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The map label for this gene is lacE [H]

Identifier: 154685649

GI number: 154685649

Start: 1184939

End: 1186636

Strand: Reverse

Name: lacE [H]

Synonym: RBAM_012160

Alternate gene names: 154685649

Gene position: 1186636-1184939 (Counterclockwise)

Preceding gene: 154685650

Following gene: 154685648

Centisome position: 30.28

GC content: 47.82

Gene sequence:

>1698_bases
ATGAACGGTATCATCAAGCAAATCGAAAAGGGAAAACCGTTTTTTGAAAAAATCTCAAGAAACATTTATTTAGGCGCGAT
CCGCGACGGCTTCTTAACGGCCATGCCTGTTATTTTATTCGCGAGCATTTTCATCCTCACCGCCGCTGTGCCGGAAGTGT
TGGGTTTCACTTGGCCTAAAAGTGTTTCTGAATGGCTTTGGAAAGTTTATAACTATTCAATGGGCGCCCTCGGTCTGATC
GTTGCGGCAACAAGTGCGAAATGCCTGAGTGAATCAATGAACCGAAAAATGCCGAAAAACAAAGGGATCAACCCCGTTTC
AGCGATGCTTGCTTCAGTCGTCGGGTTTCTCATTTTAAGCGTCACTCAAATCGATGGCGGATTCTCCACGGAATACCTCG
GCACAAAAGGTCTTTTAGCTGCTTTTGTATCCGCTTTCATAACAGTGAACGTTTACAGATTCTGCGTGCTGAAAAATATT
ACGATTAAAATGCCTAAAGAAGTACCGGGTACCATTTCACAAACATTTAAAGATGTTTTTCCGTTCGCTTTTTCCGTTTT
GGCCATGGTGATCGTTGATTTATTGATCAGGAATACGCTGAACGTATCTTTTGCAGAGGCTCTCTCCTCTCTCTTATCGC
CGTTTTTTACCGCCGCAGACGGTTATCTGGGGATTTGTGTCATTTGGGGAATGATGGCGCTGTTCTGGTTTGTCGGCGTT
CACGGTCCTTCTATTGTGGAACCGGCCATCGCAGCTATTATTTACGCAAATGTGGAGGCGAACCTTCAGCTCCATAATGC
CGGTGAGCAGGCGTCCAACGTGCTGACCGTCGGACTCGGGAACTTCGTCGGCACAATGGGAGGGACCGGCGCAACCCTTG
TTGTTCCGTTTATCTTTCTTCTTTTCGCGAAATCCAAGCAATTGAAAGCAGTCGGAAAAGCATCCGCGGTTCCGGTGTCT
TTCGCAGTAAACGAACCTCTGCTCTTTGCTGCGCCGATCATTTTAAATCCTTATTTTTTCATTCCGTTTGTTGTAACGCC
GATGGTGAATGTCTGTCTGTTTAAATTTTTCGTGGATGTCATCGGAATGAACAGTTTTATGTATGTTCTTCCGTGGGCCA
CCCCGGCGCCCGCCGGCTTGATTCTCGGAACCGGCATGGGGCTCTCGGCTTTTGTGCTGACGGCTGTATTAATCATTGTC
GATTTCCTGATATACCTGCCATTCTGTAAAGCTTATGACAAAGTATTAGCGGCGAAAGAAGCCCAACACACCGAGCCTGA
AACCGCGCCGCAAACATTACAGCCCGCCATGGCCGGCGCCTCAGAAGACGCGTCCTCCATCATCAGCACAAATCTTCAGG
AATCCTCATCCGCCTTGGAACACAGCACCAATGTGTTAGTGCTGTGCGCCGGAGCGGGAACGAGCGCCATGCTTGCGAAC
GCACTGACAGAAGGAGCCGAAGAATATAACGTGCCGATTACCGCCTCAGCAGGCGCCTACGGATCCCATTACGACATCAT
GAAAGACTTCGATATGATCATTTTGGCTCCGCAGGTCGGCTCTTATTACGATGACATTAAAGAAGATGCGGACCGCCTGC
AAGTGAAACTCATCGCAACGAAAGGAGCGGAATATATCAGTCTCACAAGAAATTCACAAAAAGCCATTCAGTTCATACTG
GAGCATACCTCAAAATAG

Upstream 100 bases:

>100_bases
TTGTTCACGGTCAGGACCACTTGATGACAACCCTTTTGCTCCGTGATCTTATTCAGGATTTTATAGAACTCTACCGAAGC
CGCCGATAAGGAGTGGAAAA

Downstream 100 bases:

>100_bases
AATCGGAGGTTTATCATGGATGCCAAACTTTTGAAATCAAGATTCGAAGACATTTTCAAGAGAAGTAACGGAGAACTGTT
TTTCGCACCCGGCCGGATCA

Product: LacE

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MNGIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPVILFASIFILTAAVPEVLGFTWPKSVSEWLWKVYNYSMGALGLI
VAATSAKCLSESMNRKMPKNKGINPVSAMLASVVGFLILSVTQIDGGFSTEYLGTKGLLAAFVSAFITVNVYRFCVLKNI
TIKMPKEVPGTISQTFKDVFPFAFSVLAMVIVDLLIRNTLNVSFAEALSSLLSPFFTAADGYLGICVIWGMMALFWFVGV
HGPSIVEPAIAAIIYANVEANLQLHNAGEQASNVLTVGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASAVPVS
FAVNEPLLFAAPIILNPYFFIPFVVTPMVNVCLFKFFVDVIGMNSFMYVLPWATPAPAGLILGTGMGLSAFVLTAVLIIV
DFLIYLPFCKAYDKVLAAKEAQHTEPETAPQTLQPAMAGASEDASSIISTNLQESSSALEHSTNVLVLCAGAGTSAMLAN
ALTEGAEEYNVPITASAGAYGSHYDIMKDFDMIILAPQVGSYYDDIKEDADRLQVKLIATKGAEYISLTRNSQKAIQFIL
EHTSK

Sequences:

>Translated_565_residues
MNGIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPVILFASIFILTAAVPEVLGFTWPKSVSEWLWKVYNYSMGALGLI
VAATSAKCLSESMNRKMPKNKGINPVSAMLASVVGFLILSVTQIDGGFSTEYLGTKGLLAAFVSAFITVNVYRFCVLKNI
TIKMPKEVPGTISQTFKDVFPFAFSVLAMVIVDLLIRNTLNVSFAEALSSLLSPFFTAADGYLGICVIWGMMALFWFVGV
HGPSIVEPAIAAIIYANVEANLQLHNAGEQASNVLTVGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASAVPVS
FAVNEPLLFAAPIILNPYFFIPFVVTPMVNVCLFKFFVDVIGMNSFMYVLPWATPAPAGLILGTGMGLSAFVLTAVLIIV
DFLIYLPFCKAYDKVLAAKEAQHTEPETAPQTLQPAMAGASEDASSIISTNLQESSSALEHSTNVLVLCAGAGTSAMLAN
ALTEGAEEYNVPITASAGAYGSHYDIMKDFDMIILAPQVGSYYDDIKEDADRLQVKLIATKGAEYISLTRNSQKAIQFIL
EHTSK
>Mature_565_residues
MNGIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPVILFASIFILTAAVPEVLGFTWPKSVSEWLWKVYNYSMGALGLI
VAATSAKCLSESMNRKMPKNKGINPVSAMLASVVGFLILSVTQIDGGFSTEYLGTKGLLAAFVSAFITVNVYRFCVLKNI
TIKMPKEVPGTISQTFKDVFPFAFSVLAMVIVDLLIRNTLNVSFAEALSSLLSPFFTAADGYLGICVIWGMMALFWFVGV
HGPSIVEPAIAAIIYANVEANLQLHNAGEQASNVLTVGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASAVPVS
FAVNEPLLFAAPIILNPYFFIPFVVTPMVNVCLFKFFVDVIGMNSFMYVLPWATPAPAGLILGTGMGLSAFVLTAVLIIV
DFLIYLPFCKAYDKVLAAKEAQHTEPETAPQTLQPAMAGASEDASSIISTNLQESSSALEHSTNVLVLCAGAGTSAMLAN
ALTEGAEEYNVPITASAGAYGSHYDIMKDFDMIILAPQVGSYYDDIKEDADRLQVKLIATKGAEYISLTRNSQKAIQFIL
EHTSK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=466, Percent_Identity=25.9656652360515, Blast_Score=118, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 60759; Mature: 60759

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS51100 PTS_EIIB_TYPE_3 ; PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1400164; 8277252; 12397186 [H]