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The map label for this gene is trpA

Identifier: 154686511

GI number: 154686511

Start: 2178010

End: 2178807

Strand: Reverse

Name: trpA

Synonym: RBAM_020790

Alternate gene names: 154686511

Gene position: 2178807-2178010 (Counterclockwise)

Preceding gene: 154686512

Following gene: 154686510

Centisome position: 55.6

GC content: 44.11

Gene sequence:

>798_bases
ATGTTTAATCTGCGAGATTCTGAAAAATTATTTATCCCTTTTATTACGGCTGGCGATCCGCTTCCTGACATTTCTGTTGA
GCTGGCAATATCATTGCAGAATGCAGGCGCCTCTGCCCTTGAAATCGGCGTTCCTTACACAGATCCGCTTGCGGACGGAC
CCGTCATACAACGGGCTTCAAAACGGGCTCTGGAAAACGGAATGAATATCGTAAAAGCAATTGAACTTGGCGGCAAAATG
AAAAAAAACGGCGTTCATATTCCAATTATCCTCTTTACGTATTATAATCCTGTGTTACAATTAGAAAAAGAATACTTTTT
CGCTTTACTGCGGGAAAACGACATAGACGGTCTGCTTGTACCCGACCTTCCATTAGAGGAAAGCGCCCTTTTGCAAATGA
CATGCAAAAAGGAGAACATCGCTTATATCTCACTGGTTGCGCCGACAAGTGAAGACCGTTTGAAAATAATTACTGAACAG
GCCTGCGGCTTTGTGTATTGTGTATCCTCTTTAGGAGTGACGGGCGTACGCAGCGAATTCGATCCGTCTGTATACTCTTT
CATACGTAAGGTGAAAGAATTCAGCTCTGTTCCGGTGGCGGTCGGTTTTGGCATTTCAAACCGCAAACAGGTTGACGGGA
TGAATGAAATATCTGACGGTGTCGTTGTCGGCAGCGCGCTTGTCAAAAAAATTGAAGAGCTTAAAACAAAGCTTGTGAAT
CCCGGAACAAGAGCTGATGCGCTTCTTGAATTTGAAGAATACGCAAAGACGTTCGGACGACTGTACAGTGTAAAATGA

Upstream 100 bases:

>100_bases
GACATGGATAAAGACAAAATCATTCTCGTCAGTTTGTCGGGAAGAGGAGATAAAGATGTTCATACGCTGATGAATGTTTT
GGAAAACGGGGTGGGGACAC

Downstream 100 bases:

>100_bases
GGTGACTGGATTTGCAGATTAAAGAACAGCTGCGGCAGCTGAAACCGTATCAGCCGGGCAAACCAATAGAAGAAGTGAAA
ATGGAATACGGGCTTGAGAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MFNLRDSEKLFIPFITAGDPLPDISVELAISLQNAGASALEIGVPYTDPLADGPVIQRASKRALENGMNIVKAIELGGKM
KKNGVHIPIILFTYYNPVLQLEKEYFFALLRENDIDGLLVPDLPLEESALLQMTCKKENIAYISLVAPTSEDRLKIITEQ
ACGFVYCVSSLGVTGVRSEFDPSVYSFIRKVKEFSSVPVAVGFGISNRKQVDGMNEISDGVVVGSALVKKIEELKTKLVN
PGTRADALLEFEEYAKTFGRLYSVK

Sequences:

>Translated_265_residues
MFNLRDSEKLFIPFITAGDPLPDISVELAISLQNAGASALEIGVPYTDPLADGPVIQRASKRALENGMNIVKAIELGGKM
KKNGVHIPIILFTYYNPVLQLEKEYFFALLRENDIDGLLVPDLPLEESALLQMTCKKENIAYISLVAPTSEDRLKIITEQ
ACGFVYCVSSLGVTGVRSEFDPSVYSFIRKVKEFSSVPVAVGFGISNRKQVDGMNEISDGVVVGSALVKKIEELKTKLVN
PGTRADALLEFEEYAKTFGRLYSVK
>Mature_265_residues
MFNLRDSEKLFIPFITAGDPLPDISVELAISLQNAGASALEIGVPYTDPLADGPVIQRASKRALENGMNIVKAIELGGKM
KKNGVHIPIILFTYYNPVLQLEKEYFFALLRENDIDGLLVPDLPLEESALLQMTCKKENIAYISLVAPTSEDRLKIITEQ
ACGFVYCVSSLGVTGVRSEFDPSVYSFIRKVKEFSSVPVAVGFGISNRKQVDGMNEISDGVVVGSALVKKIEELKTKLVN
PGTRADALLEFEEYAKTFGRLYSVK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=228, Percent_Identity=35.9649122807018, Blast_Score=137, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6321412, Length=226, Percent_Identity=34.9557522123894, Blast_Score=159, Evalue=5e-40,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_BACA2 (A7Z615)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421672.1
- ProteinModelPortal:   A7Z615
- SMR:   A7Z615
- STRING:   A7Z615
- EnsemblBacteria:   EBBACT00000006921
- GeneID:   5460943
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_020790
- eggNOG:   COG0159
- GeneTree:   EBGT00050000001942
- HOGENOM:   HBG391690
- OMA:   VHIPIIL
- ProtClustDB:   PRK13111
- BioCyc:   BAMY326423:RBAM_020790-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29115; Mature: 29115

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 41-41 ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLRDSEKLFIPFITAGDPLPDISVELAISLQNAGASALEIGVPYTDPLADGPVIQRAS
CCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHH
KRALENGMNIVKAIELGGKMKKNGVHIPIILFTYYNPVLQLEKEYFFALLRENDIDGLLV
HHHHHHCHHHHHHHHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEC
PDLPLEESALLQMTCKKENIAYISLVAPTSEDRLKIITEQACGFVYCVSSLGVTGVRSEF
CCCCCCCHHHHEEEECCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCC
DPSVYSFIRKVKEFSSVPVAVGFGISNRKQVDGMNEISDGVVVGSALVKKIEELKTKLVN
CHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHCCEEHHHHHHHHHHHHHHHHCC
PGTRADALLEFEEYAKTFGRLYSVK
CCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFNLRDSEKLFIPFITAGDPLPDISVELAISLQNAGASALEIGVPYTDPLADGPVIQRAS
CCCCCCCCEEEEEEEECCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHH
KRALENGMNIVKAIELGGKMKKNGVHIPIILFTYYNPVLQLEKEYFFALLRENDIDGLLV
HHHHHHCHHHHHHHHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEC
PDLPLEESALLQMTCKKENIAYISLVAPTSEDRLKIITEQACGFVYCVSSLGVTGVRSEF
CCCCCCCHHHHEEEECCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCC
DPSVYSFIRKVKEFSSVPVAVGFGISNRKQVDGMNEISDGVVVGSALVKKIEELKTKLVN
CHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHCCEEHHHHHHHHHHHHHHHHCC
PGTRADALLEFEEYAKTFGRLYSVK
CCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA