Click here to switch to the map view.

The map label for this gene is nasF [H]

Identifier: 154684818

GI number: 154684818

Start: 323788

End: 325227

Strand: Reverse

Name: nasF [H]

Synonym: RBAM_003490

Alternate gene names: 154684818

Gene position: 325227-323788 (Counterclockwise)

Preceding gene: 154684819

Following gene: 154684808

Centisome position: 8.3

GC content: 50.21

Gene sequence:

>1440_bases
ATGAAAAACGGAATCGTATATTTTGTCGGTGCCGGACCAGGAGACCCGGGGCTGATCACCGTAAAAGGAAAACAGGCTCT
GGAGAAAGCGGATATTATTTTGTATGACCGGCTCGCAAACCCAAAGTTTCTTGAATATGCCTCACCGGACTGCCGGTTCA
TTTATTGCGGAAAGCTGCCCGACCGCCATTTTATGAAACAGTCTGAGATAAATGCCCTGCTGATTGAAAAAGCGGCGGAG
GGCTTCACCGTTGTCCGCTTGAAAGGCGGAGACCCAAGTGTGTTCGGCAGAGTCGGCGAAGAAGCAGAGGCGCTTCATCA
GCACGGCATCCGCTACGAAATGGTGCCCGGCATCACCTCGGGAATCGCAGCGCCGCTGTATGCGGGAGTCCCCGTCACTC
ATCGTGATTTCGCTTCGTCCTTCGCGATGATCACCGCTCATGATACATCATTGCACGGCAGGCCGAATCTCGACTGGGAA
GGGCTTGCCCGAAGCGTTCAAACGCTTGTGTTTTACATGGGCGTTAAAAATCTGTCATTTATATGCCGTAAGCTTACGGA
GTACGGAAAATCCCCTTCCGTTCCCGTACTCGTCATTCAATGGGGGACGTGGGGGCGGCAGCGGAGCGTTAAAGGAACGC
TTCAGGATATCCATGAAAAAGTTCAAAGCCAAAACATCACCAACCCTGCCATTATCGTCATCGGTGATATTGTCAATTTT
CAAACACACAGCTGGTTTGAAAGCAAGCCGTTCATCGGGCGGCATATCATGGTTGTCACTCATGGCGATGAAGAACATAC
ATTGACAGACAAGCTGCGCGAGTATGGCGCCGATGTTATTGAATGGCCGAAGCGGATCGTTAAAAAAATGCCCGCAAACG
AAAACATCCTGAAACAGATCAGCACCTTTGAACAACTATTTTTCACATCACGCCTTGCGGTATGCGAATTCTTTGACACA
CTGGCTTTCAAGCAGATCGACATCCGCCGGGTGACCGCAAGCCTGAATGCAGCGACTGAGGCCGCAAAGACGGAACTGAC
AAAACGGGGCTTTCTCCTATCCGAATGTCAAATTGATTCTCCGCATTCACTCATTGTAGGAAGCCGTCAAGCAGCAGAAA
ACTTGTCAGACTCAAAATCATGCCCGTTTTATATCACCCATGAACATATCGCAGATGAGCGGTTCACTTCCATGATCGAA
CGCACGATCAGTGAGTCTCCTCTGCAAATGATTATCTGTACTGGCAAAGAATCCGCTCAGCATTTGATCAGCGCCGGAAA
ACAAATCGGCATATCACCGGATCCGTCAGCAAACCGCCCTCCCGTCATCTGCATGGGCGAAGACGTCCCCGCCGGCTGCG
GGTTTACCGCCGTGAAAAATCAGGAACAGTTATTTGCGTTTATACGAAATGAAATGAAAGACATGCTCGTTCACAGCTGA

Upstream 100 bases:

>100_bases
GATTGAAGGAGAGAATGTATACCTCCTGTATTGAGCAAAGACCGGATCACTTTTTCAAGTGGTCCTTCATTTTTTAAACT
GCTGAAGGGATGACGCCATC

Downstream 100 bases:

>100_bases
TACGCCGGAATGCTTTTCTGACCCCCCAGACTCAACATCGGGGGTCAGCCCTTTTTTATTGTGTCACCAATTTTTTATGG
TGAGTCGAATACATCGCCAT

Product: NasF

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKNGIVYFVGAGPGDPGLITVKGKQALEKADIILYDRLANPKFLEYASPDCRFIYCGKLPDRHFMKQSEINALLIEKAAE
GFTVVRLKGGDPSVFGRVGEEAEALHQHGIRYEMVPGITSGIAAPLYAGVPVTHRDFASSFAMITAHDTSLHGRPNLDWE
GLARSVQTLVFYMGVKNLSFICRKLTEYGKSPSVPVLVIQWGTWGRQRSVKGTLQDIHEKVQSQNITNPAIIVIGDIVNF
QTHSWFESKPFIGRHIMVVTHGDEEHTLTDKLREYGADVIEWPKRIVKKMPANENILKQISTFEQLFFTSRLAVCEFFDT
LAFKQIDIRRVTASLNAATEAAKTELTKRGFLLSECQIDSPHSLIVGSRQAAENLSDSKSCPFYITHEHIADERFTSMIE
RTISESPLQMIICTGKESAQHLISAGKQIGISPDPSANRPPVICMGEDVPAGCGFTAVKNQEQLFAFIRNEMKDMLVHS

Sequences:

>Translated_479_residues
MKNGIVYFVGAGPGDPGLITVKGKQALEKADIILYDRLANPKFLEYASPDCRFIYCGKLPDRHFMKQSEINALLIEKAAE
GFTVVRLKGGDPSVFGRVGEEAEALHQHGIRYEMVPGITSGIAAPLYAGVPVTHRDFASSFAMITAHDTSLHGRPNLDWE
GLARSVQTLVFYMGVKNLSFICRKLTEYGKSPSVPVLVIQWGTWGRQRSVKGTLQDIHEKVQSQNITNPAIIVIGDIVNF
QTHSWFESKPFIGRHIMVVTHGDEEHTLTDKLREYGADVIEWPKRIVKKMPANENILKQISTFEQLFFTSRLAVCEFFDT
LAFKQIDIRRVTASLNAATEAAKTELTKRGFLLSECQIDSPHSLIVGSRQAAENLSDSKSCPFYITHEHIADERFTSMIE
RTISESPLQMIICTGKESAQHLISAGKQIGISPDPSANRPPVICMGEDVPAGCGFTAVKNQEQLFAFIRNEMKDMLVHS
>Mature_479_residues
MKNGIVYFVGAGPGDPGLITVKGKQALEKADIILYDRLANPKFLEYASPDCRFIYCGKLPDRHFMKQSEINALLIEKAAE
GFTVVRLKGGDPSVFGRVGEEAEALHQHGIRYEMVPGITSGIAAPLYAGVPVTHRDFASSFAMITAHDTSLHGRPNLDWE
GLARSVQTLVFYMGVKNLSFICRKLTEYGKSPSVPVLVIQWGTWGRQRSVKGTLQDIHEKVQSQNITNPAIIVIGDIVNF
QTHSWFESKPFIGRHIMVVTHGDEEHTLTDKLREYGADVIEWPKRIVKKMPANENILKQISTFEQLFFTSRLAVCEFFDT
LAFKQIDIRRVTASLNAATEAAKTELTKRGFLLSECQIDSPHSLIVGSRQAAENLSDSKSCPFYITHEHIADERFTSMIE
RTISESPLQMIICTGKESAQHLISAGKQIGISPDPSANRPPVICMGEDVPAGCGFTAVKNQEQLFAFIRNEMKDMLVHS

Specific function: Multifunctional Enzyme That Catalyze The Sam-Dependent Methylation Of Uroporphyrinogen III At Position C-2 And C-7 To Form Precorrin-2 And Then Position C-12 Or C-18 To Form Trimethylpyrrocorphin 2. It Also Catalyzes The Conversion Of Precorrin-2 Into Sir

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=246, Percent_Identity=43.4959349593496, Blast_Score=207, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=31.5126050420168, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 53283; Mature: 53283

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNGIVYFVGAGPGDPGLITVKGKQALEKADIILYDRLANPKFLEYASPDCRFIYCGKLP
CCCCEEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCCCEEECCCCCCCEEEECCCC
DRHFMKQSEINALLIEKAAEGFTVVRLKGGDPSVFGRVGEEAEALHQHGIRYEMVPGITS
CHHHHHHHHCCHHEEHHCCCCEEEEEEECCCCHHHHCCCHHHHHHHHCCCEEEECCCCHH
GIAAPLYAGVPVTHRDFASSFAMITAHDTSLHGRPNLDWEGLARSVQTLVFYMGVKNLSF
HHHHHHHCCCCCCHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICRKLTEYGKSPSVPVLVIQWGTWGRQRSVKGTLQDIHEKVQSQNITNPAIIVIGDIVNF
HHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECC
QTHSWFESKPFIGRHIMVVTHGDEEHTLTDKLREYGADVIEWPKRIVKKMPANENILKQI
CCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
STFEQLFFTSRLAVCEFFDTLAFKQIDIRRVTASLNAATEAAKTELTKRGFLLSECQIDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
PHSLIVGSRQAAENLSDSKSCPFYITHEHIADERFTSMIERTISESPLQMIICTGKESAQ
CCEEEEECHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
HLISAGKQIGISPDPSANRPPVICMGEDVPAGCGFTAVKNQEQLFAFIRNEMKDMLVHS
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNGIVYFVGAGPGDPGLITVKGKQALEKADIILYDRLANPKFLEYASPDCRFIYCGKLP
CCCCEEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCCCEEECCCCCCCEEEECCCC
DRHFMKQSEINALLIEKAAEGFTVVRLKGGDPSVFGRVGEEAEALHQHGIRYEMVPGITS
CHHHHHHHHCCHHEEHHCCCCEEEEEEECCCCHHHHCCCHHHHHHHHCCCEEEECCCCHH
GIAAPLYAGVPVTHRDFASSFAMITAHDTSLHGRPNLDWEGLARSVQTLVFYMGVKNLSF
HHHHHHHCCCCCCHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICRKLTEYGKSPSVPVLVIQWGTWGRQRSVKGTLQDIHEKVQSQNITNPAIIVIGDIVNF
HHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECC
QTHSWFESKPFIGRHIMVVTHGDEEHTLTDKLREYGADVIEWPKRIVKKMPANENILKQI
CCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
STFEQLFFTSRLAVCEFFDTLAFKQIDIRRVTASLNAATEAAKTELTKRGFLLSECQIDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
PHSLIVGSRQAAENLSDSKSCPFYITHEHIADERFTSMIERTISESPLQMIICTGKESAQ
CCEEEEECHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
HLISAGKQIGISPDPSANRPPVICMGEDVPAGCGFTAVKNQEQLFAFIRNEMKDMLVHS
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]