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The map label for this gene is dfnM [H]

Identifier: 154686625

GI number: 154686625

Start: 2276743

End: 2277489

Strand: Reverse

Name: dfnM [H]

Synonym: RBAM_021940

Alternate gene names: 154686625

Gene position: 2277489-2276743 (Counterclockwise)

Preceding gene: 154686626

Following gene: 154686623

Centisome position: 58.12

GC content: 49.0

Gene sequence:

>747_bases
ATGAATCCAATGATTGATGTACAGGAAACAGAACCGGGCATTGTGCAGGTCACAATGCAGGAGACGGCCGGGAAAAACGG
GCTGTCAGAAGAGTTTGTCAGAGAGCTGCACCGGGTATTCCAAGACATCAATGCCCATCCGTCCTATAAAACCGTCCTTC
TGACGGGTTACGGAAATTATTTCTGCACGGGAGGAACGCAGGAGGGGCTTTTGACCATTCAGAGCGGAAAGCTCCAATTT
ACAGATTTAAAGCTTCACAGTCTTTTATTGGATTGCAGGATCCCTGTGATTTCCGCCATGCAGGGGCATGCGATCGGAGC
CGGCTTTACGATCGGTCTGAGCTCGGATTTTGTGATTTTAAGCAGAGAGAGTTTTTATTCTTGTAATTATATGAAGTACG
GATTTACACCGGGAATGGGCGCGACGTATATTGTGCCTGAAAAACTGGGAATTGTCCTCGGGGAAGAAATGCTGTTTACT
GCCGCGAATTTCAGCGGCGCCGCTCTGGAAAAACGGGGAGTTCCGTTTGAAGTGCTGCCGAAAAAAGATGTCCTTCAAAG
AGGCCGCGAATTAGCCCGGCTGCTGGCGGATAAACCGAGAGCCTCACTGGTTGCGCTGAAAACCAATGTGACGGCACGGA
TCAAAAGAGAGCTGACAAAAGCGGTTGAGGAAGAGGTGAACATGCACGAAGCCACATTCCATCTTCCTGAAGTAAAAGAG
AGAATCAGCCGCTTATACGGCAGTTAA

Upstream 100 bases:

>100_bases
AAATAAAAGAGTTTCACAGAAAGTATGAATGGACGACCTAGTATCAGAAACACTCAGAGCCGGAAAGGCTTGAGTATTTT
TAAAGAAACGGAGAATAAAC

Downstream 100 bases:

>100_bases
ACAGAATGAAAAAAGCATGGGAAGCCCCATGCTTTATCACTGGTCCGTCTTTTGCAGAACGGAGCCTGCGAATTGTTCTT
TAATTTCGGCGCTCCGGGCT

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNPMIDVQETEPGIVQVTMQETAGKNGLSEEFVRELHRVFQDINAHPSYKTVLLTGYGNYFCTGGTQEGLLTIQSGKLQF
TDLKLHSLLLDCRIPVISAMQGHAIGAGFTIGLSSDFVILSRESFYSCNYMKYGFTPGMGATYIVPEKLGIVLGEEMLFT
AANFSGAALEKRGVPFEVLPKKDVLQRGRELARLLADKPRASLVALKTNVTARIKRELTKAVEEEVNMHEATFHLPEVKE
RISRLYGS

Sequences:

>Translated_248_residues
MNPMIDVQETEPGIVQVTMQETAGKNGLSEEFVRELHRVFQDINAHPSYKTVLLTGYGNYFCTGGTQEGLLTIQSGKLQF
TDLKLHSLLLDCRIPVISAMQGHAIGAGFTIGLSSDFVILSRESFYSCNYMKYGFTPGMGATYIVPEKLGIVLGEEMLFT
AANFSGAALEKRGVPFEVLPKKDVLQRGRELARLLADKPRASLVALKTNVTARIKRELTKAVEEEVNMHEATFHLPEVKE
RISRLYGS
>Mature_248_residues
MNPMIDVQETEPGIVQVTMQETAGKNGLSEEFVRELHRVFQDINAHPSYKTVLLTGYGNYFCTGGTQEGLLTIQSGKLQF
TDLKLHSLLLDCRIPVISAMQGHAIGAGFTIGLSSDFVILSRESFYSCNYMKYGFTPGMGATYIVPEKLGIVLGEEMLFT
AANFSGAALEKRGVPFEVLPKKDVLQRGRELARLLADKPRASLVALKTNVTARIKRELTKAVEEEVNMHEATFHLPEVKE
RISRLYGS

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. May have a role in the decarboxylation of the (S)-3-methylglutaryl group attached to pksL [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI116235487, Length=237, Percent_Identity=24.4725738396624, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI194097323, Length=221, Percent_Identity=22.6244343891403, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI221142681, Length=199, Percent_Identity=23.1155778894472, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=195, Percent_Identity=25.6410256410256, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=23.6363636363636, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27476; Mature: 27476

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPMIDVQETEPGIVQVTMQETAGKNGLSEEFVRELHRVFQDINAHPSYKTVLLTGYGNY
CCCCCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCE
FCTGGTQEGLLTIQSGKLQFTDLKLHSLLLDCRIPVISAMQGHAIGAGFTIGLSSDFVIL
EECCCCCCCEEEEECCCEEEEHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCCCEEEE
SRESFYSCNYMKYGFTPGMGATYIVPEKLGIVLGEEMLFTAANFSGAALEKRGVPFEVLP
ECCCCCCCCCEEECCCCCCCCEEECHHHHHHHCCHHHEEEECCCCCCHHHHCCCCEEECC
KKDVLQRGRELARLLADKPRASLVALKTNVTARIKRELTKAVEEEVNMHEATFHLPEVKE
CHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHEEECHHHHH
RISRLYGS
HHHHHHCC
>Mature Secondary Structure
MNPMIDVQETEPGIVQVTMQETAGKNGLSEEFVRELHRVFQDINAHPSYKTVLLTGYGNY
CCCCCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCE
FCTGGTQEGLLTIQSGKLQFTDLKLHSLLLDCRIPVISAMQGHAIGAGFTIGLSSDFVIL
EECCCCCCCEEEEECCCEEEEHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCCCEEEE
SRESFYSCNYMKYGFTPGMGATYIVPEKLGIVLGEEMLFTAANFSGAALEKRGVPFEVLP
ECCCCCCCCCEEECCCCCCCCEEECHHHHHHHCCHHHEEEECCCCCCHHHHCCCCEEECC
KKDVLQRGRELARLLADKPRASLVALKTNVTARIKRELTKAVEEEVNMHEATFHLPEVKE
CHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHEEECHHHHH
RISRLYGS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]