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The map label for this gene is sfp [H]

Identifier: 154684843

GI number: 154684843

Start: 373164

End: 373838

Strand: Reverse

Name: sfp [H]

Synonym: RBAM_003740

Alternate gene names: 154684843

Gene position: 373838-373164 (Counterclockwise)

Preceding gene: 154684844

Following gene: 154684841

Centisome position: 9.54

GC content: 52.3

Gene sequence:

>675_bases
ATGAAAATTTACGGAGTATATATGGACCGCCCGCTTTCTGCAGGGGAAGAGGATCGGATGATGGCGGCCGTGTCCGCCGA
AAAGCGGGAAAAATGCCGGCGCTTTTACCATAAGGAGGATGCTCACCGCACCTTGATCGGCGACATGCTGATCCGCACCG
CTGCGGCGAAGGCTTATGGACTTGATCCGGCCGGGATTTCATTCGGCGTCCAGGAATACGGAAAGCCGTACATCCCCGCG
CTTCCGGACATGCACTTTAACATTTCCCACTCCGGGCGCTGGATCGTGTGCGCCGTTGATTCAAAACCGATCGGCATTGA
TATTGAAAAAATGAAGCCCGGCACAATTGATATCGCCAAACGGTTTTTTTCGCCGACGGAATACAGTGATCTGCAAGCGA
AACACCCCGATCAGCAGACCGATTATTTTTACCACCTGTGGTCGATGAAAGAAAGCTTTATCAAGCAGGCCGGAAAAGGG
CTTTCCCTGCCGCTTGATTCATTCAGCGTCCGCCTTAAAGACGACGGCCATGTGTCCATTGAGCTCCCGGACGGACATGA
ACCTTGTTTCATCCGCACATATGAGGCGGACGAGGAGTATAAGCTGGCCGTTTGTGCGGCGCATCCCGATTTTTGTGACG
GGATTGAGATGAAAACGTATGAAGAGCTGTTATAA

Upstream 100 bases:

>100_bases
AAAAACAAAGCCGAAACGGTTTTGTTTTTTTATTTTCGGACAGCTTTCGTCTGATATGATAGGATGGTTTTGACAATATT
TTCAGACGGAGGATCTGGAC

Downstream 100 bases:

>100_bases
GCAATCCGTCAGCATTTTGATGCCTCGCGTGATATCCCCTGTCTTGACGTTGGACACGTTGAGTTTGATGATATTTTCTT
TCGGAAAATCCGATAGGTAA

Product: Sfp

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: Lipopeptide antibiotics iturin A and surfactin biosynthesis protein [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKIYGVYMDRPLSAGEEDRMMAAVSAEKREKCRRFYHKEDAHRTLIGDMLIRTAAAKAYGLDPAGISFGVQEYGKPYIPA
LPDMHFNISHSGRWIVCAVDSKPIGIDIEKMKPGTIDIAKRFFSPTEYSDLQAKHPDQQTDYFYHLWSMKESFIKQAGKG
LSLPLDSFSVRLKDDGHVSIELPDGHEPCFIRTYEADEEYKLAVCAAHPDFCDGIEMKTYEELL

Sequences:

>Translated_224_residues
MKIYGVYMDRPLSAGEEDRMMAAVSAEKREKCRRFYHKEDAHRTLIGDMLIRTAAAKAYGLDPAGISFGVQEYGKPYIPA
LPDMHFNISHSGRWIVCAVDSKPIGIDIEKMKPGTIDIAKRFFSPTEYSDLQAKHPDQQTDYFYHLWSMKESFIKQAGKG
LSLPLDSFSVRLKDDGHVSIELPDGHEPCFIRTYEADEEYKLAVCAAHPDFCDGIEMKTYEELL
>Mature_224_residues
MKIYGVYMDRPLSAGEEDRMMAAVSAEKREKCRRFYHKEDAHRTLIGDMLIRTAAAKAYGLDPAGISFGVQEYGKPYIPA
LPDMHFNISHSGRWIVCAVDSKPIGIDIEKMKPGTIDIAKRFFSPTEYSDLQAKHPDQQTDYFYHLWSMKESFIKQAGKG
LSLPLDSFSVRLKDDGHVSIELPDGHEPCFIRTYEADEEYKLAVCAAHPDFCDGIEMKTYEELL

Specific function: May activate the peptidyl carrier protein (PCP) domains of surfactin synthetase SRF1/2/3 and iturin A synthetase, by transferring the 4'-phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for the coproduction of the lipopeptide a

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

Organism=Homo sapiens, GI20357568, Length=164, Percent_Identity=30.4878048780488, Blast_Score=71, Evalue=6e-13,
Organism=Escherichia coli, GI1789886, Length=91, Percent_Identity=39.5604395604396, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17564880, Length=158, Percent_Identity=29.746835443038, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17569611, Length=170, Percent_Identity=28.2352941176471, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24663173, Length=173, Percent_Identity=30.0578034682081, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR004568 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 25481; Mature: 25481

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYGVYMDRPLSAGEEDRMMAAVSAEKREKCRRFYHKEDAHRTLIGDMLIRTAAAKAYG
CEEEEEEECCCCCCCCCHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LDPAGISFGVQEYGKPYIPALPDMHFNISHSGRWIVCAVDSKPIGIDIEKMKPGTIDIAK
CCCCCCCCCHHHHCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEHHCCCCCHHHHHH
RFFSPTEYSDLQAKHPDQQTDYFYHLWSMKESFIKQAGKGLSLPLDSFSVRLKDDGHVSI
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEE
ELPDGHEPCFIRTYEADEEYKLAVCAAHPDFCDGIEMKTYEELL
ECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHC
>Mature Secondary Structure
MKIYGVYMDRPLSAGEEDRMMAAVSAEKREKCRRFYHKEDAHRTLIGDMLIRTAAAKAYG
CEEEEEEECCCCCCCCCHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LDPAGISFGVQEYGKPYIPALPDMHFNISHSGRWIVCAVDSKPIGIDIEKMKPGTIDIAK
CCCCCCCCCHHHHCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEHHCCCCCHHHHHH
RFFSPTEYSDLQAKHPDQQTDYFYHLWSMKESFIKQAGKGLSLPLDSFSVRLKDDGHVSI
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEE
ELPDGHEPCFIRTYEADEEYKLAVCAAHPDFCDGIEMKTYEELL
ECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8939709 [H]