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The map label for this gene is opuE [H]

Identifier: 154685161

GI number: 154685161

Start: 687067

End: 688545

Strand: Reverse

Name: opuE [H]

Synonym: RBAM_007060

Alternate gene names: 154685161

Gene position: 688545-687067 (Counterclockwise)

Preceding gene: 154685165

Following gene: 154685160

Centisome position: 17.57

GC content: 49.9

Gene sequence:

>1479_bases
GTGAGTATTGAACTAATTATATCTCTTGGCATTTACTTTGCTGCCATGCTGTTAATCGGCTGGTACGCATTCAGGAAGAC
AACGAATATTAATGATTACATGCTGGGCGGAAGAGGACTCGGCCCGTTCGTAACGGCGCTGTCCGCAGGTGCGGCCGATA
TGAGCGCATGGATGCTGATGGGAGTTCCCGGCGCAATGTTCGCAACGGGGTTATCGACCTTATGGCTGGCGCTCGGTTTG
ACGATCGGCGCGTATTCAAATTATCTGCTGCTTGCTCCGAGGCTGCGCGCTTACACGGAAGTTGCGGATGATGCGATTAC
GATTCCCGATTTTTTCGATAAACGGTTCCGCCACTCGTCATCATTGCTGAAAATTGTTTCCGCCGTGATTATTATGATCT
TTTTCACACTGTACACATCGTCCGGGATGGTATCCGGAGGCAGGTTATTTGAATCGGCATTCGGCGCCGACTACAAATTC
GGTTTGTTTTTAACGGCCGGTGTTGTTGTGCTTTACACGCTGTTTGGCGGTTTCCTCGCTGTCAGTCTGACTGACTTTGT
ACAGGGAGCGATCATGTTTGCGGCATTAGTGCTTGTGCCGATCGTTGCTTTTACCCAGCTCGGGAGCGTTTCAACGACGA
TTGATTCGATCAACGCCGTTAACCCGAAACTATTGGATATCTTTAAAGGCGCGAGCGTCATTAGCATTATTTCTTATTTG
GCATGGGGACTCGGCTATTACGGCCAGCCTCATATCATCGTACGATTTATGGCGATTAAAGAAGTGAAAGATTTAAAACC
CGCCCGCAGAATCGGGATGAGCTGGATGGCCATTTCAGTTGTCGGTTCATTGCTTACCGGGATTATCGGGGTTGCGTATT
CTCATCACTTCGGAGTCTCCGTAAAAGACCCGGAAACGATTTTCATTATTTTTTCTAAAATCTTGTTCCACCCGCTGATT
ACGGGCTTCTTGCTGTCCGCGATTTTAGCGGCGATCATGAGTTCCATTTCCTCACAGCTTCTCGTAACGGCAAGCGCCAT
TACGGAAGATTTATACCGCGCTTTTTTCAGACGGGAAGCGAGCGATAAAGAATTAGTCATGACCGGACGCCTGTCGGTAT
TGATCGTGGCCGTTATCGCCATCCTGCTTTCCATGAATCCGAACAGCACCATTCTTGATCTGGTCGGATATGCGTGGGCA
GGTTTCGGTTCGGCTATCGGCCCGGCGCTCTTGCTCAGTCTGTACTGGAAACGGATGAATGAATGGGGAGCGCTTGCGGC
GATGATTACCGGGGCCGCGGCCGTTTTAATCTGGATCACGACAGGGCTTGCCGCTTCAACCGGCGTATACGAAATCATTC
CGGGGTTCTTCCTCAGTCTGATTGCCGGTATTGCCGTCAGCCTGCTGACCAAAAAACCGGCCGATGCTTCTTATCAGCTG
TTCAGCCGGATGGAAGCTCTTTTGAAAAACAAGAAATAG

Upstream 100 bases:

>100_bases
GGAATGACGACTAAAGGCTGGATCGCCGGCGCATATTCCGGCACTACTAATCTACAGAGGTGATGCAACATTTACATGAA
AGAAGAGAGGGTCCAAAAAC

Downstream 100 bases:

>100_bases
ACAAAAAGCCGTCAGATTTTATCCCTGACGGCTTTTTTCTTCCGCACTTGTCCTGCTATAAATACTGTGAGAAAATCATA
TCAATTTATTATAAGAAAAA

Product: OpuE

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSIELIISLGIYFAAMLLIGWYAFRKTTNINDYMLGGRGLGPFVTALSAGAADMSAWMLMGVPGAMFATGLSTLWLALGL
TIGAYSNYLLLAPRLRAYTEVADDAITIPDFFDKRFRHSSSLLKIVSAVIIMIFFTLYTSSGMVSGGRLFESAFGADYKF
GLFLTAGVVVLYTLFGGFLAVSLTDFVQGAIMFAALVLVPIVAFTQLGSVSTTIDSINAVNPKLLDIFKGASVISIISYL
AWGLGYYGQPHIIVRFMAIKEVKDLKPARRIGMSWMAISVVGSLLTGIIGVAYSHHFGVSVKDPETIFIIFSKILFHPLI
TGFLLSAILAAIMSSISSQLLVTASAITEDLYRAFFRREASDKELVMTGRLSVLIVAVIAILLSMNPNSTILDLVGYAWA
GFGSAIGPALLLSLYWKRMNEWGALAAMITGAAAVLIWITTGLAASTGVYEIIPGFFLSLIAGIAVSLLTKKPADASYQL
FSRMEALLKNKK

Sequences:

>Translated_492_residues
MSIELIISLGIYFAAMLLIGWYAFRKTTNINDYMLGGRGLGPFVTALSAGAADMSAWMLMGVPGAMFATGLSTLWLALGL
TIGAYSNYLLLAPRLRAYTEVADDAITIPDFFDKRFRHSSSLLKIVSAVIIMIFFTLYTSSGMVSGGRLFESAFGADYKF
GLFLTAGVVVLYTLFGGFLAVSLTDFVQGAIMFAALVLVPIVAFTQLGSVSTTIDSINAVNPKLLDIFKGASVISIISYL
AWGLGYYGQPHIIVRFMAIKEVKDLKPARRIGMSWMAISVVGSLLTGIIGVAYSHHFGVSVKDPETIFIIFSKILFHPLI
TGFLLSAILAAIMSSISSQLLVTASAITEDLYRAFFRREASDKELVMTGRLSVLIVAVIAILLSMNPNSTILDLVGYAWA
GFGSAIGPALLLSLYWKRMNEWGALAAMITGAAAVLIWITTGLAASTGVYEIIPGFFLSLIAGIAVSLLTKKPADASYQL
FSRMEALLKNKK
>Mature_491_residues
SIELIISLGIYFAAMLLIGWYAFRKTTNINDYMLGGRGLGPFVTALSAGAADMSAWMLMGVPGAMFATGLSTLWLALGLT
IGAYSNYLLLAPRLRAYTEVADDAITIPDFFDKRFRHSSSLLKIVSAVIIMIFFTLYTSSGMVSGGRLFESAFGADYKFG
LFLTAGVVVLYTLFGGFLAVSLTDFVQGAIMFAALVLVPIVAFTQLGSVSTTIDSINAVNPKLLDIFKGASVISIISYLA
WGLGYYGQPHIIVRFMAIKEVKDLKPARRIGMSWMAISVVGSLLTGIIGVAYSHHFGVSVKDPETIFIIFSKILFHPLIT
GFLLSAILAAIMSSISSQLLVTASAITEDLYRAFFRREASDKELVMTGRLSVLIVAVIAILLSMNPNSTILDLVGYAWAG
FGSAIGPALLLSLYWKRMNEWGALAAMITGAAAVLIWITTGLAASTGVYEIIPGFFLSLIAGIAVSLLTKKPADASYQLF
SRMEALLKNKK

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=476, Percent_Identity=56.9327731092437, Blast_Score=534, Evalue=1e-152,
Organism=Homo sapiens, GI14140236, Length=549, Percent_Identity=23.4972677595628, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI109659839, Length=518, Percent_Identity=23.3590733590734, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI109659836, Length=504, Percent_Identity=23.8095238095238, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787251, Length=490, Percent_Identity=48.7755102040816, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI87082237, Length=453, Percent_Identity=30.4635761589404, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1790503, Length=439, Percent_Identity=27.1070615034169, Blast_Score=132, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI115533094, Length=413, Percent_Identity=24.2130750605327, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24648033, Length=452, Percent_Identity=22.5663716814159, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356865, Length=452, Percent_Identity=22.5663716814159, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24651739, Length=473, Percent_Identity=20.9302325581395, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 52991; Mature: 52859

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCC
>Mature Secondary Structure 
SIELIISLGIYFAAMLLIGWYAFRKTTNINDYMLGGRGLGPFVTALSAGAADMSAWMLM
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH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HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]