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The map label for this gene is greA

Identifier: 154686872

GI number: 154686872

Start: 2562096

End: 2562569

Strand: Reverse

Name: greA

Synonym: RBAM_024420

Alternate gene names: 154686872

Gene position: 2562569-2562096 (Counterclockwise)

Preceding gene: 154686873

Following gene: 154686871

Centisome position: 65.4

GC content: 43.67

Gene sequence:

>474_bases
ATGGCACAAGAAAAAGTTTTTCCGATGACTCACGAAGGAAAACAAAAACTAGAACAAGAATTAGAGCAATTGAAAACGGT
TAAACGTAAAGAAGTAGTAGAACGCATTAAAATCGCAAGAAGCTTCGGAGATCTGTCAGAGAACTCTGAGTATGATTCTG
CAAAAGAAGAACAGGCATTTGTGGAAGGGCGCATCACGACGCTTGAAAACATGATCCGCAATGCGAAAATCATCGAGGAC
GACGGCGGTTCAAATGTAGTCGGTTTGGGAAAAACCGTATCATTCATCGAATTGCCGGACGGTGAAGAAGAATCTTACAC
GATTGTCGGAAGCGCTGAAGCTGATCCGTTTGAAGGGAAAATTTCAAACGACTCGCCAATCGCCAAAAGCCTGCTCGGCA
GAAAAGTGGACGAAGAAGTGACAGTTCAGACGCCGGGCGGAGAAATGCTCGTGAAAATTGTGAAAATTTCATAA

Upstream 100 bases:

>100_bases
CGAGGACTGCCCTGTGTTCTCATTCTTGATTTTTTCGTTTATGCTCTCATACATAATCACACGTTATTGTTGAGGGACAA
CCACCAAGGAGTGAAGGAAC

Downstream 100 bases:

>100_bases
GATCCGTTTAACAATGAAACACCTCGTCCAAAATGTGGATGAGGTGTTTTTTTATGATCGTATCAAAAAGGCTGAAAGCG
GCCGTGATTTGTTTTCTGCT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAQEKVFPMTHEGKQKLEQELEQLKTVKRKEVVERIKIARSFGDLSENSEYDSAKEEQAFVEGRITTLENMIRNAKIIED
DGGSNVVGLGKTVSFIELPDGEEESYTIVGSAEADPFEGKISNDSPIAKSLLGRKVDEEVTVQTPGGEMLVKIVKIS

Sequences:

>Translated_157_residues
MAQEKVFPMTHEGKQKLEQELEQLKTVKRKEVVERIKIARSFGDLSENSEYDSAKEEQAFVEGRITTLENMIRNAKIIED
DGGSNVVGLGKTVSFIELPDGEEESYTIVGSAEADPFEGKISNDSPIAKSLLGRKVDEEVTVQTPGGEMLVKIVKIS
>Mature_156_residues
AQEKVFPMTHEGKQKLEQELEQLKTVKRKEVVERIKIARSFGDLSENSEYDSAKEEQAFVEGRITTLENMIRNAKIIEDD
GGSNVVGLGKTVSFIELPDGEEESYTIVGSAEADPFEGKISNDSPIAKSLLGRKVDEEVTVQTPGGEMLVKIVKIS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=42.8571428571429, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI87082261, Length=141, Percent_Identity=36.1702127659575, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_BACA2 (A7Z726)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422033.1
- ProteinModelPortal:   A7Z726
- SMR:   A7Z726
- STRING:   A7Z726
- EnsemblBacteria:   EBBACT00000004665
- GeneID:   5462357
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_024420
- eggNOG:   COG0782
- GeneTree:   EBGT00050000001871
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   BAMY326423:RBAM_024420-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17359; Mature: 17228

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQEKVFPMTHEGKQKLEQELEQLKTVKRKEVVERIKIARSFGDLSENSEYDSAKEEQAF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VEGRITTLENMIRNAKIIEDDGGSNVVGLGKTVSFIELPDGEEESYTIVGSAEADPFEGK
HHHHHHHHHHHHHCCEEEECCCCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCCCCCC
ISNDSPIAKSLLGRKVDEEVTVQTPGGEMLVKIVKIS
CCCCCHHHHHHHCCCCCCCEEEECCCHHEEEEEEECC
>Mature Secondary Structure 
AQEKVFPMTHEGKQKLEQELEQLKTVKRKEVVERIKIARSFGDLSENSEYDSAKEEQAF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VEGRITTLENMIRNAKIIEDDGGSNVVGLGKTVSFIELPDGEEESYTIVGSAEADPFEGK
HHHHHHHHHHHHHCCEEEECCCCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCCCCCC
ISNDSPIAKSLLGRKVDEEVTVQTPGGEMLVKIVKIS
CCCCCHHHHHHHCCCCCCCEEEECCCHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA