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The map label for this gene is leuB [H]

Identifier: 154686963

GI number: 154686963

Start: 2655564

End: 2656661

Strand: Reverse

Name: leuB [H]

Synonym: RBAM_025330

Alternate gene names: 154686963

Gene position: 2656661-2655564 (Counterclockwise)

Preceding gene: 154686964

Following gene: 154686962

Centisome position: 67.8

GC content: 51.37

Gene sequence:

>1098_bases
TTGAAAAAACGCATTGCTTTGCTGCCCGGTGACGGAATCGGACCGGAAGTGGCGGAATCAGCGGCAGATGTATTGAAAAG
CGTTGCCGAACAATTCGGACATGAATTTGAATTTGAAAACGGACTGATCGGGGGAGCGGCCATTGACGCCCATCAAAACC
CCCTTCCTGAGGAGACGGTGGCTCTTTGTGAAAGATCCGACGCCATCCTGCTCGGGGCAGTCGGCGGACCGAAATGGGAC
CAAAATCCTCCGGAGCTCAGACCGGAAAAAGGCCTGCTCGGCATCAGAAAAAAACTCGGCCTGTTTGCCAATCTGCGTCC
CGTGACCGTATTTGAAAGCCTGTCTGAGGCGTCTCCGCTGAAAAAAGAATATATCAGCGGCGTCGACTTTGTAATCGTCA
GGGAACTGACGGGCGGCCTCTATTTCGGAGAACCGAGCAGACAGTATGTCAATGAAGCGGGAGAAAAGGAAGCGGTCGAT
ACCCTTTTTTATAAGAAAGAAGAAATCGAACGCGTTGTCAGAGAAGCCTTTAAGATGGCTGCCGGCAGAAAAGGAAAAGT
GACGTCCGTTGATAAGGCGAATGTGCTTTCTTCAAGCAGGCTGTGGCGGGAAACCGCAGAGGAAATCGCAAAAGAATTTC
CTGACGTGACGCTTGAACATATGCTTGTCGATAATGCCGCGATGCAGCTCATCTACGCTCCGAGCCAATTTGACGTCATC
GTAACTGAGAATATGTTCGGCGATATTTTAAGCGATGAAGCCTCCATGCTGACAGGCTCGCTCGGCATGCTGCCGTCAGC
CAGTTTATCAAGTTCAGGTCTTCACTTGTTTGAACCCGTGCATGGATCTGCGCCTGACATCGCCGGTAAAGGGATGGCCA
ATCCGTTTGCGGCGATTTTATCAGCGGCGATGCTTTTGAGAACATCTTTCGGGCTTGAAAACGAAGCGCAGGCGGTGGAA
AAGGCGGTCGCAAAGGTGCTTGCAGACGGCATCCGTACACGTGATATCGCCCGGGACGGCCAATATGCCGGAACGCATGA
CATTACTGAAAAAGTAAAAGCCGCATTAGCCGGCTCGAATACAAAATCTATAGTGTGA

Upstream 100 bases:

>100_bases
ATCTGAACGCCGTGAACCGGCAGCTGGTGCTGGATTGCAATTTAGACGGATTAAAGAGACAGACTGCGGTCGGCTCGTAA
TAGAAAGGAGAACGTTTGAT

Downstream 100 bases:

>100_bases
CGCTTTAAAGAAAGCGGTCTTAGTTCATAGGTAGAGGGAGGAAACAAAGATGATGCCTCGAACAATCATCGAAAAGATTT
GGGATCGGCATGTTGTAAAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKKRIALLPGDGIGPEVAESAADVLKSVAEQFGHEFEFENGLIGGAAIDAHQNPLPEETVALCERSDAILLGAVGGPKWD
QNPPELRPEKGLLGIRKKLGLFANLRPVTVFESLSEASPLKKEYISGVDFVIVRELTGGLYFGEPSRQYVNEAGEKEAVD
TLFYKKEEIERVVREAFKMAAGRKGKVTSVDKANVLSSSRLWRETAEEIAKEFPDVTLEHMLVDNAAMQLIYAPSQFDVI
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAILSAAMLLRTSFGLENEAQAVE
KAVAKVLADGIRTRDIARDGQYAGTHDITEKVKAALAGSNTKSIV

Sequences:

>Translated_365_residues
MKKRIALLPGDGIGPEVAESAADVLKSVAEQFGHEFEFENGLIGGAAIDAHQNPLPEETVALCERSDAILLGAVGGPKWD
QNPPELRPEKGLLGIRKKLGLFANLRPVTVFESLSEASPLKKEYISGVDFVIVRELTGGLYFGEPSRQYVNEAGEKEAVD
TLFYKKEEIERVVREAFKMAAGRKGKVTSVDKANVLSSSRLWRETAEEIAKEFPDVTLEHMLVDNAAMQLIYAPSQFDVI
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAILSAAMLLRTSFGLENEAQAVE
KAVAKVLADGIRTRDIARDGQYAGTHDITEKVKAALAGSNTKSIV
>Mature_365_residues
MKKRIALLPGDGIGPEVAESAADVLKSVAEQFGHEFEFENGLIGGAAIDAHQNPLPEETVALCERSDAILLGAVGGPKWD
QNPPELRPEKGLLGIRKKLGLFANLRPVTVFESLSEASPLKKEYISGVDFVIVRELTGGLYFGEPSRQYVNEAGEKEAVD
TLFYKKEEIERVVREAFKMAAGRKGKVTSVDKANVLSSSRLWRETAEEIAKEFPDVTLEHMLVDNAAMQLIYAPSQFDVI
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAILSAAMLLRTSFGLENEAQAVE
KAVAKVLADGIRTRDIARDGQYAGTHDITEKVKAALAGSNTKSIV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=358, Percent_Identity=32.122905027933, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI28178816, Length=359, Percent_Identity=31.1977715877437, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI28178821, Length=355, Percent_Identity=30.1408450704225, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI4758582, Length=344, Percent_Identity=30.5232558139535, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI28178838, Length=326, Percent_Identity=30.6748466257669, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178819, Length=214, Percent_Identity=32.2429906542056, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=52.247191011236, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1788101, Length=344, Percent_Identity=37.5, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1787381, Length=286, Percent_Identity=26.2237762237762, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=358, Percent_Identity=33.2402234636872, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17550882, Length=358, Percent_Identity=32.9608938547486, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=361, Percent_Identity=32.409972299169, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=346, Percent_Identity=30.3468208092486, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=367, Percent_Identity=48.5013623978202, Blast_Score=312, Evalue=6e-86,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=35.8695652173913, Blast_Score=169, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=368, Percent_Identity=33.1521739130435, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=364, Percent_Identity=32.4175824175824, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643270, Length=339, Percent_Identity=32.7433628318584, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24643268, Length=339, Percent_Identity=32.7433628318584, Blast_Score=151, Evalue=6e-37,
Organism=Drosophila melanogaster, GI281362242, Length=357, Percent_Identity=31.3725490196078, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24648872, Length=357, Percent_Identity=31.3725490196078, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24661184, Length=347, Percent_Identity=30.5475504322767, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI161078633, Length=366, Percent_Identity=27.8688524590164, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24650122, Length=366, Percent_Identity=27.8688524590164, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078637, Length=366, Percent_Identity=27.8688524590164, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078635, Length=366, Percent_Identity=27.8688524590164, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161078639, Length=361, Percent_Identity=27.9778393351801, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI20130355, Length=337, Percent_Identity=26.1127596439169, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39228; Mature: 39228

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIALLPGDGIGPEVAESAADVLKSVAEQFGHEFEFENGLIGGAAIDAHQNPLPEETV
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCEEECCCCCCCCHHHH
ALCERSDAILLGAVGGPKWDQNPPELRPEKGLLGIRKKLGLFANLRPVTVFESLSEASPL
HHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHH
KKEYISGVDFVIVRELTGGLYFGEPSRQYVNEAGEKEAVDTLFYKKEEIERVVREAFKMA
HHHHHCCCHHEEHHHHHCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRKGKVTSVDKANVLSSSRLWRETAEEIAKEFPDVTLEHMLVDNAAMQLIYAPSQFDVI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCCEEE
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAIL
EECHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHH
SAAMLLRTSFGLENEAQAVEKAVAKVLADGIRTRDIARDGQYAGTHDITEKVKAALAGSN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
TKSIV
CCCCC
>Mature Secondary Structure
MKKRIALLPGDGIGPEVAESAADVLKSVAEQFGHEFEFENGLIGGAAIDAHQNPLPEETV
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCEEECCCCCCCCHHHH
ALCERSDAILLGAVGGPKWDQNPPELRPEKGLLGIRKKLGLFANLRPVTVFESLSEASPL
HHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHH
KKEYISGVDFVIVRELTGGLYFGEPSRQYVNEAGEKEAVDTLFYKKEEIERVVREAFKMA
HHHHHCCCHHEEHHHHHCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRKGKVTSVDKANVLSSSRLWRETAEEIAKEFPDVTLEHMLVDNAAMQLIYAPSQFDVI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCCEEE
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAIL
EECHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHH
SAAMLLRTSFGLENEAQAVEKAVAKVLADGIRTRDIARDGQYAGTHDITEKVKAALAGSN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
TKSIV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3110742; 8969504; 9384377 [H]