Click here to switch to the map view.

The map label for this gene is thrS [H]

Identifier: 154687027

GI number: 154687027

Start: 2721966

End: 2723897

Strand: Reverse

Name: thrS [H]

Synonym: RBAM_025990

Alternate gene names: 154687027

Gene position: 2723897-2721966 (Counterclockwise)

Preceding gene: 154687028

Following gene: 154687025

Centisome position: 69.51

GC content: 49.22

Gene sequence:

>1932_bases
ATGTCAGATATGATCAACATCACATTTCCGGACGGAGCGGTAAAGGAATTTCCGAAAGGAACAACAACAGAAGATATCGC
GGCATCCATCAGTCCGGGATTAAAGAAAAAGTCTTTAGCCGGAAAACTGAACGGACAGGAAATTGATTTAAGAACACCGA
TTCAAGAGGATGGTGCCGTAGAAATCATTACAGAAGGCACAGAAGAAGGTTTGAACATCATGCGGCACAGCACGGCGCAT
TTATTGGCCCAGGCGATTAAACGCATTTATAAAGATGTGAAATTCGGTGTCGGTCCCGTCATTGAAAACGGGTTCTATTA
TGACGTTGAAATGGAGCAGGCGCTGACGCCGGATGACCTTCCGAAGATTGAAAAAGAAATGAAACGCATCGTCGGTGAAA
ATCTGCCGATTATCCGAAAAGAAGTGAGCCGTGATGAAGCAAAAGCCATGTTTGCGGAAATCGGCGATAATCTGAAGCTT
GAACTGCTGGATGCCATCCCTGAAGACGAAACGGTCTCCATTTATGAGCAGGGCGAATTTTTTGACCTGTGCCGCGGCGT
GCACGTGCCGTCTACAGGCAAAATTAAAGAATTTAAGCTGCTTAGCCTTGCGGGCGCATACTGGCGCGGCGACAGCAAAA
ACCAAATGCTTCAGCGCATTTACGGCACGGCCTTCTTTAAAAAGGATGACTTGAAAGAGCATTTGCGGATGCTTGAAGAA
GCGAAAGAGCGTGACCACCGAAAACTGGGCAAAGAATTAAAATTGTTCGCCAACTCTCAAAAAGTCGGACAAGGCCTGCC
GCTCTGGCTGCCGAAAGGCGCAACCATCCGACGCGTGATCGAGCGCTACATTGTGGATAAAGAGCTGAGCCTCGGTTATG
AGCACGTCTATACGCCTGTTCTCGGAAGCAAAGACCTGTACGAAACATCAGGGCACTGGGATCATTATCAGGATACGATG
TTCCCGCCGATGGAAATGGACAATGAAACACTCGTGCTCCGTCCGATGAACTGTCCTCACCATATGATGATTTACAAACA
GGACATTCACAGCTACCGCGAGCTTCCGATCCGCATCGCAGAGCTGGGAACGATGCATCGTTATGAAATGTCCGGGGCGC
TTTCAGGTCTTCAGCGCGTACGCGGCATGACGTTAAATGACGCGCACATTTTCGTCCGCCCTGACCAGATTAAAGAAGAA
TTCATCCGCACGGTCCGTTTGATTCAAGACGTATACGAAGACTTCGGCCTGAATGATTACACATTCCGCCTGTCTTACCG
CGATCCTGAAGATACGGAAAAATATTTTGATGATGATGAGATGTGGAACAAAGCGCAGTCCATGCTGAAAGCGGCAATGG
ATGAGATGGGGCACGACTACTATGAAGCGGAAGGCGAAGCGGCGTTTTACGGCCCTAAGCTTGACGTGCAGGTGAAAACG
GCGATCGGCAAAGAAGAGACATTGTCAACTGTACAGCTTGACTTCCTTCTTCCGGAACGCTTTGATCTCACTTACGTCGG
AGAAGACGGCAAACAGCACCGTCCTGTCGTTATTCACCGCGGCGTCGTGTCAACGATGGAGCGTTTCGTTGCTTTCTTAA
TCGAAGAGCACAAAGGCGCCCTTCCGACATGGCTTGCGCCGGTGCAATTCCAAGTCATTCCTGTATCACCGTCCGTGCAT
CTTGATTATGCGAAAAAAGTGCAGGAGCGCCTGCAGCGTGAAGGCCTGCGTGTCGAGCTTGACAGCCGGGATGAGAAAAT
CGGCTATAAAATCCGTGAAGCGCAAATGCAAAAAATCCCGTACATGCTTGTGGTCGGTGACCAAGAAGCGGAAAACGGCG
CGGTAAACGTCCGGAAATACGGCGAGCAGAAATCAGAAACCGTCTCATTGGACGATTTTGTGAAAAAGGCTGTAGCCGAA
GCGAAAAAATAA

Upstream 100 bases:

>100_bases
CGCGCGGAAAAAAGGGTGGAACCACGATTCCGTTTATTTAACCTCGTCCCTTTCATAGGGGGCGGGGTTTTTGATTTGCA
TAAAAAAAGGAGTGACGAAG

Downstream 100 bases:

>100_bases
CCGGAAATACAAAAAACCCCGTTCATTCAGAATGGGGTTTTTTTGCGTATAAAATTATCTTTCTTTTTGTTTCTCTATAA
TCGCAAACAGCTCGTGAAGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MSDMINITFPDGAVKEFPKGTTTEDIAASISPGLKKKSLAGKLNGQEIDLRTPIQEDGAVEIITEGTEEGLNIMRHSTAH
LLAQAIKRIYKDVKFGVGPVIENGFYYDVEMEQALTPDDLPKIEKEMKRIVGENLPIIRKEVSRDEAKAMFAEIGDNLKL
ELLDAIPEDETVSIYEQGEFFDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRIYGTAFFKKDDLKEHLRMLEE
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELSLGYEHVYTPVLGSKDLYETSGHWDHYQDTM
FPPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKEE
FIRTVRLIQDVYEDFGLNDYTFRLSYRDPEDTEKYFDDDEMWNKAQSMLKAAMDEMGHDYYEAEGEAAFYGPKLDVQVKT
AIGKEETLSTVQLDFLLPERFDLTYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGALPTWLAPVQFQVIPVSPSVH
LDYAKKVQERLQREGLRVELDSRDEKIGYKIREAQMQKIPYMLVVGDQEAENGAVNVRKYGEQKSETVSLDDFVKKAVAE
AKK

Sequences:

>Translated_643_residues
MSDMINITFPDGAVKEFPKGTTTEDIAASISPGLKKKSLAGKLNGQEIDLRTPIQEDGAVEIITEGTEEGLNIMRHSTAH
LLAQAIKRIYKDVKFGVGPVIENGFYYDVEMEQALTPDDLPKIEKEMKRIVGENLPIIRKEVSRDEAKAMFAEIGDNLKL
ELLDAIPEDETVSIYEQGEFFDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRIYGTAFFKKDDLKEHLRMLEE
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELSLGYEHVYTPVLGSKDLYETSGHWDHYQDTM
FPPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKEE
FIRTVRLIQDVYEDFGLNDYTFRLSYRDPEDTEKYFDDDEMWNKAQSMLKAAMDEMGHDYYEAEGEAAFYGPKLDVQVKT
AIGKEETLSTVQLDFLLPERFDLTYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGALPTWLAPVQFQVIPVSPSVH
LDYAKKVQERLQREGLRVELDSRDEKIGYKIREAQMQKIPYMLVVGDQEAENGAVNVRKYGEQKSETVSLDDFVKKAVAE
AKK
>Mature_642_residues
SDMINITFPDGAVKEFPKGTTTEDIAASISPGLKKKSLAGKLNGQEIDLRTPIQEDGAVEIITEGTEEGLNIMRHSTAHL
LAQAIKRIYKDVKFGVGPVIENGFYYDVEMEQALTPDDLPKIEKEMKRIVGENLPIIRKEVSRDEAKAMFAEIGDNLKLE
LLDAIPEDETVSIYEQGEFFDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRIYGTAFFKKDDLKEHLRMLEEA
KERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELSLGYEHVYTPVLGSKDLYETSGHWDHYQDTMF
PPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKEEF
IRTVRLIQDVYEDFGLNDYTFRLSYRDPEDTEKYFDDDEMWNKAQSMLKAAMDEMGHDYYEAEGEAAFYGPKLDVQVKTA
IGKEETLSTVQLDFLLPERFDLTYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGALPTWLAPVQFQVIPVSPSVHL
DYAKKVQERLQREGLRVELDSRDEKIGYKIREAQMQKIPYMLVVGDQEAENGAVNVRKYGEQKSETVSLDDFVKKAVAEA
KK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=637, Percent_Identity=38.1475667189953, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI38570111, Length=621, Percent_Identity=38.3252818035427, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI20070344, Length=654, Percent_Identity=34.4036697247706, Blast_Score=374, Evalue=1e-103,
Organism=Escherichia coli, GI1788013, Length=637, Percent_Identity=42.5431711145997, Blast_Score=546, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71984184, Length=639, Percent_Identity=37.8716744913928, Blast_Score=440, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71984192, Length=639, Percent_Identity=37.8716744913928, Blast_Score=440, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322112, Length=656, Percent_Identity=35.6707317073171, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6322654, Length=392, Percent_Identity=35.969387755102, Blast_Score=265, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24583839, Length=635, Percent_Identity=36.3779527559055, Blast_Score=431, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24583842, Length=635, Percent_Identity=36.3779527559055, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI28574141, Length=635, Percent_Identity=36.3779527559055, Blast_Score=430, Evalue=1e-120,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73745; Mature: 73614

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMINITFPDGAVKEFPKGTTTEDIAASISPGLKKKSLAGKLNGQEIDLRTPIQEDGAV
CCCCEEEECCCCHHHHCCCCCCHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCCCE
EIITEGTEEGLNIMRHSTAHLLAQAIKRIYKDVKFGVGPVIENGFYYDVEMEQALTPDDL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEHHHCCCCCCC
PKIEKEMKRIVGENLPIIRKEVSRDEAKAMFAEIGDNLKLELLDAIPEDETVSIYEQGEF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCEEEEEECCCH
FDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRIYGTAFFKKDDLKEHLRMLEE
HHHHCCCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELSLGYEHVYTPV
HHHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCHHHHHHHHHHHCCHHHHCCHHHHHCCC
LGSKDLYETSGHWDHYQDTMFPPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHEEHHHHHHHHHCCEEEE
ELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKEEFIRTVRLIQDVYEDFGLNDY
HHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCE
TFRLSYRDPEDTEKYFDDDEMWNKAQSMLKAAMDEMGHDYYEAEGEAAFYGPKLDVQVKT
EEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCEEEEE
AIGKEETLSTVQLDFLLPERFDLTYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGA
CCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC
LPTWLAPVQFQVIPVSPSVHLDYAKKVQERLQREGLRVELDSRDEKIGYKIREAQMQKIP
CCCEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHCHHHHHHHHHHCC
YMLVVGDQEAENGAVNVRKYGEQKSETVSLDDFVKKAVAEAKK
EEEEECCCCCCCCCEEHHHHCCCCCCEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDMINITFPDGAVKEFPKGTTTEDIAASISPGLKKKSLAGKLNGQEIDLRTPIQEDGAV
CCCEEEECCCCHHHHCCCCCCHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCCCE
EIITEGTEEGLNIMRHSTAHLLAQAIKRIYKDVKFGVGPVIENGFYYDVEMEQALTPDDL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEHHHCCCCCCC
PKIEKEMKRIVGENLPIIRKEVSRDEAKAMFAEIGDNLKLELLDAIPEDETVSIYEQGEF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCEEEEEECCCH
FDLCRGVHVPSTGKIKEFKLLSLAGAYWRGDSKNQMLQRIYGTAFFKKDDLKEHLRMLEE
HHHHCCCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AKERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKELSLGYEHVYTPV
HHHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCHHHHHHHHHHHCCHHHHCCHHHHHCCC
LGSKDLYETSGHWDHYQDTMFPPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHEEHHHHHHHHHCCEEEE
ELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKEEFIRTVRLIQDVYEDFGLNDY
HHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCE
TFRLSYRDPEDTEKYFDDDEMWNKAQSMLKAAMDEMGHDYYEAEGEAAFYGPKLDVQVKT
EEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCEEEEE
AIGKEETLSTVQLDFLLPERFDLTYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEHKGA
CCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC
LPTWLAPVQFQVIPVSPSVHLDYAKKVQERLQREGLRVELDSRDEKIGYKIREAQMQKIP
CCCEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHCHHHHHHHHHHCC
YMLVVGDQEAENGAVNVRKYGEQKSETVSLDDFVKKAVAEAKK
EEEEECCCCCCCCCEEHHHHCCCCCCEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2115870; 8969504; 9387221; 9384377; 3027671; 1379177 [H]