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The map label for this gene is dfnL [H]

Identifier: 154686626

GI number: 154686626

Start: 2277549

End: 2278796

Strand: Reverse

Name: dfnL [H]

Synonym: RBAM_021950

Alternate gene names: 154686626

Gene position: 2278796-2277549 (Counterclockwise)

Preceding gene: 154686627

Following gene: 154686625

Centisome position: 58.15

GC content: 49.12

Gene sequence:

>1248_bases
ATGAATGTAGGAATCGAAGCAATGAACGTGTTTGGCGGAACCGCGTATGTAGACGTCATGGAGCTTGCGGAACATCGGAA
CTTGGATAAAAAAAGGTTTGAGAACCTGCTGATGAAAGAAAAAGTAGTGGCGCTTCCGTTTGAAGATCCGATTACGTTCG
CCGTAAATGCCGCAAAACCAATTATCGACTCTCTGTCAGAAGAGGAACGGAATCAGATTGAAATGGTCATCACCTGTACG
GAATCCGGGATTGATTTTGGCAAATCCATCAGTACATACGTCCATGATTATTTGCAGCTGAAGCGGAATTGCCGGTTATT
TGAGGTGAAACAGGCTTGCTATTCTTTAACGGCCGGGTTTCAAATGGGCGTGAATTTCATCTTATCGCAAACATCGCCGG
GAGCGAAAGCGCTCATCATCGGAACGGATATTTCCCGCTTTTTAGTGGCTGAAGGCGGTGACGCGTTGACGGAGGAATGG
TCCTATGCCGAACCGAGTGCGGGGGCGGGAGCTGTGGCGCTTTTAGTCAGCAGCACCCCTCATGTCTTTTTGCTTGATCA
AGGCGCAAGCGGGTATTACGGGTATGAAGTCATGGATACCTGCCGCCCGATTCCGGATAGTGAAGCGGGGGATGCTGATC
TGTCGCTCATGTCTTATTTGGACTGCTGTGAAAAATCATTTCAGGAATACCGAAAGCGTGTGAGCGGGGCGGATTATAAG
AATACATTTCAATACCTTTCTTTTCATACTCCGTTTGGCGGAATGGTAAAAGGAGCGCATCGGACGATGATGAGAAAATT
GGCAAAGGCTAAGCCGCAAGACATTGAAGCGGATTTTCAGCAACGCGTGCTGCCTGGGGTTCAATACTGTCAGCGCGTCG
GCAACATCATGGGGGCCACCGTATTTTTATCTCTTGCCAGCACGATTGATAACGGCTCTTTTGAAACGCCGAAACGCGTC
GGCTGTTTTTCATACGGTTCGGGCTGCTGCTCTGAATTTTACAGCGGTGTCGTTATGCCGCAAAGCCGGACGCGGCAGAA
TCAATTTCAGATCAGCCGGCATTTGGATGAACGCGTCCGTTTATCGATGGCTGATTATGACCGGCTGCTCCAAGACAGCG
GCATCGTAAAATTCGGCACAAGAAATGCCGCGCCCGCAGCAGACATCCTGAATGCGCATCAAGGAAAAACGCGATTGGTG
CTGACTGAAATAAAAGAGTTTCACAGAAAGTATGAATGGACGACCTAG

Upstream 100 bases:

>100_bases
CCCTGTATTTCACGGCGTTGACAGGCTGCTTGTTGAGTTTTGAACGGAAAAATAAAAGAGGCTTCCACAGGAAGCCTCTT
TACTCTGGGGGCTTGGAGAA

Downstream 100 bases:

>100_bases
TATCAGAAACACTCAGAGCCGGAAAGGCTTGAGTATTTTTAAAGAAACGGAGAATAAACATGAATCCAATGATTGATGTA
CAGGAAACAGAACCGGGCAT

Product: DfnL

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNVGIEAMNVFGGTAYVDVMELAEHRNLDKKRFENLLMKEKVVALPFEDPITFAVNAAKPIIDSLSEEERNQIEMVITCT
ESGIDFGKSISTYVHDYLQLKRNCRLFEVKQACYSLTAGFQMGVNFILSQTSPGAKALIIGTDISRFLVAEGGDALTEEW
SYAEPSAGAGAVALLVSSTPHVFLLDQGASGYYGYEVMDTCRPIPDSEAGDADLSLMSYLDCCEKSFQEYRKRVSGADYK
NTFQYLSFHTPFGGMVKGAHRTMMRKLAKAKPQDIEADFQQRVLPGVQYCQRVGNIMGATVFLSLASTIDNGSFETPKRV
GCFSYGSGCCSEFYSGVVMPQSRTRQNQFQISRHLDERVRLSMADYDRLLQDSGIVKFGTRNAAPAADILNAHQGKTRLV
LTEIKEFHRKYEWTT

Sequences:

>Translated_415_residues
MNVGIEAMNVFGGTAYVDVMELAEHRNLDKKRFENLLMKEKVVALPFEDPITFAVNAAKPIIDSLSEEERNQIEMVITCT
ESGIDFGKSISTYVHDYLQLKRNCRLFEVKQACYSLTAGFQMGVNFILSQTSPGAKALIIGTDISRFLVAEGGDALTEEW
SYAEPSAGAGAVALLVSSTPHVFLLDQGASGYYGYEVMDTCRPIPDSEAGDADLSLMSYLDCCEKSFQEYRKRVSGADYK
NTFQYLSFHTPFGGMVKGAHRTMMRKLAKAKPQDIEADFQQRVLPGVQYCQRVGNIMGATVFLSLASTIDNGSFETPKRV
GCFSYGSGCCSEFYSGVVMPQSRTRQNQFQISRHLDERVRLSMADYDRLLQDSGIVKFGTRNAAPAADILNAHQGKTRLV
LTEIKEFHRKYEWTT
>Mature_415_residues
MNVGIEAMNVFGGTAYVDVMELAEHRNLDKKRFENLLMKEKVVALPFEDPITFAVNAAKPIIDSLSEEERNQIEMVITCT
ESGIDFGKSISTYVHDYLQLKRNCRLFEVKQACYSLTAGFQMGVNFILSQTSPGAKALIIGTDISRFLVAEGGDALTEEW
SYAEPSAGAGAVALLVSSTPHVFLLDQGASGYYGYEVMDTCRPIPDSEAGDADLSLMSYLDCCEKSFQEYRKRVSGADYK
NTFQYLSFHTPFGGMVKGAHRTMMRKLAKAKPQDIEADFQQRVLPGVQYCQRVGNIMGATVFLSLASTIDNGSFETPKRV
GCFSYGSGCCSEFYSGVVMPQSRTRQNQFQISRHLDERVRLSMADYDRLLQDSGIVKFGTRNAAPAADILNAHQGKTRLV
LTEIKEFHRKYEWTT

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI5031751, Length=384, Percent_Identity=24.21875, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI148298764, Length=386, Percent_Identity=24.8704663212435, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI148298677, Length=386, Percent_Identity=24.8704663212435, Blast_Score=88, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323509, Length=459, Percent_Identity=22.4400871459695, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24654139, Length=382, Percent_Identity=24.3455497382199, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24654137, Length=382, Percent_Identity=24.3455497382199, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24654133, Length=382, Percent_Identity=24.3455497382199, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24654135, Length=382, Percent_Identity=24.3455497382199, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17933694, Length=382, Percent_Identity=24.3455497382199, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 46217; Mature: 46217

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVGIEAMNVFGGTAYVDVMELAEHRNLDKKRFENLLMKEKVVALPFEDPITFAVNAAKP
CCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCEEEECCHHH
IIDSLSEEERNQIEMVITCTESGIDFGKSISTYVHDYLQLKRNCRLFEVKQACYSLTAGF
HHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QMGVNFILSQTSPGAKALIIGTDISRFLVAEGGDALTEEWSYAEPSAGAGAVALLVSSTP
HHCCHHEEECCCCCCEEEEEECCHHHHHHCCCCCHHHCCCCCCCCCCCCCEEEEEECCCC
HVFLLDQGASGYYGYEVMDTCRPIPDSEAGDADLSLMSYLDCCEKSFQEYRKRVSGADYK
EEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
NTFQYLSFHTPFGGMVKGAHRTMMRKLAKAKPQDIEADFQQRVLPGVQYCQRVGNIMGAT
HHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VFLSLASTIDNGSFETPKRVGCFSYGSGCCSEFYSGVVMPQSRTRQNQFQISRHLDERVR
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHH
LSMADYDRLLQDSGIVKFGTRNAAPAADILNAHQGKTRLVLTEIKEFHRKYEWTT
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCCCCEEHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVGIEAMNVFGGTAYVDVMELAEHRNLDKKRFENLLMKEKVVALPFEDPITFAVNAAKP
CCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCEEEECCHHH
IIDSLSEEERNQIEMVITCTESGIDFGKSISTYVHDYLQLKRNCRLFEVKQACYSLTAGF
HHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QMGVNFILSQTSPGAKALIIGTDISRFLVAEGGDALTEEWSYAEPSAGAGAVALLVSSTP
HHCCHHEEECCCCCCEEEEEECCHHHHHHCCCCCHHHCCCCCCCCCCCCCEEEEEECCCC
HVFLLDQGASGYYGYEVMDTCRPIPDSEAGDADLSLMSYLDCCEKSFQEYRKRVSGADYK
EEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
NTFQYLSFHTPFGGMVKGAHRTMMRKLAKAKPQDIEADFQQRVLPGVQYCQRVGNIMGAT
HHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VFLSLASTIDNGSFETPKRVGCFSYGSGCCSEFYSGVVMPQSRTRQNQFQISRHLDERVR
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHH
LSMADYDRLLQDSGIVKFGTRNAAPAADILNAHQGKTRLVLTEIKEFHRKYEWTT
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCCCCEEHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]