| Definition | Bacillus amyloliquefaciens FZB42, complete genome. |
|---|---|
| Accession | NC_009725 |
| Length | 3,918,589 |
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The map label for this gene is kdgR1 [H]
Identifier: 154684712
GI number: 154684712
Start: 215385
End: 216230
Strand: Reverse
Name: kdgR1 [H]
Synonym: RBAM_002430
Alternate gene names: 154684712
Gene position: 216230-215385 (Counterclockwise)
Preceding gene: 154684713
Following gene: 154684704
Centisome position: 5.52
GC content: 50.95
Gene sequence:
>846_bases ATGATGACTGGAGCAATTCCGCAAAAGAAACTGCTGGATGGGACAAGCATTCCCGCGATCGGCTTCGGTACATACGCTCT TAACGGAAACAACGGCGTTCATGCCATTACCAGCGCCATTGATATCGGCTACCGTCTGATTGACACCGCTTATAACTATG AAAATGAAGGGACGGTCGGAGAAGCCATCAAGAGAAGCTCTGTTCCGAGAGAACAGCTATACGTGACGTCCAAGCTGCCC GGACGCTACCATGACTATGACAAAGCTGTACAAACCATACAAGAATCTTTATACCGCGCCGGTCTGGACTACTATGACCT GTATCTCGTCCATTGGCCGAACCCGAAACAAGACCGCTACACGGAAGCATGGCAGGCACTGATTGATGCGAAAAAATGGG GTCTGATCCGATCCATCGGCGTCTGCAATTTCCTGCCTGAGCATATCGAACGGCTCGAACGTGAAACCGGCGTAAAGCCA GGCATCAACCAAATTGAACTGCATCCCGCTTTTAATCAGCAGCAGCAGCGCCAATGGCACGAAGAACAGGGCATCGTGAC AGAATCATGGAGTCCGTTATCCCGCGCCGGCGAAGTATTGAACGATCAAAAACTCGGACGGATTGCGGACAAATACGGCA AAACCATTTCCCAAGTCATTTTACGCTGGCATATCCAGCTGGGCGCCATCCCGATTCCAAAATCAGCATCGCCCGAGCGC CAGCTTGAAAACATCTCCGTTTTTGATTTCTCTCTTGATGAAAACGACATGAGCATTATCGACTCGCTTACCCGCCCCGA CGGACGCATAAACGGCCAAGACCCGGCGGAGTATGAGGAGTTTTAA
Upstream 100 bases:
>100_bases TCTCCGTCAGCTTTTTTCATGTGCGGATCATCTCATTTCCTTCCATTATTATGTTTCATTTGTAAGAATAATGGTAAAGT AGAAAACAAGGAGGGATTTA
Downstream 100 bases:
>100_bases GTAAACGCATAAAGAAAAGCTGACGGCATCATTTGTCAGCTTTTTTCTCATTTTATATACGCACACCCAGCTCCCGTTTC CATTCAGAAAGCCAATCTGT
Product: KdgR1
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 281; Mature: 281
Protein sequence:
>281_residues MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVGEAIKRSSVPREQLYVTSKLP GRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRYTEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKP GINQIELHPAFNQQQQRQWHEEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF
Sequences:
>Translated_281_residues MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVGEAIKRSSVPREQLYVTSKLP GRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRYTEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKP GINQIELHPAFNQQQQRQWHEEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF >Mature_281_residues MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVGEAIKRSSVPREQLYVTSKLP GRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRYTEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKP GINQIELHPAFNQQQQRQWHEEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI223468663, Length=284, Percent_Identity=35.9154929577465, Blast_Score=174, Evalue=1e-43, Organism=Homo sapiens, GI291291012, Length=264, Percent_Identity=35.9848484848485, Blast_Score=160, Evalue=2e-39, Organism=Homo sapiens, GI24497577, Length=287, Percent_Identity=32.404181184669, Blast_Score=158, Evalue=6e-39, Organism=Homo sapiens, GI5174391, Length=287, Percent_Identity=32.404181184669, Blast_Score=158, Evalue=6e-39, Organism=Homo sapiens, GI5174695, Length=302, Percent_Identity=33.7748344370861, Blast_Score=156, Evalue=2e-38, Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=34.7222222222222, Blast_Score=154, Evalue=1e-37, Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=32.2033898305085, Blast_Score=152, Evalue=3e-37, Organism=Homo sapiens, GI5453543, Length=293, Percent_Identity=33.7883959044369, Blast_Score=149, Evalue=4e-36, Organism=Homo sapiens, GI310109920, Length=293, Percent_Identity=33.7883959044369, Blast_Score=148, Evalue=5e-36, Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=34.9090909090909, Blast_Score=148, Evalue=5e-36, Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=33.1058020477816, Blast_Score=147, Evalue=7e-36, Organism=Homo sapiens, GI45446745, Length=293, Percent_Identity=33.4470989761092, Blast_Score=147, Evalue=8e-36, Organism=Homo sapiens, GI4503285, Length=293, Percent_Identity=33.4470989761092, Blast_Score=147, Evalue=8e-36, Organism=Homo sapiens, GI24497585, Length=293, Percent_Identity=33.4470989761092, Blast_Score=147, Evalue=1e-35, Organism=Homo sapiens, GI300116273, Length=284, Percent_Identity=33.0985915492958, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI300116271, Length=285, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=5e-29, Organism=Homo sapiens, GI310109924, Length=148, Percent_Identity=33.1081081081081, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI310109926, Length=124, Percent_Identity=39.5161290322581, Blast_Score=77, Evalue=1e-14, Organism=Homo sapiens, GI207028673, Length=124, Percent_Identity=38.7096774193548, Blast_Score=77, Evalue=2e-14, Organism=Escherichia coli, GI87082198, Length=270, Percent_Identity=46.6666666666667, Blast_Score=248, Evalue=2e-67, Organism=Escherichia coli, GI1786400, Length=261, Percent_Identity=37.1647509578544, Blast_Score=167, Evalue=5e-43, Organism=Escherichia coli, GI1788081, Length=276, Percent_Identity=28.6231884057971, Blast_Score=93, Evalue=2e-20, Organism=Escherichia coli, GI48994888, Length=239, Percent_Identity=25.9414225941423, Blast_Score=70, Evalue=2e-13, Organism=Caenorhabditis elegans, GI17537075, Length=288, Percent_Identity=38.8888888888889, Blast_Score=177, Evalue=6e-45, Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=38.5416666666667, Blast_Score=176, Evalue=1e-44, Organism=Caenorhabditis elegans, GI71998625, Length=272, Percent_Identity=34.9264705882353, Blast_Score=160, Evalue=8e-40, Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=33.4558823529412, Blast_Score=155, Evalue=3e-38, Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=32, Blast_Score=151, Evalue=4e-37, Organism=Caenorhabditis elegans, GI17537079, Length=291, Percent_Identity=31.9587628865979, Blast_Score=149, Evalue=2e-36, Organism=Caenorhabditis elegans, GI17566692, Length=296, Percent_Identity=29.7297297297297, Blast_Score=148, Evalue=3e-36, Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=33.9483394833948, Blast_Score=143, Evalue=8e-35, Organism=Caenorhabditis elegans, GI17550248, Length=304, Percent_Identity=32.5657894736842, Blast_Score=137, Evalue=5e-33, Organism=Caenorhabditis elegans, GI17561300, Length=252, Percent_Identity=32.9365079365079, Blast_Score=133, Evalue=9e-32, Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=29.0566037735849, Blast_Score=127, Evalue=5e-30, Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=2e-23, Organism=Saccharomyces cerevisiae, GI6320576, Length=288, Percent_Identity=38.5416666666667, Blast_Score=177, Evalue=1e-45, Organism=Saccharomyces cerevisiae, GI6324694, Length=285, Percent_Identity=34.3859649122807, Blast_Score=155, Evalue=6e-39, Organism=Saccharomyces cerevisiae, GI6322556, Length=261, Percent_Identity=31.8007662835249, Blast_Score=142, Evalue=7e-35, Organism=Saccharomyces cerevisiae, GI6319625, Length=283, Percent_Identity=33.9222614840989, Blast_Score=132, Evalue=9e-32, Organism=Saccharomyces cerevisiae, GI6321896, Length=312, Percent_Identity=31.0897435897436, Blast_Score=130, Evalue=3e-31, Organism=Saccharomyces cerevisiae, GI6320079, Length=278, Percent_Identity=29.8561151079137, Blast_Score=110, Evalue=2e-25, Organism=Drosophila melanogaster, GI24657054, Length=292, Percent_Identity=39.3835616438356, Blast_Score=202, Evalue=2e-52, Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=38.1443298969072, Blast_Score=197, Evalue=8e-51, Organism=Drosophila melanogaster, GI20129731, Length=282, Percent_Identity=35.4609929078014, Blast_Score=184, Evalue=4e-47, Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=35.7894736842105, Blast_Score=182, Evalue=2e-46, Organism=Drosophila melanogaster, GI45553081, Length=280, Percent_Identity=32.8571428571429, Blast_Score=156, Evalue=1e-38, Organism=Drosophila melanogaster, GI24644950, Length=290, Percent_Identity=32.4137931034483, Blast_Score=146, Evalue=2e-35, Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=31.0580204778157, Blast_Score=139, Evalue=2e-33, Organism=Drosophila melanogaster, GI221378297, Length=310, Percent_Identity=30.3225806451613, Blast_Score=138, Evalue=4e-33, Organism=Drosophila melanogaster, GI24662785, Length=277, Percent_Identity=35.3790613718412, Blast_Score=134, Evalue=5e-32, Organism=Drosophila melanogaster, GI24662781, Length=279, Percent_Identity=34.7670250896057, Blast_Score=133, Evalue=1e-31, Organism=Drosophila melanogaster, GI281366140, Length=245, Percent_Identity=32.6530612244898, Blast_Score=133, Evalue=2e-31,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 31903; Mature: 31903
Theoretical pI: Translated: 5.21; Mature: 5.21
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.4 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 1.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVG CCCCCCCHHHHCCCCCCCEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH EAIKRSSVPREQLYVTSKLPGRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRY HHHHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHH TEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKPGINQIELHPAFNQQQQRQWH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH EEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF HHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHCCCC >Mature Secondary Structure MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVG CCCCCCCHHHHCCCCCCCEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH EAIKRSSVPREQLYVTSKLPGRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRY HHHHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHH TEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKPGINQIELHPAFNQQQQRQWH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH EEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF HHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA