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The map label for this gene is mdr [H]

Identifier: 154684799

GI number: 154684799

Start: 304177

End: 305709

Strand: Reverse

Name: mdr [H]

Synonym: RBAM_003300

Alternate gene names: 154684799

Gene position: 305709-304177 (Counterclockwise)

Preceding gene: 154684803

Following gene: 154684794

Centisome position: 7.8

GC content: 49.25

Gene sequence:

>1533_bases
ATGGAACAAACAGCCGCGAAGGACGCCTCGACGAAGTTTGTCGTCATCGGTCTTTTGCTGGGCATTTTAATGTCGGCTAT
GGATAATACAATCGTTGCCACTGCGATGGGAAATATCGTGGCGGACCTCGGAAGTTTCGATAAGTTCGCGTGGGTGACGG
CATCATACATGGTAGCCGTTATGGCCGGTATGCCGATTTACGGAAAACTGTCCGATATGTACGGACGCAAACGTTTCTTT
TTATTCGGTCTTATTCTTTTCTTAATCGGATCCGCATTATGCGGAATCGCCCAAACAATGGATCAGCTCATTATTTACCG
CGTCATTCAGGGAATCGGCGGAGGCGCGCTGATGCCGATCGCCTTCACGATCATTTTTGATTTATTTCCGCCGGAAAAAC
GCGGAAAAATGTCGGGCATGTTCGGTGCCGTTTTCGGCCTGTCCAGCGTTCTCGGGCCGCTTTTAGGCGCGCTCATTACC
GACTCCATCAGCTGGCATTGGGTGTTTTACATCAACGTGCCGATCGGTATTTTATCATTGTTTTTCATTCTTCGTTATTA
TAAAGAATCCCTTGAACACAAGAAGCAGAAAATCGACTGGGCCGGCGCAATCACGCTGGTGGTTTCCATTGTCGGTCTGA
TGTTCGCTCTTGAGCTCGGCGGAAAAACGTATGATTGGAACTCCGTGCAGATCATCGGTCTGTTCGCTGTGTTCGCCGTG
TTCTTTATCGCCTTTTTTATTGTGGAAAGAAAAGCGGAAGAACCGATTATCTCATTTTGGATGTTTAAAAACCGTTTATT
TGCTACGGCACAGATTCTGGCGTTCCTTTACGGAGCCACGTTCGTCATTTTAGCGGTGTTTATCCCGATCTTCGTTCAGG
CTGTGTACGGAAGCACCGCGACAAGCGCGGGCTTTATTTTGACGCCGATGATGATCGGTTCTGTTATCGGAAGTATGATC
GGCGGAATTTTCCAGACGAAAGTGCGGTTCCGCACTTTAATGCTGATTTCCGTGGTCGCTTTCTTTATCGGAATGCTGCT
GCTTTCCAATATGACACCGGATACGGCGCGTACGATGCTGACGGTATTCATGCTGATCTCAGGATTCGGCGTCGGCTTCA
ACTTCTCACTGCTGCCGGCTGCGTCCATGAACGATCTGGAGCCGCGTTACCGCGGAAGCGCCAACTCAACAAACTCGTTT
TTGAGATCATTCGGTATGACGCTCGGTGTGACGATTTTCGGAACCATCCAGACGAATGTGTTTACCAACAAGCTCACAGA
TTCATTCAGCGGCATGAAAGGAGCGGGCGGCGCGATGCAGAACATCGGAAATCCGCAGGAGATTTTCCAGGCCGGCACGC
GCGCTCATATTCCGCCGCACATTCTGGATCGCATTATTGACGCCATGTCGCAAAGCATCACATATGTATTTACGCTTGCG
CTGGCGCCGATCGTCATTGCGGCCGTTACCATTTTGTTTATGGGAAAAGCGAGAGTAAAAACCTCTCAGGAAATGGTGAA
AAAAGCGAATTAA

Upstream 100 bases:

>100_bases
CTATAAAATCAATTTATCTTGACGATCAAGATAATTTTCGGCAAATCTAATATGATCATAGCAAATTTAATGATCAAAAG
TAAGTATGGGAGGAAAAAGC

Downstream 100 bases:

>100_bases
AAAAAGCAGTACATGGCGTTTGCCATGTACTGCTTTTTTGTTTACTCTGCGAAATGCGGAGCGAGAATATCTTTCAGCAC
GTTTGCGCTGCGGCTGAATT

Product: major facilitator superfamily transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MEQTAAKDASTKFVVIGLLLGILMSAMDNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFF
LFGLILFLIGSALCGIAQTMDQLIIYRVIQGIGGGALMPIAFTIIFDLFPPEKRGKMSGMFGAVFGLSSVLGPLLGALIT
DSISWHWVFYINVPIGILSLFFILRYYKESLEHKKQKIDWAGAITLVVSIVGLMFALELGGKTYDWNSVQIIGLFAVFAV
FFIAFFIVERKAEEPIISFWMFKNRLFATAQILAFLYGATFVILAVFIPIFVQAVYGSTATSAGFILTPMMIGSVIGSMI
GGIFQTKVRFRTLMLISVVAFFIGMLLLSNMTPDTARTMLTVFMLISGFGVGFNFSLLPAASMNDLEPRYRGSANSTNSF
LRSFGMTLGVTIFGTIQTNVFTNKLTDSFSGMKGAGGAMQNIGNPQEIFQAGTRAHIPPHILDRIIDAMSQSITYVFTLA
LAPIVIAAVTILFMGKARVKTSQEMVKKAN

Sequences:

>Translated_510_residues
MEQTAAKDASTKFVVIGLLLGILMSAMDNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFF
LFGLILFLIGSALCGIAQTMDQLIIYRVIQGIGGGALMPIAFTIIFDLFPPEKRGKMSGMFGAVFGLSSVLGPLLGALIT
DSISWHWVFYINVPIGILSLFFILRYYKESLEHKKQKIDWAGAITLVVSIVGLMFALELGGKTYDWNSVQIIGLFAVFAV
FFIAFFIVERKAEEPIISFWMFKNRLFATAQILAFLYGATFVILAVFIPIFVQAVYGSTATSAGFILTPMMIGSVIGSMI
GGIFQTKVRFRTLMLISVVAFFIGMLLLSNMTPDTARTMLTVFMLISGFGVGFNFSLLPAASMNDLEPRYRGSANSTNSF
LRSFGMTLGVTIFGTIQTNVFTNKLTDSFSGMKGAGGAMQNIGNPQEIFQAGTRAHIPPHILDRIIDAMSQSITYVFTLA
LAPIVIAAVTILFMGKARVKTSQEMVKKAN
>Mature_510_residues
MEQTAAKDASTKFVVIGLLLGILMSAMDNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFF
LFGLILFLIGSALCGIAQTMDQLIIYRVIQGIGGGALMPIAFTIIFDLFPPEKRGKMSGMFGAVFGLSSVLGPLLGALIT
DSISWHWVFYINVPIGILSLFFILRYYKESLEHKKQKIDWAGAITLVVSIVGLMFALELGGKTYDWNSVQIIGLFAVFAV
FFIAFFIVERKAEEPIISFWMFKNRLFATAQILAFLYGATFVILAVFIPIFVQAVYGSTATSAGFILTPMMIGSVIGSMI
GGIFQTKVRFRTLMLISVVAFFIGMLLLSNMTPDTARTMLTVFMLISGFGVGFNFSLLPAASMNDLEPRYRGSANSTNSF
LRSFGMTLGVTIFGTIQTNVFTNKLTDSFSGMKGAGGAMQNIGNPQEIFQAGTRAHIPPHILDRIIDAMSQSITYVFTLA
LAPIVIAAVTILFMGKARVKTSQEMVKKAN

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=445, Percent_Identity=31.0112359550562, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788710, Length=412, Percent_Identity=30.5825242718447, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1790195, Length=400, Percent_Identity=29.25, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87081983, Length=319, Percent_Identity=25.705329153605, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788392, Length=414, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790146, Length=163, Percent_Identity=30.0613496932515, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788509, Length=144, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI48994889, Length=162, Percent_Identity=28.3950617283951, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=466, Percent_Identity=25.7510729613734, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323735, Length=545, Percent_Identity=26.4220183486239, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321663, Length=538, Percent_Identity=24.1635687732342, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6319783, Length=384, Percent_Identity=26.3020833333333, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319770, Length=386, Percent_Identity=26.6839378238342, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325455, Length=451, Percent_Identity=22.8381374722838, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324954, Length=395, Percent_Identity=24.0506329113924, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323520, Length=424, Percent_Identity=27.122641509434, Blast_Score=90, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55630; Mature: 55630

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]