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The map label for this gene is bioA [H]

Identifier: 154686261

GI number: 154686261

Start: 1916995

End: 1918341

Strand: Reverse

Name: bioA [H]

Synonym: RBAM_018280

Alternate gene names: 154686261

Gene position: 1918341-1916995 (Counterclockwise)

Preceding gene: 154686262

Following gene: 154686260

Centisome position: 48.95

GC content: 49.15

Gene sequence:

>1347_bases
ATGAAACAATCAATGATTGAAAAAAGCAAACAGCATCTTTGGCTGCCTTTCACACAAATGAAAGATTACGATGAAGATCC
GTTAATCATTGAAAGCGGAAATGGGATCATGCTGAAAGACATCAGCGGCAAAGAATATTATGACGGCTTTTCTTCAGTAT
GGCTCAATGTACACGGCCACCGCAAAAAAGAAATGGATGAAGCGATTCAAAAACAGCTTGAAAAAATCGCTCATTCGACC
TTACTGGGTATGACGAACGTTCCCGCAACAGAACTTGCCGAGATGCTGACAGGCATCACACCGGAAAAGCTGACACGCGT
TTTTTACTCCGACAGCGGAGCGGAAGCAATGGAAATCGCTCTGAAAATGGCCTTTCAATACTGGAAAAACATAGGGAGAC
CCGAGAAACAGACGTTTATTTCTATGCAGAACGGCTATCACGGCGATACGATCGGAGCCGTCAGCGTCGGCTCCATTGAA
CTCTTCCACCATGTGTACGGCCCGCTGATGTTTGAAAGCTACAAAGCCCCGATTCCGTATGTATACAGATCTGAAAGCGG
CGACCCCGTACAGTGCCGGGATGAATGCCTTCACGCGCTCGAACAGCTGCTTGCAAAGCATCACGAAGAGATTGCCGCAC
TTACGATCGAATCGATGGTGCAAGGCGCATCCGGGATGATCGTCATGCCTGAAGGCTTTCTGGCAGGCGTACGAAAGCTG
TGTACGGCATATGATGTGTTAATGATCGTTGACGAAGTGGCGACGGGGTTCGGCCGCACCGGAAAAATGTTTGCCTGTGA
GCATGAAAATGTCCAGCCGGACTTGATGGCGGCAGGGAAAGGCATTACGGGAGGCTATTTGCCGATCGCTGTGACGTTTG
CCACAGAAGCCATTTATGAAGCCTTCTACGATGACTACGACAAAATGAAAACATTTTTCCACGGCCATTCCTATACAGGA
AATCAGCTTGGCTGTGCCGCGGCAATTGAAAATCTGCGTTTATTCGAATCAGAGCGCATCGTTGAACAAGTCGCCGAAAA
ATCAAAAACGGCAGCGAAATTTCTTCATGATCTGAAGCAGCTTCCTCATGTCGGCGATGTCCGCCAGCTCGGTTTCATGT
GCGGCATAGAGCTTGTGCAGGATAAGGGGACAAAACAGCCTTATCCGCCTGAAAAGCGGATGGGATACAAGGTGTCCTTG
AAAATGAGAGAGCTTGGTATGCTGACACGGCCGCTCGGAGACGTCATCGCCTTTCTGCCGCCGCTTGCCAGCACAACCGA
TCAGTTACAAGCCATGATCTCCATTATGAAAGAAGCGATAGCCGAGGTGACCGGCCGTGGATTTTGA

Upstream 100 bases:

>100_bases
GCGGATGCCGGATTTTCTTTACAGATGGCTCCATAGATACGGAGAGTTGTATCCATTTTTTGGAGAAGCAACCTGTATTC
ATTCAGCGGGAGGAGAACAT

Downstream 100 bases:

>100_bases
CGGATGGCTCCTCGGAAGGCTCGATGCGGTGAAAAGAGACGGGCTGTATCGGACGCTGCGAACACAGGAAACCGCTCTCA
AAACCAAAGGGCAAAAGCGG

Product: adenosylmethionine--8-amino-7-oxononanoate transaminase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKQSMIEKSKQHLWLPFTQMKDYDEDPLIIESGNGIMLKDISGKEYYDGFSSVWLNVHGHRKKEMDEAIQKQLEKIAHST
LLGMTNVPATELAEMLTGITPEKLTRVFYSDSGAEAMEIALKMAFQYWKNIGRPEKQTFISMQNGYHGDTIGAVSVGSIE
LFHHVYGPLMFESYKAPIPYVYRSESGDPVQCRDECLHALEQLLAKHHEEIAALTIESMVQGASGMIVMPEGFLAGVRKL
CTAYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEAIYEAFYDDYDKMKTFFHGHSYTG
NQLGCAAAIENLRLFESERIVEQVAEKSKTAAKFLHDLKQLPHVGDVRQLGFMCGIELVQDKGTKQPYPPEKRMGYKVSL
KMRELGMLTRPLGDVIAFLPPLASTTDQLQAMISIMKEAIAEVTGRGF

Sequences:

>Translated_448_residues
MKQSMIEKSKQHLWLPFTQMKDYDEDPLIIESGNGIMLKDISGKEYYDGFSSVWLNVHGHRKKEMDEAIQKQLEKIAHST
LLGMTNVPATELAEMLTGITPEKLTRVFYSDSGAEAMEIALKMAFQYWKNIGRPEKQTFISMQNGYHGDTIGAVSVGSIE
LFHHVYGPLMFESYKAPIPYVYRSESGDPVQCRDECLHALEQLLAKHHEEIAALTIESMVQGASGMIVMPEGFLAGVRKL
CTAYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEAIYEAFYDDYDKMKTFFHGHSYTG
NQLGCAAAIENLRLFESERIVEQVAEKSKTAAKFLHDLKQLPHVGDVRQLGFMCGIELVQDKGTKQPYPPEKRMGYKVSL
KMRELGMLTRPLGDVIAFLPPLASTTDQLQAMISIMKEAIAEVTGRGF
>Mature_448_residues
MKQSMIEKSKQHLWLPFTQMKDYDEDPLIIESGNGIMLKDISGKEYYDGFSSVWLNVHGHRKKEMDEAIQKQLEKIAHST
LLGMTNVPATELAEMLTGITPEKLTRVFYSDSGAEAMEIALKMAFQYWKNIGRPEKQTFISMQNGYHGDTIGAVSVGSIE
LFHHVYGPLMFESYKAPIPYVYRSESGDPVQCRDECLHALEQLLAKHHEEIAALTIESMVQGASGMIVMPEGFLAGVRKL
CTAYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEAIYEAFYDDYDKMKTFFHGHSYTG
NQLGCAAAIENLRLFESERIVEQVAEKSKTAAKFLHDLKQLPHVGDVRQLGFMCGIELVQDKGTKQPYPPEKRMGYKVSL
KMRELGMLTRPLGDVIAFLPPLASTTDQLQAMISIMKEAIAEVTGRGF

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=426, Percent_Identity=30.2816901408451, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI13994255, Length=414, Percent_Identity=30.4347826086957, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI284507298, Length=338, Percent_Identity=31.6568047337278, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI37574042, Length=454, Percent_Identity=26.8722466960352, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI226442705, Length=453, Percent_Identity=26.7108167770419, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI226442709, Length=417, Percent_Identity=27.0983213429257, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI24119277, Length=463, Percent_Identity=25.0539956803456, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI188536080, Length=448, Percent_Identity=20.9821428571429, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI38679950, Length=448, Percent_Identity=20.9821428571429, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI38679946, Length=448, Percent_Identity=20.9821428571429, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786991, Length=437, Percent_Identity=35.0114416475973, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI145693181, Length=455, Percent_Identity=30.989010989011, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1788044, Length=430, Percent_Identity=29.0697674418605, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789016, Length=426, Percent_Identity=27.2300469483568, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1789759, Length=425, Percent_Identity=28.2352941176471, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787560, Length=417, Percent_Identity=29.0167865707434, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786349, Length=356, Percent_Identity=25.561797752809, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144271, Length=426, Percent_Identity=27.6995305164319, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32564660, Length=446, Percent_Identity=29.5964125560538, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25144274, Length=303, Percent_Identity=30.03300330033, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71992977, Length=441, Percent_Identity=24.7165532879819, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324386, Length=470, Percent_Identity=28.936170212766, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6324432, Length=431, Percent_Identity=29.0023201856148, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323470, Length=455, Percent_Identity=25.4945054945055, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321456, Length=308, Percent_Identity=26.2987012987013, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357415, Length=427, Percent_Identity=29.5081967213115, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI161085790, Length=453, Percent_Identity=27.1523178807947, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI28574759, Length=456, Percent_Identity=27.1929824561404, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21356575, Length=436, Percent_Identity=27.9816513761468, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62

Molecular weight: Translated: 50026; Mature: 50026

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSMIEKSKQHLWLPFTQMKDYDEDPLIIESGNGIMLKDISGKEYYDGFSSVWLNVHGH
CCHHHHHHHHHCEECCHHHHCCCCCCCEEEECCCEEEEEECCCCHHHCCHHHHEEEECCC
RKKEMDEAIQKQLEKIAHSTLLGMTNVPATELAEMLTGITPEKLTRVFYSDSGAEAMEIA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHH
LKMAFQYWKNIGRPEKQTFISMQNGYHGDTIGAVSVGSIELFHHVYGPLMFESYKAPIPY
HHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCCCE
VYRSESGDPVQCRDECLHALEQLLAKHHEEIAALTIESMVQGASGMIVMPEGFLAGVRKL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHH
CTAYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEAIYE
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHH
AFYDDYDKMKTFFHGHSYTGNQLGCAAAIENLRLFESERIVEQVAEKSKTAAKFLHDLKQ
HHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPHVGDVRQLGFMCGIELVQDKGTKQPYPPEKRMGYKVSLKMRELGMLTRPLGDVIAFLP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEHHHHHHHHHHHHHHHHHHHC
PLASTTDQLQAMISIMKEAIAEVTGRGF
CCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQSMIEKSKQHLWLPFTQMKDYDEDPLIIESGNGIMLKDISGKEYYDGFSSVWLNVHGH
CCHHHHHHHHHCEECCHHHHCCCCCCCEEEECCCEEEEEECCCCHHHCCHHHHEEEECCC
RKKEMDEAIQKQLEKIAHSTLLGMTNVPATELAEMLTGITPEKLTRVFYSDSGAEAMEIA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHH
LKMAFQYWKNIGRPEKQTFISMQNGYHGDTIGAVSVGSIELFHHVYGPLMFESYKAPIPY
HHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCCCE
VYRSESGDPVQCRDECLHALEQLLAKHHEEIAALTIESMVQGASGMIVMPEGFLAGVRKL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHH
CTAYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEAIYE
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHH
AFYDDYDKMKTFFHGHSYTGNQLGCAAAIENLRLFESERIVEQVAEKSKTAAKFLHDLKQ
HHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPHVGDVRQLGFMCGIELVQDKGTKQPYPPEKRMGYKVSLKMRELGMLTRPLGDVIAFLP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEHHHHHHHHHHHHHHHHHHHC
PLASTTDQLQAMISIMKEAIAEVTGRGF
CCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]