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The map label for this gene is gudB [H]

Identifier: 154686542

GI number: 154686542

Start: 2207376

End: 2208650

Strand: Reverse

Name: gudB [H]

Synonym: RBAM_021110

Alternate gene names: 154686542

Gene position: 2208650-2207376 (Counterclockwise)

Preceding gene: 154686543

Following gene: 154686541

Centisome position: 56.36

GC content: 46.67

Gene sequence:

>1275_bases
ATGGCAGCCGATCGATTCACCGGTCATAATGAAGAAGACAAACATGATGTATTAAAATCAACCCAAACCGTAATACATAA
AGCTCTGGAAAAATTGGGGTATCCAGAAGAAGTGTATGAACTGCTGAAAGAGCCGCTCAGATTATTAACGGTTAAAATTC
CTGTTCGGATGGATGACGGGTCTGTGAAAATATTTACAGGCTATCGTGCGCAGCACAATGATTCCGTAGGTCCGACAAAA
GGCGGCATTCGTTTTCACCCGAATGTAACTGAAAAAGAGGTGAAAGCACTTTCCATCTGGATGAGTTTAAAATGCGGAAT
TATTGATCTTCCTTATGGCGGTGGTAAAGGCGGAATTGTTTGTGATCCGAGAAATATGTCATTCAGAGAGCTTGAAAGAC
TGAGCAGGGGATATGTCAGAGCAATCAGTCAGATTGTCGGACCGACTAAAGACGTCCCGGCGCCTGACGTATTTACAAAC
TCACAGATTATGGCTTGGATGATGGATGAATATTCAAGAATTGATGAATTTAACTCACCCGGCTTTATTACGGGAAAACC
GCTTGTACTCGGCGGATCCCACGGCAGGGAATCAGCGACGGCAAAAGGCGTCACCATCTGCATAAAAGAAGCGGCCAAGA
AAAAAGGTCTCGATATTCAGAACGCGCGCGTCGTCGTACAAGGTTTCGGAAACGCGGGCAGCTATCTGGCCAAGTTTATG
CATGACGCCGGCGCGAAAGTTGTCGGAATTTCTGACGCGTACGGAGGCCTTTACGATCCGGACGGTCTTGATATTGATTA
TCTGCTTGACCGCAGAGACAGCTTCGGTACGGTGACCAAGCTTTTCAATGACACCATTACGAATCAGGAGCTGCTTGAGC
TGGACTGTGATATACTTGTCCCGGCGGCGATCGAAAACCAAATTACAGAAGAAAACGCTGACCGCATCAAAGCGAAAATC
GTTGTAGAAGCGGCAAACGGACCGACGACACTGGAAGGAACGAAAATCCTGACAGACAAAGGAACGCTGCTGGTTCCCGA
TGTTCTGGCGAGCGCCGGCGGTGTAACGGTTTCTTATTTCGAATGGGTTCAAAACAACCAAGGCTTCTACTGGAGTGAAG
AAGAAGTCGAGGAAAAACTGGAAAATATGATGATAAAATCGTTTAATAATATTTATGAAATGTCCCAAAACCGCCGGATT
GACATGAGGCTTGCGGCTTACATGGTCGGCGTGCGGAAAATGGCGGAGGCTTCGCGTTTCAGAGGCTGGATATAA

Upstream 100 bases:

>100_bases
AAATCATTGTCTTTCTTCTATGCACGGAGATATTGAAGGTGTATACTAGTCACTGAAAGGGAGCATGAGAGAAACAGAAG
ATTTTAGGAGGTTAACTCAA

Downstream 100 bases:

>100_bases
ACCAATCGTTTGCATAAACAAAAAAAATCTCCTATGATAATATAGGAGATTTTTTATATGAGTGGAAACTTATGATGGAA
ATGCCAATCGCAGGAGTTGA

Product: GudB

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAADRFTGHNEEDKHDVLKSTQTVIHKALEKLGYPEEVYELLKEPLRLLTVKIPVRMDDGSVKIFTGYRAQHNDSVGPTK
GGIRFHPNVTEKEVKALSIWMSLKCGIIDLPYGGGKGGIVCDPRNMSFRELERLSRGYVRAISQIVGPTKDVPAPDVFTN
SQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKGLDIQNARVVVQGFGNAGSYLAKFM
HDAGAKVVGISDAYGGLYDPDGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCDILVPAAIENQITEENADRIKAKI
VVEAANGPTTLEGTKILTDKGTLLVPDVLASAGGVTVSYFEWVQNNQGFYWSEEEVEEKLENMMIKSFNNIYEMSQNRRI
DMRLAAYMVGVRKMAEASRFRGWI

Sequences:

>Translated_424_residues
MAADRFTGHNEEDKHDVLKSTQTVIHKALEKLGYPEEVYELLKEPLRLLTVKIPVRMDDGSVKIFTGYRAQHNDSVGPTK
GGIRFHPNVTEKEVKALSIWMSLKCGIIDLPYGGGKGGIVCDPRNMSFRELERLSRGYVRAISQIVGPTKDVPAPDVFTN
SQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKGLDIQNARVVVQGFGNAGSYLAKFM
HDAGAKVVGISDAYGGLYDPDGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCDILVPAAIENQITEENADRIKAKI
VVEAANGPTTLEGTKILTDKGTLLVPDVLASAGGVTVSYFEWVQNNQGFYWSEEEVEEKLENMMIKSFNNIYEMSQNRRI
DMRLAAYMVGVRKMAEASRFRGWI
>Mature_423_residues
AADRFTGHNEEDKHDVLKSTQTVIHKALEKLGYPEEVYELLKEPLRLLTVKIPVRMDDGSVKIFTGYRAQHNDSVGPTKG
GIRFHPNVTEKEVKALSIWMSLKCGIIDLPYGGGKGGIVCDPRNMSFRELERLSRGYVRAISQIVGPTKDVPAPDVFTNS
QIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKGLDIQNARVVVQGFGNAGSYLAKFMH
DAGAKVVGISDAYGGLYDPDGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCDILVPAAIENQITEENADRIKAKIV
VEAANGPTTLEGTKILTDKGTLLVPDVLASAGGVTVSYFEWVQNNQGFYWSEEEVEEKLENMMIKSFNNIYEMSQNRRID
MRLAAYMVGVRKMAEASRFRGWI

Specific function: The wild-type version has little or no activity [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=341, Percent_Identity=41.0557184750733, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI4885281, Length=341, Percent_Identity=40.7624633431085, Blast_Score=272, Evalue=4e-73,
Organism=Escherichia coli, GI1788059, Length=408, Percent_Identity=30.1470588235294, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17544676, Length=337, Percent_Identity=41.5430267062315, Blast_Score=262, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6319256, Length=400, Percent_Identity=28.5, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6324951, Length=408, Percent_Identity=28.1862745098039, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45549226, Length=340, Percent_Identity=40.8823529411765, Blast_Score=268, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24649283, Length=376, Percent_Identity=40.1595744680851, Blast_Score=268, Evalue=4e-72,
Organism=Drosophila melanogaster, GI45553475, Length=340, Percent_Identity=40.8823529411765, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=39.7849462365591, Blast_Score=214, Evalue=9e-56,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 47046; Mature: 46915

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADRFTGHNEEDKHDVLKSTQTVIHKALEKLGYPEEVYELLKEPLRLLTVKIPVRMDDG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCC
SVKIFTGYRAQHNDSVGPTKGGIRFHPNVTEKEVKALSIWMSLKCGIIDLPYGGGKGGIV
CEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHEEEECCEEECCCCCCCCCEE
CDPRNMSFRELERLSRGYVRAISQIVGPTKDVPAPDVFTNSQIMAWMMDEYSRIDEFNSP
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
GFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKGLDIQNARVVVQGFGNAGSYLAKFM
CEECCCCEEEECCCCCCCCCCCCHHEEEHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
HDAGAKVVGISDAYGGLYDPDGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCDILV
HHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEE
PAAIENQITEENADRIKAKIVVEAANGPTTLEGTKILTDKGTLLVPDVLASAGGVTVSYF
CHHHHHHHHHCCHHEEEEEEEEEECCCCCCCCCCEEEECCCCEEEHHHHHCCCCCEEEEH
EWVQNNQGFYWSEEEVEEKLENMMIKSFNNIYEMSQNRRIDMRLAAYMVGVRKMAEASRF
HHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RGWI
CCCC
>Mature Secondary Structure 
AADRFTGHNEEDKHDVLKSTQTVIHKALEKLGYPEEVYELLKEPLRLLTVKIPVRMDDG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCC
SVKIFTGYRAQHNDSVGPTKGGIRFHPNVTEKEVKALSIWMSLKCGIIDLPYGGGKGGIV
CEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHEEEECCEEECCCCCCCCCEE
CDPRNMSFRELERLSRGYVRAISQIVGPTKDVPAPDVFTNSQIMAWMMDEYSRIDEFNSP
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
GFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKGLDIQNARVVVQGFGNAGSYLAKFM
CEECCCCEEEECCCCCCCCCCCCHHEEEHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
HDAGAKVVGISDAYGGLYDPDGLDIDYLLDRRDSFGTVTKLFNDTITNQELLELDCDILV
HHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEE
PAAIENQITEENADRIKAKIVVEAANGPTTLEGTKILTDKGTLLVPDVLASAGGVTVSYF
CHHHHHHHHHCCHHEEEEEEEEEECCCCCCCCCCEEEECCCCEEEHHHHHCCCCCEEEEH
EWVQNNQGFYWSEEEVEEKLENMMIKSFNNIYEMSQNRRIDMRLAAYMVGVRKMAEASRF
HHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RGWI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829940 [H]