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The map label for this gene is cotA [H]

Identifier: 154685125

GI number: 154685125

Start: 643858

End: 645396

Strand: Reverse

Name: cotA [H]

Synonym: RBAM_006700

Alternate gene names: 154685125

Gene position: 645396-643858 (Counterclockwise)

Preceding gene: 154685126

Following gene: 154685124

Centisome position: 16.47

GC content: 50.49

Gene sequence:

>1539_bases
ATGGCACTGGAAAAATTTGCAGATGAACTGCCGATTATCGAAACACTCCAGCCGCAGAAGAAATCAGACGGCAGCACGTA
TTATGAAGTCACCATGAAGGAATGCTTTCACAAGCTGCACCGCGATCTCCCGCCGACCCGGCTGTGGGGGTATAACGGTT
TATTTCCCGGCCCGACGATCGACGTGAACAAAGATGAAAACGTCTATATTAAATGGATGAATGACCTGCCGGATAAGCAT
TTTCTGCCCGTAGATCATACCATTCACCATTCAGAAAGCGGCCATCAGGAACCCGATGTCAAAACCGTCGTCCATTTACA
CGGAGGAGCAACGCCGCCGGACAGCGACGGCTACCCGGAAGCATGGTTCACAAAGGATTTTAAGGAAACGGGTCCTTATT
TTGAGAGAGAGGTATACCATTATCCAAACAAACAGCGCGGGGCTCTGTTATGGTATCACGATCACGCCATGGCGATTACG
AGGCTCAATGTGTACGCCGGGCTTGCCGGGATGTATATCATTCGCGAGCGAAAAGAAAAGCAGCTGAAGCTTCCCTCCGG
AGAGTATGACGTACCGCTTATGATTTTGGACCGCACATTAAATGACGACGGTTCCTTGTCTTATCCGAGCGGACCTGCTA
ACCCTTCCGAAACGCTGCCCGACCCTTCCATCGTCCCGTTTTTTTGCGGAAATACCATTCTCGTCAATGGTAAAGCGTGG
CCGTATATGGAAGTTGAACCTCGGACATATCGTTTCCGCATCCTTAATGCCTCCAATACGAGAACATTTTCTCTCTCGCT
CAATAACGGCGGGAAGTTTATCCAAATCGGTTCTGACGGCGGACTGCTTCCCCGCTCTGTCAAAACACAATCTATCAGCT
TAGCCCCGGCAGAGCGGTATGATGTGCTCATCGATTTCTCCGCCTTTGACGGAGAACATATCATTTTAACGAACGGCACC
GGCTGCGGGGGCGACGCCGATCCGGATACAGACGCCAATGTGATGCAATTCCGCGTCACAAAACCGCTGAAGGGAGCAGA
CACAAGCCGGAAGCCTAAATATCTTTCAACAATGCCTAATGTGACATCAAAGAGAATACACAATATCAGGATGCTTAAAC
TCACAAATACGCAAGACAAATACGGCCGGCCCGTTTTGACGCTGAATAACAAGCGATGGCATGATCCTGTGACAGAAGCG
CCGAAGCTCGGCACAACGGAAATCTGGTCGATTATCAATCCGATGGGGGGAACCCATCCGATACACCTGCACCTTGTTTC
CTTCCAAATCCTTGACCGGCGCCCTTTTGACTTAGAGCGTTATAACAAATTCGGCGACATTGTGTATACAGGCCCCGCCG
TCCCGCCTCCTCCAAGTGAGAAAGGCTGGAAAGATACCGTGCAGGCGCACTCGGGAGAGGTCATCAGAATCGCGGCGACC
TTCGCTCCTTACAGCGGACGCTACGTATGGCACTGTCATATTTTAGAACATGAAGATTACGACATGATGAGACCGATGGA
CGTCACGGATAAGCAGTAA

Upstream 100 bases:

>100_bases
CACCCCCCTCAAAAAGGGCGGGTGTTTTTTTAACCAACCTGTTCCAGGCAGCATTAACTGTAGTACAGGATTTCAATAAA
TGATAAGGACAGGATGAAAA

Downstream 100 bases:

>100_bases
ACAAATATCACCCCTTTTAAAGAAGGGGATTTTTTATTTATCCAAAAAGAAAAAATAAGAAGACACTTATTTGCATATAC
TGTAATAACAGGAAGACGAT

Product: CotA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MALEKFADELPIIETLQPQKKSDGSTYYEVTMKECFHKLHRDLPPTRLWGYNGLFPGPTIDVNKDENVYIKWMNDLPDKH
FLPVDHTIHHSESGHQEPDVKTVVHLHGGATPPDSDGYPEAWFTKDFKETGPYFEREVYHYPNKQRGALLWYHDHAMAIT
RLNVYAGLAGMYIIRERKEKQLKLPSGEYDVPLMILDRTLNDDGSLSYPSGPANPSETLPDPSIVPFFCGNTILVNGKAW
PYMEVEPRTYRFRILNASNTRTFSLSLNNGGKFIQIGSDGGLLPRSVKTQSISLAPAERYDVLIDFSAFDGEHIILTNGT
GCGGDADPDTDANVMQFRVTKPLKGADTSRKPKYLSTMPNVTSKRIHNIRMLKLTNTQDKYGRPVLTLNNKRWHDPVTEA
PKLGTTEIWSIINPMGGTHPIHLHLVSFQILDRRPFDLERYNKFGDIVYTGPAVPPPPSEKGWKDTVQAHSGEVIRIAAT
FAPYSGRYVWHCHILEHEDYDMMRPMDVTDKQ

Sequences:

>Translated_512_residues
MALEKFADELPIIETLQPQKKSDGSTYYEVTMKECFHKLHRDLPPTRLWGYNGLFPGPTIDVNKDENVYIKWMNDLPDKH
FLPVDHTIHHSESGHQEPDVKTVVHLHGGATPPDSDGYPEAWFTKDFKETGPYFEREVYHYPNKQRGALLWYHDHAMAIT
RLNVYAGLAGMYIIRERKEKQLKLPSGEYDVPLMILDRTLNDDGSLSYPSGPANPSETLPDPSIVPFFCGNTILVNGKAW
PYMEVEPRTYRFRILNASNTRTFSLSLNNGGKFIQIGSDGGLLPRSVKTQSISLAPAERYDVLIDFSAFDGEHIILTNGT
GCGGDADPDTDANVMQFRVTKPLKGADTSRKPKYLSTMPNVTSKRIHNIRMLKLTNTQDKYGRPVLTLNNKRWHDPVTEA
PKLGTTEIWSIINPMGGTHPIHLHLVSFQILDRRPFDLERYNKFGDIVYTGPAVPPPPSEKGWKDTVQAHSGEVIRIAAT
FAPYSGRYVWHCHILEHEDYDMMRPMDVTDKQ
>Mature_511_residues
ALEKFADELPIIETLQPQKKSDGSTYYEVTMKECFHKLHRDLPPTRLWGYNGLFPGPTIDVNKDENVYIKWMNDLPDKHF
LPVDHTIHHSESGHQEPDVKTVVHLHGGATPPDSDGYPEAWFTKDFKETGPYFEREVYHYPNKQRGALLWYHDHAMAITR
LNVYAGLAGMYIIRERKEKQLKLPSGEYDVPLMILDRTLNDDGSLSYPSGPANPSETLPDPSIVPFFCGNTILVNGKAWP
YMEVEPRTYRFRILNASNTRTFSLSLNNGGKFIQIGSDGGLLPRSVKTQSISLAPAERYDVLIDFSAFDGEHIILTNGTG
CGGDADPDTDANVMQFRVTKPLKGADTSRKPKYLSTMPNVTSKRIHNIRMLKLTNTQDKYGRPVLTLNNKRWHDPVTEAP
KLGTTEIWSIINPMGGTHPIHLHLVSFQILDRRPFDLERYNKFGDIVYTGPAVPPPPSEKGWKDTVQAHSGEVIRIAATF
APYSGRYVWHCHILEHEDYDMMRPMDVTDKQ

Specific function: Involved in brown pigmentation during sporogenesis [H]

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To S.antibioticus phenoxazinone synthase (phsA) [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=509, Percent_Identity=25.5402750491159, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789394, Length=442, Percent_Identity=27.6018099547511, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR008972 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 58146; Mature: 58014

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEKFADELPIIETLQPQKKSDGSTYYEVTMKECFHKLHRDLPPTRLWGYNGLFPGPTI
CCHHHHHHCCCCHHHCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
DVNKDENVYIKWMNDLPDKHFLPVDHTIHHSESGHQEPDVKTVVHLHGGATPPDSDGYPE
ECCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
AWFTKDFKETGPYFEREVYHYPNKQRGALLWYHDHAMAITRLNVYAGLAGMYIIRERKEK
HHHCCCHHHCCCHHHHHHHCCCCCCCCEEEEEECCEEEEEEEHHHHHHHHHEEEECCCHH
QLKLPSGEYDVPLMILDRTLNDDGSLSYPSGPANPSETLPDPSIVPFFCGNTILVNGKAW
HEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCC
PYMEVEPRTYRFRILNASNTRTFSLSLNNGGKFIQIGSDGGLLPRSVKTQSISLAPAERY
CEEEECCCEEEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEECCCCCE
DVLIDFSAFDGEHIILTNGTGCGGDADPDTDANVMQFRVTKPLKGADTSRKPKYLSTMPN
EEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCC
VTSKRIHNIRMLKLTNTQDKYGRPVLTLNNKRWHDPVTEAPKLGTTEIWSIINPMGGTHP
HHHHHHCEEEEEEEECCCHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
IHLHLVSFQILDRRPFDLERYNKFGDIVYTGPAVPPPPSEKGWKDTVQAHSGEVIRIAAT
EEEEEEEEEEECCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHCCCCEEEEEEE
FAPYSGRYVWHCHILEHEDYDMMRPMDVTDKQ
ECCCCCCEEEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALEKFADELPIIETLQPQKKSDGSTYYEVTMKECFHKLHRDLPPTRLWGYNGLFPGPTI
CHHHHHHCCCCHHHCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
DVNKDENVYIKWMNDLPDKHFLPVDHTIHHSESGHQEPDVKTVVHLHGGATPPDSDGYPE
ECCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
AWFTKDFKETGPYFEREVYHYPNKQRGALLWYHDHAMAITRLNVYAGLAGMYIIRERKEK
HHHCCCHHHCCCHHHHHHHCCCCCCCCEEEEEECCEEEEEEEHHHHHHHHHEEEECCCHH
QLKLPSGEYDVPLMILDRTLNDDGSLSYPSGPANPSETLPDPSIVPFFCGNTILVNGKAW
HEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCC
PYMEVEPRTYRFRILNASNTRTFSLSLNNGGKFIQIGSDGGLLPRSVKTQSISLAPAERY
CEEEECCCEEEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEECCCCCE
DVLIDFSAFDGEHIILTNGTGCGGDADPDTDANVMQFRVTKPLKGADTSRKPKYLSTMPN
EEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCC
VTSKRIHNIRMLKLTNTQDKYGRPVLTLNNKRWHDPVTEAPKLGTTEIWSIINPMGGTHP
HHHHHHCEEEEEEEECCCHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
IHLHLVSFQILDRRPFDLERYNKFGDIVYTGPAVPPPPSEKGWKDTVQAHSGEVIRIAAT
EEEEEEEEEEECCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHCCCCEEEEEEE
FAPYSGRYVWHCHILEHEDYDMMRPMDVTDKQ
ECCCCCCEEEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969499; 9455482; 9384377; 2821284; 3135411 [H]