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The map label for this gene is cca

Identifier: 154686492

GI number: 154686492

Start: 2161224

End: 2162414

Strand: Reverse

Name: cca

Synonym: RBAM_020600

Alternate gene names: 154686492

Gene position: 2162414-2161224 (Counterclockwise)

Preceding gene: 154686493

Following gene: 154686491

Centisome position: 55.18

GC content: 50.38

Gene sequence:

>1191_bases
ATGGAACAGGAATTTGTCAAAGCCCTCCCCATCCTTCATCACCTGATGAAGGCGGGCCATCAGGCGTACTTTGTCGGGGG
AGCCGTCAGAGACAGCTATATGAAACGAAAAATAGGTGATGTGGACATCGCAACCAGCGCTTCGCCGAATGAGGTGGAGC
GTCTCTTTAAGCGGACTGTCGATGTAGGAAAAGAGCACGGCACCGTCATCGTCCTGTGGGAAGATGAAACCTATGAAGTC
ACCACTTTTCGTGCCGAATCAGAATATAAGGACTACCGGCGCCCTGAAGCTGTGCGGTTCATTACTTCTTTATCAGAAGA
CTTAAAGCGGAGAGACTTAACGATTAATGCGATGGCGATGTCTGCCGAAGGAGAGCTGCTTGATTATTTCGGAGGCGCGC
ATGATATCGGACAAAAGCTGATCCGTACGGTGGGTAACCCTGAAGACCGTTTTCGGGAAGACGCTCTGCGGATGATGAGA
GCCGTCCGCTTTATGAGTCAGCTCGGCTTTCTGTTAGAAAAAGAAACAAGAGAGGCCGTCATAAAAGACAGGGAGCTTTT
GGCACATGTATCAGTAGAACGAAAGACCGTCGAGTTTGAAAAGCTGCTGCAGGGCGCTTCCTCACAAGAAGCGATTCAAA
CGATGGTGCAAACAGGTTTGATACAAGAGCTGCCCGGCCTTTCCGGTTATGAGCAGCAAATGCTGGAAGCGAGCGGCTTT
CCGTTTTCTTCTTTAGATGCACGCGAAGAACGCTGGGCAGCGCTGTTATTGTTTCTCGGTCTTTGTCCGAAAAGCGCCGA
GGCGTTTTTAAAACAATGGAAACTGCCGGGAAAAGTCATAAAAAAAGCGATGCAGATCATAAGCGTTTATCCGGCGCAGC
TGGAAGCGGAAGCGATGTACCGGGCGGGAGAGGCGCTGTTTTCAGCCGTTAAAATCGGGATGCTGAAAAAGCATAAGACG
ATTGATGAACAAAAGCTGAAAGAGGTTCAGAGCCTGTATGAGCAGCTGCCGATCAAAAGCCTTCGAGACCTCGACATTTC
AGGCGCTGATCTGATGGAGCTCAGAAACCGCCGCGCGGGTAAATGGGTGGCCGAAGAACTTCGCCGGATCGAAGAAGCGG
TATTGGCAGGGAAGCTCCCTAACCGAAAACATGACATAAAGGAGTGGCTCGCATCATGGGATCAACATTAA

Upstream 100 bases:

>100_bases
GATCATGCGCTGAAAATGGTGGAGACCGCCTTTTCTTCGCAAAGAATCGTCAGTCAGTATGAACGGATTTATGACGAATT
GGCAGGACCGGAGTGATCTT

Downstream 100 bases:

>100_bases
GAACCCGATTAATTGATTTATTTTCTGAGGCGGGTGACGGCTTTATCTCCGGGCAGAAAATAAGTGACGCGCTCGGCTGC
TCGAGAACCGCCGTTTGGAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MEQEFVKALPILHHLMKAGHQAYFVGGAVRDSYMKRKIGDVDIATSASPNEVERLFKRTVDVGKEHGTVIVLWEDETYEV
TTFRAESEYKDYRRPEAVRFITSLSEDLKRRDLTINAMAMSAEGELLDYFGGAHDIGQKLIRTVGNPEDRFREDALRMMR
AVRFMSQLGFLLEKETREAVIKDRELLAHVSVERKTVEFEKLLQGASSQEAIQTMVQTGLIQELPGLSGYEQQMLEASGF
PFSSLDAREERWAALLLFLGLCPKSAEAFLKQWKLPGKVIKKAMQIISVYPAQLEAEAMYRAGEALFSAVKIGMLKKHKT
IDEQKLKEVQSLYEQLPIKSLRDLDISGADLMELRNRRAGKWVAEELRRIEEAVLAGKLPNRKHDIKEWLASWDQH

Sequences:

>Translated_396_residues
MEQEFVKALPILHHLMKAGHQAYFVGGAVRDSYMKRKIGDVDIATSASPNEVERLFKRTVDVGKEHGTVIVLWEDETYEV
TTFRAESEYKDYRRPEAVRFITSLSEDLKRRDLTINAMAMSAEGELLDYFGGAHDIGQKLIRTVGNPEDRFREDALRMMR
AVRFMSQLGFLLEKETREAVIKDRELLAHVSVERKTVEFEKLLQGASSQEAIQTMVQTGLIQELPGLSGYEQQMLEASGF
PFSSLDAREERWAALLLFLGLCPKSAEAFLKQWKLPGKVIKKAMQIISVYPAQLEAEAMYRAGEALFSAVKIGMLKKHKT
IDEQKLKEVQSLYEQLPIKSLRDLDISGADLMELRNRRAGKWVAEELRRIEEAVLAGKLPNRKHDIKEWLASWDQH
>Mature_396_residues
MEQEFVKALPILHHLMKAGHQAYFVGGAVRDSYMKRKIGDVDIATSASPNEVERLFKRTVDVGKEHGTVIVLWEDETYEV
TTFRAESEYKDYRRPEAVRFITSLSEDLKRRDLTINAMAMSAEGELLDYFGGAHDIGQKLIRTVGNPEDRFREDALRMMR
AVRFMSQLGFLLEKETREAVIKDRELLAHVSVERKTVEFEKLLQGASSQEAIQTMVQTGLIQELPGLSGYEQQMLEASGF
PFSSLDAREERWAALLLFLGLCPKSAEAFLKQWKLPGKVIKKAMQIISVYPAQLEAEAMYRAGEALFSAVKIGMLKKHKT
IDEQKLKEVQSLYEQLPIKSLRDLDISGADLMELRNRRAGKWVAEELRRIEEAVLAGKLPNRKHDIKEWLASWDQH

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=223, Percent_Identity=36.322869955157, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI87081691, Length=236, Percent_Identity=36.4406779661017, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789436, Length=216, Percent_Identity=31.9444444444444, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=219, Percent_Identity=33.7899543378995, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI281360112, Length=215, Percent_Identity=32.5581395348837, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21357337, Length=215, Percent_Identity=32.5581395348837, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_BACA2 (A7Z5Z6)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421653.1
- ProteinModelPortal:   A7Z5Z6
- SMR:   A7Z5Z6
- STRING:   A7Z5Z6
- EnsemblBacteria:   EBBACT00000006970
- GeneID:   5461379
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_020600
- eggNOG:   COG0617
- GeneTree:   EBGT00050000000996
- HOGENOM:   HBG635063
- OMA:   GKASERF
- ProtClustDB:   PRK13299
- BioCyc:   BAMY326423:RBAM_020600-MONOMER
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45259; Mature: 45259

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 30-30 BINDING 111-111 BINDING 154-154 BINDING 157-157 BINDING 160-160 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQEFVKALPILHHLMKAGHQAYFVGGAVRDSYMKRKIGDVDIATSASPNEVERLFKRTV
CCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
DVGKEHGTVIVLWEDETYEVTTFRAESEYKDYRRPEAVRFITSLSEDLKRRDLTINAMAM
HCCCCCCEEEEEECCCCEEEEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEE
SAEGELLDYFGGAHDIGQKLIRTVGNPEDRFREDALRMMRAVRFMSQLGFLLEKETREAV
CCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKDRELLAHVSVERKTVEFEKLLQGASSQEAIQTMVQTGLIQELPGLSGYEQQMLEASGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
PFSSLDAREERWAALLLFLGLCPKSAEAFLKQWKLPGKVIKKAMQIISVYPAQLEAEAMY
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHH
RAGEALFSAVKIGMLKKHKTIDEQKLKEVQSLYEQLPIKSLRDLDISGADLMELRNRRAG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCC
KWVAEELRRIEEAVLAGKLPNRKHDIKEWLASWDQH
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQEFVKALPILHHLMKAGHQAYFVGGAVRDSYMKRKIGDVDIATSASPNEVERLFKRTV
CCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
DVGKEHGTVIVLWEDETYEVTTFRAESEYKDYRRPEAVRFITSLSEDLKRRDLTINAMAM
HCCCCCCEEEEEECCCCEEEEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEE
SAEGELLDYFGGAHDIGQKLIRTVGNPEDRFREDALRMMRAVRFMSQLGFLLEKETREAV
CCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKDRELLAHVSVERKTVEFEKLLQGASSQEAIQTMVQTGLIQELPGLSGYEQQMLEASGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
PFSSLDAREERWAALLLFLGLCPKSAEAFLKQWKLPGKVIKKAMQIISVYPAQLEAEAMY
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHH
RAGEALFSAVKIGMLKKHKTIDEQKLKEVQSLYEQLPIKSLRDLDISGADLMELRNRRAG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCC
KWVAEELRRIEEAVLAGKLPNRKHDIKEWLASWDQH
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA