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The map label for this gene is yoaI [H]

Identifier: 154684732

GI number: 154684732

Start: 235061

End: 236182

Strand: Reverse

Name: yoaI [H]

Synonym: RBAM_002630

Alternate gene names: 154684732

Gene position: 236182-235061 (Counterclockwise)

Preceding gene: 154684733

Following gene: 154684730

Centisome position: 6.03

GC content: 47.24

Gene sequence:

>1122_bases
ATGAATACCGTGCTGATGAGCTTCGCCTCCTCCGCCGCGCTTTTGAAAGACCGCGATCATTGTTTTCCGGAACATGTTCA
AGCGCTGTACGAACGCGCTGCTGCACAGGATCTTTCTTTTACGCACACGTTTATCACCCCTCAGGTGAATCGTTCACAAA
TGGGTGTAAACGTATCGCCGACACCTATCTCGGCAAAAGTCGTCGATAAAAATGAAAAAGGCCTCATCATACACGGCGCC
CGGATTTTAGCCACACAAGGCGGCCTGACGGATGAAGTGCTCGTGGTTTCTCTGCCTAAAATGTATTTTGAATCGGATGA
AGCGTTCGCTTTTTCAATCCCTTCCAATACAAAGGGAGTAAAATTCATCTGCAGGGAATCTTTCGTACTCGGAAAATCAT
CGTTTGATTATCCGCTCAGCTCAAGATATGAAGAAATGGATTCTATCGTTGTATTTGATCACGTTTTGGTCCCTTGGGAA
CGCGTATTTTTTTATAATAATTACGAGGCGGCTTTTGATTTTAATATGAAAAGCTCTTTTAACGCCTTCGCATTTCATCA
AGTTATCACGAGACAAATTGTAAAGACGGAGTTTCTCCTGGGCCTCGCCCAAACGCTTGTCGAAGCGATAGATGTGGGCG
AATATCAGCATATTCAGGCTAAGATGGCTGAAATCATCACCGGTCTGGAAACGATGAAAGCCCTTTTGGAAAAGTCGGAG
AATGATGCGGAAACAGATGAATGGGGTTATATGCGTCCGAGTGTCACTCCGCTTCGTGTGGTCAGCGCGATTTTCCCCGA
CATCTACCCGCGCTTCACGGAAATCATTCAGCTGATCGGGGCAAGCGGAATGGTGACTTTGCCGACTGAAAAAGCGTTCC
GTTCTGACATCAGAGGAGACCTTGACCAATATTTGCAGGCGGCCGAATTGAACGCTGAGGACCGGGTAAAAGTGTTCCGC
CTCGCATGGGATTTGACCATGAGCCCCTTCGGAACAAGACAGACACAATATGAGCGGTACTTCTTCGGTGACCCCGTCCG
CTTAACCAGCCATCTGTACAGAAGCTATCCGAAAGAAGAACAGACCGCGGTCATCAAGGAATTCCTGCACATTCATAAAT
AA

Upstream 100 bases:

>100_bases
CCTGCAGCCGAAAACGAAAGAAGACGTAAAAAGAAGAAGAAACATGTTCGAACATTGGGCCAGACACACGCACGGAATGA
TGGGACGAAGCCCGGATTAT

Downstream 100 bases:

>100_bases
AACAAAGCAGAGAGCGCATCTCTGCTTATGATAGCTGGATCGGCTGCCGCCGATCCTATTTTTTATTTTGGCTGATCACC
ACCATCGTAATATGCGATAA

Product: YoaI

Products: NA

Alternate protein names: Putative 4-HPA 3-hydroxylase; Putative 4-hydroxyphenylacetate-3-hydroxylase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNTVLMSFASSAALLKDRDHCFPEHVQALYERAAAQDLSFTHTFITPQVNRSQMGVNVSPTPISAKVVDKNEKGLIIHGA
RILATQGGLTDEVLVVSLPKMYFESDEAFAFSIPSNTKGVKFICRESFVLGKSSFDYPLSSRYEEMDSIVVFDHVLVPWE
RVFFYNNYEAAFDFNMKSSFNAFAFHQVITRQIVKTEFLLGLAQTLVEAIDVGEYQHIQAKMAEIITGLETMKALLEKSE
NDAETDEWGYMRPSVTPLRVVSAIFPDIYPRFTEIIQLIGASGMVTLPTEKAFRSDIRGDLDQYLQAAELNAEDRVKVFR
LAWDLTMSPFGTRQTQYERYFFGDPVRLTSHLYRSYPKEEQTAVIKEFLHIHK

Sequences:

>Translated_373_residues
MNTVLMSFASSAALLKDRDHCFPEHVQALYERAAAQDLSFTHTFITPQVNRSQMGVNVSPTPISAKVVDKNEKGLIIHGA
RILATQGGLTDEVLVVSLPKMYFESDEAFAFSIPSNTKGVKFICRESFVLGKSSFDYPLSSRYEEMDSIVVFDHVLVPWE
RVFFYNNYEAAFDFNMKSSFNAFAFHQVITRQIVKTEFLLGLAQTLVEAIDVGEYQHIQAKMAEIITGLETMKALLEKSE
NDAETDEWGYMRPSVTPLRVVSAIFPDIYPRFTEIIQLIGASGMVTLPTEKAFRSDIRGDLDQYLQAAELNAEDRVKVFR
LAWDLTMSPFGTRQTQYERYFFGDPVRLTSHLYRSYPKEEQTAVIKEFLHIHK
>Mature_373_residues
MNTVLMSFASSAALLKDRDHCFPEHVQALYERAAAQDLSFTHTFITPQVNRSQMGVNVSPTPISAKVVDKNEKGLIIHGA
RILATQGGLTDEVLVVSLPKMYFESDEAFAFSIPSNTKGVKFICRESFVLGKSSFDYPLSSRYEEMDSIVVFDHVLVPWE
RVFFYNNYEAAFDFNMKSSFNAFAFHQVITRQIVKTEFLLGLAQTLVEAIDVGEYQHIQAKMAEIITGLETMKALLEKSE
NDAETDEWGYMRPSVTPLRVVSAIFPDIYPRFTEIIQLIGASGMVTLPTEKAFRSDIRGDLDQYLQAAELNAEDRVKVFR
LAWDLTMSPFGTRQTQYERYFFGDPVRLTSHLYRSYPKEEQTAVIKEFLHIHK

Specific function: Catalyzs the hydroxylation of 4-hydroxyphenylacetic acid (4HPA), leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: NA

Molecular weight: Translated: 42501; Mature: 42501

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVLMSFASSAALLKDRDHCFPEHVQALYERAAAQDLSFTHTFITPQVNRSQMGVNVSP
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCC
TPISAKVVDKNEKGLIIHGARILATQGGLTDEVLVVSLPKMYFESDEAFAFSIPSNTKGV
CCCEEEEECCCCCEEEEECCEEEEECCCCCCCHHEEEHHHHHHCCCCEEEEECCCCCCCC
KFICRESFVLGKSSFDYPLSSRYEEMDSIVVFDHVLVPWERVFFYNNYEAAFDFNMKSSF
EEEEHHHHEECCCCCCCCHHHHHHHHCCEEEEHHHHCCHHHEEEECCCCEEEECCCCCCC
NAFAFHQVITRQIVKTEFLLGLAQTLVEAIDVGEYQHIQAKMAEIITGLETMKALLEKSE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NDAETDEWGYMRPSVTPLRVVSAIFPDIYPRFTEIIQLIGASGMVTLPTEKAFRSDIRGD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
LDQYLQAAELNAEDRVKVFRLAWDLTMSPFGTRQTQYERYFFGDPVRLTSHLYRSYPKEE
HHHHHHHHHCCCHHHHEEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCHH
QTAVIKEFLHIHK
HHHHHHHHHHHCC
>Mature Secondary Structure
MNTVLMSFASSAALLKDRDHCFPEHVQALYERAAAQDLSFTHTFITPQVNRSQMGVNVSP
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCC
TPISAKVVDKNEKGLIIHGARILATQGGLTDEVLVVSLPKMYFESDEAFAFSIPSNTKGV
CCCEEEEECCCCCEEEEECCEEEEECCCCCCCHHEEEHHHHHHCCCCEEEEECCCCCCCC
KFICRESFVLGKSSFDYPLSSRYEEMDSIVVFDHVLVPWERVFFYNNYEAAFDFNMKSSF
EEEEHHHHEECCCCCCCCHHHHHHHHCCEEEEHHHHCCHHHEEEECCCCEEEECCCCCCC
NAFAFHQVITRQIVKTEFLLGLAQTLVEAIDVGEYQHIQAKMAEIITGLETMKALLEKSE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NDAETDEWGYMRPSVTPLRVVSAIFPDIYPRFTEIIQLIGASGMVTLPTEKAFRSDIRGD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
LDQYLQAAELNAEDRVKVFRLAWDLTMSPFGTRQTQYERYFFGDPVRLTSHLYRSYPKEE
HHHHHHHHHCCCHHHHEEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCHH
QTAVIKEFLHIHK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]