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The map label for this gene is nodB [H]

Identifier: 154686831

GI number: 154686831

Start: 2518229

End: 2518963

Strand: Reverse

Name: nodB [H]

Synonym: RBAM_024010

Alternate gene names: 154686831

Gene position: 2518963-2518229 (Counterclockwise)

Preceding gene: 154686833

Following gene: 154686830

Centisome position: 64.28

GC content: 46.94

Gene sequence:

>735_bases
TTGAGATACTTTCATCTGATCATCATGATATGCGTCCTCACGAGTCTCACTGCCGTGTCCGCCGATGCGAAATCTTTAAA
AAGAAGTGACATGGAGAAGACGGGAAACGTGTACTGGGATATAAGGTCTGAAAAAAAGAAACTATCTTTAACCTTCGATG
ACGGCCCCCATCCTGTCTATACGGAGGCTGTGTTAGACGTGTTGAAACAGCACAATGTTAAAGCAACCTTTTTTTGTGTC
GGGAGCCGTATAGACAAATACCCCATAACGGCAAAACGCATCGTTGAAGAAGGGCATGAAATCGGCAACCATACGATGAA
CCATGTTTATTTTCACCGACTGAAACGCAAAGACGTTTTACAAGAGCTTCACAGCTCAGCGCATCATATCACATCGCTGC
AGCCGGAAGGGGACAAGTTATTCCGCCCGCCGGGAGGGTCGCTGAATCATACGGCTTTCAAAAGCATCTTAAAAGAGGGC
TATCATATCATCATGTGGTCATGGAACCAAGATCCGAGGGACTGGAAAAGGCCCGGGAAAGGAAAAATCGTCAGGCACGT
TGTTTCACATGCCAGAGATGGAGATATCGTTCTTCTGCATGACGGCGGAGGAAACCGGCGCCAGACGGTTGAGGCTTTGA
AAGAAATCATTCCCAAGCTGAAAAAGCAGGGCTACACGTTTGTTAAGGTTTCGGATTTGATCGGACAGGATTCCGGCCCT
TTTCATATCCAGTAA

Upstream 100 bases:

>100_bases
AAAACAAGCGGGAAGCGGGTGATGTGCATCATTTGATGCAGAGAATCATTTTTTATAAAAGGGGACACGTTAACAGTAGT
GTTTACTGGAGGTGTAAACG

Downstream 100 bases:

>100_bases
AGAGAGCGCCGCGTTGAAAGCCGCCAAAGAGATGTGTACCATAGGAGGTAAGGTTTGGGGGGAGAGAAAATGAAGTTTGC
TGTTCTTTTTATTACGATAA

Product: putative polysaccharide deacetylase

Products: NA

Alternate protein names: Nodulation protein B [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRYFHLIIMICVLTSLTAVSADAKSLKRSDMEKTGNVYWDIRSEKKKLSLTFDDGPHPVYTEAVLDVLKQHNVKATFFCV
GSRIDKYPITAKRIVEEGHEIGNHTMNHVYFHRLKRKDVLQELHSSAHHITSLQPEGDKLFRPPGGSLNHTAFKSILKEG
YHIIMWSWNQDPRDWKRPGKGKIVRHVVSHARDGDIVLLHDGGGNRRQTVEALKEIIPKLKKQGYTFVKVSDLIGQDSGP
FHIQ

Sequences:

>Translated_244_residues
MRYFHLIIMICVLTSLTAVSADAKSLKRSDMEKTGNVYWDIRSEKKKLSLTFDDGPHPVYTEAVLDVLKQHNVKATFFCV
GSRIDKYPITAKRIVEEGHEIGNHTMNHVYFHRLKRKDVLQELHSSAHHITSLQPEGDKLFRPPGGSLNHTAFKSILKEG
YHIIMWSWNQDPRDWKRPGKGKIVRHVVSHARDGDIVLLHDGGGNRRQTVEALKEIIPKLKKQGYTFVKVSDLIGQDSGP
FHIQ
>Mature_244_residues
MRYFHLIIMICVLTSLTAVSADAKSLKRSDMEKTGNVYWDIRSEKKKLSLTFDDGPHPVYTEAVLDVLKQHNVKATFFCV
GSRIDKYPITAKRIVEEGHEIGNHTMNHVYFHRLKRKDVLQELHSSAHHITSLQPEGDKLFRPPGGSLNHTAFKSILKEG
YHIIMWSWNQDPRDWKRPGKGKIVRHVVSHARDGDIVLLHDGGGNRRQTVEALKEIIPKLKKQGYTFVKVSDLIGQDSGP
FHIQ

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323338, Length=198, Percent_Identity=29.7979797979798, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323339, Length=164, Percent_Identity=31.0975609756098, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 27906; Mature: 27906

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYFHLIIMICVLTSLTAVSADAKSLKRSDMEKTGNVYWDIRSEKKKLSLTFDDGPHPVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCHH
TEAVLDVLKQHNVKATFFCVGSRIDKYPITAKRIVEEGHEIGNHTMNHVYFHRLKRKDVL
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QELHSSAHHITSLQPEGDKLFRPPGGSLNHTAFKSILKEGYHIIMWSWNQDPRDWKRPGK
HHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHCCCCC
GKIVRHVVSHARDGDIVLLHDGGGNRRQTVEALKEIIPKLKKQGYTFVKVSDLIGQDSGP
CHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCC
FHIQ
CCCC
>Mature Secondary Structure
MRYFHLIIMICVLTSLTAVSADAKSLKRSDMEKTGNVYWDIRSEKKKLSLTFDDGPHPVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCHH
TEAVLDVLKQHNVKATFFCVGSRIDKYPITAKRIVEEGHEIGNHTMNHVYFHRLKRKDVL
HHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QELHSSAHHITSLQPEGDKLFRPPGGSLNHTAFKSILKEGYHIIMWSWNQDPRDWKRPGK
HHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHCCCCC
GKIVRHVVSHARDGDIVLLHDGGGNRRQTVEALKEIIPKLKKQGYTFVKVSDLIGQDSGP
CHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCC
FHIQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3960737 [H]