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The map label for this gene is mleN [H]

Identifier: 154686600

GI number: 154686600

Start: 2256185

End: 2257591

Strand: Reverse

Name: mleN [H]

Synonym: RBAM_021690

Alternate gene names: 154686600

Gene position: 2257591-2256185 (Counterclockwise)

Preceding gene: 154686601

Following gene: 154686599

Centisome position: 57.61

GC content: 45.63

Gene sequence:

>1407_bases
GTGAAAGAGGTCAGATTGCCAAATTTATTGGAAATTATTATCTTATTATTCGGGTTTTTAGCGCTTGTCTTATCATTCAC
AGTGTTTTTAGACCTGCCGATCCAGCTTGCTCTATTTATATCCTGGTTTATCGCTATTATTCTCGGAGTCAGGCTGGGAC
ATTCATATAAAGACTTGCAGGCTTCGATTGTCACCGGGATATCAAACGGTCTTGAAGCGGTTTTGATTCTTGTTTCGGTC
GGTGCGCTTATCGGGACATGGATTGCCGGCGGCGTTGTGCCGACGTTAATTTATTATGGTTTAGAGTTTATTAATCCTCA
TGTTTTCCTGCTTGCTACATTGGTGATTTGTTCCGTTATGTCCGTTGCGACCGGAACGTCGTGGGGAACCGTGGGGACCG
CGGGGATCGCCATGGTTGCCATCGGAGAAGGAATGGGTATACCGCTTCCGATCGTGGCGGGAGCCGTTTTGTCCGGCGCT
TACTTCGGAGACAAGCTTTCACCGCTGTCTGACAGTACGGTGCTTGCCTCCACGCTTTCTAAGGTGGATGTGCTGTCACA
CGTCAGAGCGATGATGTATCTGTCAATTCCGTCCTATATTATTACGGCCATTCTGTTTACCGTAACGGGATTTATATACG
GCGGAAAAAATATTGATCTTGGCAAAGTGGAATTTTTAAAATCTTCATTGCAGAAAACGTTTGACATTCATATATGGATG
CTCATTCCGGCTGTTGTCGTCGTTGTCCTTCTGATTTTGAAAAAGCCGTCTATGCCCGTTATCGTCATCGGTGCTTTGCT
TGGCGCAATCTGGGCTTCCGTCTTTCAGGGAATGAATTTTGCCAGTGCCATCGCGACTGCTTATAATGGTTTTTCCATTA
AAACGGACGTCGAATTTTTAAACGGCCTGTTAAACCGCGGAGGCATTACCGGTATGCTGGATACGTTAGTCGTTATCCTG
TTCGGTCTTGGTTTTGGAGGAATTCTTGAGAAGCTCGGCGTATTAAAAGTGATTGTCTCAACTTTTGAGAAAAAGCTGAC
CACACCGGGAAATGTGACGCTTTCGACATTGATCGTCGCCCTTTTAGGAAACATTTTCGGCTGTGCGATGTACGTGTCAT
TGATCCTGACGCCGAAAATCATGGAGAACAGTTATGATAAACTGAAACTTGACAGAAGAGTTTTATCCCGGAACGCAGAG
GTGGGCGGCACGCTTACTTCAGGGATGGTGCCGTGGTCTGATAACGGCATCTATATGGCCGGCATTCTCGGCGTATCCAC
ATTTTCTTATCTGCCGTTCATGTGGCTCAGTTTTGTATCTATCGGACTGGCAATATTGTACGGATACACGGGGAAATTCA
TTTGGTACACGAAAGAAAAATCCTTGCAATCTCAAAAATTGGGTTAG

Upstream 100 bases:

>100_bases
AATAGGCGGTTGCCATACAAAGGGATGAGGCAGTATAGTATAATTTGTTATATCATTTGATGAAAGCGTTTTAGCTTTTT
TCTTCTAGGGGGATTTTCTT

Downstream 100 bases:

>100_bases
GAGGTTCATTTCATGATTACAAAGCATATGATTCGGACGTTAATGATTGAAACGCCGACTGTTCCCGGAAATCTCGGAAA
AGTGGCAACCGCCATCGGAG

Product: MleN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKEVRLPNLLEIIILLFGFLALVLSFTVFLDLPIQLALFISWFIAIILGVRLGHSYKDLQASIVTGISNGLEAVLILVSV
GALIGTWIAGGVVPTLIYYGLEFINPHVFLLATLVICSVMSVATGTSWGTVGTAGIAMVAIGEGMGIPLPIVAGAVLSGA
YFGDKLSPLSDSTVLASTLSKVDVLSHVRAMMYLSIPSYIITAILFTVTGFIYGGKNIDLGKVEFLKSSLQKTFDIHIWM
LIPAVVVVVLLILKKPSMPVIVIGALLGAIWASVFQGMNFASAIATAYNGFSIKTDVEFLNGLLNRGGITGMLDTLVVIL
FGLGFGGILEKLGVLKVIVSTFEKKLTTPGNVTLSTLIVALLGNIFGCAMYVSLILTPKIMENSYDKLKLDRRVLSRNAE
VGGTLTSGMVPWSDNGIYMAGILGVSTFSYLPFMWLSFVSIGLAILYGYTGKFIWYTKEKSLQSQKLG

Sequences:

>Translated_468_residues
MKEVRLPNLLEIIILLFGFLALVLSFTVFLDLPIQLALFISWFIAIILGVRLGHSYKDLQASIVTGISNGLEAVLILVSV
GALIGTWIAGGVVPTLIYYGLEFINPHVFLLATLVICSVMSVATGTSWGTVGTAGIAMVAIGEGMGIPLPIVAGAVLSGA
YFGDKLSPLSDSTVLASTLSKVDVLSHVRAMMYLSIPSYIITAILFTVTGFIYGGKNIDLGKVEFLKSSLQKTFDIHIWM
LIPAVVVVVLLILKKPSMPVIVIGALLGAIWASVFQGMNFASAIATAYNGFSIKTDVEFLNGLLNRGGITGMLDTLVVIL
FGLGFGGILEKLGVLKVIVSTFEKKLTTPGNVTLSTLIVALLGNIFGCAMYVSLILTPKIMENSYDKLKLDRRVLSRNAE
VGGTLTSGMVPWSDNGIYMAGILGVSTFSYLPFMWLSFVSIGLAILYGYTGKFIWYTKEKSLQSQKLG
>Mature_468_residues
MKEVRLPNLLEIIILLFGFLALVLSFTVFLDLPIQLALFISWFIAIILGVRLGHSYKDLQASIVTGISNGLEAVLILVSV
GALIGTWIAGGVVPTLIYYGLEFINPHVFLLATLVICSVMSVATGTSWGTVGTAGIAMVAIGEGMGIPLPIVAGAVLSGA
YFGDKLSPLSDSTVLASTLSKVDVLSHVRAMMYLSIPSYIITAILFTVTGFIYGGKNIDLGKVEFLKSSLQKTFDIHIWM
LIPAVVVVVLLILKKPSMPVIVIGALLGAIWASVFQGMNFASAIATAYNGFSIKTDVEFLNGLLNRGGITGMLDTLVVIL
FGLGFGGILEKLGVLKVIVSTFEKKLTTPGNVTLSTLIVALLGNIFGCAMYVSLILTPKIMENSYDKLKLDRRVLSRNAE
VGGTLTSGMVPWSDNGIYMAGILGVSTFSYLPFMWLSFVSIGLAILYGYTGKFIWYTKEKSLQSQKLG

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 50135; Mature: 50135

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]