 878

Protein sequence:

>879_residues
MTERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFAMILMKMLEDEKPTHMLVAFDAGKTTFRHSTFKEYKGGRQ
KTPPELSEQMPFIRELLDAYKISRYELPEYEADDIIGTLAVSAEKEGFEVKVVSGDKDLTQLATGKTTVAITRKGITDVE
DYTPEHVQEKYGLTPLQIIDMKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHSVEKLLDSIDEVSGKKLKEKLEEYKEQAL
MSKELATIMTNAPIEVKPEDLDYKGFDREQVVAIFKDLGFNTLLERLGEESGETAEENFEDINVETAEEITEDMLVSPSA
LVVEQIGDNYHEEPILGFSIVNETGLYYIPKEIAIQSETFKSWAEDESMKKWVFDSKRSVVALRWQGIELKGTEFDALLA
AYIINPGNSYDDVASVAKEYGLNIVSKDEAVYGKGAKRAVPAEKELADHLARKASAVNSLRDKLLHALEENNQLELYEDL
ELPLALILGEMESMGVKVDVERLKRMGEELKAKLKEYEEKIHDIAGEPFNINSPKQLGVILFEKLGLPPVKKTKTGYSTS
ADVLEKLADKHDIVAHILQYRQIGKLQSTYIEGLLKVTRKDSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIR
QAFVPSEPDWLIFAADYSQIELRVLAHISKDENLIEAFTNDMDIHTKTAMDVFHVSEDEVTQAMRRQAKAVNFGIVYGIS
DYGLSQNLGITRKEAGAFIDRYLESFPGVKAYMEESVQEAKQKGYVTTLMQRRRYIPELTSRNFNLRSFAERTAMNTPIQ
GSAADIIKKAMIDMAAELKEKNLKARLLLQVHDELIFEAPKEEIEILEKLVPEVMEHALALDVPLKADYASGPSWYDAK

Sequences:

>Translated_879_residues
MTERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFAMILMKMLEDEKPTHMLVAFDAGKTTFRHSTFKEYKGGRQ
KTPPELSEQMPFIRELLDAYKISRYELPEYEADDIIGTLAVSAEKEGFEVKVVSGDKDLTQLATGKTTVAITRKGITDVE
DYTPEHVQEKYGLTPLQIIDMKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHSVEKLLDSIDEVSGKKLKEKLEEYKEQAL
MSKELATIMTNAPIEVKPEDLDYKGFDREQVVAIFKDLGFNTLLERLGEESGETAEENFEDINVETAEEITEDMLVSPSA
LVVEQIGDNYHEEPILGFSIVNETGLYYIPKEIAIQSETFKSWAEDESMKKWVFDSKRSVVALRWQGIELKGTEFDALLA
AYIINPGNSYDDVASVAKEYGLNIVSKDEAVYGKGAKRAVPAEKELADHLARKASAVNSLRDKLLHALEENNQLELYEDL
ELPLALILGEMESMGVKVDVERLKRMGEELKAKLKEYEEKIHDIAGEPFNINSPKQLGVILFEKLGLPPVKKTKTGYSTS
ADVLEKLADKHDIVAHILQYRQIGKLQSTYIEGLLKVTRKDSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIR
QAFVPSEPDWLIFAADYSQIELRVLAHISKDENLIEAFTNDMDIHTKTAMDVFHVSEDEVTQAMRRQAKAVNFGIVYGIS
DYGLSQNLGITRKEAGAFIDRYLESFPGVKAYMEESVQEAKQKGYVTTLMQRRRYIPELTSRNFNLRSFAERTAMNTPIQ
GSAADIIKKAMIDMAAELKEKNLKARLLLQVHDELIFEAPKEEIEILEKLVPEVMEHALALDVPLKADYASGPSWYDAK
>Mature_878_residues
TERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFAMILMKMLEDEKPTHMLVAFDAGKTTFRHSTFKEYKGGRQK
TPPELSEQMPFIRELLDAYKISRYELPEYEADDIIGTLAVSAEKEGFEVKVVSGDKDLTQLATGKTTVAITRKGITDVED
YTPEHVQEKYGLTPLQIIDMKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHSVEKLLDSIDEVSGKKLKEKLEEYKEQALM
SKELATIMTNAPIEVKPEDLDYKGFDREQVVAIFKDLGFNTLLERLGEESGETAEENFEDINVETAEEITEDMLVSPSAL
VVEQIGDNYHEEPILGFSIVNETGLYYIPKEIAIQSETFKSWAEDESMKKWVFDSKRSVVALRWQGIELKGTEFDALLAA
YIINPGNSYDDVASVAKEYGLNIVSKDEAVYGKGAKRAVPAEKELADHLARKASAVNSLRDKLLHALEENNQLELYEDLE
LPLALILGEMESMGVKVDVERLKRMGEELKAKLKEYEEKIHDIAGEPFNINSPKQLGVILFEKLGLPPVKKTKTGYSTSA
DVLEKLADKHDIVAHILQYRQIGKLQSTYIEGLLKVTRKDSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIRQ
AFVPSEPDWLIFAADYSQIELRVLAHISKDENLIEAFTNDMDIHTKTAMDVFHVSEDEVTQAMRRQAKAVNFGIVYGISD
YGLSQNLGITRKEAGAFIDRYLESFPGVKAYMEESVQEAKQKGYVTTLMQRRRYIPELTSRNFNLRSFAERTAMNTPIQG
SAADIIKKAMIDMAAELKEKNLKARLLLQVHDELIFEAPKEEIEILEKLVPEVMEHALALDVPLKADYASGPSWYDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=515, Percent_Identity=34.1747572815534, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI139394648, Length=550, Percent_Identity=26.7272727272727, Blast_Score=205, Evalue=2e-52,
Organism=Escherichia coli, GI1790294, Length=933, Percent_Identity=39.4426580921758, Blast_Score=577, Evalue=1e-165,
Organism=Escherichia coli, GI226510970, Length=220, Percent_Identity=30.9090909090909, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI86563272, Length=421, Percent_Identity=30.8788598574822, Blast_Score=191, Evalue=2e-48,
Organism=Drosophila melanogaster, GI17933644, Length=426, Percent_Identity=33.5680751173709, Blast_Score=234, Evalue=2e-61,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 99042; Mature: 98911

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFAMILMKMLEDEKPTHMLVAF
CCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEE
DAGKTTFRHSTFKEYKGGRQKTPPELSEQMPFIRELLDAYKISRYELPEYEADDIIGTLA
ECCCCHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHE
VSAEKEGFEVKVVSGDKDLTQLATGKTTVAITRKGITDVEDYTPEHVQEKYGLTPLQIID
EECCCCCCEEEEECCCCHHHHHHCCCEEEEEEECCCCCCHHCCHHHHHHHHCCCCEEEEE
MKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHSVEKLLDSIDEVSGKKLKEKLEEYKEQAL
CHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
MSKELATIMTNAPIEVKPEDLDYKGFDREQVVAIFKDLGFNTLLERLGEESGETAEENFE
HHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHH
DINVETAEEITEDMLVSPSALVVEQIGDNYHEEPILGFSIVNETGLYYIPKEIAIQSETF
HCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCEEECHHHHHCHHHH
KSWAEDESMKKWVFDSKRSVVALRWQGIELKGTEFDALLAAYIINPGNSYDDVASVAKEY
HHHHHHHHHHHHHHCCCCCEEEEEEECEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
GLNIVSKDEAVYGKGAKRAVPAEKELADHLARKASAVNSLRDKLLHALEENNQLELYEDL
CCCEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
ELPLALILGEMESMGVKVDVERLKRMGEELKAKLKEYEEKIHDIAGEPFNINSPKQLGVI
CCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LFEKLGLPPVKKTKTGYSTSADVLEKLADKHDIVAHILQYRQIGKLQSTYIEGLLKVTRK
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCEEEEECCCCHH
ELRVLAHISKDENLIEAFTNDMDIHTKTAMDVFHVSEDEVTQAMRRQAKAVNFGIVYGIS
HHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEC
DYGLSQNLGITRKEAGAFIDRYLESFPGVKAYMEESVQEAKQKGYVTTLMQRRRYIPELT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHH
SRNFNLRSFAERTAMNTPIQGSAADIIKKAMIDMAAELKEKNLKARLLLQVHDELIFEAP
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
KEEIEILEKLVPEVMEHALALDVPLKADYASGPSWYDAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFAMILMKMLEDEKPTHMLVAF
CCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEE
DAGKTTFRHSTFKEYKGGRQKTPPELSEQMPFIRELLDAYKISRYELPEYEADDIIGTLA
ECCCCHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHE
VSAEKEGFEVKVVSGDKDLTQLATGKTTVAITRKGITDVEDYTPEHVQEKYGLTPLQIID
EECCCCCCEEEEECCCCHHHHHHCCCEEEEEEECCCCCCHHCCHHHHHHHHCCCCEEEEE
MKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHSVEKLLDSIDEVSGKKLKEKLEEYKEQAL
CHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
MSKELATIMTNAPIEVKPEDLDYKGFDREQVVAIFKDLGFNTLLERLGEESGETAEENFE
HHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHH
DINVETAEEITEDMLVSPSALVVEQIGDNYHEEPILGFSIVNETGLYYIPKEIAIQSETF
HCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCEEECHHHHHCHHHH
KSWAEDESMKKWVFDSKRSVVALRWQGIELKGTEFDALLAAYIINPGNSYDDVASVAKEY
HHHHHHHHHHHHHHCCCCCEEEEEEECEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
GLNIVSKDEAVYGKGAKRAVPAEKELADHLARKASAVNSLRDKLLHALEENNQLELYEDL
CCCEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
ELPLALILGEMESMGVKVDVERLKRMGEELKAKLKEYEEKIHDIAGEPFNINSPKQLGVI
CCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LFEKLGLPPVKKTKTGYSTSADVLEKLADKHDIVAHILQYRQIGKLQSTYIEGLLKVTRK
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCEEEEECCCCHH
ELRVLAHISKDENLIEAFTNDMDIHTKTAMDVFHVSEDEVTQAMRRQAKAVNFGIVYGIS
HHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEC
DYGLSQNLGITRKEAGAFIDRYLESFPGVKAYMEESVQEAKQKGYVTTLMQRRRYIPELT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHH
SRNFNLRSFAERTAMNTPIQGSAADIIKKAMIDMAAELKEKNLKARLLLQVHDELIFEAP
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
KEEIEILEKLVPEVMEHALALDVPLKADYASGPSWYDAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]