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The map label for this gene is ypdA [H]

Identifier: 154686541

GI number: 154686541

Start: 2206287

End: 2207270

Strand: Reverse

Name: ypdA [H]

Synonym: RBAM_021100

Alternate gene names: 154686541

Gene position: 2207270-2206287 (Counterclockwise)

Preceding gene: 154686542

Following gene: 154686540

Centisome position: 56.33

GC content: 47.26

Gene sequence:

>984_bases
ATGACAGAAGAAAAAGCAATTATAATAGGCGGAGGTCCTTGCGGATTATCGGCGGCTATACATCTGAAGCAAATTGGCGT
GAACGCGCTTGTGATCGAAAAAGGGAATGTCGTAAACAGCATTTATAACTATCCGACCCACCAAACTTTTTTCAGTTCAA
GTGAAAAATTGGAGATCGGCGATGTCGCCTTTATTACGGAAAACAGAAAACCGGTCCGAATACAGGCTCTCTCTTACTAC
AGAGAGGTTGTCAGAAGAAAAGAGCTGCGCGTAAACGCCTTTGAAACGGTGGAGCGCGTAACGAAACAGGAAGACGGCCG
TTTTCTTGTAGAAACATCAAAAGACCGCTACCTCACTCCGTTTTGTATCATTGCGACAGGATATTATGATCATCCGAATT
ATATGAACATACCGGGTGAAGGGCTTCCTAAGGTATTTCATTACTTTAAAGAAGGCCATCCTTATTTTGATAAAGATGTT
GCTGTGATCGGCGGGAAAAACTCAAGTGTGGACGCGGCTTTGGAACTGGTGAAGTCCGGCGCGCGAGTCACGGTGCTCTA
CAGAGGACATGAGTACTCCCCGAGCATCAAGCCGTGGATTCTGCCTGAGTTTGAGGCCCTCGTCAGAAACGGCACAATCC
GGATGGAATTCGGCGCTTGTCTTGAAGAAGTGACGGAAGATGAAATCATTTTCCGCTCCGGCCGAAATGAGACGGTCCGG
CTGAAAAACGATTTCGTATTTGCCATGACGGGATACCATCCCGATCACGGTTTCCTCGAAAAAATCGGTGTGCGGATTGA
TGCGGAATCAGGCCGGCCGTTTTTCCGGGAAGAAACGATGGAAACAAATGTTGACGGTGTTTTTATCGCCGGAGTCATTG
CGGCTGGAAATAACGCCAACGAAATTTTTATCGAGAACGGCCGTTTTCACGGCGGCTTGATCGCAAATGAAATATCAAAA
CGGCTGGGTGCAGAGAGCATATGA

Upstream 100 bases:

>100_bases
TCGTTTGCATAAACAAAAAAAATCTCCTATGATAATATAGGAGATTTTTTATATGAGTGGAAACTTATGATGGAAATGCC
AATCGCAGGAGTTGAAGGAC

Downstream 100 bases:

>100_bases
TCATTCATATGCTCTTTTTTAAAAAAATTGAAAGATGTAAAAATATGAACAACATGGTACGATAGATATAGGAATATAAA
GGAAAGAGGTTGCCTGATGT

Product: YpdA

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTEEKAIIIGGGPCGLSAAIHLKQIGVNALVIEKGNVVNSIYNYPTHQTFFSSSEKLEIGDVAFITENRKPVRIQALSYY
REVVRRKELRVNAFETVERVTKQEDGRFLVETSKDRYLTPFCIIATGYYDHPNYMNIPGEGLPKVFHYFKEGHPYFDKDV
AVIGGKNSSVDAALELVKSGARVTVLYRGHEYSPSIKPWILPEFEALVRNGTIRMEFGACLEEVTEDEIIFRSGRNETVR
LKNDFVFAMTGYHPDHGFLEKIGVRIDAESGRPFFREETMETNVDGVFIAGVIAAGNNANEIFIENGRFHGGLIANEISK
RLGAESI

Sequences:

>Translated_327_residues
MTEEKAIIIGGGPCGLSAAIHLKQIGVNALVIEKGNVVNSIYNYPTHQTFFSSSEKLEIGDVAFITENRKPVRIQALSYY
REVVRRKELRVNAFETVERVTKQEDGRFLVETSKDRYLTPFCIIATGYYDHPNYMNIPGEGLPKVFHYFKEGHPYFDKDV
AVIGGKNSSVDAALELVKSGARVTVLYRGHEYSPSIKPWILPEFEALVRNGTIRMEFGACLEEVTEDEIIFRSGRNETVR
LKNDFVFAMTGYHPDHGFLEKIGVRIDAESGRPFFREETMETNVDGVFIAGVIAAGNNANEIFIENGRFHGGLIANEISK
RLGAESI
>Mature_326_residues
TEEKAIIIGGGPCGLSAAIHLKQIGVNALVIEKGNVVNSIYNYPTHQTFFSSSEKLEIGDVAFITENRKPVRIQALSYYR
EVVRRKELRVNAFETVERVTKQEDGRFLVETSKDRYLTPFCIIATGYYDHPNYMNIPGEGLPKVFHYFKEGHPYFDKDVA
VIGGKNSSVDAALELVKSGARVTVLYRGHEYSPSIKPWILPEFEALVRNGTIRMEFGACLEEVTEDEIIFRSGRNETVRL
KNDFVFAMTGYHPDHGFLEKIGVRIDAESGRPFFREETMETNVDGVFIAGVIAAGNNANEIFIENGRFHGGLIANEISKR
LGAESI

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 36680; Mature: 36549

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEKAIIIGGGPCGLSAAIHLKQIGVNALVIEKGNVVNSIYNYPTHQTFFSSSEKLEIG
CCCCCEEEECCCCCCCHHEEEEEECCCEEEEEECCCHHHHHHCCCCCHHHCCCCCCEEEC
DVAFITENRKPVRIQALSYYREVVRRKELRVNAFETVERVTKQEDGRFLVETSKDRYLTP
EEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCEECE
FCIIATGYYDHPNYMNIPGEGLPKVFHYFKEGHPYFDKDVAVIGGKNSSVDAALELVKSG
EEEEEECEECCCCEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCC
ARVTVLYRGHEYSPSIKPWILPEFEALVRNGTIRMEFGACLEEVTEDEIIFRSGRNETVR
CEEEEEEECCCCCCCCCCCCCCCHHHHHHCCEEEEEHHHHHHHCCCCCEEEECCCCCEEE
LKNDFVFAMTGYHPDHGFLEKIGVRIDAESGRPFFREETMETNVDGVFIAGVIAAGNNAN
EECCEEEEEECCCCCCCHHHHHCEEEECCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCC
EIFIENGRFHGGLIANEISKRLGAESI
EEEEECCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TEEKAIIIGGGPCGLSAAIHLKQIGVNALVIEKGNVVNSIYNYPTHQTFFSSSEKLEIG
CCCCEEEECCCCCCCHHEEEEEECCCEEEEEECCCHHHHHHCCCCCHHHCCCCCCEEEC
DVAFITENRKPVRIQALSYYREVVRRKELRVNAFETVERVTKQEDGRFLVETSKDRYLTP
EEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCEECE
FCIIATGYYDHPNYMNIPGEGLPKVFHYFKEGHPYFDKDVAVIGGKNSSVDAALELVKSG
EEEEEECEECCCCEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCC
ARVTVLYRGHEYSPSIKPWILPEFEALVRNGTIRMEFGACLEEVTEDEIIFRSGRNETVR
CEEEEEEECCCCCCCCCCCCCCCHHHHHHCCEEEEEHHHHHHHCCCCCEEEECCCCCEEE
LKNDFVFAMTGYHPDHGFLEKIGVRIDAESGRPFFREETMETNVDGVFIAGVIAAGNNAN
EECCEEEEEECCCCCCCHHHHHCEEEECCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCC
EIFIENGRFHGGLIANEISKRLGAESI
EEEEECCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]