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The map label for this gene is yclM [H]

Identifier: 154684873

GI number: 154684873

Start: 401812

End: 403176

Strand: Reverse

Name: yclM [H]

Synonym: RBAM_004040

Alternate gene names: 154684873

Gene position: 403176-401812 (Counterclockwise)

Preceding gene: 154684878

Following gene: 154684872

Centisome position: 10.29

GC content: 51.21

Gene sequence:

>1365_bases
ATGAAAGTTGTAAAATTCGGAGGCAGCTCTCTTGCGTCAGGAGCACAGCTTGAGAAAGTGTTTCAAATCGTGACGGCTGA
TCCCGCGCGGAAAGCCGTCGTCGTATCGGCACCGGGAAAACGCTTCTCGGACGATACAAAGGTGACGGATCTGTTAATCC
GCTGTGCGGAGCATTATTTGCAAAAAGGTGTAGCGCCCGAGCTTGCCGAAACGGTCATTGAACGGTACGCCGCCATCGCG
CGTGAGCTGAACCTGGATTTCTCTATTATAGAAAGAATCCGCAGTGACCTATTCGCTCTTTTAAACAGCTGTAAAACCAA
TCCCGAACAATATATGGATGCGGTGAAGGCGAGCGGCGAGGACAACAACGCGAAGCTGATCGCAGCCTACTTCCGACGGC
AGGGCGTGAAAGCGGAATACGTAAGTCCGAAAGAAGCGGGGCTGTTTGTGACGGATGAACCCGGAAGCGCGCAGGTGCTC
CCTGAGTCTTATGGTCATTTGTACCGTCTGAGAAACCGTGAAGGATTGATCATTTTTCCGGGATTTTTCGGTTACACGAA
GGAGCATGAGATTGTGACGTTTTCCCGGAGCGGTTCTGATATTACCGGCTCCATTCTCGCCAGCGGCTTAAAAGCCGACC
TGTACGAAAACTTTACTGATGTGGATGCCGTCTATTCCGTCAACCCGTCAATCGTGCGGCATCCGAAAGAAATTAACGAA
CTGACGTATCGGGAAATGCGTGAGCTCTCCTATGCCGGATTTTCCGTTTTTCATGATGAAGCGCTGATTCCCGCATTTCG
GGCCGGCATCCCCGTTCAAATTAAGAATACGAATAATCCGGATGCGAAAGGAACACGCGTCGTCAGCAGCCGTGACAATA
CGAACGGGCCAGTCATCGGCATCGCAAGCGACACGGGCTTTTGCAGCATTTACATAAGCAAATATTTAATGAACCGGGAA
ATCGGTTTCGGCCGCAAAGCCCTTCACATTTTGGAGGAAGAAGGATTGACGTATGAACACGTTCCATCCGGCATTGACGA
CATCACGATCATCCTTCGGCACAATCAGATGGACTCAGAACTGGAACACAGGCTCGTCACCCGTCTGAAGCAGGAACTTC
AAGCAGATGAAGTCACAGTCAGGCACAATCTGGCGCTGATTATGGTCGTCGGGGAAGCGATGCGCCATAATGTCGGCACA
ACCGCCAGGGCATCCAAAGCACTGTCGGAAGCCGGCGTCAACATCGAGATGATCAATCAAGGGTCATCGGAAGTCAGCAT
GATGTTCGGAGTGAAAGAAGAACAGGAAAAACACGCGGTGCGGGCACTGTATGACGAATTTTTCGCCGGAGTCTTACTCT
CATAG

Upstream 100 bases:

>100_bases
TGTTCTCCCCACGTAGAAAAAATCCTTGATTTACATGTTTGAAAGAGAGATAATAAAAATTAAATTTGTGAGAAAATTTA
AATATTAGGGGATGTAAATC

Downstream 100 bases:

>100_bases
GACACCGCCTCTTTTTTCTCTTGTCCACTAATATCCCTTACAAAGCAGCTTCTTTTACATATTGTATGGTATAGACAATA
TGGAGGTGAAGCTCATGTCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKVVKFGGSSLASGAQLEKVFQIVTADPARKAVVVSAPGKRFSDDTKVTDLLIRCAEHYLQKGVAPELAETVIERYAAIA
RELNLDFSIIERIRSDLFALLNSCKTNPEQYMDAVKASGEDNNAKLIAAYFRRQGVKAEYVSPKEAGLFVTDEPGSAQVL
PESYGHLYRLRNREGLIIFPGFFGYTKEHEIVTFSRSGSDITGSILASGLKADLYENFTDVDAVYSVNPSIVRHPKEINE
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPDAKGTRVVSSRDNTNGPVIGIASDTGFCSIYISKYLMNRE
IGFGRKALHILEEEGLTYEHVPSGIDDITIILRHNQMDSELEHRLVTRLKQELQADEVTVRHNLALIMVVGEAMRHNVGT
TARASKALSEAGVNIEMINQGSSEVSMMFGVKEEQEKHAVRALYDEFFAGVLLS

Sequences:

>Translated_454_residues
MKVVKFGGSSLASGAQLEKVFQIVTADPARKAVVVSAPGKRFSDDTKVTDLLIRCAEHYLQKGVAPELAETVIERYAAIA
RELNLDFSIIERIRSDLFALLNSCKTNPEQYMDAVKASGEDNNAKLIAAYFRRQGVKAEYVSPKEAGLFVTDEPGSAQVL
PESYGHLYRLRNREGLIIFPGFFGYTKEHEIVTFSRSGSDITGSILASGLKADLYENFTDVDAVYSVNPSIVRHPKEINE
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPDAKGTRVVSSRDNTNGPVIGIASDTGFCSIYISKYLMNRE
IGFGRKALHILEEEGLTYEHVPSGIDDITIILRHNQMDSELEHRLVTRLKQELQADEVTVRHNLALIMVVGEAMRHNVGT
TARASKALSEAGVNIEMINQGSSEVSMMFGVKEEQEKHAVRALYDEFFAGVLLS
>Mature_454_residues
MKVVKFGGSSLASGAQLEKVFQIVTADPARKAVVVSAPGKRFSDDTKVTDLLIRCAEHYLQKGVAPELAETVIERYAAIA
RELNLDFSIIERIRSDLFALLNSCKTNPEQYMDAVKASGEDNNAKLIAAYFRRQGVKAEYVSPKEAGLFVTDEPGSAQVL
PESYGHLYRLRNREGLIIFPGFFGYTKEHEIVTFSRSGSDITGSILASGLKADLYENFTDVDAVYSVNPSIVRHPKEINE
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPDAKGTRVVSSRDNTNGPVIGIASDTGFCSIYISKYLMNRE
IGFGRKALHILEEEGLTYEHVPSGIDDITIILRHNQMDSELEHRLVTRLKQELQADEVTVRHNLALIMVVGEAMRHNVGT
TARASKALSEAGVNIEMINQGSSEVSMMFGVKEEQEKHAVRALYDEFFAGVLLS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=474, Percent_Identity=27.2151898734177, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790455, Length=471, Percent_Identity=26.3269639065817, Blast_Score=114, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6320893, Length=502, Percent_Identity=22.3107569721116, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50305; Mature: 50305

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVKFGGSSLASGAQLEKVFQIVTADPARKAVVVSAPGKRFSDDTKVTDLLIRCAEHYL
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
QKGVAPELAETVIERYAAIARELNLDFSIIERIRSDLFALLNSCKTNPEQYMDAVKASGE
HCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DNNAKLIAAYFRRQGVKAEYVSPKEAGLFVTDEPGSAQVLPESYGHLYRLRNREGLIIFP
CCCCHHHEEHHHHCCCCEEECCCCCCCEEEECCCCCCEECHHHHHHHEEECCCCCEEEEC
GFFGYTKEHEIVTFSRSGSDITGSILASGLKADLYENFTDVDAVYSVNPSIVRHPKEINE
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCHHHHCCCHHHHH
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPDAKGTRVVSSRDNTNGPVIG
HHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCCCEEE
IASDTGFCSIYISKYLMNREIGFGRKALHILEEEGLTYEHVPSGIDDITIILRHNQMDSE
EECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCHH
LEHRLVTRLKQELQADEVTVRHNLALIMVVGEAMRHNVGTTARASKALSEAGVNIEMINQ
HHHHHHHHHHHHHHHCCEEEHHCEEEEEEECHHHHCCCCCHHHHHHHHHHCCCEEEEECC
GSSEVSMMFGVKEEQEKHAVRALYDEFFAGVLLS
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVVKFGGSSLASGAQLEKVFQIVTADPARKAVVVSAPGKRFSDDTKVTDLLIRCAEHYL
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
QKGVAPELAETVIERYAAIARELNLDFSIIERIRSDLFALLNSCKTNPEQYMDAVKASGE
HCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DNNAKLIAAYFRRQGVKAEYVSPKEAGLFVTDEPGSAQVLPESYGHLYRLRNREGLIIFP
CCCCHHHEEHHHHCCCCEEECCCCCCCEEEECCCCCCEECHHHHHHHEEECCCCCEEEEC
GFFGYTKEHEIVTFSRSGSDITGSILASGLKADLYENFTDVDAVYSVNPSIVRHPKEINE
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCHHHHCCCHHHHH
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPDAKGTRVVSSRDNTNGPVIG
HHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCCCEEE
IASDTGFCSIYISKYLMNREIGFGRKALHILEEEGLTYEHVPSGIDDITIILRHNQMDSE
EECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCHH
LEHRLVTRLKQELQADEVTVRHNLALIMVVGEAMRHNVGTTARASKALSEAGVNIEMINQ
HHHHHHHHHHHHHHHCCEEEHHCEEEEEEECHHHHCCCCCHHHHHHHHHHCCCEEEEECC
GSSEVSMMFGVKEEQEKHAVRALYDEFFAGVLLS
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]