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The map label for this gene is aprE [H]

Identifier: 154685484

GI number: 154685484

Start: 1030434

End: 1031582

Strand: Reverse

Name: aprE [H]

Synonym: RBAM_010500

Alternate gene names: 154685484

Gene position: 1031582-1030434 (Counterclockwise)

Preceding gene: 154685486

Following gene: 154685482

Centisome position: 26.33

GC content: 49.17

Gene sequence:

>1149_bases
GTGAGAGGCAAAAAGGTATGGATCAGTTTGCTGTTTGCTTTAGCGTTAATCTTTACGATGGCGTTCGGCAGCACGTCTCC
TGCCCAGGCGGCAGGGAAATCAAACGGGGAAAAGAAATACATTGTCGGATTTAAACAGACAATGAGCACGATGAGCGCCG
CTAAGAAAAAAGATGTCATTTCTGAAAAAGGCGGGAAAGTGCAAAAGCAATTCAAATATGTAGACGCAGCTTCAGCTACA
TTAAATGAAAAAGCTGTAAAAGAGCTGAAAAAAGACCCTAGCGTCGCTTACGTTGAAGAAGATCACGTTGCAAAAGCGTA
CGCGCAGTCCGTGCCTTACGGCGTATCACAAATTAAAGCCCCTGCTCTGCACTCTCAAGGCTTCACCGGATCAAATGTGA
AAGTAGCGGTTATCGACAGCGGTATCGATTCTTCTCATCCTGATTTAAAAGTAGCAGGCGGAGCCAGCATGGTTCCTTCT
GAAACAAATCCTTTCCAAGACTACAACTCTCACGGAACTCACGTTGCCGGTACAGTTGCGGCTCTTAATAACTCAGTCGG
TGTATTAGGCGTTGCGCCAAGCGCATCTCTTTACGCTGTAAAAGTTCTCGGCGCTGACGGTTCCGGCCAGTACAGCTGGA
TCATTAACGGAATTGAGTGGGCGATCGCAAACAATATGGACGTTATTAACATGAGCCTCGGCGGACCTTCTGGTTCTGCA
GCGTTAAAAGCGGCAGTTGACAAAGCCGTTGCTTCCGGCGTCGTAGTCGTAGCGGCAGCCGGTAACGAAGGCACTTCCGG
CGGCTCAAGCACAGTGGGCTACCCTGGTAAATACCCTTCTGTCATTGCGGTAGGCGCTGTTAACAGCAGCAACCAAAGAG
CATCTTTCTCAAGCGTAGGTTCTGAGCTTGATGTCATGGCACCAGGCGTCTCTATCCAAAGCACGCTTCCTGGAAACAAA
TACGGCGCGTACAATGGTACGTCAATGGCATCTCCGCACGTTGCCGGAGCGGCTGCTTTGATTCTTTCTAAGCACCCGAA
CTGGACAAACACTCAAGTCCGCAGCAGTTTAGAAAACACCACTACAAAACTTGGTGATGCTTTCTACTACGGAAAAGGGC
TGATCAACGTACAGGCGGCAGCTCAGTAA

Upstream 100 bases:

>100_bases
AATTGGGTCTACTAAAATATTATTCCATGCTATACAATTAATCCACAGAATAATCTGTCTATTGGTTGTTCTGCAAATGA
AAAAAAGGAGAGGATAAAGA

Downstream 100 bases:

>100_bases
AACATAAAAAAACCGGCGTCGGCCATGGCCCCGCCGGTTTTTTTATTATTTTTCTTCCTCAGCATGTTCAATCCGCTCCA
TGATCGACGGGTGGCTCCCT

Product: hypothetical protein

Products: NA

Alternate protein names: Alkaline protease; Subtilisin DFE; Subtilisin Novo [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MRGKKVWISLLFALALIFTMAFGSTSPAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVISEKGGKVQKQFKYVDAASAT
LNEKAVKELKKDPSVAYVEEDHVAKAYAQSVPYGVSQIKAPALHSQGFTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPS
ETNPFQDYNSHGTHVAGTVAALNNSVGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSA
ALKAAVDKAVASGVVVVAAAGNEGTSGGSSTVGYPGKYPSVIAVGAVNSSNQRASFSSVGSELDVMAPGVSIQSTLPGNK
YGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENTTTKLGDAFYYGKGLINVQAAAQ

Sequences:

>Translated_382_residues
MRGKKVWISLLFALALIFTMAFGSTSPAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVISEKGGKVQKQFKYVDAASAT
LNEKAVKELKKDPSVAYVEEDHVAKAYAQSVPYGVSQIKAPALHSQGFTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPS
ETNPFQDYNSHGTHVAGTVAALNNSVGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSA
ALKAAVDKAVASGVVVVAAAGNEGTSGGSSTVGYPGKYPSVIAVGAVNSSNQRASFSSVGSELDVMAPGVSIQSTLPGNK
YGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENTTTKLGDAFYYGKGLINVQAAAQ
>Mature_382_residues
MRGKKVWISLLFALALIFTMAFGSTSPAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVISEKGGKVQKQFKYVDAASAT
LNEKAVKELKKDPSVAYVEEDHVAKAYAQSVPYGVSQIKAPALHSQGFTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPS
ETNPFQDYNSHGTHVAGTVAALNNSVGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSA
ALKAAVDKAVASGVVVVAAAGNEGTSGGSSTVGYPGKYPSVIAVGAVNSSNQRASFSSVGSELDVMAPGVSIQSTLPGNK
YGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENTTTKLGDAFYYGKGLINVQAAAQ

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=256, Percent_Identity=32.03125, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI31317307, Length=300, Percent_Identity=31.6666666666667, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI186972143, Length=279, Percent_Identity=31.1827956989247, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI20336188, Length=376, Percent_Identity=24.468085106383, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI27894285, Length=376, Percent_Identity=24.468085106383, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI20336190, Length=376, Percent_Identity=24.468085106383, Blast_Score=66, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71983555, Length=261, Percent_Identity=27.2030651340996, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25141268, Length=261, Percent_Identity=27.2030651340996, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17533391, Length=252, Percent_Identity=31.3492063492063, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988308, Length=264, Percent_Identity=28.4090909090909, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71987206, Length=283, Percent_Identity=27.9151943462898, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71988301, Length=264, Percent_Identity=28.4090909090909, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71988312, Length=264, Percent_Identity=28.4090909090909, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71988315, Length=264, Percent_Identity=28.4090909090909, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71988321, Length=264, Percent_Identity=28.4090909090909, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71988297, Length=264, Percent_Identity=28.4090909090909, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324576, Length=294, Percent_Identity=32.9931972789116, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320775, Length=317, Percent_Identity=34.7003154574132, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6319893, Length=235, Percent_Identity=34.8936170212766, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324091, Length=274, Percent_Identity=30.2919708029197, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550681, Length=300, Percent_Identity=30.3333333333333, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17137360, Length=257, Percent_Identity=33.0739299610895, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24653270, Length=257, Percent_Identity=33.0739299610895, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17136192, Length=281, Percent_Identity=28.1138790035587, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 39144; Mature: 39144

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGKKVWISLLFALALIFTMAFGSTSPAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVI
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
SEKGGKVQKQFKYVDAASATLNEKAVKELKKDPSVAYVEEDHVAKAYAQSVPYGVSQIKA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCHHHHCC
PALHSQGFTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETNPFQDYNSHGTHVAGTVA
CCHHCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHH
ALNNSVGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSA
HHCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEEECEEEEEECCCEEEEEECCCCCCHH
ALKAAVDKAVASGVVVVAAAGNEGTSGGSSTVGYPGKYPSVIAVGAVNSSNQRASFSSVG
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHCC
SELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENT
CCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHH
TTKLGDAFYYGKGLINVQAAAQ
HHHHCCEEEECCCEEEEEECCC
>Mature Secondary Structure
MRGKKVWISLLFALALIFTMAFGSTSPAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVI
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
SEKGGKVQKQFKYVDAASATLNEKAVKELKKDPSVAYVEEDHVAKAYAQSVPYGVSQIKA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCHHHHCC
PALHSQGFTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETNPFQDYNSHGTHVAGTVA
CCHHCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHH
ALNNSVGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSA
HHCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEEECEEEEEECCCEEEEEECCCCCCHH
ALKAAVDKAVASGVVVVAAAGNEGTSGGSSTVGYPGKYPSVIAVGAVNSSNQRASFSSVG
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHCC
SELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENT
CCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHH
TTKLGDAFYYGKGLINVQAAAQ
HHHHCCEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6090391; 6316278; 6065094; 12524032; 4399039; 6387152; 2684274; 5249818 [H]