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The map label for this gene is asbF [H]

Identifier: 154684808

GI number: 154684808

Start: 311970

End: 312758

Strand: Reverse

Name: asbF [H]

Synonym: RBAM_003390

Alternate gene names: 154684808

Gene position: 312758-311970 (Counterclockwise)

Preceding gene: 154684818

Following gene: 154684807

Centisome position: 7.98

GC content: 47.53

Gene sequence:

>789_bases
ATGAAACTGAGTTTTTGCACGACAGGATTTAAGAAAAAGCATATTTTCGACATCATTTCTTTCGCGAAGGAGCACTCCTT
TGAGGGAATCGAACTGTGGTACGGACACGCTAAACAATATATAGAGGAAATCGGCCCGCTCGACAGGCTTTATCAGCAGC
TTCAGGAGGAAAGCATCGGGGTGCCTGCCGTCAGTTTGTACACTGACTTCGCCACTCAGGATGAAAGGCAGGCGTTAAAG
GATGTTCAAAGGGCATTAAGCGCGGCACGACAGCTGCACAGTCCCTTGATCAGAGTCTTTGCCGGCAATCTTCCTTCGCA
CCGGACGTCACCGGAAAAGTGGCAACGTGCGGTTGATCAATTTCAGCAGGCGGCACGGCTCGCAGATGACTATGAAGTCG
ATATCGGGCTTGAGATTCATTACGATACGTACGCGGATACACCGGAGGCGGCGCTGGCATTTTTGGAGGATGTCGGACAT
CCGCGGGTCCGCACCATTTTTGACGGCTCCAACCTACACGTCAATCACGTTGACCAAATGTCCGCTTTGGAAAAACTGTA
TCATACCGTCAGCCACATTCATCTGAAAAACTATCACTGGAATTTTGACAACTGGTATCTCAGCAAGCCAACTCCGATTT
TTCAAGGAGATCTCGATCAAAAAGCATTTTTAAAAGAGCTGATGAACAGACAATATGACGGTTTTGTTTCTCTTGAGTAT
TTTGGAGAACATGCCTTTACGCATATAAAGGAATCGCTCCGCCAATATACGTCTGTTTTTTCCCGCTGA

Upstream 100 bases:

>100_bases
CACCCCGCTTATAAAAAGTGTCTGCCCTTTTCACCTGTTTATCCGTATCGCACCCGGGGAAAAAGTACACATATGATAGA
CAGTTACGGGAGGATTATCG

Downstream 100 bases:

>100_bases
CTCTTCGATCGAAGGGTCTTTTTTTGTATCATATAGAAAAAGGGGGATATCATGATGATCATTTGGATAAATGGGGCTTT
CGGTTCCGGAAAAACACAAA

Product: hypothetical protein

Products: NA

Alternate protein names: 3-DHS dehydratase; DHSase; Petrobactin biosynthesis protein AsbF [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKLSFCTTGFKKKHIFDIISFAKEHSFEGIELWYGHAKQYIEEIGPLDRLYQQLQEESIGVPAVSLYTDFATQDERQALK
DVQRALSAARQLHSPLIRVFAGNLPSHRTSPEKWQRAVDQFQQAARLADDYEVDIGLEIHYDTYADTPEAALAFLEDVGH
PRVRTIFDGSNLHVNHVDQMSALEKLYHTVSHIHLKNYHWNFDNWYLSKPTPIFQGDLDQKAFLKELMNRQYDGFVSLEY
FGEHAFTHIKESLRQYTSVFSR

Sequences:

>Translated_262_residues
MKLSFCTTGFKKKHIFDIISFAKEHSFEGIELWYGHAKQYIEEIGPLDRLYQQLQEESIGVPAVSLYTDFATQDERQALK
DVQRALSAARQLHSPLIRVFAGNLPSHRTSPEKWQRAVDQFQQAARLADDYEVDIGLEIHYDTYADTPEAALAFLEDVGH
PRVRTIFDGSNLHVNHVDQMSALEKLYHTVSHIHLKNYHWNFDNWYLSKPTPIFQGDLDQKAFLKELMNRQYDGFVSLEY
FGEHAFTHIKESLRQYTSVFSR
>Mature_262_residues
MKLSFCTTGFKKKHIFDIISFAKEHSFEGIELWYGHAKQYIEEIGPLDRLYQQLQEESIGVPAVSLYTDFATQDERQALK
DVQRALSAARQLHSPLIRVFAGNLPSHRTSPEKWQRAVDQFQQAARLADDYEVDIGLEIHYDTYADTPEAALAFLEDVGH
PRVRTIFDGSNLHVNHVDQMSALEKLYHTVSHIHLKNYHWNFDNWYLSKPTPIFQGDLDQKAFLKELMNRQYDGFVSLEY
FGEHAFTHIKESLRQYTSVFSR

Specific function: Converts 3-dehydroshikimate to protocatechuate (3,4- dihydroxybenzoate), a biosynthetic unit required for the biosynthesis of petrobactin which is a virulence-associated siderophore produced by B.anthracis [H]

COG id: COG1082

COG function: function code G; Sugar phosphate isomerases/epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =4.2.1.118 [H]

Molecular weight: Translated: 30514; Mature: 30514

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSFCTTGFKKKHIFDIISFAKEHSFEGIELWYGHAKQYIEEIGPLDRLYQQLQEESIG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
VPAVSLYTDFATQDERQALKDVQRALSAARQLHSPLIRVFAGNLPSHRTSPEKWQRAVDQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FQQAARLADDYEVDIGLEIHYDTYADTPEAALAFLEDVGHPRVRTIFDGSNLHVNHVDQM
HHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEECHHHHH
SALEKLYHTVSHIHLKNYHWNFDNWYLSKPTPIFQGDLDQKAFLKELMNRQYDGFVSLEY
HHHHHHHHHHHHHHHHCEECCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHH
FGEHAFTHIKESLRQYTSVFSR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLSFCTTGFKKKHIFDIISFAKEHSFEGIELWYGHAKQYIEEIGPLDRLYQQLQEESIG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
VPAVSLYTDFATQDERQALKDVQRALSAARQLHSPLIRVFAGNLPSHRTSPEKWQRAVDQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FQQAARLADDYEVDIGLEIHYDTYADTPEAALAFLEDVGHPRVRTIFDGSNLHVNHVDQM
HHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEECHHHHH
SALEKLYHTVSHIHLKNYHWNFDNWYLSKPTPIFQGDLDQKAFLKELMNRQYDGFVSLEY
HHHHHHHHHHHHHHHHCEECCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHH
FGEHAFTHIKESLRQYTSVFSR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]