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The map label for this gene is lacF [H]

Identifier: 154685650

GI number: 154685650

Start: 1186648

End: 1186962

Strand: Reverse

Name: lacF [H]

Synonym: RBAM_012170

Alternate gene names: 154685650

Gene position: 1186962-1186648 (Counterclockwise)

Preceding gene: 154685651

Following gene: 154685649

Centisome position: 30.29

GC content: 45.71

Gene sequence:

>315_bases
ATGACAAAGGAAGAATTGCAGATGCTCGGTTTTGAAATCGTCGCCTATGCCGGCGACGCCAGAAGCACTTTATTGAAAGT
CCTGAAAGAAGTCCGTGCCGGAAACTTCGAAAACACGGAAGCAGCATTGAAAAGCGCCGATGAAAATTTAACCCTCGCCC
ACAATGCGCAAACGAAAATGTTAGCTGAAGAAGCCGACGGCAAGGACATGGAATTAGGATTTATTTTTGTTCACGGTCAG
GACCACTTGATGACAACCCTTTTGCTCCGTGATCTTATTCAGGATTTTATAGAACTCTACCGAAGCCGCCGATAA

Upstream 100 bases:

>100_bases
GTACAAACTTGTAAGTGAAACAAAAACAATCATTTAACGATCATACATGGTGAAAAGAGTCTCTTTTCATCATGTGTGCC
GTCTTTAGGAGGTTTTTTTC

Downstream 100 bases:

>100_bases
GGAGTGGAAAAATGAACGGTATCATCAAGCAAATCGAAAAGGGAAAACCGTTTTTTGAAAAAATCTCAAGAAACATTTAT
TTAGGCGCGATCCGCGACGG

Product: LacF

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component [H]

Number of amino acids: Translated: 104; Mature: 103

Protein sequence:

>104_residues
MTKEELQMLGFEIVAYAGDARSTLLKVLKEVRAGNFENTEAALKSADENLTLAHNAQTKMLAEEADGKDMELGFIFVHGQ
DHLMTTLLLRDLIQDFIELYRSRR

Sequences:

>Translated_104_residues
MTKEELQMLGFEIVAYAGDARSTLLKVLKEVRAGNFENTEAALKSADENLTLAHNAQTKMLAEEADGKDMELGFIFVHGQ
DHLMTTLLLRDLIQDFIELYRSRR
>Mature_103_residues
TKEELQMLGFEIVAYAGDARSTLLKVLKEVRAGNFENTEAALKSADENLTLAHNAQTKMLAEEADGKDMELGFIFVHGQD
HLMTTLLLRDLIQDFIELYRSRR

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=97, Percent_Identity=36.0824742268041, Blast_Score=66, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 11802; Mature: 11670

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEELQMLGFEIVAYAGDARSTLLKVLKEVRAGNFENTEAALKSADENLTLAHNAQTKM
CCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCHHHH
LAEEADGKDMELGFIFVHGQDHLMTTLLLRDLIQDFIELYRSRR
HHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKEELQMLGFEIVAYAGDARSTLLKVLKEVRAGNFENTEAALKSADENLTLAHNAQTKM
CHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCHHHH
LAEEADGKDMELGFIFVHGQDHLMTTLLLRDLIQDFIELYRSRR
HHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400164; 12397186 [H]