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The map label for this gene is rnz

Identifier: 154686646

GI number: 154686646

Start: 2351534

End: 2352460

Strand: Reverse

Name: rnz

Synonym: RBAM_022150

Alternate gene names: 154686646

Gene position: 2352460-2351534 (Counterclockwise)

Preceding gene: 154686648

Following gene: 154686645

Centisome position: 60.03

GC content: 49.3

Gene sequence:

>927_bases
GTGGAATTATTATTTTTAGGGACGGGGGCCGGCATGCCGGCAAAGACGAGAAACGTAACGTCCGTCGCGCTGAAGCTTCT
TGAAGAAAGGCGTTCCGTATGGCTGTTTGATTGCGGAGAAGCCACTCAGCATCAGATATTACATACAACAATCAAACCGA
GGAAAATTGAGAAGATTTTCATCACCCACCTTCACGGCGACCATGTTTACGGTCTTCCGGGTCTGTTGGGGAGCCGTTCA
TTTCAGGGCGGTGAAGAAGAGCTGACGATTTACGGGCCAAAAGGCATTAAAGCCTTTATCGAAACAAGCCTGAACGTGAC
GGCGACTCATCTGACGTATCCTCTTACCGTACATGAAATCGAAGAGGGGACGGTTTTTGAAGATGAGCAATTCATTGTTA
CCGCAGCATCCGTCATTCACGGCGTGGAAGCGTTCGGCTACCGGGTTCAGGAAAAGGATATTCCCGGCGCATTGCAGGCC
GGCAGATTAAAAGAAATGAATATCCCGCCCGGACCCGTCTATCAGAAAATCAAAAAAGGCGAAACGGTCACACTTGACGA
TGGCAGAATCATTAACGGGCGGGATTTTTTGGAACCCCCGAAAAAAGGGAGAATCGTCGCTTTTTCCGGGGATACGAGGG
CATCAGAGCGGGTAACGGAGCTGGCGCGCAATGCGGATGTGCTTGTTCATGAAGCAACATTTGCAAAGGAAGATGCAAAG
CTCGCACATAATTATTATCACGCGACGACAGAACAGGCTGCGCAAACGGCAAAAAAAGCAGGCGCCAAACAGCTGATCTT
AACTCATATCAGCGCAAGGTATCAGGGTGAATCGCCGATAGAGCGTCTCGAACATGAGGCGAAAGCGGTATTTGAAAACA
GTAAGATCGCATTTGACTTCATGGAGGTCACTGTGGAGAGGTCTTAA

Upstream 100 bases:

>100_bases
TGCGGAAAAAAATTAATCAGGCCGGAGAAGGCCCGGGTGAATTCACATTTTCAGCCTGATCCTGTATCCTTAGTAGTACA
AAGATCTAGGAGGAAGCATT

Downstream 100 bases:

>100_bases
AAAATGGACGCTTGACATTTTTTTCTGGTGAGATATAATAAAACGTAATAATTACGATTTGTGCAAGGGGTGAAAATGAT
GCGTGTGAATGTTACATTGG

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MELLFLGTGAGMPAKTRNVTSVALKLLEERRSVWLFDCGEATQHQILHTTIKPRKIEKIFITHLHGDHVYGLPGLLGSRS
FQGGEEELTIYGPKGIKAFIETSLNVTATHLTYPLTVHEIEEGTVFEDEQFIVTAASVIHGVEAFGYRVQEKDIPGALQA
GRLKEMNIPPGPVYQKIKKGETVTLDDGRIINGRDFLEPPKKGRIVAFSGDTRASERVTELARNADVLVHEATFAKEDAK
LAHNYYHATTEQAAQTAKKAGAKQLILTHISARYQGESPIERLEHEAKAVFENSKIAFDFMEVTVERS

Sequences:

>Translated_308_residues
MELLFLGTGAGMPAKTRNVTSVALKLLEERRSVWLFDCGEATQHQILHTTIKPRKIEKIFITHLHGDHVYGLPGLLGSRS
FQGGEEELTIYGPKGIKAFIETSLNVTATHLTYPLTVHEIEEGTVFEDEQFIVTAASVIHGVEAFGYRVQEKDIPGALQA
GRLKEMNIPPGPVYQKIKKGETVTLDDGRIINGRDFLEPPKKGRIVAFSGDTRASERVTELARNADVLVHEATFAKEDAK
LAHNYYHATTEQAAQTAKKAGAKQLILTHISARYQGESPIERLEHEAKAVFENSKIAFDFMEVTVERS
>Mature_308_residues
MELLFLGTGAGMPAKTRNVTSVALKLLEERRSVWLFDCGEATQHQILHTTIKPRKIEKIFITHLHGDHVYGLPGLLGSRS
FQGGEEELTIYGPKGIKAFIETSLNVTATHLTYPLTVHEIEEGTVFEDEQFIVTAASVIHGVEAFGYRVQEKDIPGALQA
GRLKEMNIPPGPVYQKIKKGETVTLDDGRIINGRDFLEPPKKGRIVAFSGDTRASERVTELARNADVLVHEATFAKEDAK
LAHNYYHATTEQAAQTAKKAGAKQLILTHISARYQGESPIERLEHEAKAVFENSKIAFDFMEVTVERS

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Homo sapiens, GI8922122, Length=360, Percent_Identity=36.1111111111111, Blast_Score=221, Evalue=5e-58,
Organism=Escherichia coli, GI87082078, Length=306, Percent_Identity=47.7124183006536, Blast_Score=302, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI71985814, Length=311, Percent_Identity=28.9389067524116, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71985808, Length=311, Percent_Identity=28.9389067524116, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24652403, Length=204, Percent_Identity=27.9411764705882, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_BACA2 (A7Z6F0)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421807.1
- ProteinModelPortal:   A7Z6F0
- SMR:   A7Z6F0
- STRING:   A7Z6F0
- EnsemblBacteria:   EBBACT00000004783
- GeneID:   5463015
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_022150
- eggNOG:   COG1234
- GeneTree:   EBGT00050000001790
- HOGENOM:   HBG648406
- OMA:   HSTSTMA
- ProtClustDB:   PRK00055
- BioCyc:   BAMY326423:RBAM_022150-MONOMER
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849
- TIGRFAMs:   TIGR02651

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 34175; Mature: 34175

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLFLGTGAGMPAKTRNVTSVALKLLEERRSVWLFDCGEATQHQILHTTIKPRKIEKIF
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHEEECCCCCEEEEEE
ITHLHGDHVYGLPGLLGSRSFQGGEEELTIYGPKGIKAFIETSLNVTATHLTYPLTVHEI
EEEECCCEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCEEEEEEEEEEEEEEC
EEGTVFEDEQFIVTAASVIHGVEAFGYRVQEKDIPGALQAGRLKEMNIPPGPVYQKIKKG
CCCCEECCCCEEEEHHHHHHHHHHHCEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHCC
ETVTLDDGRIINGRDFLEPPKKGRIVAFSGDTRASERVTELARNADVLVHEATFAKEDAK
CEEEECCCEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHH
LAHNYYHATTEQAAQTAKKAGAKQLILTHISARYQGESPIERLEHEAKAVFENSKIAFDF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEE
MEVTVERS
EEEEEECC
>Mature Secondary Structure
MELLFLGTGAGMPAKTRNVTSVALKLLEERRSVWLFDCGEATQHQILHTTIKPRKIEKIF
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHEEECCCCCEEEEEE
ITHLHGDHVYGLPGLLGSRSFQGGEEELTIYGPKGIKAFIETSLNVTATHLTYPLTVHEI
EEEECCCEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCEEEEEEEEEEEEEEC
EEGTVFEDEQFIVTAASVIHGVEAFGYRVQEKDIPGALQAGRLKEMNIPPGPVYQKIKKG
CCCCEECCCCEEEEHHHHHHHHHHHCEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHCC
ETVTLDDGRIINGRDFLEPPKKGRIVAFSGDTRASERVTELARNADVLVHEATFAKEDAK
CEEEECCCEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHH
LAHNYYHATTEQAAQTAKKAGAKQLILTHISARYQGESPIERLEHEAKAVFENSKIAFDF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEE
MEVTVERS
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA