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The map label for this gene is phoA [H]

Identifier: 154685401

GI number: 154685401

Start: 945785

End: 947164

Strand: Reverse

Name: phoA [H]

Synonym: RBAM_009670

Alternate gene names: 154685401

Gene position: 947164-945785 (Counterclockwise)

Preceding gene: 154685403

Following gene: 154685399

Centisome position: 24.17

GC content: 48.84

Gene sequence:

>1380_bases
ATGAGTCTATTTAAACAGGTTCGATCAAAACTTCTGCCGGCTGCAGCCGTATCCGTGCTGACAGCCGGTCTGATTGCGGG
TGCGGGGCTTCAGCACTCCGAACAGGCTGCGGCAAAAAAACAGCCTGACAAAAACGAAATCAGAAACGTCATCTTCATGG
TCGGCGACGGAATGGGCGCTCCCTATATGAACGCCTACCGTTCGGTCATTAACAAAGGAAAGCCTTCGGATGGTATGAAA
CTGACGGCATTCGATCCCAATCTCACCGGTATGATGATGACTTATCCTGACGATCCTGATTCCAATATTACCGATTCCGC
GGCGGCTGGCACGGCAATGGCCACGGGCGTGAAAACGTATAACGCGGCGATCGGAGTCGATAAAAAAGGAAAGCGGGTAA
AATCCGTCCTCGAAGAAGCGAAACAAAGAGGGAAATCCACCGGACTTGTCGCAACTTCGGAGATTGCCCACGCCACCCCT
GCGGCATTCGGAGCCCATAACAAATCCCGAAAGAACATGGATGACATCGCAAGCAGCTACTTTGATGACCGCATCAACGG
AAAACAGAAAGTCGATGTGCTCCTCGGAGGCGGCAAGTTAAACTTTATCCGTAAAGACCGCAATGTAACAAAGGATTTCA
AAAAAGCGGGATACAGCTACGTGACATCAAAACAGGAGCTCAAAAAGGATCGTAACAAGAAGATTCTCGGCCTGTTTGCC
GATGGCGGACTCGACAAAGCAATCGACCGCTCAAACGCCGTCCCTTCATTAGAAGACATGACAACGGCAGCGATCGACAG
GCTGAACAAGAATAAAAAAGGGTTTTTCTTAATGGTCGAAGGCAGCCAAATTGACTGGGCGGGACATGACAATGATATAG
TCGGCGCAATGAGTGAAATGAAGGATTTTGAAAAAGCGTATAAAGCAGCGATCGCATTTGCCAAAAAGGACAAACATACG
CTTGTCGTTGCCACGGCTGACCATACCACCGGCGGATTGACGATCGGCGCGAATGACGATAAAAACTGGCATGTTGAACC
GATTCTTGCCGCAAAAAAAACTCCGGCATTTATGGCTGACCAAATCAAAGCGGGCAAATCCGTATCTTCCGTGCTTGCGC
GCTATACAGACCTGAAATGGTCAAAGGCGGAAATCAAACAGATCGAAACGGCCGCAAAACAGGATGCTGACAAGAGCATC
TACAAAGCAATCATTAAGCTCTTTAATGCGAAAACCAACAGCGGCTGGACAACCGGCGATCATACAGGCGAAGAAGTTCC
CGTTTACGCATACGGTCCGGGAAGAGAGTCATTTACGGGGCTTTTAAACAACACAGACCAGGCCAAAAAGATCTTTCACA
TCCTGAAACACGGAAAATAA

Upstream 100 bases:

>100_bases
TAACAATGTTTCACCTGAAATTTACAGGAGGAATAGGCGCTCTTTACAAACACCCCCTACCATTAGACATGCAGACACGA
TTAAAGGAGGACGAGTGAGA

Downstream 100 bases:

>100_bases
AAAAAGGATGCGGGGGTTTCCCCGCATCCTTTCTTCTTTTACAGATATCTCCGGTGCACTCCTTTGCCGTCATACGAAAA
CATCACTTCTCTGTCTTCAA

Product: PhoA

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSLFKQVRSKLLPAAAVSVLTAGLIAGAGLQHSEQAAAKKQPDKNEIRNVIFMVGDGMGAPYMNAYRSVINKGKPSDGMK
LTAFDPNLTGMMMTYPDDPDSNITDSAAAGTAMATGVKTYNAAIGVDKKGKRVKSVLEEAKQRGKSTGLVATSEIAHATP
AAFGAHNKSRKNMDDIASSYFDDRINGKQKVDVLLGGGKLNFIRKDRNVTKDFKKAGYSYVTSKQELKKDRNKKILGLFA
DGGLDKAIDRSNAVPSLEDMTTAAIDRLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMKDFEKAYKAAIAFAKKDKHT
LVVATADHTTGGLTIGANDDKNWHVEPILAAKKTPAFMADQIKAGKSVSSVLARYTDLKWSKAEIKQIETAAKQDADKSI
YKAIIKLFNAKTNSGWTTGDHTGEEVPVYAYGPGRESFTGLLNNTDQAKKIFHILKHGK

Sequences:

>Translated_459_residues
MSLFKQVRSKLLPAAAVSVLTAGLIAGAGLQHSEQAAAKKQPDKNEIRNVIFMVGDGMGAPYMNAYRSVINKGKPSDGMK
LTAFDPNLTGMMMTYPDDPDSNITDSAAAGTAMATGVKTYNAAIGVDKKGKRVKSVLEEAKQRGKSTGLVATSEIAHATP
AAFGAHNKSRKNMDDIASSYFDDRINGKQKVDVLLGGGKLNFIRKDRNVTKDFKKAGYSYVTSKQELKKDRNKKILGLFA
DGGLDKAIDRSNAVPSLEDMTTAAIDRLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMKDFEKAYKAAIAFAKKDKHT
LVVATADHTTGGLTIGANDDKNWHVEPILAAKKTPAFMADQIKAGKSVSSVLARYTDLKWSKAEIKQIETAAKQDADKSI
YKAIIKLFNAKTNSGWTTGDHTGEEVPVYAYGPGRESFTGLLNNTDQAKKIFHILKHGK
>Mature_458_residues
SLFKQVRSKLLPAAAVSVLTAGLIAGAGLQHSEQAAAKKQPDKNEIRNVIFMVGDGMGAPYMNAYRSVINKGKPSDGMKL
TAFDPNLTGMMMTYPDDPDSNITDSAAAGTAMATGVKTYNAAIGVDKKGKRVKSVLEEAKQRGKSTGLVATSEIAHATPA
AFGAHNKSRKNMDDIASSYFDDRINGKQKVDVLLGGGKLNFIRKDRNVTKDFKKAGYSYVTSKQELKKDRNKKILGLFAD
GGLDKAIDRSNAVPSLEDMTTAAIDRLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMKDFEKAYKAAIAFAKKDKHTL
VVATADHTTGGLTIGANDDKNWHVEPILAAKKTPAFMADQIKAGKSVSSVLARYTDLKWSKAEIKQIETAAKQDADKSIY
KAIIKLFNAKTNSGWTTGDHTGEEVPVYAYGPGRESFTGLLNNTDQAKKIFHILKHGK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=356, Percent_Identity=32.5842696629214, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI94721246, Length=351, Percent_Identity=29.6296296296296, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI157266296, Length=348, Percent_Identity=30.1724137931034, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI116734717, Length=356, Percent_Identity=28.9325842696629, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI294660770, Length=347, Percent_Identity=28.5302593659942, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI294660772, Length=302, Percent_Identity=29.1390728476821, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI48994877, Length=463, Percent_Identity=30.4535637149028, Blast_Score=156, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320689, Length=487, Percent_Identity=31.4168377823409, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21355981, Length=465, Percent_Identity=29.4623655913978, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI18859923, Length=417, Percent_Identity=27.8177458033573, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21355151, Length=450, Percent_Identity=28, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24585213, Length=366, Percent_Identity=30.0546448087432, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921912, Length=473, Percent_Identity=28.5412262156448, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355149, Length=353, Percent_Identity=32.0113314447592, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24651554, Length=341, Percent_Identity=34.0175953079179, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI85725200, Length=357, Percent_Identity=29.6918767507003, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI85815833, Length=357, Percent_Identity=29.6918767507003, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21358067, Length=455, Percent_Identity=27.032967032967, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24651556, Length=301, Percent_Identity=33.2225913621262, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24657835, Length=470, Percent_Identity=26.3829787234043, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24657827, Length=489, Percent_Identity=26.1758691206544, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24657842, Length=471, Percent_Identity=25.0530785562633, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 49675; Mature: 49544

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFKQVRSKLLPAAAVSVLTAGLIAGAGLQHSEQAAAKKQPDKNEIRNVIFMVGDGMGA
CCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHCEEEEEEECCCCC
PYMNAYRSVINKGKPSDGMKLTAFDPNLTGMMMTYPDDPDSNITDSAAAGTAMATGVKTY
HHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCHHHCCHHHHHCCHHH
NAAIGVDKKGKRVKSVLEEAKQRGKSTGLVATSEIAHATPAAFGAHNKSRKNMDDIASSY
HHEECCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHCCCHHHCCCCCCCCCHHHHHHHH
FDDRINGKQKVDVLLGGGKLNFIRKDRNVTKDFKKAGYSYVTSKQELKKDRNKKILGLFA
HHHHCCCCEEEEEEEECCEEEEEECCCCHHHHHHHCCCHHHCCHHHHHHCCCCEEEEEEE
DGGLDKAIDRSNAVPSLEDMTTAAIDRLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEM
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCHHHHHHHHH
KDFEKAYKAAIAFAKKDKHTLVVATADHTTGGLTIGANDDKNWHVEPILAAKKTPAFMAD
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCEEECEEHHCCCCCHHHHH
QIKAGKSVSSVLARYTDLKWSKAEIKQIETAAKQDADKSIYKAIIKLFNAKTNSGWTTGD
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HTGEEVPVYAYGPGRESFTGLLNNTDQAKKIFHILKHGK
CCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLFKQVRSKLLPAAAVSVLTAGLIAGAGLQHSEQAAAKKQPDKNEIRNVIFMVGDGMGA
CHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHCEEEEEEECCCCC
PYMNAYRSVINKGKPSDGMKLTAFDPNLTGMMMTYPDDPDSNITDSAAAGTAMATGVKTY
HHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCHHHCCHHHHHCCHHH
NAAIGVDKKGKRVKSVLEEAKQRGKSTGLVATSEIAHATPAAFGAHNKSRKNMDDIASSY
HHEECCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHCCCHHHCCCCCCCCCHHHHHHHH
FDDRINGKQKVDVLLGGGKLNFIRKDRNVTKDFKKAGYSYVTSKQELKKDRNKKILGLFA
HHHHCCCCEEEEEEEECCEEEEEECCCCHHHHHHHCCCHHHCCHHHHHHCCCCEEEEEEE
DGGLDKAIDRSNAVPSLEDMTTAAIDRLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEM
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCHHHHHHHHH
KDFEKAYKAAIAFAKKDKHTLVVATADHTTGGLTIGANDDKNWHVEPILAAKKTPAFMAD
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCEEECEEHHCCCCCHHHHH
QIKAGKSVSSVLARYTDLKWSKAEIKQIETAAKQDADKSIYKAIIKLFNAKTNSGWTTGD
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HTGEEVPVYAYGPGRESFTGLLNNTDQAKKIFHILKHGK
CCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]