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The map label for this gene is leuA

Identifier: 154686964

GI number: 154686964

Start: 2656682

End: 2658238

Strand: Reverse

Name: leuA

Synonym: RBAM_025340

Alternate gene names: 154686964

Gene position: 2658238-2656682 (Counterclockwise)

Preceding gene: 154686965

Following gene: 154686963

Centisome position: 67.84

GC content: 48.49

Gene sequence:

>1557_bases
TTGCGCAAAATTAATGTTTTCGACACGACCCTTCGTGACGGTGAACAATCCCCCGGGGTCAACCTCAATTCCCGGGAGAA
ACTGGCGATAGCCAAGCAGCTTGAAAGACTCGGGGTGGACATTATTGAAGCGGGATTTCCCGCTTCATCCCGAGGTGATT
TTTTAGCCGTGCAGGAAATCGCAAGAACGATTAAAAATTGTTCGGTAACAGGGCTTGCCCGATCTGTCAAAGGTGACATC
GACGCAGCCTGGGAAGCGCTGAAAGACGGGGTCAGCCCGCGGATTCACATCTTTATCGCCACTTCTGACATTCATCTGAA
GCATAAGCTGAAAATGACGCGGGAAGAGGTTAAGGAAAAAGCGGTTGAAATGGTGAAGTATGCAAAAGAACGCTTTCCGA
TCGTTCAATGGTCAGCAGAAGACGCATGCCGCACCGAACTTCCGTTTCTGGCTGAAATCGTTGAAGAAGTGATTGATGCC
GGCGCGAGCGTCATCAACCTGCCGGACACTGTAGGTTACCTGACGCCGGATGAATACGGAAACATCTTTAAATATATGAA
GGAACACGTTCCGAATATCAGCCGGGTAAAGCTTTCAGCCCATTGCCACGATGATCTCGGAATGGCGGTTGCCAACTCGC
TTTCGGCAATTGAGAACGGCGCTGATCAAATTGAATGCGCCATTAACGGCATAGGAGAAAGAGCCGGGAATGCCGCGCTA
GAGGAAATCGCCGTTGCGCTTCATGTCAGAAATGACGCGCATCAAGCCGATTCATCTCTTAATCTTCATGAATTGAAGCG
GACGAGTGATCTTGTCAGCAAACTGACGGGCATGGCCGTTCCCCGCAATAAAGCAATCGTCGGAGATAACGCTTTCGCAC
ATGAGTCCGGCATTCACCAAGACGGTTTCCTGAAAGAAAAATCAACATATGAAATCATTTCACCGGAACTTGTCGGCGTG
ACTGCTGATGCGCTCGTACTCGGAAAGCATTCCGGGCGCCACGCGTTCAAAGACCGCCTGAATACGCTTGGATTCCAGTT
TGATAGTGATGAAATCAATAAATATTTTACGATGTTTAAAGAACTGACCGAAAAGAAAAAAGAAATTACCGATGATGACC
TGATTTCTCTTATTTTAGAAGAAAAAGTGACAGACAAAAAAATCGGCTATGAATTTTTGTCTCTGCAAGTGCATTACGGG
ACGTCTCAGGTGCCGACTGCAACAGTCTCTTTAAAAAATCAGGAAAACGAACAGCTGATGCAAGAGGCGGCAACGGGGGC
GGGCAGCGTAGAAGCCGTTTATAATACGCTCGGGCGCTGCATCGATAAAAATATTGAACTGACAGACTACCGCATTCAAT
CCAACCGGAAAGGCGAAGACGCGCTGGCTCAAGTTTTTGTCAGAGTGACCGTGAACGGAAAAGAATCATCAGGGCGGGGA
ATCGCGCAGGATGTTCTGGAAGCTTCTGCTAAAGCTTATCTGAACGCCGTGAACCGGCAGCTGGTGCTGGATTGCAATTT
AGACGGATTAAAGAGACAGACTGCGGTCGGCTCGTAA

Upstream 100 bases:

>100_bases
GCAAGTGAAAACGAGCATCAGATTGAAAAGGTCGGCAGACAGCTTCGTGAAATGATGCCGTTTGTCAAACAAGGCAAAAA
GAAGGAAGCGGTGGTCTCTG

Downstream 100 bases:

>100_bases
TAGAAAGGAGAACGTTTGATTTGAAAAAACGCATTGCTTTGCTGCCCGGTGACGGAATCGGACCGGAAGTGGCGGAATCA
GCGGCAGATGTATTGAAAAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MRKINVFDTTLRDGEQSPGVNLNSREKLAIAKQLERLGVDIIEAGFPASSRGDFLAVQEIARTIKNCSVTGLARSVKGDI
DAAWEALKDGVSPRIHIFIATSDIHLKHKLKMTREEVKEKAVEMVKYAKERFPIVQWSAEDACRTELPFLAEIVEEVIDA
GASVINLPDTVGYLTPDEYGNIFKYMKEHVPNISRVKLSAHCHDDLGMAVANSLSAIENGADQIECAINGIGERAGNAAL
EEIAVALHVRNDAHQADSSLNLHELKRTSDLVSKLTGMAVPRNKAIVGDNAFAHESGIHQDGFLKEKSTYEIISPELVGV
TADALVLGKHSGRHAFKDRLNTLGFQFDSDEINKYFTMFKELTEKKKEITDDDLISLILEEKVTDKKIGYEFLSLQVHYG
TSQVPTATVSLKNQENEQLMQEAATGAGSVEAVYNTLGRCIDKNIELTDYRIQSNRKGEDALAQVFVRVTVNGKESSGRG
IAQDVLEASAKAYLNAVNRQLVLDCNLDGLKRQTAVGS

Sequences:

>Translated_518_residues
MRKINVFDTTLRDGEQSPGVNLNSREKLAIAKQLERLGVDIIEAGFPASSRGDFLAVQEIARTIKNCSVTGLARSVKGDI
DAAWEALKDGVSPRIHIFIATSDIHLKHKLKMTREEVKEKAVEMVKYAKERFPIVQWSAEDACRTELPFLAEIVEEVIDA
GASVINLPDTVGYLTPDEYGNIFKYMKEHVPNISRVKLSAHCHDDLGMAVANSLSAIENGADQIECAINGIGERAGNAAL
EEIAVALHVRNDAHQADSSLNLHELKRTSDLVSKLTGMAVPRNKAIVGDNAFAHESGIHQDGFLKEKSTYEIISPELVGV
TADALVLGKHSGRHAFKDRLNTLGFQFDSDEINKYFTMFKELTEKKKEITDDDLISLILEEKVTDKKIGYEFLSLQVHYG
TSQVPTATVSLKNQENEQLMQEAATGAGSVEAVYNTLGRCIDKNIELTDYRIQSNRKGEDALAQVFVRVTVNGKESSGRG
IAQDVLEASAKAYLNAVNRQLVLDCNLDGLKRQTAVGS
>Mature_518_residues
MRKINVFDTTLRDGEQSPGVNLNSREKLAIAKQLERLGVDIIEAGFPASSRGDFLAVQEIARTIKNCSVTGLARSVKGDI
DAAWEALKDGVSPRIHIFIATSDIHLKHKLKMTREEVKEKAVEMVKYAKERFPIVQWSAEDACRTELPFLAEIVEEVIDA
GASVINLPDTVGYLTPDEYGNIFKYMKEHVPNISRVKLSAHCHDDLGMAVANSLSAIENGADQIECAINGIGERAGNAAL
EEIAVALHVRNDAHQADSSLNLHELKRTSDLVSKLTGMAVPRNKAIVGDNAFAHESGIHQDGFLKEKSTYEIISPELVGV
TADALVLGKHSGRHAFKDRLNTLGFQFDSDEINKYFTMFKELTEKKKEITDDDLISLILEEKVTDKKIGYEFLSLQVHYG
TSQVPTATVSLKNQENEQLMQEAATGAGSVEAVYNTLGRCIDKNIELTDYRIQSNRKGEDALAQVFVRVTVNGKESSGRG
IAQDVLEASAKAYLNAVNRQLVLDCNLDGLKRQTAVGS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=497, Percent_Identity=45.6740442655936, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320071, Length=387, Percent_Identity=28.1653746770026, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=429, Percent_Identity=27.7389277389277, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=561, Percent_Identity=24.777183600713, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=536, Percent_Identity=24.6268656716418, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24582381, Length=280, Percent_Identity=26.4285714285714, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_BACA2 (A7Z7B8)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422125.1
- ProteinModelPortal:   A7Z7B8
- SMR:   A7Z7B8
- STRING:   A7Z7B8
- EnsemblBacteria:   EBBACT00000007774
- GeneID:   5462135
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_025340
- eggNOG:   COG0119
- GeneTree:   EBGT00050000000890
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   BAMY326423:RBAM_025340-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56951; Mature: 56951

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKINVFDTTLRDGEQSPGVNLNSREKLAIAKQLERLGVDIIEAGFPASSRGDFLAVQEI
CCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHH
ARTIKNCSVTGLARSVKGDIDAAWEALKDGVSPRIHIFIATSDIHLKHKLKMTREEVKEK
HHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECCEEHHHHHHHHHHHHHHH
AVEMVKYAKERFPIVQWSAEDACRTELPFLAEIVEEVIDAGASVINLPDTVGYLTPDEYG
HHHHHHHHHHHCCEEEECCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHH
NIFKYMKEHVPNISRVKLSAHCHDDLGMAVANSLSAIENGADQIECAINGIGERAGNAAL
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHH
EEIAVALHVRNDAHQADSSLNLHELKRTSDLVSKLTGMAVPRNKAIVGDNAFAHESGIHQ
HHHHHHHEECCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCC
DGFLKEKSTYEIISPELVGVTADALVLGKHSGRHAFKDRLNTLGFQFDSDEINKYFTMFK
CCCCCCCCCCEECCCHHCCEECCEEEEECCCCCHHHHHHHHHCCCEECHHHHHHHHHHHH
ELTEKKKEITDDDLISLILEEKVTDKKIGYEFLSLQVHYGTSQVPTATVSLKNQENEQLM
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCEEEEEECCCHHHHHH
QEAATGAGSVEAVYNTLGRCIDKNIELTDYRIQSNRKGEDALAQVFVRVTVNGKESSGRG
HHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHEEEEEEEECCCCCCCCC
IAQDVLEASAKAYLNAVNRQLVLDCNLDGLKRQTAVGS
HHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCC
>Mature Secondary Structure
MRKINVFDTTLRDGEQSPGVNLNSREKLAIAKQLERLGVDIIEAGFPASSRGDFLAVQEI
CCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHH
ARTIKNCSVTGLARSVKGDIDAAWEALKDGVSPRIHIFIATSDIHLKHKLKMTREEVKEK
HHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECCEEHHHHHHHHHHHHHHH
AVEMVKYAKERFPIVQWSAEDACRTELPFLAEIVEEVIDAGASVINLPDTVGYLTPDEYG
HHHHHHHHHHHCCEEEECCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHH
NIFKYMKEHVPNISRVKLSAHCHDDLGMAVANSLSAIENGADQIECAINGIGERAGNAAL
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHH
EEIAVALHVRNDAHQADSSLNLHELKRTSDLVSKLTGMAVPRNKAIVGDNAFAHESGIHQ
HHHHHHHEECCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCC
DGFLKEKSTYEIISPELVGVTADALVLGKHSGRHAFKDRLNTLGFQFDSDEINKYFTMFK
CCCCCCCCCCEECCCHHCCEECCEEEEECCCCCHHHHHHHHHCCCEECHHHHHHHHHHHH
ELTEKKKEITDDDLISLILEEKVTDKKIGYEFLSLQVHYGTSQVPTATVSLKNQENEQLM
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCEEEEEECCCHHHHHH
QEAATGAGSVEAVYNTLGRCIDKNIELTDYRIQSNRKGEDALAQVFVRVTVNGKESSGRG
HHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHEEEEEEEECCCCCCCCC
IAQDVLEASAKAYLNAVNRQLVLDCNLDGLKRQTAVGS
HHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA