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The map label for this gene is sigF [H]

Identifier: 154686587

GI number: 154686587

Start: 2246495

End: 2247262

Strand: Reverse

Name: sigF [H]

Synonym: RBAM_021560

Alternate gene names: 154686587

Gene position: 2247262-2246495 (Counterclockwise)

Preceding gene: 154686588

Following gene: 154686586

Centisome position: 57.35

GC content: 45.96

Gene sequence:

>768_bases
ATGGATGTGGAGGTTAAGAAAAACAGCAAAAACGCGCAGCTCAAGGACCATGAAGTCAAAGAATTAATCAAAAAGAGCCA
AGAGGGCGACCAGCAGGCAAGAGACCTCCTCATAGAAAAAAACATGCGTCTTGTGTGGTCCGTCGTTCAAAGGTTCTTAA
ACAGAGGCTATGAGCCGGATGATCTGTTTCAGATCGGCTGTATCGGATTGTTAAAATCCGTTGATAAATTCGATTTGTCC
TATGATGTCCGTTTTTCAACTTATGCCGTGCCGATGATTATCGGAGAAATCCAGCGCTTCATCCGCGATGACGGAACCGT
CAAAGTTTCAAGATCTTTAAAAGAGCTCGGCAATAAAATACGCCGGGCGAAAGATGAACTGTCAAAAACATACGGAAGAG
TGCCGACCGTCCAGGAAATCGCGGACCATTTAGAAATTGATGCGGAGGATGTGGTGCTTGCGCAGGAAGCGGTGCGGGCG
CCTTCATCCATTCATGAGACCGTGTATGAAAATGACGGAGACCCGATCACCCTGCTTGATCAGATCGCCGATCATTCAGA
AGAGAAGTGGTTTGACAAAATCGCCCTCAAGGAGGCGATCAGTGATTTGGAAGAACGGGAAAAACTCATTGTCTATCTGA
GATATTATAAAGATCAGACACAGTCGGAAGTAGCCGAACGGCTCGGGATTTCCCAAGTCCAGGTATCGAGGCTGGAGAAA
AAAATATTAAAGCAGATTAAAGTGCAGATGGATCATACGGAAAGCTGA

Upstream 100 bases:

>100_bases
ATGATGTCACGATTGATTCCTCGCCGGAGATGGGAACGACGATACGCTTAACAAAACACCTATCAAAAAGCAAAGCGCTT
TGTAATTAAGGAGATTTGTT

Downstream 100 bases:

>100_bases
CCTTTTACGGGCCGGCTTTTTTTATTTGTGCATCGGTGGTAATTTATGGTCTTTTGCCGCTGATGAATGAGAACAAAAGT
GAACCGCATACTACATATAT

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MDVEVKKNSKNAQLKDHEVKELIKKSQEGDQQARDLLIEKNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
YDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKIRRAKDELSKTYGRVPTVQEIADHLEIDAEDVVLAQEAVRA
PSSIHETVYENDGDPITLLDQIADHSEEKWFDKIALKEAISDLEEREKLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
KILKQIKVQMDHTES

Sequences:

>Translated_255_residues
MDVEVKKNSKNAQLKDHEVKELIKKSQEGDQQARDLLIEKNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
YDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKIRRAKDELSKTYGRVPTVQEIADHLEIDAEDVVLAQEAVRA
PSSIHETVYENDGDPITLLDQIADHSEEKWFDKIALKEAISDLEEREKLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
KILKQIKVQMDHTES
>Mature_255_residues
MDVEVKKNSKNAQLKDHEVKELIKKSQEGDQQARDLLIEKNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLS
YDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKIRRAKDELSKTYGRVPTVQEIADHLEIDAEDVVLAQEAVRA
PSSIHETVYENDGDPITLLDQIADHSEEKWFDKIALKEAISDLEEREKLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
KILKQIKVQMDHTES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with spoIIAB inhibits sig

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=28.9795918367347, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789098, Length=238, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788231, Length=201, Percent_Identity=29.3532338308458, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789871, Length=241, Percent_Identity=26.5560165975104, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29507; Mature: 29507

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3116160; 6088674; 8969508; 9384377; 8358793 [H]