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The map label for this gene is mleA [H]

Identifier: 154686599

GI number: 154686599

Start: 2254859

End: 2256172

Strand: Reverse

Name: mleA [H]

Synonym: RBAM_021680

Alternate gene names: 154686599

Gene position: 2256172-2254859 (Counterclockwise)

Preceding gene: 154686600

Following gene: 154686598

Centisome position: 57.58

GC content: 52.74

Gene sequence:

>1314_bases
ATGATTACAAAGCATATGATTCGGACGTTAATGATTGAAACGCCGACTGTTCCCGGAAATCTCGGAAAAGTGGCAACCGC
CATCGGAGTGATGGGCGGAGATATCGGGGAAGTTGAAACCGTTAAAGTCGGCCCGAACTACACAATGCGCAACATTACGG
TGCAGGCCGAAAATGAAGAGCAGCTGCAGGAAATCATTCAAGCCATTGAAGCTCTCGGTGAAGGCATCCGGCTTCATACA
GTCTCAGATGAAGTGCTCTCTGCTCATGAAGGCGGCAAAATTCAGATGAAAAGCAAAATGCCGATCCGGACACTGGCTGA
GCTTGGGCGTGTTTATACGCCGGGTGTCGCCGATGTGTGCAGACTGATTGAAAACGAGCCTGAAAAAGCGTCCATCTATA
CGACAATCAGCAATTCTGTCGCCATTGTCACCGACGGAACGGCGATTCTCGGCCTCGGCAATATCGGCTCTGTCGCCGGA
ATGCCCGTAATGGAAGGGAAAGCCGCGCTTTTTGACCAGCTGGCGGGTATCAGCGGAATTCCGATTTTGCTCGATACTTC
TGATCCGGAAGAAATCATTCGCACGGTAAAACATATTTCTCCGGGGTTCAGCGGAATTCTGCTTGAAGATATCGGTTCGC
CCCACTGCTTTGACATTGAAGACCGCCTGAAAGAAGAACTGGACATTCCGGTTATGCACGATGACCAGCACGGCACGGCG
GTCGTCACGCTCGCCGCCGCCATTTCCGCTTGTAAAAGCGCCGGTCTTGATTTAAAAGAAGCGAAGGTCGGTCAGATCGG
TCTCGGCGCGGCAGGCGTCGCCATCTGCCGCATGTTTATGGCATACGGAGTCAAAGCGGTCTGCGGAACGGACAAGTCAG
AAGACGCAATGAACCGCCTCGCTCAGTACGGAGGTCAGCCGCTTGGAAGCATCAAAGAATTAATGGACACGTGTGACATT
GTGATCAGCACCACAGGAGTTCCGGGTCTCATTAAAAAAGAGGATGTCAGAAAAGGACACGTCATTCTGGCTTTATCTAA
TCCGAAGCCGGAGATTGAACCGGAAGAGGCATTACAAGCCGGAGCCGCTTATGCAGCTGACGGCCGTTCGGTCAATAATG
TTCTCGGCTTCCCGGGGATTTTCCGCGGCGCGCTGAACGCCAAAAGCAAGAAAATCGACCATGAAATGCTTGTGGCGGCC
GCAGAAGCCATCGCCTCTTGCACAAAACACGGCGATCTCGTGCCTCAGCCTTTGGATGCGGACGTGCATCAAGCCGTATC
AGCAGCCGTAGAACGGGCTGCGGCAGGAAAATAA

Upstream 100 bases:

>100_bases
TATCGGACTGGCAATATTGTACGGATACACGGGGAAATTCATTTGGTACACGAAAGAAAAATCCTTGCAATCTCAAAAAT
TGGGTTAGGAGGTTCATTTC

Downstream 100 bases:

>100_bases
ACAACTCTTACAGTACCGGACATGTGCCGGTGCTGTTTTTTTATCATGAATAGGAAGCGGAATTCTTAGGCAGGCTAACA
GATAGCAAAAGACGGAATAA

Product: MleA

Products: NA

Alternate protein names: NAD-ME 1 [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MITKHMIRTLMIETPTVPGNLGKVATAIGVMGGDIGEVETVKVGPNYTMRNITVQAENEEQLQEIIQAIEALGEGIRLHT
VSDEVLSAHEGGKIQMKSKMPIRTLAELGRVYTPGVADVCRLIENEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAG
MPVMEGKAALFDQLAGISGIPILLDTSDPEEIIRTVKHISPGFSGILLEDIGSPHCFDIEDRLKEELDIPVMHDDQHGTA
VVTLAAAISACKSAGLDLKEAKVGQIGLGAAGVAICRMFMAYGVKAVCGTDKSEDAMNRLAQYGGQPLGSIKELMDTCDI
VISTTGVPGLIKKEDVRKGHVILALSNPKPEIEPEEALQAGAAYAADGRSVNNVLGFPGIFRGALNAKSKKIDHEMLVAA
AEAIASCTKHGDLVPQPLDADVHQAVSAAVERAAAGK

Sequences:

>Translated_437_residues
MITKHMIRTLMIETPTVPGNLGKVATAIGVMGGDIGEVETVKVGPNYTMRNITVQAENEEQLQEIIQAIEALGEGIRLHT
VSDEVLSAHEGGKIQMKSKMPIRTLAELGRVYTPGVADVCRLIENEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAG
MPVMEGKAALFDQLAGISGIPILLDTSDPEEIIRTVKHISPGFSGILLEDIGSPHCFDIEDRLKEELDIPVMHDDQHGTA
VVTLAAAISACKSAGLDLKEAKVGQIGLGAAGVAICRMFMAYGVKAVCGTDKSEDAMNRLAQYGGQPLGSIKELMDTCDI
VISTTGVPGLIKKEDVRKGHVILALSNPKPEIEPEEALQAGAAYAADGRSVNNVLGFPGIFRGALNAKSKKIDHEMLVAA
AEAIASCTKHGDLVPQPLDADVHQAVSAAVERAAAGK
>Mature_437_residues
MITKHMIRTLMIETPTVPGNLGKVATAIGVMGGDIGEVETVKVGPNYTMRNITVQAENEEQLQEIIQAIEALGEGIRLHT
VSDEVLSAHEGGKIQMKSKMPIRTLAELGRVYTPGVADVCRLIENEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAG
MPVMEGKAALFDQLAGISGIPILLDTSDPEEIIRTVKHISPGFSGILLEDIGSPHCFDIEDRLKEELDIPVMHDDQHGTA
VVTLAAAISACKSAGLDLKEAKVGQIGLGAAGVAICRMFMAYGVKAVCGTDKSEDAMNRLAQYGGQPLGSIKELMDTCDI
VISTTGVPGLIKKEDVRKGHVILALSNPKPEIEPEEALQAGAAYAADGRSVNNVLGFPGIFRGALNAKSKKIDHEMLVAA
AEAIASCTKHGDLVPQPLDADVHQAVSAAVERAAAGK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1788806, Length=392, Percent_Identity=37.5, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87081919, Length=432, Percent_Identity=25.462962962963, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 45890; Mature: 45890

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKHMIRTLMIETPTVPGNLGKVATAIGVMGGDIGEVETVKVGPNYTMRNITVQAENEE
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEECCHH
QLQEIIQAIEALGEGIRLHTVSDEVLSAHEGGKIQMKSKMPIRTLAELGRVYTPGVADVC
HHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHH
RLIENEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAGMPVMEGKAALFDQLAGISGI
HHHHCCCCCEEEEEEECCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCC
PILLDTSDPEEIIRTVKHISPGFSGILLEDIGSPHCFDIEDRLKEELDIPVMHDDQHGTA
EEEEECCCHHHHHHHHHHCCCCCCEEEHHHCCCCCEECHHHHHHHHCCCCEEECCCCCHH
VVTLAAAISACKSAGLDLKEAKVGQIGLGAAGVAICRMFMAYGVKAVCGTDKSEDAMNRL
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
AQYGGQPLGSIKELMDTCDIVISTTGVPGLIKKEDVRKGHVILALSNPKPEIEPEEALQA
HHHCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHH
GAAYAADGRSVNNVLGFPGIFRGALNAKSKKIDHEMLVAAAEAIASCTKHGDLVPQPLDA
CCCCCCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
DVHQAVSAAVERAAAGK
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITKHMIRTLMIETPTVPGNLGKVATAIGVMGGDIGEVETVKVGPNYTMRNITVQAENEE
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEECCHH
QLQEIIQAIEALGEGIRLHTVSDEVLSAHEGGKIQMKSKMPIRTLAELGRVYTPGVADVC
HHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHH
RLIENEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAGMPVMEGKAALFDQLAGISGI
HHHHCCCCCEEEEEEECCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCC
PILLDTSDPEEIIRTVKHISPGFSGILLEDIGSPHCFDIEDRLKEELDIPVMHDDQHGTA
EEEEECCCHHHHHHHHHHCCCCCCEEEHHHCCCCCEECHHHHHHHHCCCCEEECCCCCHH
VVTLAAAISACKSAGLDLKEAKVGQIGLGAAGVAICRMFMAYGVKAVCGTDKSEDAMNRL
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
AQYGGQPLGSIKELMDTCDIVISTTGVPGLIKKEDVRKGHVILALSNPKPEIEPEEALQA
HHHCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHH
GAAYAADGRSVNNVLGFPGIFRGALNAKSKKIDHEMLVAAAEAIASCTKHGDLVPQPLDA
CCCCCCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
DVHQAVSAAVERAAAGK
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8501064; 10903309 [H]