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The map label for this gene is folD

Identifier: 154686695

GI number: 154686695

Start: 2402199

End: 2403050

Strand: Reverse

Name: folD

Synonym: RBAM_022640

Alternate gene names: 154686695

Gene position: 2403050-2402199 (Counterclockwise)

Preceding gene: 154686696

Following gene: 154686694

Centisome position: 61.32

GC content: 46.83

Gene sequence:

>852_bases
ATGACTGCAACAATCATCGACGGAAAAGAAACGGCTAAAGAAAAACGCGAACAATTGGCAAAAGAAGTAGAAGAGCTGAA
AGCACAAGGCGTAGTGCCTGGCTTGGCGGTCATTTTAATCGGAGACGATCCCGCTTCTGTTTCATATGTGACAGGCAAGA
AAAAAGCCGCTGAAACAATGGGAATGAAATTTAAGCTTGACCGCTTTGACTCAAGCTTAACCGAGGCGGAGCTTTTAACA
GTGATTGATCAGTATAATCAAAATCCGGAATTCCACGGCATTCTCGTCCAGCTGCCGCTCCCGGATCATATTTCTGAAAA
AGCCGTAATCGAGCGGATCTCCCCTGATAAGGATGTTGACGGTTTCCACCCTCTCAACGTCGGGAAAATGCTGCTTGGCG
AAGATACATTTCTTCCATGCACGCCGCACGGAATTGTCGAGTTATTGAAAAAGACGAACGTAGATCTTTCCGGCAAAGAA
GTTGTTGTAGTCGGACGAAGCAATATTGTCGGTAAACCGGTCGGCCAGCTGCTGCTGAACGAAAACGCGACCGTCACATA
TTGCCATTCCCGCACGAAAAATATGTCTGAGCATACGAAAAAAGCAGATATTCTTGTGGTAGCGGTCGGTAAAGCGAACT
TCATCAAAGCTGATCAAATCAAAGAGGGCGCCATTGTCATTGATGTCGGCGTCAACCGTCTCGAAAGCGGAAAACTCTGC
GGAGACGTTCAGTTTGATGAAGCGAAAGAAAAAGCATCTTTCATTACGCCGGTGCCGGGCGGAGTAGGTCCGATGACCAT
CACAATGCTTGCGCATAATACTGTTAAATCTGCCAGACGTACGTTATCATAG

Upstream 100 bases:

>100_bases
TCGAGCTGGCAAAACGGTTCGGCGACGATAAAGCTGCAAAATTCGTAAACGGGGTTCTTTCTAACATTAAAACTGATATC
GAATCATAGGAGGAAAGAAA

Downstream 100 bases:

>100_bases
ATAACCGGAGACAGTCACAAAAATTGTGACAATGAGCACGATACCCGCTTCTTAAGGCTCCTTTCAGCCAGAGCGGGTTT
TCTTAGTTTAGGGCAAAAAG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTATIIDGKETAKEKREQLAKEVEELKAQGVVPGLAVILIGDDPASVSYVTGKKKAAETMGMKFKLDRFDSSLTEAELLT
VIDQYNQNPEFHGILVQLPLPDHISEKAVIERISPDKDVDGFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNVDLSGKE
VVVVGRSNIVGKPVGQLLLNENATVTYCHSRTKNMSEHTKKADILVVAVGKANFIKADQIKEGAIVIDVGVNRLESGKLC
GDVQFDEAKEKASFITPVPGGVGPMTITMLAHNTVKSARRTLS

Sequences:

>Translated_283_residues
MTATIIDGKETAKEKREQLAKEVEELKAQGVVPGLAVILIGDDPASVSYVTGKKKAAETMGMKFKLDRFDSSLTEAELLT
VIDQYNQNPEFHGILVQLPLPDHISEKAVIERISPDKDVDGFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNVDLSGKE
VVVVGRSNIVGKPVGQLLLNENATVTYCHSRTKNMSEHTKKADILVVAVGKANFIKADQIKEGAIVIDVGVNRLESGKLC
GDVQFDEAKEKASFITPVPGGVGPMTITMLAHNTVKSARRTLS
>Mature_282_residues
TATIIDGKETAKEKREQLAKEVEELKAQGVVPGLAVILIGDDPASVSYVTGKKKAAETMGMKFKLDRFDSSLTEAELLTV
IDQYNQNPEFHGILVQLPLPDHISEKAVIERISPDKDVDGFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNVDLSGKEV
VVVGRSNIVGKPVGQLLLNENATVTYCHSRTKNMSEHTKKADILVVAVGKANFIKADQIKEGAIVIDVGVNRLESGKLCG
DVQFDEAKEKASFITPVPGGVGPMTITMLAHNTVKSARRTLS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=47.6027397260274, Blast_Score=256, Evalue=1e-68,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=45.6081081081081, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=43.5810810810811, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI36796743, Length=273, Percent_Identity=26.7399267399267, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=51.9713261648746, Blast_Score=285, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=41.7808219178082, Blast_Score=211, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=44.3333333333333, Blast_Score=256, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=44.7916666666667, Blast_Score=248, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6322933, Length=311, Percent_Identity=27.6527331189711, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=44.5945945945946, Blast_Score=245, Evalue=3e-65,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=44.5945945945946, Blast_Score=244, Evalue=3e-65,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=45.360824742268, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=45.360824742268, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=45.360824742268, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=45.360824742268, Blast_Score=244, Evalue=7e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_BACA2 (A7Z6J9)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421856.1
- ProteinModelPortal:   A7Z6J9
- SMR:   A7Z6J9
- STRING:   A7Z6J9
- EnsemblBacteria:   EBBACT00000006955
- GeneID:   5459560
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_022640
- eggNOG:   COG0190
- GeneTree:   EBGT00050000002737
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- ProtClustDB:   PRK14190
- BioCyc:   BAMY326423:RBAM_022640-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30621; Mature: 30490

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATIIDGKETAKEKREQLAKEVEELKAQGVVPGLAVILIGDDPASVSYVTGKKKAAETM
CEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCHHHHHHH
GMKFKLDRFDSSLTEAELLTVIDQYNQNPEFHGILVQLPLPDHISEKAVIERISPDKDVD
CCEEEEHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCC
GFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNVDLSGKEVVVVGRSNIVGKPVGQLLLN
CCCCCCCCHHEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHCC
ENATVTYCHSRTKNMSEHTKKADILVVAVGKANFIKADQIKEGAIVIDVGVNRLESGKLC
CCCEEEEECCCCCCHHHHCCCCCEEEEEECCCCCEECCCCCCCCEEEEECCCCCCCCCEE
GDVQFDEAKEKASFITPVPGGVGPMTITMLAHNTVKSARRTLS
CCCCHHHHHHHCCEECCCCCCCCCEEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
TATIIDGKETAKEKREQLAKEVEELKAQGVVPGLAVILIGDDPASVSYVTGKKKAAETM
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCHHHHHHH
GMKFKLDRFDSSLTEAELLTVIDQYNQNPEFHGILVQLPLPDHISEKAVIERISPDKDVD
CCEEEEHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCC
GFHPLNVGKMLLGEDTFLPCTPHGIVELLKKTNVDLSGKEVVVVGRSNIVGKPVGQLLLN
CCCCCCCCHHEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHCC
ENATVTYCHSRTKNMSEHTKKADILVVAVGKANFIKADQIKEGAIVIDVGVNRLESGKLC
CCCEEEEECCCCCCHHHHCCCCCEEEEEECCCCCEECCCCCCCCEEEEECCCCCCCCCEE
GDVQFDEAKEKASFITPVPGGVGPMTITMLAHNTVKSARRTLS
CCCCHHHHHHHCCEECCCCCCCCCEEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA